Lactulose Crystals Beneficially Affect Community Composition Along Entire Human Colon in Vitro, Resulting in Donor-Dependent Prebiotic Effects at Metabolic Level

Cindy Duysburgh; Pieter Van Den Abbeele; Melanie Bothe; John Stover; Angelika Kuchinka-Koch; Susann Schwejda-Guettes; Massimo Marzorati

doi:doi:10.11648/j.ijnfs.20190801.12

Lactulose Crystals Beneficially Affect Community Composition Along Entire Human Colon in Vitro, Resulting in Donor-Dependent Prebiotic Effects at Metabolic Level

Cindy Duysburgh, Pieter Van Den Abbeele, Melanie Bothe, John Stover, Angelika Kuchinka-Koch, Susann Schwejda-Guettes, Massimo Marzorati

Published in International Journal of Nutrition and Food Sciences (Volume 8, Issue 1)

Received: 12 February 2019 Accepted: 20 March 2019 Published: 29 April 2019

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Abstract

A validated in vitro gut model (i.e. SHIME®) was used to assess the effect of repeated daily administration of lactulose on microbial metabolic activity and community composition in different colonic areas, with the focus on inter-individual differences among three human subjects. An initial rise in acetate and lactate levels was observed in the proximal colon after lactulose administration, which could be linked to an overall strong bifidogenic effect as well as higher Lactobacilli levels in donors 2 and 3. Particularly two operational taxonomic units (OTUs) related to Bifidobacterium adolescentis and Bifidobacterium longum increased with lactulose addition. The enhanced acetate and lactate production subsequently stimulated microbial species involved in cross-feeding interactions, resulting in the donor-dependent production of propionate and/or butyrate. Additionally, a reduction in markers of proteolytic fermentation was detected upon lactulose supplementation. A wide spectrum of propionate- and especially butyrate-producing microbes, such as the next-generation probiotics Faecalibacterium prausnitzii and Akkermansia muciniphila, were donor-dependently enhanced in the distal colon, which is of specific interest as many colonic diseases originate in the distal part of the colon. For the first time beneficial effects of lactulose on the microbiota as well as metabolic activity could be demonstrated over the entire colon in vitro.

Published in	International Journal of Nutrition and Food Sciences (Volume 8, Issue 1)
DOI	10.11648/j.ijnfs.20190801.12
Page(s)	10-22
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2019. Published by Science Publishing Group

Keywords

Bifidobacteria, Faecalibacterium, Akkermansia, SHIME®, Microbial

References

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Cindy Duysburgh, Pieter Van Den Abbeele, Melanie Bothe, John Stover, Angelika Kuchinka-Koch, et al. (2019). Lactulose Crystals Beneficially Affect Community Composition Along Entire Human Colon in Vitro, Resulting in Donor-Dependent Prebiotic Effects at Metabolic Level. International Journal of Nutrition and Food Sciences, 8(1), 10-22. https://doi.org/10.11648/j.ijnfs.20190801.12

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ACS Style

Cindy Duysburgh; Pieter Van Den Abbeele; Melanie Bothe; John Stover; Angelika Kuchinka-Koch, et al. Lactulose Crystals Beneficially Affect Community Composition Along Entire Human Colon in Vitro, Resulting in Donor-Dependent Prebiotic Effects at Metabolic Level. Int. J. Nutr. Food Sci. 2019, 8(1), 10-22. doi: 10.11648/j.ijnfs.20190801.12

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AMA Style

Cindy Duysburgh, Pieter Van Den Abbeele, Melanie Bothe, John Stover, Angelika Kuchinka-Koch, et al. Lactulose Crystals Beneficially Affect Community Composition Along Entire Human Colon in Vitro, Resulting in Donor-Dependent Prebiotic Effects at Metabolic Level. Int J Nutr Food Sci. 2019;8(1):10-22. doi: 10.11648/j.ijnfs.20190801.12

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@article{10.11648/j.ijnfs.20190801.12,
  author = {Cindy Duysburgh and Pieter Van Den Abbeele and Melanie Bothe and John Stover and Angelika Kuchinka-Koch and Susann Schwejda-Guettes and Massimo Marzorati},
  title = {Lactulose Crystals Beneficially Affect Community Composition Along Entire Human Colon in Vitro, Resulting in Donor-Dependent Prebiotic Effects at Metabolic Level},
  journal = {International Journal of Nutrition and Food Sciences},
  volume = {8},
  number = {1},
  pages = {10-22},
  doi = {10.11648/j.ijnfs.20190801.12},
  url = {https://doi.org/10.11648/j.ijnfs.20190801.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijnfs.20190801.12},
  abstract = {A validated in vitro gut model (i.e. SHIME®) was used to assess the effect of repeated daily administration of lactulose on microbial metabolic activity and community composition in different colonic areas, with the focus on inter-individual differences among three human subjects. An initial rise in acetate and lactate levels was observed in the proximal colon after lactulose administration, which could be linked to an overall strong bifidogenic effect as well as higher Lactobacilli levels in donors 2 and 3. Particularly two operational taxonomic units (OTUs) related to Bifidobacterium adolescentis and Bifidobacterium longum increased with lactulose addition. The enhanced acetate and lactate production subsequently stimulated microbial species involved in cross-feeding interactions, resulting in the donor-dependent production of propionate and/or butyrate. Additionally, a reduction in markers of proteolytic fermentation was detected upon lactulose supplementation. A wide spectrum of propionate- and especially butyrate-producing microbes, such as the next-generation probiotics Faecalibacterium prausnitzii and Akkermansia muciniphila, were donor-dependently enhanced in the distal colon, which is of specific interest as many colonic diseases originate in the distal part of the colon. For the first time beneficial effects of lactulose on the microbiota as well as metabolic activity could be demonstrated over the entire colon in vitro.},
 year = {2019}
}

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TY  - JOUR
T1  - Lactulose Crystals Beneficially Affect Community Composition Along Entire Human Colon in Vitro, Resulting in Donor-Dependent Prebiotic Effects at Metabolic Level
AU  - Cindy Duysburgh
AU  - Pieter Van Den Abbeele
AU  - Melanie Bothe
AU  - John Stover
AU  - Angelika Kuchinka-Koch
AU  - Susann Schwejda-Guettes
AU  - Massimo Marzorati
Y1  - 2019/04/29
PY  - 2019
N1  - https://doi.org/10.11648/j.ijnfs.20190801.12
DO  - 10.11648/j.ijnfs.20190801.12
T2  - International Journal of Nutrition and Food Sciences
JF  - International Journal of Nutrition and Food Sciences
JO  - International Journal of Nutrition and Food Sciences
SP  - 10
EP  - 22
PB  - Science Publishing Group
SN  - 2327-2716
UR  - https://doi.org/10.11648/j.ijnfs.20190801.12
AB  - A validated in vitro gut model (i.e. SHIME®) was used to assess the effect of repeated daily administration of lactulose on microbial metabolic activity and community composition in different colonic areas, with the focus on inter-individual differences among three human subjects. An initial rise in acetate and lactate levels was observed in the proximal colon after lactulose administration, which could be linked to an overall strong bifidogenic effect as well as higher Lactobacilli levels in donors 2 and 3. Particularly two operational taxonomic units (OTUs) related to Bifidobacterium adolescentis and Bifidobacterium longum increased with lactulose addition. The enhanced acetate and lactate production subsequently stimulated microbial species involved in cross-feeding interactions, resulting in the donor-dependent production of propionate and/or butyrate. Additionally, a reduction in markers of proteolytic fermentation was detected upon lactulose supplementation. A wide spectrum of propionate- and especially butyrate-producing microbes, such as the next-generation probiotics Faecalibacterium prausnitzii and Akkermansia muciniphila, were donor-dependently enhanced in the distal colon, which is of specific interest as many colonic diseases originate in the distal part of the colon. For the first time beneficial effects of lactulose on the microbiota as well as metabolic activity could be demonstrated over the entire colon in vitro.
VL  - 8
IS  - 1
ER  -

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Author Information

Cindy Duysburgh

Pro Digest Bvba, Ghent, Belgium
Pieter Van Den Abbeele

Pro Digest Bvba, Ghent, Belgium
Melanie Bothe

Fresenius-Kabi Deutschland GmbH, Bad Homburg, Germany
John Stover

Fresenius-Kabi Deutschland GmbH, Oberursel, Germany
Angelika Kuchinka-Koch

Fresenius-Kabi Austria GmbH, Linz, Austria
Susann Schwejda-Guettes

Fresenius-Kabi Deutschland GmbH, Oberursel, Germany
Massimo Marzorati

Pro Digest Bvba, Ghent, Belgium

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Plain Text BibTeX RIS

APA Style

Cindy Duysburgh, Pieter Van Den Abbeele, Melanie Bothe, John Stover, Angelika Kuchinka-Koch, et al. (2019). Lactulose Crystals Beneficially Affect Community Composition Along Entire Human Colon in Vitro, Resulting in Donor-Dependent Prebiotic Effects at Metabolic Level. International Journal of Nutrition and Food Sciences, 8(1), 10-22. https://doi.org/10.11648/j.ijnfs.20190801.12

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ACS Style

Cindy Duysburgh; Pieter Van Den Abbeele; Melanie Bothe; John Stover; Angelika Kuchinka-Koch, et al. Lactulose Crystals Beneficially Affect Community Composition Along Entire Human Colon in Vitro, Resulting in Donor-Dependent Prebiotic Effects at Metabolic Level. Int. J. Nutr. Food Sci. 2019, 8(1), 10-22. doi: 10.11648/j.ijnfs.20190801.12

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AMA Style

Cindy Duysburgh, Pieter Van Den Abbeele, Melanie Bothe, John Stover, Angelika Kuchinka-Koch, et al. Lactulose Crystals Beneficially Affect Community Composition Along Entire Human Colon in Vitro, Resulting in Donor-Dependent Prebiotic Effects at Metabolic Level. Int J Nutr Food Sci. 2019;8(1):10-22. doi: 10.11648/j.ijnfs.20190801.12

Copy | Download

@article{10.11648/j.ijnfs.20190801.12,
  author = {Cindy Duysburgh and Pieter Van Den Abbeele and Melanie Bothe and John Stover and Angelika Kuchinka-Koch and Susann Schwejda-Guettes and Massimo Marzorati},
  title = {Lactulose Crystals Beneficially Affect Community Composition Along Entire Human Colon in Vitro, Resulting in Donor-Dependent Prebiotic Effects at Metabolic Level},
  journal = {International Journal of Nutrition and Food Sciences},
  volume = {8},
  number = {1},
  pages = {10-22},
  doi = {10.11648/j.ijnfs.20190801.12},
  url = {https://doi.org/10.11648/j.ijnfs.20190801.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijnfs.20190801.12},
  abstract = {A validated in vitro gut model (i.e. SHIME®) was used to assess the effect of repeated daily administration of lactulose on microbial metabolic activity and community composition in different colonic areas, with the focus on inter-individual differences among three human subjects. An initial rise in acetate and lactate levels was observed in the proximal colon after lactulose administration, which could be linked to an overall strong bifidogenic effect as well as higher Lactobacilli levels in donors 2 and 3. Particularly two operational taxonomic units (OTUs) related to Bifidobacterium adolescentis and Bifidobacterium longum increased with lactulose addition. The enhanced acetate and lactate production subsequently stimulated microbial species involved in cross-feeding interactions, resulting in the donor-dependent production of propionate and/or butyrate. Additionally, a reduction in markers of proteolytic fermentation was detected upon lactulose supplementation. A wide spectrum of propionate- and especially butyrate-producing microbes, such as the next-generation probiotics Faecalibacterium prausnitzii and Akkermansia muciniphila, were donor-dependently enhanced in the distal colon, which is of specific interest as many colonic diseases originate in the distal part of the colon. For the first time beneficial effects of lactulose on the microbiota as well as metabolic activity could be demonstrated over the entire colon in vitro.},
 year = {2019}
}

Copy | Download

TY  - JOUR
T1  - Lactulose Crystals Beneficially Affect Community Composition Along Entire Human Colon in Vitro, Resulting in Donor-Dependent Prebiotic Effects at Metabolic Level
AU  - Cindy Duysburgh
AU  - Pieter Van Den Abbeele
AU  - Melanie Bothe
AU  - John Stover
AU  - Angelika Kuchinka-Koch
AU  - Susann Schwejda-Guettes
AU  - Massimo Marzorati
Y1  - 2019/04/29
PY  - 2019
N1  - https://doi.org/10.11648/j.ijnfs.20190801.12
DO  - 10.11648/j.ijnfs.20190801.12
T2  - International Journal of Nutrition and Food Sciences
JF  - International Journal of Nutrition and Food Sciences
JO  - International Journal of Nutrition and Food Sciences
SP  - 10
EP  - 22
PB  - Science Publishing Group
SN  - 2327-2716
UR  - https://doi.org/10.11648/j.ijnfs.20190801.12
AB  - A validated in vitro gut model (i.e. SHIME®) was used to assess the effect of repeated daily administration of lactulose on microbial metabolic activity and community composition in different colonic areas, with the focus on inter-individual differences among three human subjects. An initial rise in acetate and lactate levels was observed in the proximal colon after lactulose administration, which could be linked to an overall strong bifidogenic effect as well as higher Lactobacilli levels in donors 2 and 3. Particularly two operational taxonomic units (OTUs) related to Bifidobacterium adolescentis and Bifidobacterium longum increased with lactulose addition. The enhanced acetate and lactate production subsequently stimulated microbial species involved in cross-feeding interactions, resulting in the donor-dependent production of propionate and/or butyrate. Additionally, a reduction in markers of proteolytic fermentation was detected upon lactulose supplementation. A wide spectrum of propionate- and especially butyrate-producing microbes, such as the next-generation probiotics Faecalibacterium prausnitzii and Akkermansia muciniphila, were donor-dependently enhanced in the distal colon, which is of specific interest as many colonic diseases originate in the distal part of the colon. For the first time beneficial effects of lactulose on the microbiota as well as metabolic activity could be demonstrated over the entire colon in vitro.
VL  - 8
IS  - 1
ER  -

Copy | Download