Estimation of L-lysine Requirement by Indicator Amino Acid Oxidation Method Using Random Effects Model

Kohsuke Hayamizu; Keisuke Matsumoto; Nobuo Izumo; Makoto Nakano

doi:doi:10.11648/j.ijnfs.20200902.14

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Estimation of L-lysine Requirement by Indicator Amino Acid Oxidation Method Using Random Effects Model

Kohsuke Hayamizu, Keisuke Matsumoto, Nobuo Izumo, Makoto Nakano

Published in International Journal of Nutrition and Food Sciences (Volume 9, Issue 2)

Received: 7 April 2020 Accepted: 22 April 2020 Published: 14 May 2020

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Abstract

In recent years, it has been suggested that the method for determining the requirements for indispensable (essential) amino acids be changed from the nitrogen balance method to the tracer methods. In particular, the indicator amino acid oxidation (IAAO) method has been implemented. Although the requirements for indispensable amino acids have been reported in several independent trials, no reported study has statistically integrated these data. In addition, the requirement as obtained from the IAAO method reported to date is the estimated average requirement (EAR), which will be met nutrient requirements in 50% of population only; thus, the risk of deficiency cannot be ruled out from a nutritional perspective. In this study, we statistically synthesized the data of multiple required amounts of lysine obtained by the IAAO method and attempted to accurately estimate the EAR. Further, we estimated the recommended dietary allowance (RDA) value from the obtained estimated EAR value. Analysis using a random effects model estimated that the EAR of lysine in healthy adults was approximately 37 mg/kg (95% CI: 31.2–42.5). In addition, the RDA was estimated to be about 46 mg/kg. These values are higher than the previously reported value of 30 mg/kg.

Published in	International Journal of Nutrition and Food Sciences (Volume 9, Issue 2)
DOI	10.11648/j.ijnfs.20200902.14
Page(s)	63-68
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2020. Published by Science Publishing Group

Keywords

L-lysine, Indicator Amino Acid Oxidation, Estimated Average Requirement, Recommended Dietary Allowance

References

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[5]	G. A. Zello, P. B. Pencharz, and R. O. Ball, “Dietary lysine requirement of young adult males determined by oxidation of L-[1-¹³C] phenylalanine,” Am. J. Physiol., vol. 264, pp. E677–685, 1993.
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[7]	W. Kriengsinyos, L. J. Wykes, R. O. Ball, and P. B. Pencharz, “Oral and intravenous tracer protocols of the indicator amino acid oxidation method provide the same estimate of the lysine requirement in healthy men,” J. Nutr., vol. 132, pp. 2251–2257, 2002.
[8]	W. Kriengsinyos, L. J. Wykes, L. A. Goonewardene, R. O. Ball, and P. B. Pencharz, “Phase of menstrual cycle affects lysine requirement in healthy women,” Am. J. Physiol. Endocrinol. Metab., vol. 287, pp. E489–496, 2004.
[9]	M. Payne, T. Stephens, K. Lim, R. O. Ball, P. B. Pencharz, and R. Elango, “Lysine requirements of healthy pregnant women are higher during late stages of gestation compared to early gestation,” J. Nutr., vol. 148, pp. 94–99, 2018.
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[12]	A. V. Kurpad, T. Raj, A. El-Khoury, L. Beaumier, R. Kuriyan, A. Srivatsa, S. Borgonha, A. Selvaraj, M. M. Regan, and V. R. Young, “Lysine requirements of healthy adult Indian subjects, measured by an indicator amino acid balance technique,” Am. J. Clin. Nutr., vol. 73, pp. 900–907, 2001.
[13]	K. I. Kim, I. McMillan, and H. S. Bayley, “Determination of amino acid requirements of young pigs using an indicator amino acid,” Br. J. Nutr., 50, 369–382, 1983.
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APA Style

Kohsuke Hayamizu, Keisuke Matsumoto, Nobuo Izumo, Makoto Nakano. (2020). Estimation of L-lysine Requirement by Indicator Amino Acid Oxidation Method Using Random Effects Model. International Journal of Nutrition and Food Sciences, 9(2), 63-68. https://doi.org/10.11648/j.ijnfs.20200902.14

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ACS Style

Kohsuke Hayamizu; Keisuke Matsumoto; Nobuo Izumo; Makoto Nakano. Estimation of L-lysine Requirement by Indicator Amino Acid Oxidation Method Using Random Effects Model. Int. J. Nutr. Food Sci. 2020, 9(2), 63-68. doi: 10.11648/j.ijnfs.20200902.14

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AMA Style

Kohsuke Hayamizu, Keisuke Matsumoto, Nobuo Izumo, Makoto Nakano. Estimation of L-lysine Requirement by Indicator Amino Acid Oxidation Method Using Random Effects Model. Int J Nutr Food Sci. 2020;9(2):63-68. doi: 10.11648/j.ijnfs.20200902.14

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@article{10.11648/j.ijnfs.20200902.14,
  author = {Kohsuke Hayamizu and Keisuke Matsumoto and Nobuo Izumo and Makoto Nakano},
  title = {Estimation of L-lysine Requirement by Indicator Amino Acid Oxidation Method Using Random Effects Model},
  journal = {International Journal of Nutrition and Food Sciences},
  volume = {9},
  number = {2},
  pages = {63-68},
  doi = {10.11648/j.ijnfs.20200902.14},
  url = {https://doi.org/10.11648/j.ijnfs.20200902.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijnfs.20200902.14},
  abstract = {In recent years, it has been suggested that the method for determining the requirements for indispensable (essential) amino acids be changed from the nitrogen balance method to the tracer methods. In particular, the indicator amino acid oxidation (IAAO) method has been implemented. Although the requirements for indispensable amino acids have been reported in several independent trials, no reported study has statistically integrated these data. In addition, the requirement as obtained from the IAAO method reported to date is the estimated average requirement (EAR), which will be met nutrient requirements in 50% of population only; thus, the risk of deficiency cannot be ruled out from a nutritional perspective. In this study, we statistically synthesized the data of multiple required amounts of lysine obtained by the IAAO method and attempted to accurately estimate the EAR. Further, we estimated the recommended dietary allowance (RDA) value from the obtained estimated EAR value. Analysis using a random effects model estimated that the EAR of lysine in healthy adults was approximately 37 mg/kg (95% CI: 31.2–42.5). In addition, the RDA was estimated to be about 46 mg/kg. These values are higher than the previously reported value of 30 mg/kg.},
 year = {2020}
}

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TY  - JOUR
T1  - Estimation of L-lysine Requirement by Indicator Amino Acid Oxidation Method Using Random Effects Model
AU  - Kohsuke Hayamizu
AU  - Keisuke Matsumoto
AU  - Nobuo Izumo
AU  - Makoto Nakano
Y1  - 2020/05/14
PY  - 2020
N1  - https://doi.org/10.11648/j.ijnfs.20200902.14
DO  - 10.11648/j.ijnfs.20200902.14
T2  - International Journal of Nutrition and Food Sciences
JF  - International Journal of Nutrition and Food Sciences
JO  - International Journal of Nutrition and Food Sciences
SP  - 63
EP  - 68
PB  - Science Publishing Group
SN  - 2327-2716
UR  - https://doi.org/10.11648/j.ijnfs.20200902.14
AB  - In recent years, it has been suggested that the method for determining the requirements for indispensable (essential) amino acids be changed from the nitrogen balance method to the tracer methods. In particular, the indicator amino acid oxidation (IAAO) method has been implemented. Although the requirements for indispensable amino acids have been reported in several independent trials, no reported study has statistically integrated these data. In addition, the requirement as obtained from the IAAO method reported to date is the estimated average requirement (EAR), which will be met nutrient requirements in 50% of population only; thus, the risk of deficiency cannot be ruled out from a nutritional perspective. In this study, we statistically synthesized the data of multiple required amounts of lysine obtained by the IAAO method and attempted to accurately estimate the EAR. Further, we estimated the recommended dietary allowance (RDA) value from the obtained estimated EAR value. Analysis using a random effects model estimated that the EAR of lysine in healthy adults was approximately 37 mg/kg (95% CI: 31.2–42.5). In addition, the RDA was estimated to be about 46 mg/kg. These values are higher than the previously reported value of 30 mg/kg.
VL  - 9
IS  - 2
ER  -

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Author Information

Kohsuke Hayamizu

Department of Pharmacy, Yokohama University of Pharmacy, Yokohama, Japan
Keisuke Matsumoto

Department of Pharmacy, Yokohama University of Pharmacy, Yokohama, Japan
Nobuo Izumo

Department of Pharmacy, Yokohama University of Pharmacy, Yokohama, Japan
Makoto Nakano

Department of Pharmacy, Yokohama University of Pharmacy, Yokohama, Japan

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APA Style

Kohsuke Hayamizu, Keisuke Matsumoto, Nobuo Izumo, Makoto Nakano. (2020). Estimation of L-lysine Requirement by Indicator Amino Acid Oxidation Method Using Random Effects Model. International Journal of Nutrition and Food Sciences, 9(2), 63-68. https://doi.org/10.11648/j.ijnfs.20200902.14

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ACS Style

Kohsuke Hayamizu; Keisuke Matsumoto; Nobuo Izumo; Makoto Nakano. Estimation of L-lysine Requirement by Indicator Amino Acid Oxidation Method Using Random Effects Model. Int. J. Nutr. Food Sci. 2020, 9(2), 63-68. doi: 10.11648/j.ijnfs.20200902.14

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AMA Style

Kohsuke Hayamizu, Keisuke Matsumoto, Nobuo Izumo, Makoto Nakano. Estimation of L-lysine Requirement by Indicator Amino Acid Oxidation Method Using Random Effects Model. Int J Nutr Food Sci. 2020;9(2):63-68. doi: 10.11648/j.ijnfs.20200902.14

Copy | Download

@article{10.11648/j.ijnfs.20200902.14,
  author = {Kohsuke Hayamizu and Keisuke Matsumoto and Nobuo Izumo and Makoto Nakano},
  title = {Estimation of L-lysine Requirement by Indicator Amino Acid Oxidation Method Using Random Effects Model},
  journal = {International Journal of Nutrition and Food Sciences},
  volume = {9},
  number = {2},
  pages = {63-68},
  doi = {10.11648/j.ijnfs.20200902.14},
  url = {https://doi.org/10.11648/j.ijnfs.20200902.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijnfs.20200902.14},
  abstract = {In recent years, it has been suggested that the method for determining the requirements for indispensable (essential) amino acids be changed from the nitrogen balance method to the tracer methods. In particular, the indicator amino acid oxidation (IAAO) method has been implemented. Although the requirements for indispensable amino acids have been reported in several independent trials, no reported study has statistically integrated these data. In addition, the requirement as obtained from the IAAO method reported to date is the estimated average requirement (EAR), which will be met nutrient requirements in 50% of population only; thus, the risk of deficiency cannot be ruled out from a nutritional perspective. In this study, we statistically synthesized the data of multiple required amounts of lysine obtained by the IAAO method and attempted to accurately estimate the EAR. Further, we estimated the recommended dietary allowance (RDA) value from the obtained estimated EAR value. Analysis using a random effects model estimated that the EAR of lysine in healthy adults was approximately 37 mg/kg (95% CI: 31.2–42.5). In addition, the RDA was estimated to be about 46 mg/kg. These values are higher than the previously reported value of 30 mg/kg.},
 year = {2020}
}

Copy | Download

TY  - JOUR
T1  - Estimation of L-lysine Requirement by Indicator Amino Acid Oxidation Method Using Random Effects Model
AU  - Kohsuke Hayamizu
AU  - Keisuke Matsumoto
AU  - Nobuo Izumo
AU  - Makoto Nakano
Y1  - 2020/05/14
PY  - 2020
N1  - https://doi.org/10.11648/j.ijnfs.20200902.14
DO  - 10.11648/j.ijnfs.20200902.14
T2  - International Journal of Nutrition and Food Sciences
JF  - International Journal of Nutrition and Food Sciences
JO  - International Journal of Nutrition and Food Sciences
SP  - 63
EP  - 68
PB  - Science Publishing Group
SN  - 2327-2716
UR  - https://doi.org/10.11648/j.ijnfs.20200902.14
AB  - In recent years, it has been suggested that the method for determining the requirements for indispensable (essential) amino acids be changed from the nitrogen balance method to the tracer methods. In particular, the indicator amino acid oxidation (IAAO) method has been implemented. Although the requirements for indispensable amino acids have been reported in several independent trials, no reported study has statistically integrated these data. In addition, the requirement as obtained from the IAAO method reported to date is the estimated average requirement (EAR), which will be met nutrient requirements in 50% of population only; thus, the risk of deficiency cannot be ruled out from a nutritional perspective. In this study, we statistically synthesized the data of multiple required amounts of lysine obtained by the IAAO method and attempted to accurately estimate the EAR. Further, we estimated the recommended dietary allowance (RDA) value from the obtained estimated EAR value. Analysis using a random effects model estimated that the EAR of lysine in healthy adults was approximately 37 mg/kg (95% CI: 31.2–42.5). In addition, the RDA was estimated to be about 46 mg/kg. These values are higher than the previously reported value of 30 mg/kg.
VL  - 9
IS  - 2
ER  -

Copy | Download