Acrylamide, exists in carbohydrate-rich food heated at high temperatures, is a probable carcinogen to humans. Excessive alcohol intake can also lead to a variety of pathological changes in the body. Acrylamide in the foods and alcohol in the drinks are unavoidable. And studies have demonstrated that combination of the two substances which are taken into the body via diet may cause adverse effects in the cells, even induce impairments on testicular spermatogenesis in male offsprings. Both acrylamide and alcohol are mainly metabolized in the liver, where cytochrome P450 2E1 (CYP2E1) acts as the common metabolic enzyme of the two xenobiotics. This study aimed to explore the effects of acrylamide and alcohol combination on rat hepatoma BRL cells proliferation and the probable mechanisms. MTT, western blotting, EdU fluorescence staining, flow cytometry and PCR were used. Results showed that combination of acrylamide and alcohol at low doses promoted BRL cells proliferation through CYP2E1/Akt/ NF-κB/cyclinB1/cyclinD1 activation and ROS production. The combined effects of acrylamide and alcohol largely depended on ROS level. Furthermore, acrylamide and alcohol could synergistically induce miR-21, which was related to the progress of liver regeneration. These data showed that combination of acrylamide and alcohol at low doses promoted BRL cells proliferation through inducing ROS production, which indicated that intake of fried starch food with small dose of alcohol might have positive effects on liver regeneration.
| Published in | International Journal of Nutrition and Food Sciences (Volume 9, Issue 3) |
| DOI | 10.11648/j.ijnfs.20200903.12 |
| Page(s) | 78-85 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2020. Published by Science Publishing Group |
Acrylamide, Alcohol, Proliferation, ROS, miR-21
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APA Style
Xiaoyun Shan, Pengqi Wang, Qing Feng. (2020). Rat Hepatoma BRL Cells Proliferation by Acrylamide and Alcohol Combination. International Journal of Nutrition and Food Sciences, 9(3), 78-85. https://doi.org/10.11648/j.ijnfs.20200903.12
ACS Style
Xiaoyun Shan; Pengqi Wang; Qing Feng. Rat Hepatoma BRL Cells Proliferation by Acrylamide and Alcohol Combination. Int. J. Nutr. Food Sci. 2020, 9(3), 78-85. doi: 10.11648/j.ijnfs.20200903.12
AMA Style
Xiaoyun Shan, Pengqi Wang, Qing Feng. Rat Hepatoma BRL Cells Proliferation by Acrylamide and Alcohol Combination. Int J Nutr Food Sci. 2020;9(3):78-85. doi: 10.11648/j.ijnfs.20200903.12
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Download@article{10.11648/j.ijnfs.20200903.12,
author = {Xiaoyun Shan and Pengqi Wang and Qing Feng},
title = {Rat Hepatoma BRL Cells Proliferation by Acrylamide and Alcohol Combination},
journal = {International Journal of Nutrition and Food Sciences},
volume = {9},
number = {3},
pages = {78-85},
doi = {10.11648/j.ijnfs.20200903.12},
url = {https://doi.org/10.11648/j.ijnfs.20200903.12},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijnfs.20200903.12},
abstract = {Acrylamide, exists in carbohydrate-rich food heated at high temperatures, is a probable carcinogen to humans. Excessive alcohol intake can also lead to a variety of pathological changes in the body. Acrylamide in the foods and alcohol in the drinks are unavoidable. And studies have demonstrated that combination of the two substances which are taken into the body via diet may cause adverse effects in the cells, even induce impairments on testicular spermatogenesis in male offsprings. Both acrylamide and alcohol are mainly metabolized in the liver, where cytochrome P450 2E1 (CYP2E1) acts as the common metabolic enzyme of the two xenobiotics. This study aimed to explore the effects of acrylamide and alcohol combination on rat hepatoma BRL cells proliferation and the probable mechanisms. MTT, western blotting, EdU fluorescence staining, flow cytometry and PCR were used. Results showed that combination of acrylamide and alcohol at low doses promoted BRL cells proliferation through CYP2E1/Akt/ NF-κB/cyclinB1/cyclinD1 activation and ROS production. The combined effects of acrylamide and alcohol largely depended on ROS level. Furthermore, acrylamide and alcohol could synergistically induce miR-21, which was related to the progress of liver regeneration. These data showed that combination of acrylamide and alcohol at low doses promoted BRL cells proliferation through inducing ROS production, which indicated that intake of fried starch food with small dose of alcohol might have positive effects on liver regeneration.},
year = {2020}
}
TY - JOUR T1 - Rat Hepatoma BRL Cells Proliferation by Acrylamide and Alcohol Combination AU - Xiaoyun Shan AU - Pengqi Wang AU - Qing Feng Y1 - 2020/06/23 PY - 2020 N1 - https://doi.org/10.11648/j.ijnfs.20200903.12 DO - 10.11648/j.ijnfs.20200903.12 T2 - International Journal of Nutrition and Food Sciences JF - International Journal of Nutrition and Food Sciences JO - International Journal of Nutrition and Food Sciences SP - 78 EP - 85 PB - Science Publishing Group SN - 2327-2716 UR - https://doi.org/10.11648/j.ijnfs.20200903.12 AB - Acrylamide, exists in carbohydrate-rich food heated at high temperatures, is a probable carcinogen to humans. Excessive alcohol intake can also lead to a variety of pathological changes in the body. Acrylamide in the foods and alcohol in the drinks are unavoidable. And studies have demonstrated that combination of the two substances which are taken into the body via diet may cause adverse effects in the cells, even induce impairments on testicular spermatogenesis in male offsprings. Both acrylamide and alcohol are mainly metabolized in the liver, where cytochrome P450 2E1 (CYP2E1) acts as the common metabolic enzyme of the two xenobiotics. This study aimed to explore the effects of acrylamide and alcohol combination on rat hepatoma BRL cells proliferation and the probable mechanisms. MTT, western blotting, EdU fluorescence staining, flow cytometry and PCR were used. Results showed that combination of acrylamide and alcohol at low doses promoted BRL cells proliferation through CYP2E1/Akt/ NF-κB/cyclinB1/cyclinD1 activation and ROS production. The combined effects of acrylamide and alcohol largely depended on ROS level. Furthermore, acrylamide and alcohol could synergistically induce miR-21, which was related to the progress of liver regeneration. These data showed that combination of acrylamide and alcohol at low doses promoted BRL cells proliferation through inducing ROS production, which indicated that intake of fried starch food with small dose of alcohol might have positive effects on liver regeneration. VL - 9 IS - 3 ER -
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