The Potential Roles of CDO1 and JAM3 Methylation in the Occurrence of Nasopharyngeal Carcinoma

Song Zhang; Chong Sai; Yi-Ling Lin; Jia-Hui Tang; Hong-Yan Yuan

doi:doi:10.11648/j.ijo.20230902.12

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The Potential Roles of CDO1 and JAM3 Methylation in the Occurrence of Nasopharyngeal Carcinoma

Song Zhang^*, Chong Sai, Yi-Ling Lin, Jia-Hui Tang, Hong-Yan Yuan

Published in International Journal of Otorhinolaryngology (Volume 9, Issue 2)

Received: 22 June 2023 Accepted: 10 July 2023 Published: 20 July 2023

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Abstract

Background: Nasopharyngeal carcinoma (NPC) is a highly malignant tumor derived from nasopharyngeal epithelial tissue. Due to their strong capacity for hidden lesions and unobvious early symptoms, Given the critical problem for clinical diagnosis, NPC has a high rate of misdiagnosed and missed, which seriously threatens human health. The underlying mechanism of the occurrence and development of nasopharyngeal carcinoma is still not elucidated. In recent years, increasing attention has been paid to the study of some tumor-related genes, especially the epigenetic regulation of genes. Objective: To investigate the role of CDO1 and JAM3 gene methylation in the occurrence of nasopharyngeal carcinoma. Methods: Methylation-specific PCR and pyrosequencing were used to detect the difference in methylation rates of CDO1 and JAM3 in nasopharyngeal carcinoma and nasopharyngeal chronic inflammation tissues. The abundance of protein expression and mRNA for CDO1 and JAM3 were detected by fluorescence quantitative PCR and Western Blot in nasopharyngeal carcinoma and chronic nasopharyngitis tissues. Results: Pyrosequencing indicated that the methylation rates of CDO1 and JAM3 in nasopharyngeal carcinoma tissues were remarkably increased, compared with nasopharyngeal chronic inflammation tissues. Furthermore, the qPCR and Western Blot suggested that the expression of CDO1 and JAM3 in nasopharyngeal carcinoma tissues was significantly lower than that in nasopharyngeal chronic inflammation tissues. Conclusion: The methylation of the CpG island in the promoter region of CDO1 and JAM3 genes leads to a decrease in their gene expression, which may be related to the occurrence of nasopharyngeal carcinoma.

Published in	International Journal of Otorhinolaryngology (Volume 9, Issue 2)
DOI	10.11648/j.ijo.20230902.12
Page(s)	20-25
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2023. Published by Science Publishing Group

Keywords

Nasopharyngeal Carcinoma, Cdo1, Jam3, Methylation

References

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[4]	Brait M, Ling S, Nagpal J K, Chang X et al. Cysteine Dioxygenase1 Is a Tumor Suppressor Gene Silenced by Promoter Methylation in Multiple Human Cancers. PLOS ONE (2012), 7 (9), e44951.
[5]	Zhou D, Tang W, Zhang Y, An HX. JAM3 functions as a novel tumor suppressor and is inactivated by DNA methylation in colorectal cancer. Cancer Management and Research 2019: 11 2457–2470.
[6]	Kojima K, Nakamura T, Ohbu M, Katoh H, Ooizumi Y, Igarashi K, Ishii S. et al. Cysteine dioxygenase type 1 (CDO1) gene promoter methylation during the adenomacarcinoma sequence in colorectal cancer [J]. PLoS ONE. 2018; 13 (5): e0194785.
[7]	Igarashi K, Yamashita K, Katoh H, Kojima K, Ooizumi Y, Nishizawa N, et al. Prognostic significance of promoter DNA hypermethylation of the cysteine dioxygenase 1 (CDO1) gene in primary gallbladder cancer and gallbladder disease. PLoS One. 2017; 12 (11): e0188178.
[8]	Ushiku H, Yamashita K, Katoh H, Ema A, Minatani N, Kikuchi M, et al. Promoter DNA methylation of CDO1 gene and its clinical significance in esophageal squamous cell carcinoma. Dis Esophagus. 2017; 30 (2): 1–9.
[9]	Yang Y, Fan YC, Gao S, Dou CY, et al. methylated cysteine dioxygenase-1 gene promoter in the serum is a potential biomarker for hepatitis B virus-related hepatocellular carcinoma. Tohoku J Exp Med, 2014; 232 (3): 187-94.
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[12]	Kong WJ, Zhang S, Guo C, Zhang S, Wang Y, Zhang D, et al. Methylation- associated silencing of death- protein kinase gene in laryngeal squamous cell cancer. Laryngoscope 2005; 115: 1395-1401.
[13]	Kong WJ, Zhang S, Guo CK, Wang YJ, Chen X, Zhang SL, Zhang D, Liu Z and Kong W. Effect of methylation-associated silencing of the deathassociated protein kinase gene on nasopharyngeal carcinoma. Anti-Cancer Drugs, 2006, 17 (3): 251-259.
[14]	Zhang S, Li S and Gao JL. Promoter methylation status of the tumor suppressor gene SOX11 is associated with cell growth and invasion in nasopharyngeal carcinoma. Cancer Cell International, 2013, 13: 109.

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APA Style

Zhang, S., Sai, C., Lin, Y., Tang, J., Yuan, H. (2023). The Potential Roles of CDO1 and JAM3 Methylation in the Occurrence of Nasopharyngeal Carcinoma. International Journal of Otorhinolaryngology, 9(2), 20-25. https://doi.org/10.11648/j.ijo.20230902.12

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ACS Style

Zhang, S.; Sai, C.; Lin, Y.; Tang, J.; Yuan, H. The Potential Roles of CDO1 and JAM3 Methylation in the Occurrence of Nasopharyngeal Carcinoma. Int. J. Otorhinolaryngol. 2023, 9(2), 20-25. doi: 10.11648/j.ijo.20230902.12

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AMA Style

Zhang S, Sai C, Lin Y, Tang J, Yuan H. The Potential Roles of CDO1 and JAM3 Methylation in the Occurrence of Nasopharyngeal Carcinoma. Int J Otorhinolaryngol. 2023;9(2):20-25. doi: 10.11648/j.ijo.20230902.12

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@article{10.11648/j.ijo.20230902.12,
  author = {Song Zhang and Chong Sai and Yi-Ling Lin and Jia-Hui Tang and Hong-Yan Yuan},
  title = {The Potential Roles of CDO1 and JAM3 Methylation in the Occurrence of Nasopharyngeal Carcinoma},
  journal = {International Journal of Otorhinolaryngology},
  volume = {9},
  number = {2},
  pages = {20-25},
  doi = {10.11648/j.ijo.20230902.12},
  url = {https://doi.org/10.11648/j.ijo.20230902.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijo.20230902.12},
  abstract = {Background: Nasopharyngeal carcinoma (NPC) is a highly malignant tumor derived from nasopharyngeal epithelial tissue. Due to their strong capacity for hidden lesions and unobvious early symptoms, Given the critical problem for clinical diagnosis, NPC has a high rate of misdiagnosed and missed, which seriously threatens human health. The underlying mechanism of the occurrence and development of nasopharyngeal carcinoma is still not elucidated. In recent years, increasing attention has been paid to the study of some tumor-related genes, especially the epigenetic regulation of genes. Objective: To investigate the role of CDO1 and JAM3 gene methylation in the occurrence of nasopharyngeal carcinoma. Methods: Methylation-specific PCR and pyrosequencing were used to detect the difference in methylation rates of CDO1 and JAM3 in nasopharyngeal carcinoma and nasopharyngeal chronic inflammation tissues. The abundance of protein expression and mRNA for CDO1 and JAM3 were detected by fluorescence quantitative PCR and Western Blot in nasopharyngeal carcinoma and chronic nasopharyngitis tissues. Results: Pyrosequencing indicated that the methylation rates of CDO1 and JAM3 in nasopharyngeal carcinoma tissues were remarkably increased, compared with nasopharyngeal chronic inflammation tissues. Furthermore, the qPCR and Western Blot suggested that the expression of CDO1 and JAM3 in nasopharyngeal carcinoma tissues was significantly lower than that in nasopharyngeal chronic inflammation tissues. Conclusion: The methylation of the CpG island in the promoter region of CDO1 and JAM3 genes leads to a decrease in their gene expression, which may be related to the occurrence of nasopharyngeal carcinoma.},
 year = {2023}
}

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TY  - JOUR
T1  - The Potential Roles of CDO1 and JAM3 Methylation in the Occurrence of Nasopharyngeal Carcinoma
AU  - Song Zhang
AU  - Chong Sai
AU  - Yi-Ling Lin
AU  - Jia-Hui Tang
AU  - Hong-Yan Yuan
Y1  - 2023/07/20
PY  - 2023
N1  - https://doi.org/10.11648/j.ijo.20230902.12
DO  - 10.11648/j.ijo.20230902.12
T2  - International Journal of Otorhinolaryngology
JF  - International Journal of Otorhinolaryngology
JO  - International Journal of Otorhinolaryngology
SP  - 20
EP  - 25
PB  - Science Publishing Group
SN  - 2472-2413
UR  - https://doi.org/10.11648/j.ijo.20230902.12
AB  - Background: Nasopharyngeal carcinoma (NPC) is a highly malignant tumor derived from nasopharyngeal epithelial tissue. Due to their strong capacity for hidden lesions and unobvious early symptoms, Given the critical problem for clinical diagnosis, NPC has a high rate of misdiagnosed and missed, which seriously threatens human health. The underlying mechanism of the occurrence and development of nasopharyngeal carcinoma is still not elucidated. In recent years, increasing attention has been paid to the study of some tumor-related genes, especially the epigenetic regulation of genes. Objective: To investigate the role of CDO1 and JAM3 gene methylation in the occurrence of nasopharyngeal carcinoma. Methods: Methylation-specific PCR and pyrosequencing were used to detect the difference in methylation rates of CDO1 and JAM3 in nasopharyngeal carcinoma and nasopharyngeal chronic inflammation tissues. The abundance of protein expression and mRNA for CDO1 and JAM3 were detected by fluorescence quantitative PCR and Western Blot in nasopharyngeal carcinoma and chronic nasopharyngitis tissues. Results: Pyrosequencing indicated that the methylation rates of CDO1 and JAM3 in nasopharyngeal carcinoma tissues were remarkably increased, compared with nasopharyngeal chronic inflammation tissues. Furthermore, the qPCR and Western Blot suggested that the expression of CDO1 and JAM3 in nasopharyngeal carcinoma tissues was significantly lower than that in nasopharyngeal chronic inflammation tissues. Conclusion: The methylation of the CpG island in the promoter region of CDO1 and JAM3 genes leads to a decrease in their gene expression, which may be related to the occurrence of nasopharyngeal carcinoma.
VL  - 9
IS  - 2
ER  -

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Author Information

Song Zhang

Department of Otolaryngology, University of Chinese Academy of Science-Shenzhen Hospital, Shenzhen, China

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Chong Sai

Department of Otolaryngology, University of Chinese Academy of Science-Shenzhen Hospital, Shenzhen, China

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Yi-Ling Lin

Department of Otolaryngology, University of Chinese Academy of Science-Shenzhen Hospital, Shenzhen, China

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Jia-Hui Tang

Department of Otolaryngology, University of Chinese Academy of Science-Shenzhen Hospital, Shenzhen, China

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Hong-Yan Yuan

Department of Otolaryngology, University of Chinese Academy of Science-Shenzhen Hospital, Shenzhen, China

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Plain Text BibTeX RIS

APA Style

Zhang, S., Sai, C., Lin, Y., Tang, J., Yuan, H. (2023). The Potential Roles of CDO1 and JAM3 Methylation in the Occurrence of Nasopharyngeal Carcinoma. International Journal of Otorhinolaryngology, 9(2), 20-25. https://doi.org/10.11648/j.ijo.20230902.12

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ACS Style

Zhang, S.; Sai, C.; Lin, Y.; Tang, J.; Yuan, H. The Potential Roles of CDO1 and JAM3 Methylation in the Occurrence of Nasopharyngeal Carcinoma. Int. J. Otorhinolaryngol. 2023, 9(2), 20-25. doi: 10.11648/j.ijo.20230902.12

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AMA Style

Zhang S, Sai C, Lin Y, Tang J, Yuan H. The Potential Roles of CDO1 and JAM3 Methylation in the Occurrence of Nasopharyngeal Carcinoma. Int J Otorhinolaryngol. 2023;9(2):20-25. doi: 10.11648/j.ijo.20230902.12

Copy | Download

@article{10.11648/j.ijo.20230902.12,
  author = {Song Zhang and Chong Sai and Yi-Ling Lin and Jia-Hui Tang and Hong-Yan Yuan},
  title = {The Potential Roles of CDO1 and JAM3 Methylation in the Occurrence of Nasopharyngeal Carcinoma},
  journal = {International Journal of Otorhinolaryngology},
  volume = {9},
  number = {2},
  pages = {20-25},
  doi = {10.11648/j.ijo.20230902.12},
  url = {https://doi.org/10.11648/j.ijo.20230902.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijo.20230902.12},
  abstract = {Background: Nasopharyngeal carcinoma (NPC) is a highly malignant tumor derived from nasopharyngeal epithelial tissue. Due to their strong capacity for hidden lesions and unobvious early symptoms, Given the critical problem for clinical diagnosis, NPC has a high rate of misdiagnosed and missed, which seriously threatens human health. The underlying mechanism of the occurrence and development of nasopharyngeal carcinoma is still not elucidated. In recent years, increasing attention has been paid to the study of some tumor-related genes, especially the epigenetic regulation of genes. Objective: To investigate the role of CDO1 and JAM3 gene methylation in the occurrence of nasopharyngeal carcinoma. Methods: Methylation-specific PCR and pyrosequencing were used to detect the difference in methylation rates of CDO1 and JAM3 in nasopharyngeal carcinoma and nasopharyngeal chronic inflammation tissues. The abundance of protein expression and mRNA for CDO1 and JAM3 were detected by fluorescence quantitative PCR and Western Blot in nasopharyngeal carcinoma and chronic nasopharyngitis tissues. Results: Pyrosequencing indicated that the methylation rates of CDO1 and JAM3 in nasopharyngeal carcinoma tissues were remarkably increased, compared with nasopharyngeal chronic inflammation tissues. Furthermore, the qPCR and Western Blot suggested that the expression of CDO1 and JAM3 in nasopharyngeal carcinoma tissues was significantly lower than that in nasopharyngeal chronic inflammation tissues. Conclusion: The methylation of the CpG island in the promoter region of CDO1 and JAM3 genes leads to a decrease in their gene expression, which may be related to the occurrence of nasopharyngeal carcinoma.},
 year = {2023}
}

Copy | Download

TY  - JOUR
T1  - The Potential Roles of CDO1 and JAM3 Methylation in the Occurrence of Nasopharyngeal Carcinoma
AU  - Song Zhang
AU  - Chong Sai
AU  - Yi-Ling Lin
AU  - Jia-Hui Tang
AU  - Hong-Yan Yuan
Y1  - 2023/07/20
PY  - 2023
N1  - https://doi.org/10.11648/j.ijo.20230902.12
DO  - 10.11648/j.ijo.20230902.12
T2  - International Journal of Otorhinolaryngology
JF  - International Journal of Otorhinolaryngology
JO  - International Journal of Otorhinolaryngology
SP  - 20
EP  - 25
PB  - Science Publishing Group
SN  - 2472-2413
UR  - https://doi.org/10.11648/j.ijo.20230902.12
AB  - Background: Nasopharyngeal carcinoma (NPC) is a highly malignant tumor derived from nasopharyngeal epithelial tissue. Due to their strong capacity for hidden lesions and unobvious early symptoms, Given the critical problem for clinical diagnosis, NPC has a high rate of misdiagnosed and missed, which seriously threatens human health. The underlying mechanism of the occurrence and development of nasopharyngeal carcinoma is still not elucidated. In recent years, increasing attention has been paid to the study of some tumor-related genes, especially the epigenetic regulation of genes. Objective: To investigate the role of CDO1 and JAM3 gene methylation in the occurrence of nasopharyngeal carcinoma. Methods: Methylation-specific PCR and pyrosequencing were used to detect the difference in methylation rates of CDO1 and JAM3 in nasopharyngeal carcinoma and nasopharyngeal chronic inflammation tissues. The abundance of protein expression and mRNA for CDO1 and JAM3 were detected by fluorescence quantitative PCR and Western Blot in nasopharyngeal carcinoma and chronic nasopharyngitis tissues. Results: Pyrosequencing indicated that the methylation rates of CDO1 and JAM3 in nasopharyngeal carcinoma tissues were remarkably increased, compared with nasopharyngeal chronic inflammation tissues. Furthermore, the qPCR and Western Blot suggested that the expression of CDO1 and JAM3 in nasopharyngeal carcinoma tissues was significantly lower than that in nasopharyngeal chronic inflammation tissues. Conclusion: The methylation of the CpG island in the promoter region of CDO1 and JAM3 genes leads to a decrease in their gene expression, which may be related to the occurrence of nasopharyngeal carcinoma.
VL  - 9
IS  - 2
ER  -

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