Mechanism of Interaction Between Cefepime and Papain by Spectroscopic Method

Li Chen; Baosheng Liu; Chundan Wang; Lihua Ma; Hongcai Zhang; Xu Guo

doi:doi:10.11648/j.jmpt.20180401.16

| Peer-Reviewed

Mechanism of Interaction Between Cefepime and Papain by Spectroscopic Method

Li Chen, Baosheng Liu, Chundan Wang, Lihua Ma, Hongcai Zhang, Xu Guo

Published in Journal of Photonic Materials and Technology (Volume 4, Issue 1)

Received: 27 January 2018 Accepted: 16 February 2018 Published: 19 March 2018

Views: Downloads:

Download PDF

Share This Article

Twitter
Linked In
Facebook

Abstract

The reaction mechanism of cefepime (CEF) with papain (PAPA) was investigated by both fluorescence quenching and synchronous fluorescence spectroscopy in different temperature (293, 308 and 318 K). The results demonstrated that the interaction between CEF and PAPA was taking place via static quenching. Thermodynamic parameters revealed that electrostatic interaction played major roles in the interaction. The order of magnitudes of binding constant is 10⁴, and the number of binding site in the system was closed to 1. The binding rates of CEF to PAPA (λ_ex = 280 nm) were 81.46%~95.95% (293K), 80.50%~95.49% (308K) and 79.77%~95.12% (318K). There was a linear relationship between ΔF and the concentrations of CEF in the range of 6.0×10^-6~7.0×10^-5 mol/L. And the correlation coefficient of the linear curve was 0.9973.

Published in	Journal of Photonic Materials and Technology (Volume 4, Issue 1)
DOI	10.11648/j.jmpt.20180401.16
Page(s)	33-38
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2018. Published by Science Publishing Group

Keywords

Cefepime, Papain, Reaction Mechanism, Spectroscopy, Determination

References

[1]	S. V. Burgess, V. H. Mabasa, I. Chow, M. H. Ensom, J. Annals of Pharmacotherapy, 2015, 49, 3.
[2]	A. Meillier, D. Rahimian, J. Oxford Medical Case Reports, 2016, 2016, 6.
[3]	J. Yao, C. Lin, T. Tao, F. Lin, J. Colloids & Surfaces B Biointerfaces, 2013, 101, 101C.
[4]	R. Menard, H. E. Khouri, C. Plouffe, R. Dupras, D. Ripoll, T. Vernet, D. C. Tessier, F. Laliberte, D. Y. Thomas, A. C. Storer, J. Biochemistry, 1990. 29, 28.
[5]	E. V. Mikhalchik, A. V. Ivanova, M. V. Anurov, S. M. Titkova, L. Y. Penkov, Z. F. Kharaeva, L. G. Korkina, J. Bulletin of Experimental Biology & Medicine, 2004, 137 (6):560-562.
[6]	A. B. Khan, J. M. Khan, M. S. Ali, R. H. Khan, Kabir-ud-Din, J. Spectrochimica Acta Part A Molecular & Biomolecular Spectroscopy, 2012, 97, 6.
[7]	Q. Wang, J. He, J. Yan, D. Wu, H. Li, J. Luminescence, 2015, 30, 2.
[8]	Y. Liu, G. Zhang, Y. Liao, Y. Wang, J. Spectrochimica Acta Part A Molecular & Biomolecular Spectroscopy, 2015, 151, 498.
[9]	L. Trnková, I. Boušová, V. Staňková, J. Dršata, J. Journal of Molecular Structure, 2011, 985, 2.
[10]	R. Han, B. Liu, G. Li, Q. Zhang, J. Spectroscopy Letters, 2016, 49, 3.
[11]	G. Wang, Y. Chen, C. Yan, Y. Lu, J. Journal of Luminescence, 2015, 157, 229.
[12]	G. Zhang, X. Chen, J. Guo, J. Wang, J. Journal of Molecular Structure, 2009, 921, 1-3.
[13]	Q. J. Zhang, B. S. Liu, R. Han, G. X. Li, J. Spectroscopy Letters, 2015, 49, 3.

Cite This Article

Plain Text BibTeX RIS

APA Style

Li Chen, Baosheng Liu, Chundan Wang, Lihua Ma, Hongcai Zhang, et al. (2018). Mechanism of Interaction Between Cefepime and Papain by Spectroscopic Method. Journal of Photonic Materials and Technology, 4(1), 33-38. https://doi.org/10.11648/j.jmpt.20180401.16

Copy | Download

ACS Style

Li Chen; Baosheng Liu; Chundan Wang; Lihua Ma; Hongcai Zhang, et al. Mechanism of Interaction Between Cefepime and Papain by Spectroscopic Method. J. Photonic Mater. Technol. 2018, 4(1), 33-38. doi: 10.11648/j.jmpt.20180401.16

Copy | Download

AMA Style

Li Chen, Baosheng Liu, Chundan Wang, Lihua Ma, Hongcai Zhang, et al. Mechanism of Interaction Between Cefepime and Papain by Spectroscopic Method. J Photonic Mater Technol. 2018;4(1):33-38. doi: 10.11648/j.jmpt.20180401.16

Copy | Download

@article{10.11648/j.jmpt.20180401.16,
  author = {Li Chen and Baosheng Liu and Chundan Wang and Lihua Ma and Hongcai Zhang and Xu Guo},
  title = {Mechanism of Interaction Between Cefepime and Papain by Spectroscopic Method},
  journal = {Journal of Photonic Materials and Technology},
  volume = {4},
  number = {1},
  pages = {33-38},
  doi = {10.11648/j.jmpt.20180401.16},
  url = {https://doi.org/10.11648/j.jmpt.20180401.16},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jmpt.20180401.16},
  abstract = {The reaction mechanism of cefepime (CEF) with papain (PAPA) was investigated by both fluorescence quenching and synchronous fluorescence spectroscopy in different temperature (293, 308 and 318 K). The results demonstrated that the interaction between CEF and PAPA was taking place via static quenching. Thermodynamic parameters revealed that electrostatic interaction played major roles in the interaction. The order of magnitudes of binding constant is 104, and the number of binding site in the system was closed to 1. The binding rates of CEF to PAPA (λex = 280 nm) were 81.46%~95.95% (293K), 80.50%~95.49% (308K) and 79.77%~95.12% (318K). There was a linear relationship between ΔF and the concentrations of CEF in the range of 6.0×10-6~7.0×10-5 mol/L. And the correlation coefficient of the linear curve was 0.9973.},
 year = {2018}
}

Copy | Download

TY  - JOUR
T1  - Mechanism of Interaction Between Cefepime and Papain by Spectroscopic Method
AU  - Li Chen
AU  - Baosheng Liu
AU  - Chundan Wang
AU  - Lihua Ma
AU  - Hongcai Zhang
AU  - Xu Guo
Y1  - 2018/03/19
PY  - 2018
N1  - https://doi.org/10.11648/j.jmpt.20180401.16
DO  - 10.11648/j.jmpt.20180401.16
T2  - Journal of Photonic Materials and Technology
JF  - Journal of Photonic Materials and Technology
JO  - Journal of Photonic Materials and Technology
SP  - 33
EP  - 38
PB  - Science Publishing Group
SN  - 2469-8431
UR  - https://doi.org/10.11648/j.jmpt.20180401.16
AB  - The reaction mechanism of cefepime (CEF) with papain (PAPA) was investigated by both fluorescence quenching and synchronous fluorescence spectroscopy in different temperature (293, 308 and 318 K). The results demonstrated that the interaction between CEF and PAPA was taking place via static quenching. Thermodynamic parameters revealed that electrostatic interaction played major roles in the interaction. The order of magnitudes of binding constant is 104, and the number of binding site in the system was closed to 1. The binding rates of CEF to PAPA (λex = 280 nm) were 81.46%~95.95% (293K), 80.50%~95.49% (308K) and 79.77%~95.12% (318K). There was a linear relationship between ΔF and the concentrations of CEF in the range of 6.0×10-6~7.0×10-5 mol/L. And the correlation coefficient of the linear curve was 0.9973.
VL  - 4
IS  - 1
ER  -

Copy | Download

Author Information

Li Chen

College of Chemistry & Environmental Science, Hebei University, Baoding, P. R. China
Baosheng Liu

College of Chemistry & Environmental Science, Hebei University, Baoding, P. R. China
Chundan Wang

College of Chemistry & Environmental Science, Hebei University, Baoding, P. R. China
Lihua Ma

College of Chemistry & Environmental Science, Hebei University, Baoding, P. R. China
Hongcai Zhang

College of Chemistry & Environmental Science, Hebei University, Baoding, P. R. China
Xu Guo

College of Chemistry & Environmental Science, Hebei University, Baoding, P. R. China

Download PDF

Sections

Plain Text BibTeX RIS

APA Style

Li Chen, Baosheng Liu, Chundan Wang, Lihua Ma, Hongcai Zhang, et al. (2018). Mechanism of Interaction Between Cefepime and Papain by Spectroscopic Method. Journal of Photonic Materials and Technology, 4(1), 33-38. https://doi.org/10.11648/j.jmpt.20180401.16

Copy | Download

ACS Style

Li Chen; Baosheng Liu; Chundan Wang; Lihua Ma; Hongcai Zhang, et al. Mechanism of Interaction Between Cefepime and Papain by Spectroscopic Method. J. Photonic Mater. Technol. 2018, 4(1), 33-38. doi: 10.11648/j.jmpt.20180401.16

Copy | Download

AMA Style

Li Chen, Baosheng Liu, Chundan Wang, Lihua Ma, Hongcai Zhang, et al. Mechanism of Interaction Between Cefepime and Papain by Spectroscopic Method. J Photonic Mater Technol. 2018;4(1):33-38. doi: 10.11648/j.jmpt.20180401.16

Copy | Download

@article{10.11648/j.jmpt.20180401.16,
  author = {Li Chen and Baosheng Liu and Chundan Wang and Lihua Ma and Hongcai Zhang and Xu Guo},
  title = {Mechanism of Interaction Between Cefepime and Papain by Spectroscopic Method},
  journal = {Journal of Photonic Materials and Technology},
  volume = {4},
  number = {1},
  pages = {33-38},
  doi = {10.11648/j.jmpt.20180401.16},
  url = {https://doi.org/10.11648/j.jmpt.20180401.16},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jmpt.20180401.16},
  abstract = {The reaction mechanism of cefepime (CEF) with papain (PAPA) was investigated by both fluorescence quenching and synchronous fluorescence spectroscopy in different temperature (293, 308 and 318 K). The results demonstrated that the interaction between CEF and PAPA was taking place via static quenching. Thermodynamic parameters revealed that electrostatic interaction played major roles in the interaction. The order of magnitudes of binding constant is 104, and the number of binding site in the system was closed to 1. The binding rates of CEF to PAPA (λex = 280 nm) were 81.46%~95.95% (293K), 80.50%~95.49% (308K) and 79.77%~95.12% (318K). There was a linear relationship between ΔF and the concentrations of CEF in the range of 6.0×10-6~7.0×10-5 mol/L. And the correlation coefficient of the linear curve was 0.9973.},
 year = {2018}
}

Copy | Download

TY  - JOUR
T1  - Mechanism of Interaction Between Cefepime and Papain by Spectroscopic Method
AU  - Li Chen
AU  - Baosheng Liu
AU  - Chundan Wang
AU  - Lihua Ma
AU  - Hongcai Zhang
AU  - Xu Guo
Y1  - 2018/03/19
PY  - 2018
N1  - https://doi.org/10.11648/j.jmpt.20180401.16
DO  - 10.11648/j.jmpt.20180401.16
T2  - Journal of Photonic Materials and Technology
JF  - Journal of Photonic Materials and Technology
JO  - Journal of Photonic Materials and Technology
SP  - 33
EP  - 38
PB  - Science Publishing Group
SN  - 2469-8431
UR  - https://doi.org/10.11648/j.jmpt.20180401.16
AB  - The reaction mechanism of cefepime (CEF) with papain (PAPA) was investigated by both fluorescence quenching and synchronous fluorescence spectroscopy in different temperature (293, 308 and 318 K). The results demonstrated that the interaction between CEF and PAPA was taking place via static quenching. Thermodynamic parameters revealed that electrostatic interaction played major roles in the interaction. The order of magnitudes of binding constant is 104, and the number of binding site in the system was closed to 1. The binding rates of CEF to PAPA (λex = 280 nm) were 81.46%~95.95% (293K), 80.50%~95.49% (308K) and 79.77%~95.12% (318K). There was a linear relationship between ΔF and the concentrations of CEF in the range of 6.0×10-6~7.0×10-5 mol/L. And the correlation coefficient of the linear curve was 0.9973.
VL  - 4
IS  - 1
ER  -

Copy | Download