Comparison of P53 Marker Expression Between Dentigerous Cyst and Calcifying Odontogenic Cyst

Samira Mostafazadeh; Babak Ebrahimi; Fariba Abdal

doi:doi:10.11648/j.sf.20250604.12

Research Article |

| Peer-Reviewed

Comparison of P53 Marker Expression Between Dentigerous Cyst and Calcifying Odontogenic Cyst

Samira Mostafazadeh

, Babak Ebrahimi, Fariba Abdal^*

Published in Science Frontiers (Volume 6, Issue 4)

Received: 30 October 2025 Accepted: 10 November 2025 Published: 9 December 2025

Views: Downloads:

Download PDF

Share This Article

Twitter
Linked In
Facebook

Abstract

Background: Calcifying odontogenic cyst is a developmental cyst that often appears in jaw cysts, and dentigerous cyst is the most common developmental cyst of the jaw, which has the ability to turn into a neoplasm. p53 is increased in some odontogenic cysts and tumors. The aim of this study is to compare the expression of P53 marker in dentigerous cyst and calcifying odontogenic cyst by immunohistochemical method. Methods: In this descriptive-analytic cross-sectional study, the studied population is the sample of odontogenic dentigerous cysts and calcifying odontogenic cysts in the pathology center of urmia. First, all the files in the archive of the department from 2000 to 2021 were examined and the cases of cysts were examined separately and in terms of the expression of P53 marker in dentigerous cysts and calcifying odontogenic cysts by immunohistochemical method. Results: The results showed that the overall mean expression of p-53 marker in dentigerous cyst group was 33.45 with a standard deviation of 11.86%, among which the lowest and highest expression of the said marker was 12% and 54%, respectively. The overall average expression of p-53 marker in the calcifying odontogenic cyst group is 59.55 with a standard deviation of 13.73%, among which the lowest and highest expression of the said marker was 30% and 87%, respectively. There is a statistically significant difference between dentigerous cyst patients and odontogenic calcifying cyst patients in terms of p-53 marker expression, and the said marker is more expressed in patients in the odontogenic calcifying cyst group compared to dentigerous cyst patients (p< 0.001) Conclusions: It seems that this protein (p53) can probably be a suitable marker to confirm the ability to predict invasion in premalignant oral lesions such as cysts in odontogenic calcification patients.

Published in	Science Frontiers (Volume 6, Issue 4)
DOI	10.11648/j.sf.20250604.12
Page(s)	133-139
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2025. Published by Science Publishing Group

Keywords

P53 Marker, Denttiigerous Cyst, Calcifying Odontogenic Cyst, Immunohistochemical

References

[1]	Kamboj M, Juneja M. Ameloblastomatous Gorlin' s cyst. Journal of oral science. 2007; 49(4): 319-23. https://doi.org/10.2334/josnusd.49.319
[2]	Rajkumar K, Kamal K, Sathish M, Leena S. Calcifying odontogenic cyst. Journal of Oral and Maxillofacial Pathology. 2004; 8(2): 99.
[3]	Toida M. So‐called calcifying odontogenic cyst: review and discussion on the terminology and classification. Journal of oral pathology & medicine. 1998; 27(2): 49-52. https://doi.org/10.1111/j.1600-0714.1998.tb02092.x
[4]	Saghafi S, Zare-Mahmoodabadi R, Salehinejad J, Kadeh H, Afzal-Aghaee M. Immunohistochemical analysis of p53 and PCNA expression in calcifying odontogenic cyst. Journal of oral science. 2010; 52(4): 609-13. https://doi.org/10.2334/josnusd.52.609
[5]	Seyedmajidi M, Nafarzadeh S, Siadati S, Shafaee S, Bijani A, Keshmiri N. p53 and PCNA expression in keratocystic odontogenic tumors compared with selected odontogenic cysts. International journal of molecular and cellular medicine. 2013; 2(4): 185. URL: http://ijmcmed.org/article-1-105-en.html
[6]	AHN SG, KIM SA, KIM SG, LEE SH, Kim J, YOON JH. β‐catenin gene alterations in a variety of so‐called calcifying odontogenic cysts. Apmis. 2008; 116(3): 206-11. https://doi.org/10.1111/j.1600-0463.2008.00893.x
[7]	Prætorius F, Hjørting-Hansen E, Gorlin RJ, Vickers RA. Calcifying odontogenic cyst: range, variations and neoplastic potential. Acta Odontologica Scandinavica. 1981; 39(4): 227-40. https://doi.org/10.3109/00016358109162284
[8]	Fatemeh M, Sepideh A, Sara BS, Nazanin M. P53 protein expression in dental follicle, dentigerous cyst, odontogenic keratocyst, and inflammatory subtypes of cysts: An immunohistochemical study. Oman medical journal. 2017; 32(3): 227. https://doi.org/10.5001/omj.2017.42
[9]	Bernick S. Dentigerous cysts of the jaw. Oral Surgery, Oral Medicine, Oral Pathology. 1949; 2(7): 914-21. https://doi.org/10.1016/0030-4220(49)90234-0
[10]	Knights E, Brokaw W, Kessler H. The incidence of dentigerous cysts associated with a random sampling of unerupted third molars. Gen Dent. 1991; 39(2): 96-8.
[11]	Daley TD, Wysocki GP. The small dentigerous cyst: a diagnostic dilemma. Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontology. 1995; 79(1): 77-81. https://doi.org/10.1016/s1079-2104(05)80078-2
[12]	Shafer WG. A text book of oral pathology. Cherubism. 1983: 699-702.
[13]	Veera SD, Padanad G. Dentigerous cyst with recurrent maxillary sinusitis: a case report with literature review. Int J Appl Dent Sci. 2015; 1(4): 16-9.
[14]	Wang C-J, Huang P-H, Wang Y-L, Shyng Y-C, Kao W-B. Dentigerous cyst over maxillary sinus: A case report and literature review. 2009; 20(2): 116-24.
[15]	Khambete N, Kumar R, Risbud M, Kale L, Sodhi S. Dentigerous cyst associated with an impacted mesiodens: report of 2 cases. Imaging Science in Dentistry. 2012; 42(4): 255-60. https://doi.org/10.5624/isd.2012.42.4.255
[16]	Gaballah ETM, Tawfik MA. Immunohistochemical analysis of P53 protein in odontogenic cysts. The Saudi Dental Journal. 2010; 22(4): 167-70. https://doi.org/10.1016/j.sdentj.2010.07.002
[17]	Olson M, Dundr M. The moving parts of the nucleolus. Histochemistry and cell biology. 2005; 123(3): 203-16. https://doi.org/10.1007/s00418-005-0754-9
[18]	Ayla Ö, Can T, Mehmet Y, Belir A, Gülçin E. Expression of the tumor suppressor gene p53 in odontogenic cysts. Balkan Journal of Stomatology. 2002; 6(1): 47-50.
[19]	Oliveira MGd, Lauxen IdS, Chaves ACM, Rados PV, Sant' Ana Filho M. Immunohistochemical analysis of the patterns of p53 and PCNA expression in odontogenic cystic lesions. Medicina oral, patología oral y cirugía bucal Valencia Vol 13, no 5 (May 2008), p e275-e280. 2008.
[20]	Suzuki T, Kumamoto H, Kunimori K, Ooya K. Immunohistochemical analysis of apoptosis‐related factors in lining epithelium of radicular cysts. Journal of oral pathology & medicine. 2005; 34(1): 46-52. https://doi.org/10.1111/j.1600-0714.2004.00248.x
[21]	Kichi E, Enokiya Y, Muramatsu T, Hashimoto S, Inoue T, Abiko Y, et al. Cell proliferation, apoptosis and apoptosis‐related factors in odontogenic keratocysts and in dentigerous cysts. Journal of oral pathology & medicine. 2005; 34(5): 280-6. https://doi.org/10.1111/j.1600-0714.2005.00314.x
[22]	Piattelli A, Fioroni M, Santinelli A, Rubini C. P53 protein expression in odontogenic cysts. Journal of endodontics. 2001; 27(7): 459-61. https://doi.org/10.1097/00004770-200107000-00006
[23]	Shojaee S, Jamshidi S, Mohtasham N, Roshanaee G, Shahabinejad M. Evaluation of MDM2 and P53 Expression in Dentigerous Cyst and Odontogenic Keratocyst by Immunuhistochemistry. Journal of Mashhad Dental School. 2015; 39(2): 163-72. https://doi.org/10.22038/JMDS.2015.4471
[24]	Neville BW, Damm DD, Allen C, Chi AC. Oral and maxillofacial pathology: Elsevier Health Sciences; 2015.
[25]	KHalili M. Immunohistochemical investigation of p53 and ki67 proteins in samples of inflammatory and developmental odontogenic cysts. Iranian Journal of Dentistry. 2020; 22(3): 182-9.
[26]	Gurgel CAS, Ramos EAG, Azevedo RA, Sarmento VA, da Silva Carvalho AM, dos Santos JN. Expression of Ki-67, p53 and p63 proteins in keratocyst odontogenic tumours: an immunohistochemical study. Journal of molecular Histology. 2008; 39(3): 311-6. https://doi.org/10.1007/s10735-008-9167-0
[27]	Abou-Bakr AA, Abdelaziz AA, Malash IA, Mansour O, Abdelsalam IM, Abo-Elazm OM, et al. The Prognostic Significance of c-Met and p53 Immunohistochemical Expression in Gastric and Colorectal Carcinomas. Open Access Macedonian Journal of Medical Sciences. 2021; 9(A): 134-42. https://doi.org/10.3889/oamjms.2021.5910
[28]	Zydroń R, Marszałek A, Bodnar M, Kosikowski P, Greczka G, Wierzbicka M. The analysis of expression of p16 protein in group of 53 patients treated for sinonasal inverted papilloma. Brazilian Journal of Otorhinolaryngology. 2018; 84: 338-43. https://doi.org/10.1016/j.bjorl.2017.03.011
[29]	Kadashetti V, Patil N, Datkhile K, Kanetakar S, Shivakumar K. Analysis of expression of p53, p63 and proliferating cell nuclear antigen proteins in odontogenic keratocyst: An immunohistochemical study. Journal of Oral and Maxillofacial Pathology: JOMFP. 2020; 24(2): 273. https://doi.org/10.4103/jomfp.JOMFP_203_19
[30]	Gadbail A, Chaudhary M, Patil S, Gawande M. Actual Proliferating Index and p53 protein expression as prognostic marker in odontogenic cysts. Oral Diseases. 2009; 15(7): 490-8. https://doi.org/10.1111/j.1601-0825.2009.01590
[31]	Barreras CMU, Camou JGR, Burgueño ERR. P63/p40 expression in benign odontogenic cysts and tumors. Revista Odontológica Mexicana. 2022; 25(3): 215-23. https://doi.org/10.22201/fo.1870199xp.2021.25.3.83092

Cite This Article

Plain Text BibTeX RIS

APA Style

Mostafazadeh, S., Ebrahimi, B., Abdal, F. (2025). Comparison of P53 Marker Expression Between Dentigerous Cyst and Calcifying Odontogenic Cyst. Science Frontiers, 6(4), 133-139. https://doi.org/10.11648/j.sf.20250604.12

Copy | Download

ACS Style

Mostafazadeh, S.; Ebrahimi, B.; Abdal, F. Comparison of P53 Marker Expression Between Dentigerous Cyst and Calcifying Odontogenic Cyst. Sci. Front. 2025, 6(4), 133-139. doi: 10.11648/j.sf.20250604.12

Copy | Download

AMA Style

Mostafazadeh S, Ebrahimi B, Abdal F. Comparison of P53 Marker Expression Between Dentigerous Cyst and Calcifying Odontogenic Cyst. Sci Front. 2025;6(4):133-139. doi: 10.11648/j.sf.20250604.12

Copy | Download

@article{10.11648/j.sf.20250604.12,
  author = {Samira Mostafazadeh and Babak Ebrahimi and Fariba Abdal},
  title = {Comparison of P53 Marker Expression Between Dentigerous Cyst and Calcifying Odontogenic Cyst},
  journal = {Science Frontiers},
  volume = {6},
  number = {4},
  pages = {133-139},
  doi = {10.11648/j.sf.20250604.12},
  url = {https://doi.org/10.11648/j.sf.20250604.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.sf.20250604.12},
  abstract = {Background: Calcifying odontogenic cyst is a developmental cyst that often appears in jaw cysts, and dentigerous cyst is the most common developmental cyst of the jaw, which has the ability to turn into a neoplasm. p53 is increased in some odontogenic cysts and tumors. The aim of this study is to compare the expression of P53 marker in dentigerous cyst and calcifying odontogenic cyst by immunohistochemical method. Methods: In this descriptive-analytic cross-sectional study, the studied population is the sample of odontogenic dentigerous cysts and calcifying odontogenic cysts in the pathology center of urmia. First, all the files in the archive of the department from 2000 to 2021 were examined and the cases of cysts were examined separately and in terms of the expression of P53 marker in dentigerous cysts and calcifying odontogenic cysts by immunohistochemical method. Results: The results showed that the overall mean expression of p-53 marker in dentigerous cyst group was 33.45 with a standard deviation of 11.86%, among which the lowest and highest expression of the said marker was 12% and 54%, respectively. The overall average expression of p-53 marker in the calcifying odontogenic cyst group is 59.55 with a standard deviation of 13.73%, among which the lowest and highest expression of the said marker was 30% and 87%, respectively. There is a statistically significant difference between dentigerous cyst patients and odontogenic calcifying cyst patients in terms of p-53 marker expression, and the said marker is more expressed in patients in the odontogenic calcifying cyst group compared to dentigerous cyst patients (p< 0.001) Conclusions: It seems that this protein (p53) can probably be a suitable marker to confirm the ability to predict invasion in premalignant oral lesions such as cysts in odontogenic calcification patients.},
 year = {2025}
}

Copy | Download

TY  - JOUR
T1  - Comparison of P53 Marker Expression Between Dentigerous Cyst and Calcifying Odontogenic Cyst
AU  - Samira Mostafazadeh
AU  - Babak Ebrahimi
AU  - Fariba Abdal
Y1  - 2025/12/09
PY  - 2025
N1  - https://doi.org/10.11648/j.sf.20250604.12
DO  - 10.11648/j.sf.20250604.12
T2  - Science Frontiers
JF  - Science Frontiers
JO  - Science Frontiers
SP  - 133
EP  - 139
PB  - Science Publishing Group
SN  - 2994-7030
UR  - https://doi.org/10.11648/j.sf.20250604.12
AB  - Background: Calcifying odontogenic cyst is a developmental cyst that often appears in jaw cysts, and dentigerous cyst is the most common developmental cyst of the jaw, which has the ability to turn into a neoplasm. p53 is increased in some odontogenic cysts and tumors. The aim of this study is to compare the expression of P53 marker in dentigerous cyst and calcifying odontogenic cyst by immunohistochemical method. Methods: In this descriptive-analytic cross-sectional study, the studied population is the sample of odontogenic dentigerous cysts and calcifying odontogenic cysts in the pathology center of urmia. First, all the files in the archive of the department from 2000 to 2021 were examined and the cases of cysts were examined separately and in terms of the expression of P53 marker in dentigerous cysts and calcifying odontogenic cysts by immunohistochemical method. Results: The results showed that the overall mean expression of p-53 marker in dentigerous cyst group was 33.45 with a standard deviation of 11.86%, among which the lowest and highest expression of the said marker was 12% and 54%, respectively. The overall average expression of p-53 marker in the calcifying odontogenic cyst group is 59.55 with a standard deviation of 13.73%, among which the lowest and highest expression of the said marker was 30% and 87%, respectively. There is a statistically significant difference between dentigerous cyst patients and odontogenic calcifying cyst patients in terms of p-53 marker expression, and the said marker is more expressed in patients in the odontogenic calcifying cyst group compared to dentigerous cyst patients (p< 0.001) Conclusions: It seems that this protein (p53) can probably be a suitable marker to confirm the ability to predict invasion in premalignant oral lesions such as cysts in odontogenic calcification patients.
VL  - 6
IS  - 4
ER  -

Copy | Download

Author Information

Samira Mostafazadeh

Department of Oral and Maxillofacial Pathology, Urmia University of Medical Sciences, Urmia, Iran

Contact Email

http://orcid.org/0000-0002-8620-6127
Babak Ebrahimi

Faculty of Dentistry, Urmia University of Medical Sciences, Urmia, Iran

Contact Email
Fariba Abdal

Department of Oral and Maxillofacial Pathology, Ilam University of Medical Sciences, Ilam, Iran

Contact Email

http://orcid.org/0000-0002-9391-6354

Download PDF

Submit an Article

Sections

Plain Text BibTeX RIS

APA Style

Mostafazadeh, S., Ebrahimi, B., Abdal, F. (2025). Comparison of P53 Marker Expression Between Dentigerous Cyst and Calcifying Odontogenic Cyst. Science Frontiers, 6(4), 133-139. https://doi.org/10.11648/j.sf.20250604.12

Copy | Download

ACS Style

Mostafazadeh, S.; Ebrahimi, B.; Abdal, F. Comparison of P53 Marker Expression Between Dentigerous Cyst and Calcifying Odontogenic Cyst. Sci. Front. 2025, 6(4), 133-139. doi: 10.11648/j.sf.20250604.12

Copy | Download

AMA Style

Mostafazadeh S, Ebrahimi B, Abdal F. Comparison of P53 Marker Expression Between Dentigerous Cyst and Calcifying Odontogenic Cyst. Sci Front. 2025;6(4):133-139. doi: 10.11648/j.sf.20250604.12

Copy | Download

@article{10.11648/j.sf.20250604.12,
  author = {Samira Mostafazadeh and Babak Ebrahimi and Fariba Abdal},
  title = {Comparison of P53 Marker Expression Between Dentigerous Cyst and Calcifying Odontogenic Cyst},
  journal = {Science Frontiers},
  volume = {6},
  number = {4},
  pages = {133-139},
  doi = {10.11648/j.sf.20250604.12},
  url = {https://doi.org/10.11648/j.sf.20250604.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.sf.20250604.12},
  abstract = {Background: Calcifying odontogenic cyst is a developmental cyst that often appears in jaw cysts, and dentigerous cyst is the most common developmental cyst of the jaw, which has the ability to turn into a neoplasm. p53 is increased in some odontogenic cysts and tumors. The aim of this study is to compare the expression of P53 marker in dentigerous cyst and calcifying odontogenic cyst by immunohistochemical method. Methods: In this descriptive-analytic cross-sectional study, the studied population is the sample of odontogenic dentigerous cysts and calcifying odontogenic cysts in the pathology center of urmia. First, all the files in the archive of the department from 2000 to 2021 were examined and the cases of cysts were examined separately and in terms of the expression of P53 marker in dentigerous cysts and calcifying odontogenic cysts by immunohistochemical method. Results: The results showed that the overall mean expression of p-53 marker in dentigerous cyst group was 33.45 with a standard deviation of 11.86%, among which the lowest and highest expression of the said marker was 12% and 54%, respectively. The overall average expression of p-53 marker in the calcifying odontogenic cyst group is 59.55 with a standard deviation of 13.73%, among which the lowest and highest expression of the said marker was 30% and 87%, respectively. There is a statistically significant difference between dentigerous cyst patients and odontogenic calcifying cyst patients in terms of p-53 marker expression, and the said marker is more expressed in patients in the odontogenic calcifying cyst group compared to dentigerous cyst patients (p< 0.001) Conclusions: It seems that this protein (p53) can probably be a suitable marker to confirm the ability to predict invasion in premalignant oral lesions such as cysts in odontogenic calcification patients.},
 year = {2025}
}

Copy | Download

TY  - JOUR
T1  - Comparison of P53 Marker Expression Between Dentigerous Cyst and Calcifying Odontogenic Cyst
AU  - Samira Mostafazadeh
AU  - Babak Ebrahimi
AU  - Fariba Abdal
Y1  - 2025/12/09
PY  - 2025
N1  - https://doi.org/10.11648/j.sf.20250604.12
DO  - 10.11648/j.sf.20250604.12
T2  - Science Frontiers
JF  - Science Frontiers
JO  - Science Frontiers
SP  - 133
EP  - 139
PB  - Science Publishing Group
SN  - 2994-7030
UR  - https://doi.org/10.11648/j.sf.20250604.12
AB  - Background: Calcifying odontogenic cyst is a developmental cyst that often appears in jaw cysts, and dentigerous cyst is the most common developmental cyst of the jaw, which has the ability to turn into a neoplasm. p53 is increased in some odontogenic cysts and tumors. The aim of this study is to compare the expression of P53 marker in dentigerous cyst and calcifying odontogenic cyst by immunohistochemical method. Methods: In this descriptive-analytic cross-sectional study, the studied population is the sample of odontogenic dentigerous cysts and calcifying odontogenic cysts in the pathology center of urmia. First, all the files in the archive of the department from 2000 to 2021 were examined and the cases of cysts were examined separately and in terms of the expression of P53 marker in dentigerous cysts and calcifying odontogenic cysts by immunohistochemical method. Results: The results showed that the overall mean expression of p-53 marker in dentigerous cyst group was 33.45 with a standard deviation of 11.86%, among which the lowest and highest expression of the said marker was 12% and 54%, respectively. The overall average expression of p-53 marker in the calcifying odontogenic cyst group is 59.55 with a standard deviation of 13.73%, among which the lowest and highest expression of the said marker was 30% and 87%, respectively. There is a statistically significant difference between dentigerous cyst patients and odontogenic calcifying cyst patients in terms of p-53 marker expression, and the said marker is more expressed in patients in the odontogenic calcifying cyst group compared to dentigerous cyst patients (p< 0.001) Conclusions: It seems that this protein (p53) can probably be a suitable marker to confirm the ability to predict invasion in premalignant oral lesions such as cysts in odontogenic calcification patients.
VL  - 6
IS  - 4
ER  -

Copy | Download