The phytochemical investigation of a previously unstudied species of the genus Apocynaceae, Baissea mortehanii de Wild was undertaken and eight known secondary metabolites were isolated from leaves of this plant including one alkaloid, N-Feruloyltryptamine (1); one aromatic ester, Dibutyl phthalate (2); two flavonoids, Genistein (3) and Gerontoisoflavone A (4), four sterols, β-Sitosterol (5), Sitosterol-3-O-β-D-glucopyranoside (6), Stigmasterol (7) and Stigmasterol-3-O-β-D glucopyranoside (8). The structures of compounds were determined by means of spectroscopic methods :NMR analysis (1H and13C NMR, 1H-1H-COSY, HSQC, HMBC), spectrometric methods such as UV, IR, ESI-MS, EI, and by comparing their data with those reported in the literature. All the isolated compounds were tested for their potential to inhibit the enzyme urease. Urease activity was determined by measuring ammonia production using the indophenol method and thiourea was used as standard inhibitor of urease. Compounds 5 and 7 showed the best urease inhibition with an IC50 value 17. 2 and 18.5 µM respectively, which is higher than that of the potent inhibitor, thiourea (IC50 = 21.5 µM); Compounds 3, 4, 6 and 8 showed a good urease inhibition with an IC50 value 26.9, 29.7, 32.8 and 34.3 µM respectively; Compounds 1 and 2 showed a moderate urease inhibition with an IC50 value 49.1 and 46.8 µM respectively.
| Published in | Science Journal of Chemistry (Volume 12, Issue 1) |
| DOI | 10.11648/j.sjc.20241201.13 |
| Page(s) | 23-26 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
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Copyright © The Author(s), 2024. Published by Science Publishing Group |
Apocynaceae, Baissea Mortehanii, Enzyme Urease
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APA Style
Lidwine, N., Fanny-Aimee, E. M., Caroline, N. N., Julien, N. H., Tsopgni, W. D. T., et al. (2024). Enzyme Urease Activity and Phytochemical Investigation of the Leaves of Baissea Mortehanii (Apocynaceae). Science Journal of Chemistry, 12(1), 23-26. https://doi.org/10.11648/j.sjc.20241201.13
ACS Style
Lidwine, N.; Fanny-Aimee, E. M.; Caroline, N. N.; Julien, N. H.; Tsopgni, W. D. T., et al. Enzyme Urease Activity and Phytochemical Investigation of the Leaves of Baissea Mortehanii (Apocynaceae). Sci. J. Chem. 2024, 12(1), 23-26. doi: 10.11648/j.sjc.20241201.13
AMA Style
Lidwine N, Fanny-Aimee EM, Caroline NN, Julien NH, Tsopgni WDT, et al. Enzyme Urease Activity and Phytochemical Investigation of the Leaves of Baissea Mortehanii (Apocynaceae). Sci J Chem. 2024;12(1):23-26. doi: 10.11648/j.sjc.20241201.13
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Download@article{10.11648/j.sjc.20241201.13,
author = {Ngah Lidwine and Essombe Malolo Fanny-Aimee and Ngo Nyobe Caroline and Nko’o Henri Julien and Willifred Dongmo Tekapi Tsopgni and Etame Loe Gisele and Jean Duplex Wansi and Kamdem Waffo and Ndom Jean Claude and Mpondo Mpondo Emmanuel},
title = {Enzyme Urease Activity and Phytochemical Investigation of the Leaves of Baissea Mortehanii (Apocynaceae)},
journal = {Science Journal of Chemistry},
volume = {12},
number = {1},
pages = {23-26},
doi = {10.11648/j.sjc.20241201.13},
url = {https://doi.org/10.11648/j.sjc.20241201.13},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.sjc.20241201.13},
abstract = {The phytochemical investigation of a previously unstudied species of the genus Apocynaceae, Baissea mortehanii de Wild was undertaken and eight known secondary metabolites were isolated from leaves of this plant including one alkaloid, N-Feruloyltryptamine (1); one aromatic ester, Dibutyl phthalate (2); two flavonoids, Genistein (3) and Gerontoisoflavone A (4), four sterols, β-Sitosterol (5), Sitosterol-3-O-β-D-glucopyranoside (6), Stigmasterol (7) and Stigmasterol-3-O-β-D glucopyranoside (8). The structures of compounds were determined by means of spectroscopic methods :NMR analysis (1H and13C NMR, 1H-1H-COSY, HSQC, HMBC), spectrometric methods such as UV, IR, ESI-MS, EI, and by comparing their data with those reported in the literature. All the isolated compounds were tested for their potential to inhibit the enzyme urease. Urease activity was determined by measuring ammonia production using the indophenol method and thiourea was used as standard inhibitor of urease. Compounds 5 and 7 showed the best urease inhibition with an IC50 value 17. 2 and 18.5 µM respectively, which is higher than that of the potent inhibitor, thiourea (IC50 = 21.5 µM); Compounds 3, 4, 6 and 8 showed a good urease inhibition with an IC50 value 26.9, 29.7, 32.8 and 34.3 µM respectively; Compounds 1 and 2 showed a moderate urease inhibition with an IC50 value 49.1 and 46.8 µM respectively.
},
year = {2024}
}
TY - JOUR T1 - Enzyme Urease Activity and Phytochemical Investigation of the Leaves of Baissea Mortehanii (Apocynaceae) AU - Ngah Lidwine AU - Essombe Malolo Fanny-Aimee AU - Ngo Nyobe Caroline AU - Nko’o Henri Julien AU - Willifred Dongmo Tekapi Tsopgni AU - Etame Loe Gisele AU - Jean Duplex Wansi AU - Kamdem Waffo AU - Ndom Jean Claude AU - Mpondo Mpondo Emmanuel Y1 - 2024/02/21 PY - 2024 N1 - https://doi.org/10.11648/j.sjc.20241201.13 DO - 10.11648/j.sjc.20241201.13 T2 - Science Journal of Chemistry JF - Science Journal of Chemistry JO - Science Journal of Chemistry SP - 23 EP - 26 PB - Science Publishing Group SN - 2330-099X UR - https://doi.org/10.11648/j.sjc.20241201.13 AB - The phytochemical investigation of a previously unstudied species of the genus Apocynaceae, Baissea mortehanii de Wild was undertaken and eight known secondary metabolites were isolated from leaves of this plant including one alkaloid, N-Feruloyltryptamine (1); one aromatic ester, Dibutyl phthalate (2); two flavonoids, Genistein (3) and Gerontoisoflavone A (4), four sterols, β-Sitosterol (5), Sitosterol-3-O-β-D-glucopyranoside (6), Stigmasterol (7) and Stigmasterol-3-O-β-D glucopyranoside (8). The structures of compounds were determined by means of spectroscopic methods :NMR analysis (1H and13C NMR, 1H-1H-COSY, HSQC, HMBC), spectrometric methods such as UV, IR, ESI-MS, EI, and by comparing their data with those reported in the literature. All the isolated compounds were tested for their potential to inhibit the enzyme urease. Urease activity was determined by measuring ammonia production using the indophenol method and thiourea was used as standard inhibitor of urease. Compounds 5 and 7 showed the best urease inhibition with an IC50 value 17. 2 and 18.5 µM respectively, which is higher than that of the potent inhibitor, thiourea (IC50 = 21.5 µM); Compounds 3, 4, 6 and 8 showed a good urease inhibition with an IC50 value 26.9, 29.7, 32.8 and 34.3 µM respectively; Compounds 1 and 2 showed a moderate urease inhibition with an IC50 value 49.1 and 46.8 µM respectively. VL - 12 IS - 1 ER -
Department of Pharmaceutical Sciences, Faculty of Medicine and Pharmaceutical Sciences, University of Douala, Douala, Cameroon
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