Effect of Osteoblasts Cell Adhesion Behavior on Biomaterial Surfaces by Atomic Force Microscope

Afrina Khan Piya; Munshi Muhammad Raihan; Md Alamgir Hossain

doi:doi:10.11648/j.aas.20200501.11

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Effect of Osteoblasts Cell Adhesion Behavior on Biomaterial Surfaces by Atomic Force Microscope

Afrina Khan Piya, Munshi Muhammad Raihan, Md Alamgir Hossain

Published in Advances in Applied Sciences (Volume 5, Issue 1)

Received: 26 October 2019 Accepted: 29 November 2019 Published: 23 March 2020

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Abstract

Atomic force microscopy (AFM) is a great scientific invention that can visualize cell morphology in aqueous environment and provide information to investigate cell biomechanics at a high spatial resolution in a controlled environment with force sensitivity. Contemporary AFM techniques permit solving a number of problems of cell biomechanics due to synchronized evaluation of the local mechanical properties. For characterizing mechanical properties force spectroscopy is used that provides information on cellular structures including cytoskeleton structure and morphology. For the success of biomedical implant, the most crucial factor is biocompatibility and osteo-conductivity of the implant material that can be characterized by the change in mechanical properties of cellular filaments and nucleus determined by exploiting atomic force microscope techniques. Hydroxyapatite is a bioactive material in bio-ceramics, hence used for fillers, bone grafts and metallic implant coating. Recently developed HAp/amino acid fluorescent complexes could be a significant candidate to be used for dental implant, had shown antibacterial properties with visible light irradiation. This study aims at revealing murine osteoblasts cell (MC3T3) adhesion behavior on the HAp coating and HAp/amino acid complexes. The AFM revealed that no significant changes were observed in mechanical properties of the osteoblasts cells when adhered on electrochemically deposited HAp coating and HAp/amino acid ligands complex coating. SEM and EDX analysis revealed cell morphology were identical for HAp and HAp/amino acid ligands complex coating. Such characteristics are desirable for the success of implant biomaterial coating that can preserve both antibacterial property and cell adhesion behavior.

Published in	Advances in Applied Sciences (Volume 5, Issue 1)
DOI	10.11648/j.aas.20200501.11
Page(s)	1-10
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Dental Implant, Antibacterial Property, HAp/Amino Acid Complex, Atomic Force Microscopy (AFM), Cell Elasticity, CIP, Cytoskeleton Dynamics, Biomaterials

References

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APA Style

Afrina Khan Piya, Munshi Muhammad Raihan, Md Alamgir Hossain. (2020). Effect of Osteoblasts Cell Adhesion Behavior on Biomaterial Surfaces by Atomic Force Microscope. Advances in Applied Sciences, 5(1), 1-10. https://doi.org/10.11648/j.aas.20200501.11

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ACS Style

Afrina Khan Piya; Munshi Muhammad Raihan; Md Alamgir Hossain. Effect of Osteoblasts Cell Adhesion Behavior on Biomaterial Surfaces by Atomic Force Microscope. Adv. Appl. Sci. 2020, 5(1), 1-10. doi: 10.11648/j.aas.20200501.11

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AMA Style

Afrina Khan Piya, Munshi Muhammad Raihan, Md Alamgir Hossain. Effect of Osteoblasts Cell Adhesion Behavior on Biomaterial Surfaces by Atomic Force Microscope. Adv Appl Sci. 2020;5(1):1-10. doi: 10.11648/j.aas.20200501.11

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@article{10.11648/j.aas.20200501.11,
  author = {Afrina Khan Piya and Munshi Muhammad Raihan and Md Alamgir Hossain},
  title = {Effect of Osteoblasts Cell Adhesion Behavior on Biomaterial Surfaces by Atomic Force Microscope},
  journal = {Advances in Applied Sciences},
  volume = {5},
  number = {1},
  pages = {1-10},
  doi = {10.11648/j.aas.20200501.11},
  url = {https://doi.org/10.11648/j.aas.20200501.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.aas.20200501.11},
  abstract = {Atomic force microscopy (AFM) is a great scientific invention that can visualize cell morphology in aqueous environment and provide information to investigate cell biomechanics at a high spatial resolution in a controlled environment with force sensitivity. Contemporary AFM techniques permit solving a number of problems of cell biomechanics due to synchronized evaluation of the local mechanical properties. For characterizing mechanical properties force spectroscopy is used that provides information on cellular structures including cytoskeleton structure and morphology. For the success of biomedical implant, the most crucial factor is biocompatibility and osteo-conductivity of the implant material that can be characterized by the change in mechanical properties of cellular filaments and nucleus determined by exploiting atomic force microscope techniques. Hydroxyapatite is a bioactive material in bio-ceramics, hence used for fillers, bone grafts and metallic implant coating. Recently developed HAp/amino acid fluorescent complexes could be a significant candidate to be used for dental implant, had shown antibacterial properties with visible light irradiation. This study aims at revealing murine osteoblasts cell (MC3T3) adhesion behavior on the HAp coating and HAp/amino acid complexes. The AFM revealed that no significant changes were observed in mechanical properties of the osteoblasts cells when adhered on electrochemically deposited HAp coating and HAp/amino acid ligands complex coating. SEM and EDX analysis revealed cell morphology were identical for HAp and HAp/amino acid ligands complex coating. Such characteristics are desirable for the success of implant biomaterial coating that can preserve both antibacterial property and cell adhesion behavior.},
 year = {2020}
}

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TY  - JOUR
T1  - Effect of Osteoblasts Cell Adhesion Behavior on Biomaterial Surfaces by Atomic Force Microscope
AU  - Afrina Khan Piya
AU  - Munshi Muhammad Raihan
AU  - Md Alamgir Hossain
Y1  - 2020/03/23
PY  - 2020
N1  - https://doi.org/10.11648/j.aas.20200501.11
DO  - 10.11648/j.aas.20200501.11
T2  - Advances in Applied Sciences
JF  - Advances in Applied Sciences
JO  - Advances in Applied Sciences
SP  - 1
EP  - 10
PB  - Science Publishing Group
SN  - 2575-1514
UR  - https://doi.org/10.11648/j.aas.20200501.11
AB  - Atomic force microscopy (AFM) is a great scientific invention that can visualize cell morphology in aqueous environment and provide information to investigate cell biomechanics at a high spatial resolution in a controlled environment with force sensitivity. Contemporary AFM techniques permit solving a number of problems of cell biomechanics due to synchronized evaluation of the local mechanical properties. For characterizing mechanical properties force spectroscopy is used that provides information on cellular structures including cytoskeleton structure and morphology. For the success of biomedical implant, the most crucial factor is biocompatibility and osteo-conductivity of the implant material that can be characterized by the change in mechanical properties of cellular filaments and nucleus determined by exploiting atomic force microscope techniques. Hydroxyapatite is a bioactive material in bio-ceramics, hence used for fillers, bone grafts and metallic implant coating. Recently developed HAp/amino acid fluorescent complexes could be a significant candidate to be used for dental implant, had shown antibacterial properties with visible light irradiation. This study aims at revealing murine osteoblasts cell (MC3T3) adhesion behavior on the HAp coating and HAp/amino acid complexes. The AFM revealed that no significant changes were observed in mechanical properties of the osteoblasts cells when adhered on electrochemically deposited HAp coating and HAp/amino acid ligands complex coating. SEM and EDX analysis revealed cell morphology were identical for HAp and HAp/amino acid ligands complex coating. Such characteristics are desirable for the success of implant biomaterial coating that can preserve both antibacterial property and cell adhesion behavior.
VL  - 5
IS  - 1
ER  -

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Author Information

Afrina Khan Piya

Department of Mechanical Engineering, Nagaoka University of Technology, Nagaoka City, Japan
Munshi Muhammad Raihan

Department of Mechanical Engineering, Nagaoka University of Technology, Nagaoka City, Japan
Md Alamgir Hossain

Department of Mechanical Engineering, Military Institute of Science and Technology, Dhaka, Bangladesh

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APA Style

Afrina Khan Piya, Munshi Muhammad Raihan, Md Alamgir Hossain. (2020). Effect of Osteoblasts Cell Adhesion Behavior on Biomaterial Surfaces by Atomic Force Microscope. Advances in Applied Sciences, 5(1), 1-10. https://doi.org/10.11648/j.aas.20200501.11

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ACS Style

Afrina Khan Piya; Munshi Muhammad Raihan; Md Alamgir Hossain. Effect of Osteoblasts Cell Adhesion Behavior on Biomaterial Surfaces by Atomic Force Microscope. Adv. Appl. Sci. 2020, 5(1), 1-10. doi: 10.11648/j.aas.20200501.11

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AMA Style

Afrina Khan Piya, Munshi Muhammad Raihan, Md Alamgir Hossain. Effect of Osteoblasts Cell Adhesion Behavior on Biomaterial Surfaces by Atomic Force Microscope. Adv Appl Sci. 2020;5(1):1-10. doi: 10.11648/j.aas.20200501.11

Copy | Download

@article{10.11648/j.aas.20200501.11,
  author = {Afrina Khan Piya and Munshi Muhammad Raihan and Md Alamgir Hossain},
  title = {Effect of Osteoblasts Cell Adhesion Behavior on Biomaterial Surfaces by Atomic Force Microscope},
  journal = {Advances in Applied Sciences},
  volume = {5},
  number = {1},
  pages = {1-10},
  doi = {10.11648/j.aas.20200501.11},
  url = {https://doi.org/10.11648/j.aas.20200501.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.aas.20200501.11},
  abstract = {Atomic force microscopy (AFM) is a great scientific invention that can visualize cell morphology in aqueous environment and provide information to investigate cell biomechanics at a high spatial resolution in a controlled environment with force sensitivity. Contemporary AFM techniques permit solving a number of problems of cell biomechanics due to synchronized evaluation of the local mechanical properties. For characterizing mechanical properties force spectroscopy is used that provides information on cellular structures including cytoskeleton structure and morphology. For the success of biomedical implant, the most crucial factor is biocompatibility and osteo-conductivity of the implant material that can be characterized by the change in mechanical properties of cellular filaments and nucleus determined by exploiting atomic force microscope techniques. Hydroxyapatite is a bioactive material in bio-ceramics, hence used for fillers, bone grafts and metallic implant coating. Recently developed HAp/amino acid fluorescent complexes could be a significant candidate to be used for dental implant, had shown antibacterial properties with visible light irradiation. This study aims at revealing murine osteoblasts cell (MC3T3) adhesion behavior on the HAp coating and HAp/amino acid complexes. The AFM revealed that no significant changes were observed in mechanical properties of the osteoblasts cells when adhered on electrochemically deposited HAp coating and HAp/amino acid ligands complex coating. SEM and EDX analysis revealed cell morphology were identical for HAp and HAp/amino acid ligands complex coating. Such characteristics are desirable for the success of implant biomaterial coating that can preserve both antibacterial property and cell adhesion behavior.},
 year = {2020}
}

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TY  - JOUR
T1  - Effect of Osteoblasts Cell Adhesion Behavior on Biomaterial Surfaces by Atomic Force Microscope
AU  - Afrina Khan Piya
AU  - Munshi Muhammad Raihan
AU  - Md Alamgir Hossain
Y1  - 2020/03/23
PY  - 2020
N1  - https://doi.org/10.11648/j.aas.20200501.11
DO  - 10.11648/j.aas.20200501.11
T2  - Advances in Applied Sciences
JF  - Advances in Applied Sciences
JO  - Advances in Applied Sciences
SP  - 1
EP  - 10
PB  - Science Publishing Group
SN  - 2575-1514
UR  - https://doi.org/10.11648/j.aas.20200501.11
AB  - Atomic force microscopy (AFM) is a great scientific invention that can visualize cell morphology in aqueous environment and provide information to investigate cell biomechanics at a high spatial resolution in a controlled environment with force sensitivity. Contemporary AFM techniques permit solving a number of problems of cell biomechanics due to synchronized evaluation of the local mechanical properties. For characterizing mechanical properties force spectroscopy is used that provides information on cellular structures including cytoskeleton structure and morphology. For the success of biomedical implant, the most crucial factor is biocompatibility and osteo-conductivity of the implant material that can be characterized by the change in mechanical properties of cellular filaments and nucleus determined by exploiting atomic force microscope techniques. Hydroxyapatite is a bioactive material in bio-ceramics, hence used for fillers, bone grafts and metallic implant coating. Recently developed HAp/amino acid fluorescent complexes could be a significant candidate to be used for dental implant, had shown antibacterial properties with visible light irradiation. This study aims at revealing murine osteoblasts cell (MC3T3) adhesion behavior on the HAp coating and HAp/amino acid complexes. The AFM revealed that no significant changes were observed in mechanical properties of the osteoblasts cells when adhered on electrochemically deposited HAp coating and HAp/amino acid ligands complex coating. SEM and EDX analysis revealed cell morphology were identical for HAp and HAp/amino acid ligands complex coating. Such characteristics are desirable for the success of implant biomaterial coating that can preserve both antibacterial property and cell adhesion behavior.
VL  - 5
IS  - 1
ER  -

Copy | Download