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Green Synthesis and Antibacterial Properties of Silver Nanoparticles Using Sudanese Azadirachta indica Leaves Extract

Received: Jan. 26, 2020    Accepted: Feb. 19, 2020    Published: Apr. 28, 2020
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Abstract

Nanoparticles have emerged as a key player in modern medicine and involved in drug delivery, it can be manufactured using various approaches including chemical, physical, and biological methods. Nanoparticles are generally characterized by their size, morphology and surface charge using advanced microscopic techniques such as SEM and TEM. This study was aimed for synthesis and characterization of silver nanoparticle and to detect their antibacterial activity using Azadirachta indica (Neem) leaves extract. The results showed a visible change of colour after the addition of Neem leaf extract to AgNO3 solution to dark brown which indicates the formation of silver nanoparticle. UV spectrophotometer showed absorbance peak in range of 390-700 nm, the result revealed the highest peak showed by concentration 1 ml at 428.80 nm. SEM and XRD analysis were used to characterize synthesize silver nanoparticles. The antibacterial activity against Gram positive Staphylococcus aureus and Gram negative Escherichia coli and Klebsiella spp Microorganisms was carried out using disc diffusion method, the results showed that the highest activity was 14 mm against S. aureus. A. indica silver nanoparticles can be used as a new nano drug in formulation and delivery. The synthesized silver nanoparticles are extremely important in various areas of life and are widely used for the benefit of human being.

DOI 10.11648/j.sr.20200801.13
Published in Science Research ( Volume 8, Issue 1, February 2020 )
Page(s) 14-19
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Green Synthesis, Silver Nanoparticles, Azadirachta indica, SEM, XRD, Antibacterial Properties, Sudan

References
[1] Sriram, M. I., Kalishwaralal, K., Barathmanikanth, S and Gurunathani, S. (2012). Size-based cytotoxicity of silver nanoparticles in bovine retinal endothelial cells. Nanosci Methods; 1 (1): 56–77.
[2] Riley, M. A., Robinson, S. M., Roy, C. M., Dennis, M., Liu, V., Dorit, R. L. (2012). Resistance is futile: the bacteriocin model for addressing the antibiotic resistance challenge. Biochem Soc Trans. 40 (6): 1438–1442.
[3] Ovais, M., Khalil, A. T., Raza, A., Khan, M. A., Ahmad, I., Islam, N. U., Saravanan, M., Ubaid, M. F., Ali, M and Shinwari, Z. K. (2016). Green synthesis of silver nanoparticles via plant extracts: beginning a new era in cancer theranostics. Nanomedicine; 12 (23): 3157-77.
[4] Khan, M., Adil, S. F., Musarrat, J., Al-Khedhairy, A., Al-Warthan, A., HSiddiqui, M. R., Rafiq, M and Alkhathlan, H. (2014). Antibacterial properties of silver nanoparticles synthesized using Pulicaria glutinosa plant extract as a green bioreductant, Mujeeb Khan Shams Tabrez Khan, International Journal of Nanomedicine; 2014: 9 3551–3565.
[5] Bulent, U., Turkdemir, M. H., Cicek, A and Mete, A. (2015). Role of irradiation in the green synthesis of silver nanoparticles mediated by fig (Ficus carica) leaf extract. Spectrochim Acta Part A Mol Biomol Spectrosc; 135: 153–161.
[6] Siddiqi, K. S., Husen, A., Rao, R. A. K. (2018). A review on biosynthesis of silver nanoparticles and their biocidal properties. J Nanobiotechnol; 16: 14.
[7] Karar, M. G and Kuhnert, N. (2017). Herbal drugs from Sudan: Traditional uses and phytoconstituents. Pharmacognosy reviews; 11 (22): 83.
[8] Abdeen, S., Isaac, R. R., Geo, S., Sornalekshmi, S., Rose, A and Praseetha, P. K. (2013). Evaluation of Antimicrobial Activity of Biosynthesized Iron and Silver Nanoparticles Using the Fungi Fusarium Oxysporum and Actinomycetes sp. on Human Pathogens. Nano Biomedicine & Engineering; 1; 5 (1).
[9] Mahfuzul Hoque, M. D., Bari, M. L., Inatsu, Y., Juneja, V. K. and Kawamoto, S. (2007). Antibacterial activity of guava (Psidium guajava L.) and neem (Azadirachta indica A. Juss.) extracts against foodborne pathogens and spoilage bacteria. Foodborne pathogens and disease. Dec 1; 4 (4): 481-8.
[10] Veerasamy, R., Xin, T. Z., Gunasagaran, S., Xiang, T. F., Yang, E. F., Jeyakumar, N. and Dhanaraj, S. A. (2011). Biosynthesis of silver nanoparticles using mangosteen leaf extract and evaluation of their antimicrobial activities. Journal of Saudi Chemical Society; 1; 15 (2): 113-20.
[11] Vijayakumar, M., Priya, K., Nancy, F. T., Noorlidah, A. and Ahmed, A. B. A. (2013). Biosynthesis, characterisation and anti-bacterial effect of plant-mediated silver nanoparticles using Artemisia nilagirica. Industrial Crops and Products, 41, 235-240.
[12] Bindhu, M. R., &Umadevi, M. (2013). Synthesis of monodispersed silver nanoparticles using Hibiscus cannabinus leaf extract and its antimicrobial activity. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 101, 184-190.
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  • APA Style

    Ayat Ahmed Alrasheid, Sahar Hussein Eltilib, Shimaa Abdel Rahman Ahmed, Alaa Abdulmoneim Mohamed, Hiba Abbas Widatalla, et al. (2020). Green Synthesis and Antibacterial Properties of Silver Nanoparticles Using Sudanese Azadirachta indica Leaves Extract. Science Research, 8(1), 14-19. https://doi.org/10.11648/j.sr.20200801.13

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    ACS Style

    Ayat Ahmed Alrasheid; Sahar Hussein Eltilib; Shimaa Abdel Rahman Ahmed; Alaa Abdulmoneim Mohamed; Hiba Abbas Widatalla, et al. Green Synthesis and Antibacterial Properties of Silver Nanoparticles Using Sudanese Azadirachta indica Leaves Extract. Sci. Res. 2020, 8(1), 14-19. doi: 10.11648/j.sr.20200801.13

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    AMA Style

    Ayat Ahmed Alrasheid, Sahar Hussein Eltilib, Shimaa Abdel Rahman Ahmed, Alaa Abdulmoneim Mohamed, Hiba Abbas Widatalla, et al. Green Synthesis and Antibacterial Properties of Silver Nanoparticles Using Sudanese Azadirachta indica Leaves Extract. Sci Res. 2020;8(1):14-19. doi: 10.11648/j.sr.20200801.13

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  • @article{10.11648/j.sr.20200801.13,
      author = {Ayat Ahmed Alrasheid and Sahar Hussein Eltilib and Shimaa Abdel Rahman Ahmed and Alaa Abdulmoneim Mohamed and Hiba Abbas Widatalla and Layla Fathi Yassin and Maida Musa Ali and Marvit Osman Widdatallh},
      title = {Green Synthesis and Antibacterial Properties of Silver Nanoparticles Using Sudanese Azadirachta indica Leaves Extract},
      journal = {Science Research},
      volume = {8},
      number = {1},
      pages = {14-19},
      doi = {10.11648/j.sr.20200801.13},
      url = {https://doi.org/10.11648/j.sr.20200801.13},
      eprint = {https://download.sciencepg.com/pdf/10.11648.j.sr.20200801.13},
      abstract = {Nanoparticles have emerged as a key player in modern medicine and involved in drug delivery, it can be manufactured using various approaches including chemical, physical, and biological methods. Nanoparticles are generally characterized by their size, morphology and surface charge using advanced microscopic techniques such as SEM and TEM. This study was aimed for synthesis and characterization of silver nanoparticle and to detect their antibacterial activity using Azadirachta indica (Neem) leaves extract. The results showed a visible change of colour after the addition of Neem leaf extract to AgNO3 solution to dark brown which indicates the formation of silver nanoparticle. UV spectrophotometer showed absorbance peak in range of 390-700 nm, the result revealed the highest peak showed by concentration 1 ml at 428.80 nm. SEM and XRD analysis were used to characterize synthesize silver nanoparticles. The antibacterial activity against Gram positive Staphylococcus aureus and Gram negative Escherichia coli and Klebsiella spp Microorganisms was carried out using disc diffusion method, the results showed that the highest activity was 14 mm against S. aureus. A. indica silver nanoparticles can be used as a new nano drug in formulation and delivery. The synthesized silver nanoparticles are extremely important in various areas of life and are widely used for the benefit of human being.},
     year = {2020}
    }
    

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  • TY  - JOUR
    T1  - Green Synthesis and Antibacterial Properties of Silver Nanoparticles Using Sudanese Azadirachta indica Leaves Extract
    AU  - Ayat Ahmed Alrasheid
    AU  - Sahar Hussein Eltilib
    AU  - Shimaa Abdel Rahman Ahmed
    AU  - Alaa Abdulmoneim Mohamed
    AU  - Hiba Abbas Widatalla
    AU  - Layla Fathi Yassin
    AU  - Maida Musa Ali
    AU  - Marvit Osman Widdatallh
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    N1  - https://doi.org/10.11648/j.sr.20200801.13
    DO  - 10.11648/j.sr.20200801.13
    T2  - Science Research
    JF  - Science Research
    JO  - Science Research
    SP  - 14
    EP  - 19
    PB  - Science Publishing Group
    SN  - 2329-0927
    UR  - https://doi.org/10.11648/j.sr.20200801.13
    AB  - Nanoparticles have emerged as a key player in modern medicine and involved in drug delivery, it can be manufactured using various approaches including chemical, physical, and biological methods. Nanoparticles are generally characterized by their size, morphology and surface charge using advanced microscopic techniques such as SEM and TEM. This study was aimed for synthesis and characterization of silver nanoparticle and to detect their antibacterial activity using Azadirachta indica (Neem) leaves extract. The results showed a visible change of colour after the addition of Neem leaf extract to AgNO3 solution to dark brown which indicates the formation of silver nanoparticle. UV spectrophotometer showed absorbance peak in range of 390-700 nm, the result revealed the highest peak showed by concentration 1 ml at 428.80 nm. SEM and XRD analysis were used to characterize synthesize silver nanoparticles. The antibacterial activity against Gram positive Staphylococcus aureus and Gram negative Escherichia coli and Klebsiella spp Microorganisms was carried out using disc diffusion method, the results showed that the highest activity was 14 mm against S. aureus. A. indica silver nanoparticles can be used as a new nano drug in formulation and delivery. The synthesized silver nanoparticles are extremely important in various areas of life and are widely used for the benefit of human being.
    VL  - 8
    IS  - 1
    ER  - 

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Author Information
  • Department of Pharmacognosy, Faculty of Pharmacy, University of Medical Sciences and Technology, Khartoum, Sudan

  • Department of Pharmacology, Faculty of Pharmacy, University of Medical Sciences and Technology, Khartoum, Sudan

  • Department of Pharmaceutical Analysis, Faculty of Pharmacy, University of Medical Sciences and Technology, Khartoum, Sudan

  • Department of Clinical Pharmacy, Faculty of Pharmacy, University of Medical Sciences and Technology, Khartoum, Sudan

  • Department of Pharmacology, Faculty of Pharmacy, University of Medical Sciences and Technology, Khartoum, Sudan

  • Department of Pharmaceutical Analysis, Faculty of Pharmacy, University of Medical Sciences and Technology, Khartoum, Sudan

  • Department of Pharmaceutical Analysis, Faculty of Pharmacy, University of Medical Sciences and Technology, Khartoum, Sudan

  • Department of Pharmacology, Faculty of Pharmacy, University of Medical Sciences and Technology, Khartoum, Sudan

  • Section