Microbiological Analysis of Top Soil and Rhizosphere Treated with Organic Manure

Aniefon Alphonsus Ibuot; Iniobong Ime James; Mayen Godwin Ben; Christiana Utibe Etuk; Agnes Monday Jones; Emmanuel Anthony Umoren; Elizabeth Lazarus Akpan; Esther Ndarake Akpan

doi:doi:10.11648/j.abb.20190702.13

Microbiological Analysis of Top Soil and Rhizosphere Treated with Organic Manure

Aniefon Alphonsus Ibuot, Iniobong Ime James, Mayen Godwin Ben, Christiana Utibe Etuk, Agnes Monday Jones, Emmanuel Anthony Umoren, Elizabeth Lazarus Akpan, Esther Ndarake Akpan

Published in Advances in Bioscience and Bioengineering (Volume 7, Issue 2)

Received: 6 June 2019 Accepted: 12 July 2019 Published: 26 July 2019

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Abstract

Microbiological analysis of topsoil and rhizosphere treated with organic manure (Poultry droppings) was carried out. Soil samples were analyzed at three days interval (Day 1, Day 4, Day 7 and Day 10). The total bacterial count recorded for rhizosphere soil treated with poultry droppings (RS:P) had the highest bacterial count ranging between 2.1×10⁶ and 5.7×10⁶ CFU/g. Top soil treated with poultry droppings had total bacterial count ranging between 1.9×10⁶ and 4.9×10⁶ CFU/g. The controls (untreated rhizosphere soil) had a total bacterial count ranging between 2.6×10⁶ and 4.0×10⁶ CFU/g and untreated topsoil had bacterial count ranging between 1.4×10⁶ and 2.4×10⁶ CFU/g. Total fungal count for top soil treated with poultry droppings ranged between 0.2×10⁶ and 0.9×10⁶ CFU/g. Total fungal count for rhizosphere soil treated with poultry droppings ranged between 0.2×10⁶ and 0.3×10⁶ CFU/g. Untreated top soil had total fungal count ranging between 0.1×10⁶ and 0.2×10⁶ CFU/g while untreated rhizosphere soil had a total fungal count ranging between 0.1×10⁶ and 0.2×10⁶ CFU/g. Bacterial isolates identified with their percentage frequency of occurrence were Bacillus sp (16.8), Enterococus sp (8.4), Clostridium sp (4.0), Staphylococcus sp (8.0) Pseudomonas sp (15.6), Listeria sp (12.0), Micrococcus sp (14.0), Serratia sp (4.8) and Streptococcus sp (7.2). Fungal isolates identified with their percentage frequency of occurrence were Rhizosphere sp (26.7%), Penicillium sp (22.5%), Aspergillus sp (21.1%), Mucor sp (19.7%) and lastly Cladosporium sp (9.8%). Metabolites secreted by the root system act as chemical signal attracting high population of microorganisms. The application of organic manure to the soil enhanced the microbial population of the soil, hence the need to apply organic manure to soil to enhance agricultural sustainably.

Published in	Advances in Bioscience and Bioengineering (Volume 7, Issue 2)
DOI	10.11648/j.abb.20190702.13
Page(s)	27-31
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Soil, Microorganisms, Rhizosphere, Organic Manure

References

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[14]	O’ Donnell AG, Seasman M, Macrae A, Waite I, Davies JT (2001). Plants and Fertilizers as drivers of change in microbial community structure and function in soil. Plant Soil (232): 135-45.
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Aniefon Alphonsus Ibuot, Iniobong Ime James, Mayen Godwin Ben, Christiana Utibe Etuk, Agnes Monday Jones, et al. (2019). Microbiological Analysis of Top Soil and Rhizosphere Treated with Organic Manure. Advances in Bioscience and Bioengineering, 7(2), 27-31. https://doi.org/10.11648/j.abb.20190702.13

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ACS Style

Aniefon Alphonsus Ibuot; Iniobong Ime James; Mayen Godwin Ben; Christiana Utibe Etuk; Agnes Monday Jones, et al. Microbiological Analysis of Top Soil and Rhizosphere Treated with Organic Manure. Adv. BioSci. Bioeng. 2019, 7(2), 27-31. doi: 10.11648/j.abb.20190702.13

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AMA Style

Aniefon Alphonsus Ibuot, Iniobong Ime James, Mayen Godwin Ben, Christiana Utibe Etuk, Agnes Monday Jones, et al. Microbiological Analysis of Top Soil and Rhizosphere Treated with Organic Manure. Adv BioSci Bioeng. 2019;7(2):27-31. doi: 10.11648/j.abb.20190702.13

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@article{10.11648/j.abb.20190702.13,
  author = {Aniefon Alphonsus Ibuot and Iniobong Ime James and Mayen Godwin Ben and Christiana Utibe Etuk and Agnes Monday Jones and Emmanuel Anthony Umoren and Elizabeth Lazarus Akpan and Esther Ndarake Akpan},
  title = {Microbiological Analysis of Top Soil and Rhizosphere Treated with Organic Manure},
  journal = {Advances in Bioscience and Bioengineering},
  volume = {7},
  number = {2},
  pages = {27-31},
  doi = {10.11648/j.abb.20190702.13},
  url = {https://doi.org/10.11648/j.abb.20190702.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.abb.20190702.13},
  abstract = {Microbiological analysis of topsoil and rhizosphere treated with organic manure (Poultry droppings) was carried out. Soil samples were analyzed at three days interval (Day 1, Day 4, Day 7 and Day 10). The total bacterial count recorded for rhizosphere soil treated with poultry droppings (RS:P) had the highest bacterial count ranging between 2.1×106 and 5.7×106 CFU/g. Top soil treated with poultry droppings had total bacterial count ranging between 1.9×106 and 4.9×106 CFU/g. The controls (untreated rhizosphere soil) had a total bacterial count ranging between 2.6×106 and 4.0×106 CFU/g and untreated topsoil had bacterial count ranging between 1.4×106 and 2.4×106 CFU/g. Total fungal count for top soil treated with poultry droppings ranged between 0.2×106 and 0.9×106 CFU/g. Total fungal count for rhizosphere soil treated with poultry droppings ranged between 0.2×106 and 0.3×106 CFU/g. Untreated top soil had total fungal count ranging between 0.1×106 and 0.2×106 CFU/g while untreated rhizosphere soil had a total fungal count ranging between 0.1×106 and 0.2×106 CFU/g. Bacterial isolates identified with their percentage frequency of occurrence were Bacillus sp (16.8), Enterococus sp (8.4), Clostridium sp (4.0), Staphylococcus sp (8.0) Pseudomonas sp (15.6), Listeria sp (12.0), Micrococcus sp (14.0), Serratia sp (4.8) and Streptococcus sp (7.2). Fungal isolates identified with their percentage frequency of occurrence were Rhizosphere sp (26.7%), Penicillium sp (22.5%), Aspergillus sp (21.1%), Mucor sp (19.7%) and lastly Cladosporium sp (9.8%). Metabolites secreted by the root system act as chemical signal attracting high population of microorganisms. The application of organic manure to the soil enhanced the microbial population of the soil, hence the need to apply organic manure to soil to enhance agricultural sustainably.},
 year = {2019}
}

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TY  - JOUR
T1  - Microbiological Analysis of Top Soil and Rhizosphere Treated with Organic Manure
AU  - Aniefon Alphonsus Ibuot
AU  - Iniobong Ime James
AU  - Mayen Godwin Ben
AU  - Christiana Utibe Etuk
AU  - Agnes Monday Jones
AU  - Emmanuel Anthony Umoren
AU  - Elizabeth Lazarus Akpan
AU  - Esther Ndarake Akpan
Y1  - 2019/07/26
PY  - 2019
N1  - https://doi.org/10.11648/j.abb.20190702.13
DO  - 10.11648/j.abb.20190702.13
T2  - Advances in Bioscience and Bioengineering
JF  - Advances in Bioscience and Bioengineering
JO  - Advances in Bioscience and Bioengineering
SP  - 27
EP  - 31
PB  - Science Publishing Group
SN  - 2330-4162
UR  - https://doi.org/10.11648/j.abb.20190702.13
AB  - Microbiological analysis of topsoil and rhizosphere treated with organic manure (Poultry droppings) was carried out. Soil samples were analyzed at three days interval (Day 1, Day 4, Day 7 and Day 10). The total bacterial count recorded for rhizosphere soil treated with poultry droppings (RS:P) had the highest bacterial count ranging between 2.1×106 and 5.7×106 CFU/g. Top soil treated with poultry droppings had total bacterial count ranging between 1.9×106 and 4.9×106 CFU/g. The controls (untreated rhizosphere soil) had a total bacterial count ranging between 2.6×106 and 4.0×106 CFU/g and untreated topsoil had bacterial count ranging between 1.4×106 and 2.4×106 CFU/g. Total fungal count for top soil treated with poultry droppings ranged between 0.2×106 and 0.9×106 CFU/g. Total fungal count for rhizosphere soil treated with poultry droppings ranged between 0.2×106 and 0.3×106 CFU/g. Untreated top soil had total fungal count ranging between 0.1×106 and 0.2×106 CFU/g while untreated rhizosphere soil had a total fungal count ranging between 0.1×106 and 0.2×106 CFU/g. Bacterial isolates identified with their percentage frequency of occurrence were Bacillus sp (16.8), Enterococus sp (8.4), Clostridium sp (4.0), Staphylococcus sp (8.0) Pseudomonas sp (15.6), Listeria sp (12.0), Micrococcus sp (14.0), Serratia sp (4.8) and Streptococcus sp (7.2). Fungal isolates identified with their percentage frequency of occurrence were Rhizosphere sp (26.7%), Penicillium sp (22.5%), Aspergillus sp (21.1%), Mucor sp (19.7%) and lastly Cladosporium sp (9.8%). Metabolites secreted by the root system act as chemical signal attracting high population of microorganisms. The application of organic manure to the soil enhanced the microbial population of the soil, hence the need to apply organic manure to soil to enhance agricultural sustainably.
VL  - 7
IS  - 2
ER  -

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Author Information

Aniefon Alphonsus Ibuot

Department of Science Technology, Akwa Ibom State Polytechnic, Ikot Osurua, Ikot Ekpene, Nigeria
Iniobong Ime James

Department of Science Technology, Akwa Ibom State Polytechnic, Ikot Osurua, Ikot Ekpene, Nigeria
Mayen Godwin Ben

Department of Science Technology, Akwa Ibom State Polytechnic, Ikot Osurua, Ikot Ekpene, Nigeria
Christiana Utibe Etuk

Department of Science Technology, Akwa Ibom State Polytechnic, Ikot Osurua, Ikot Ekpene, Nigeria
Agnes Monday Jones

Department of Science Technology, Akwa Ibom State Polytechnic, Ikot Osurua, Ikot Ekpene, Nigeria
Emmanuel Anthony Umoren

Department of Science Technology, Akwa Ibom State Polytechnic, Ikot Osurua, Ikot Ekpene, Nigeria
Elizabeth Lazarus Akpan

Department of Science Technology, Akwa Ibom State Polytechnic, Ikot Osurua, Ikot Ekpene, Nigeria
Esther Ndarake Akpan

Department of Science Technology, Akwa Ibom State Polytechnic, Ikot Osurua, Ikot Ekpene, Nigeria

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APA Style

Aniefon Alphonsus Ibuot, Iniobong Ime James, Mayen Godwin Ben, Christiana Utibe Etuk, Agnes Monday Jones, et al. (2019). Microbiological Analysis of Top Soil and Rhizosphere Treated with Organic Manure. Advances in Bioscience and Bioengineering, 7(2), 27-31. https://doi.org/10.11648/j.abb.20190702.13

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ACS Style

Aniefon Alphonsus Ibuot; Iniobong Ime James; Mayen Godwin Ben; Christiana Utibe Etuk; Agnes Monday Jones, et al. Microbiological Analysis of Top Soil and Rhizosphere Treated with Organic Manure. Adv. BioSci. Bioeng. 2019, 7(2), 27-31. doi: 10.11648/j.abb.20190702.13

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AMA Style

Aniefon Alphonsus Ibuot, Iniobong Ime James, Mayen Godwin Ben, Christiana Utibe Etuk, Agnes Monday Jones, et al. Microbiological Analysis of Top Soil and Rhizosphere Treated with Organic Manure. Adv BioSci Bioeng. 2019;7(2):27-31. doi: 10.11648/j.abb.20190702.13

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@article{10.11648/j.abb.20190702.13,
  author = {Aniefon Alphonsus Ibuot and Iniobong Ime James and Mayen Godwin Ben and Christiana Utibe Etuk and Agnes Monday Jones and Emmanuel Anthony Umoren and Elizabeth Lazarus Akpan and Esther Ndarake Akpan},
  title = {Microbiological Analysis of Top Soil and Rhizosphere Treated with Organic Manure},
  journal = {Advances in Bioscience and Bioengineering},
  volume = {7},
  number = {2},
  pages = {27-31},
  doi = {10.11648/j.abb.20190702.13},
  url = {https://doi.org/10.11648/j.abb.20190702.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.abb.20190702.13},
  abstract = {Microbiological analysis of topsoil and rhizosphere treated with organic manure (Poultry droppings) was carried out. Soil samples were analyzed at three days interval (Day 1, Day 4, Day 7 and Day 10). The total bacterial count recorded for rhizosphere soil treated with poultry droppings (RS:P) had the highest bacterial count ranging between 2.1×106 and 5.7×106 CFU/g. Top soil treated with poultry droppings had total bacterial count ranging between 1.9×106 and 4.9×106 CFU/g. The controls (untreated rhizosphere soil) had a total bacterial count ranging between 2.6×106 and 4.0×106 CFU/g and untreated topsoil had bacterial count ranging between 1.4×106 and 2.4×106 CFU/g. Total fungal count for top soil treated with poultry droppings ranged between 0.2×106 and 0.9×106 CFU/g. Total fungal count for rhizosphere soil treated with poultry droppings ranged between 0.2×106 and 0.3×106 CFU/g. Untreated top soil had total fungal count ranging between 0.1×106 and 0.2×106 CFU/g while untreated rhizosphere soil had a total fungal count ranging between 0.1×106 and 0.2×106 CFU/g. Bacterial isolates identified with their percentage frequency of occurrence were Bacillus sp (16.8), Enterococus sp (8.4), Clostridium sp (4.0), Staphylococcus sp (8.0) Pseudomonas sp (15.6), Listeria sp (12.0), Micrococcus sp (14.0), Serratia sp (4.8) and Streptococcus sp (7.2). Fungal isolates identified with their percentage frequency of occurrence were Rhizosphere sp (26.7%), Penicillium sp (22.5%), Aspergillus sp (21.1%), Mucor sp (19.7%) and lastly Cladosporium sp (9.8%). Metabolites secreted by the root system act as chemical signal attracting high population of microorganisms. The application of organic manure to the soil enhanced the microbial population of the soil, hence the need to apply organic manure to soil to enhance agricultural sustainably.},
 year = {2019}
}

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TY  - JOUR
T1  - Microbiological Analysis of Top Soil and Rhizosphere Treated with Organic Manure
AU  - Aniefon Alphonsus Ibuot
AU  - Iniobong Ime James
AU  - Mayen Godwin Ben
AU  - Christiana Utibe Etuk
AU  - Agnes Monday Jones
AU  - Emmanuel Anthony Umoren
AU  - Elizabeth Lazarus Akpan
AU  - Esther Ndarake Akpan
Y1  - 2019/07/26
PY  - 2019
N1  - https://doi.org/10.11648/j.abb.20190702.13
DO  - 10.11648/j.abb.20190702.13
T2  - Advances in Bioscience and Bioengineering
JF  - Advances in Bioscience and Bioengineering
JO  - Advances in Bioscience and Bioengineering
SP  - 27
EP  - 31
PB  - Science Publishing Group
SN  - 2330-4162
UR  - https://doi.org/10.11648/j.abb.20190702.13
AB  - Microbiological analysis of topsoil and rhizosphere treated with organic manure (Poultry droppings) was carried out. Soil samples were analyzed at three days interval (Day 1, Day 4, Day 7 and Day 10). The total bacterial count recorded for rhizosphere soil treated with poultry droppings (RS:P) had the highest bacterial count ranging between 2.1×106 and 5.7×106 CFU/g. Top soil treated with poultry droppings had total bacterial count ranging between 1.9×106 and 4.9×106 CFU/g. The controls (untreated rhizosphere soil) had a total bacterial count ranging between 2.6×106 and 4.0×106 CFU/g and untreated topsoil had bacterial count ranging between 1.4×106 and 2.4×106 CFU/g. Total fungal count for top soil treated with poultry droppings ranged between 0.2×106 and 0.9×106 CFU/g. Total fungal count for rhizosphere soil treated with poultry droppings ranged between 0.2×106 and 0.3×106 CFU/g. Untreated top soil had total fungal count ranging between 0.1×106 and 0.2×106 CFU/g while untreated rhizosphere soil had a total fungal count ranging between 0.1×106 and 0.2×106 CFU/g. Bacterial isolates identified with their percentage frequency of occurrence were Bacillus sp (16.8), Enterococus sp (8.4), Clostridium sp (4.0), Staphylococcus sp (8.0) Pseudomonas sp (15.6), Listeria sp (12.0), Micrococcus sp (14.0), Serratia sp (4.8) and Streptococcus sp (7.2). Fungal isolates identified with their percentage frequency of occurrence were Rhizosphere sp (26.7%), Penicillium sp (22.5%), Aspergillus sp (21.1%), Mucor sp (19.7%) and lastly Cladosporium sp (9.8%). Metabolites secreted by the root system act as chemical signal attracting high population of microorganisms. The application of organic manure to the soil enhanced the microbial population of the soil, hence the need to apply organic manure to soil to enhance agricultural sustainably.
VL  - 7
IS  - 2
ER  -

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