Journal of Diseases and Medicinal Plants

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In Vitro Therapeutic Potential of Leaf Extract of Eugenia uniflora linn on Acute – inflammation Rat Model

Received: Feb. 27, 2020    Accepted: Mar. 18, 2020    Published: May 12, 2020
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Abstract

The leaves of Eugenia uniflora are used as anti-inflammatory and analgesic in the treatment of tissue injury in traditional medicine. The study evaluates the anti-inflammatory, analgesic, hematological and histological effects of ethanol extract of E. uniflora. The anti-inflammatory effect was accessed in test groups given different doses (50, 100 and 200 mg/kg, p. o) of the extract, a control group (0.1 mL/kg, normal saline) and standard drug group (Ibuprofen 100mg/kg, p. o) on formalin- induced paw edema. Body weight, paw weight, thickness and volume were evaluated. Analgesic property was accessed on separate group of animals by acetic acid-induced writhing and formalin-induced paw licking tests. Thereafter, hematological parameters were estimated and sections of the edematous skin were processed and stained with H and E. The Eugenia uniflora decreased paw volume and thickness after 3hr. There was dose dependent significant (P<0.05) decrease in the number of abdominal writhing and insignificant reduction in the second or late phases of formalin-induced paw licking test group. Reduction in RBC, HB, PCV and increase in platelet, ESR, WBC were observed in the formalin-induced paw edema group. Histopathological assessment revealed aggregation of inflammatory cells in skin sections of the untreated group of the formalin-induced paw edema but a noticeable reversal in the extract and Ibuprofen treatment groups. These profiles indicate E. uniflora has potent anti-inflammatory and analgesic effects comparable to Ibuprofen a non steroidal anti-inflammatory drug (NSAIDs) and justifies it use in the treatment of inflammation and local tissue injury.

DOI 10.11648/j.jdmp.20200602.11
Published in Journal of Diseases and Medicinal Plants ( Volume 6, Issue 2, June 2020 )
Page(s) 31-38
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Edema, Inflammation, Analgesic, E. uniflora, Ibuprofen, Formalin

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    Elizabeth Finbarrs Bello, Abireh Ifeanacho Ezeteonu, Ude Vincent. (2020). In Vitro Therapeutic Potential of Leaf Extract of Eugenia uniflora linn on Acute – inflammation Rat Model. Journal of Diseases and Medicinal Plants, 6(2), 31-38. https://doi.org/10.11648/j.jdmp.20200602.11

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    ACS Style

    Elizabeth Finbarrs Bello; Abireh Ifeanacho Ezeteonu; Ude Vincent. In Vitro Therapeutic Potential of Leaf Extract of Eugenia uniflora linn on Acute – inflammation Rat Model. J. Dis. Med. Plants 2020, 6(2), 31-38. doi: 10.11648/j.jdmp.20200602.11

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    AMA Style

    Elizabeth Finbarrs Bello, Abireh Ifeanacho Ezeteonu, Ude Vincent. In Vitro Therapeutic Potential of Leaf Extract of Eugenia uniflora linn on Acute – inflammation Rat Model. J Dis Med Plants. 2020;6(2):31-38. doi: 10.11648/j.jdmp.20200602.11

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  • @article{10.11648/j.jdmp.20200602.11,
      author = {Elizabeth Finbarrs Bello and Abireh Ifeanacho Ezeteonu and Ude Vincent},
      title = {In Vitro Therapeutic Potential of Leaf Extract of Eugenia uniflora linn on Acute – inflammation Rat Model},
      journal = {Journal of Diseases and Medicinal Plants},
      volume = {6},
      number = {2},
      pages = {31-38},
      doi = {10.11648/j.jdmp.20200602.11},
      url = {https://doi.org/10.11648/j.jdmp.20200602.11},
      eprint = {https://download.sciencepg.com/pdf/10.11648.j.jdmp.20200602.11},
      abstract = {The leaves of Eugenia uniflora are used as anti-inflammatory and analgesic in the treatment of tissue injury in traditional medicine. The study evaluates the anti-inflammatory, analgesic, hematological and histological effects of ethanol extract of E. uniflora. The anti-inflammatory effect was accessed in test groups given different doses (50, 100 and 200 mg/kg, p. o) of the extract, a control group (0.1 mL/kg, normal saline) and standard drug group (Ibuprofen 100mg/kg, p. o) on formalin- induced paw edema. Body weight, paw weight, thickness and volume were evaluated. Analgesic property was accessed on separate group of animals by acetic acid-induced writhing and formalin-induced paw licking tests. Thereafter, hematological parameters were estimated and sections of the edematous skin were processed and stained with H and E. The Eugenia uniflora decreased paw volume and thickness after 3hr. There was dose dependent significant (PE. uniflora has potent anti-inflammatory and analgesic effects comparable to Ibuprofen a non steroidal anti-inflammatory drug (NSAIDs) and justifies it use in the treatment of inflammation and local tissue injury.},
     year = {2020}
    }
    

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    AB  - The leaves of Eugenia uniflora are used as anti-inflammatory and analgesic in the treatment of tissue injury in traditional medicine. The study evaluates the anti-inflammatory, analgesic, hematological and histological effects of ethanol extract of E. uniflora. The anti-inflammatory effect was accessed in test groups given different doses (50, 100 and 200 mg/kg, p. o) of the extract, a control group (0.1 mL/kg, normal saline) and standard drug group (Ibuprofen 100mg/kg, p. o) on formalin- induced paw edema. Body weight, paw weight, thickness and volume were evaluated. Analgesic property was accessed on separate group of animals by acetic acid-induced writhing and formalin-induced paw licking tests. Thereafter, hematological parameters were estimated and sections of the edematous skin were processed and stained with H and E. The Eugenia uniflora decreased paw volume and thickness after 3hr. There was dose dependent significant (PE. uniflora has potent anti-inflammatory and analgesic effects comparable to Ibuprofen a non steroidal anti-inflammatory drug (NSAIDs) and justifies it use in the treatment of inflammation and local tissue injury.
    VL  - 6
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Author Information
  • Department of Anatomy, Faculty of Basic Medical Sciences, Enugu State University of Science and Technology, Parklane, Enugu, Enugu State, Nigeria

  • Department of Anatomy, Faculty of Basic Medical Sciences, Enugu State University of Science and Technology, Parklane, Enugu, Enugu State, Nigeria

  • Department of Anatomy, Faculty of Basic Medical Sciences, Enugu State University of Science and Technology, Parklane, Enugu, Enugu State, Nigeria

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