Frontiers in Environmental Microbiology

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Utilization of Some Agro-Wastes for Cultivation of Pluerotus ostreatus (Oyster Mushroom) in Keffi Nigeria

Received: Apr. 09, 2019    Accepted: May 27, 2019    Published: Jul. 15, 2019
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Abstract

Commercial crop production and many other agro-industrial activities generate large amount of cellulose, hemicelluloses and lignin. This research is aimed at the utilization of some of these agro-wastes for the cultivation of Pleurotus ostreatus (Oyster Mushroom) in Keffi area. The agro-wastes (cassava peels (CP), banana leaves (BL), saw dust (SD), yam peels (YP) and groundnut shells (GS) were collected, sorted, shredded, sterilized and inoculated with equal proportions of Pleurotus ostreatus (P. osreatus) for a period of four weeks. Rate of mycelia growth, temperature, mycological identification, nutritional contents of P. osreatus harvested and microbial isolation from the spent mushroom substrates were analyzed. The numbers of days for complete ramification of the substrates by mycelia of P. osreatus were; 18 days for BL and GS, 24 days for CP and YP while 30 days for SD. The room temperature within the period of exposure for fruiting of the ramified substrates ranges between 22°C to 28°C in the morning, 23°C to 30°C in the afternoon and 25°C to 31°C in the evening throughout the period of exposure. The nutritional contents of the Pleurotus ostreatus from the different substrates analysed were, Moisture content was highest in SD (87.05%) and lest in GS (80.71%), Protein content was highest in GS (38.13%) and lowest in BL (16.43%), Lipid content was highest in GS (4.62%) and lest in CP (1.83%), Carbohydrate content was highest in CP (78.40%) and lest in GS (71.66%), Fibre content was highest in BL (9.10%) and lest in YP (8.20%) while Ash content was highest in GS (7.37%) and lest in YP (6.42%). Five bacteria species (Bacillus cereus, Pseudomonas aeruginosa, Micrococcus roseus, Escherichia coli and Bacillus subtilis) and two yeast species (Candida albicans and Candida tropicalis) were isolated from the spent mushroom substrates.

DOI 10.11648/j.fem.20190502.13
Published in Frontiers in Environmental Microbiology ( Volume 5, Issue 2, April 2019 )
Page(s) 60-69
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Cellulose, Hemicellulose, Lignin, Agro-waste, Pleurotus ostreatus, Inoculated, Mycelia, Mycological

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    Odonye Enoch Peter, Galleh Raphael Peter, Ishaya Isa Obele, Gabriel Owuna, Makut Makwin Danladi, et al. (2019). Utilization of Some Agro-Wastes for Cultivation of Pluerotus ostreatus (Oyster Mushroom) in Keffi Nigeria. Frontiers in Environmental Microbiology, 5(2), 60-69. https://doi.org/10.11648/j.fem.20190502.13

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    Odonye Enoch Peter; Galleh Raphael Peter; Ishaya Isa Obele; Gabriel Owuna; Makut Makwin Danladi, et al. Utilization of Some Agro-Wastes for Cultivation of Pluerotus ostreatus (Oyster Mushroom) in Keffi Nigeria. Front. Environ. Microbiol. 2019, 5(2), 60-69. doi: 10.11648/j.fem.20190502.13

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    AMA Style

    Odonye Enoch Peter, Galleh Raphael Peter, Ishaya Isa Obele, Gabriel Owuna, Makut Makwin Danladi, et al. Utilization of Some Agro-Wastes for Cultivation of Pluerotus ostreatus (Oyster Mushroom) in Keffi Nigeria. Front Environ Microbiol. 2019;5(2):60-69. doi: 10.11648/j.fem.20190502.13

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  • @article{10.11648/j.fem.20190502.13,
      author = {Odonye Enoch Peter and Galleh Raphael Peter and Ishaya Isa Obele and Gabriel Owuna and Makut Makwin Danladi and Smart Obiekieze and Ombugadu Akwashiki},
      title = {Utilization of Some Agro-Wastes for Cultivation of Pluerotus ostreatus (Oyster Mushroom) in Keffi Nigeria},
      journal = {Frontiers in Environmental Microbiology},
      volume = {5},
      number = {2},
      pages = {60-69},
      doi = {10.11648/j.fem.20190502.13},
      url = {https://doi.org/10.11648/j.fem.20190502.13},
      eprint = {https://download.sciencepg.com/pdf/10.11648.j.fem.20190502.13},
      abstract = {Commercial crop production and many other agro-industrial activities generate large amount of cellulose, hemicelluloses and lignin. This research is aimed at the utilization of some of these agro-wastes for the cultivation of Pleurotus ostreatus (Oyster Mushroom) in Keffi area. The agro-wastes (cassava peels (CP), banana leaves (BL), saw dust (SD), yam peels (YP) and groundnut shells (GS) were collected, sorted, shredded, sterilized and inoculated with equal proportions of Pleurotus ostreatus (P. osreatus) for a period of four weeks. Rate of mycelia growth, temperature, mycological identification, nutritional contents of P. osreatus harvested and microbial isolation from the spent mushroom substrates were analyzed. The numbers of days for complete ramification of the substrates by mycelia of P. osreatus were; 18 days for BL and GS, 24 days for CP and YP while 30 days for SD. The room temperature within the period of exposure for fruiting of the ramified substrates ranges between 22°C to 28°C in the morning, 23°C to 30°C in the afternoon and 25°C to 31°C in the evening throughout the period of exposure. The nutritional contents of the Pleurotus ostreatus from the different substrates analysed were, Moisture content was highest in SD (87.05%) and lest in GS (80.71%), Protein content was highest in GS (38.13%) and lowest in BL (16.43%), Lipid content was highest in GS (4.62%) and lest in CP (1.83%), Carbohydrate content was highest in CP (78.40%) and lest in GS (71.66%), Fibre content was highest in BL (9.10%) and lest in YP (8.20%) while Ash content was highest in GS (7.37%) and lest in YP (6.42%). Five bacteria species (Bacillus cereus, Pseudomonas aeruginosa, Micrococcus roseus, Escherichia coli and Bacillus subtilis) and two yeast species (Candida albicans and Candida tropicalis) were isolated from the spent mushroom substrates.},
     year = {2019}
    }
    

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  • TY  - JOUR
    T1  - Utilization of Some Agro-Wastes for Cultivation of Pluerotus ostreatus (Oyster Mushroom) in Keffi Nigeria
    AU  - Odonye Enoch Peter
    AU  - Galleh Raphael Peter
    AU  - Ishaya Isa Obele
    AU  - Gabriel Owuna
    AU  - Makut Makwin Danladi
    AU  - Smart Obiekieze
    AU  - Ombugadu Akwashiki
    Y1  - 2019/07/15
    PY  - 2019
    N1  - https://doi.org/10.11648/j.fem.20190502.13
    DO  - 10.11648/j.fem.20190502.13
    T2  - Frontiers in Environmental Microbiology
    JF  - Frontiers in Environmental Microbiology
    JO  - Frontiers in Environmental Microbiology
    SP  - 60
    EP  - 69
    PB  - Science Publishing Group
    SN  - 2469-8067
    UR  - https://doi.org/10.11648/j.fem.20190502.13
    AB  - Commercial crop production and many other agro-industrial activities generate large amount of cellulose, hemicelluloses and lignin. This research is aimed at the utilization of some of these agro-wastes for the cultivation of Pleurotus ostreatus (Oyster Mushroom) in Keffi area. The agro-wastes (cassava peels (CP), banana leaves (BL), saw dust (SD), yam peels (YP) and groundnut shells (GS) were collected, sorted, shredded, sterilized and inoculated with equal proportions of Pleurotus ostreatus (P. osreatus) for a period of four weeks. Rate of mycelia growth, temperature, mycological identification, nutritional contents of P. osreatus harvested and microbial isolation from the spent mushroom substrates were analyzed. The numbers of days for complete ramification of the substrates by mycelia of P. osreatus were; 18 days for BL and GS, 24 days for CP and YP while 30 days for SD. The room temperature within the period of exposure for fruiting of the ramified substrates ranges between 22°C to 28°C in the morning, 23°C to 30°C in the afternoon and 25°C to 31°C in the evening throughout the period of exposure. The nutritional contents of the Pleurotus ostreatus from the different substrates analysed were, Moisture content was highest in SD (87.05%) and lest in GS (80.71%), Protein content was highest in GS (38.13%) and lowest in BL (16.43%), Lipid content was highest in GS (4.62%) and lest in CP (1.83%), Carbohydrate content was highest in CP (78.40%) and lest in GS (71.66%), Fibre content was highest in BL (9.10%) and lest in YP (8.20%) while Ash content was highest in GS (7.37%) and lest in YP (6.42%). Five bacteria species (Bacillus cereus, Pseudomonas aeruginosa, Micrococcus roseus, Escherichia coli and Bacillus subtilis) and two yeast species (Candida albicans and Candida tropicalis) were isolated from the spent mushroom substrates.
    VL  - 5
    IS  - 2
    ER  - 

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Author Information
  • Department of Microbiology, Faculty of Natural and Applied Sciences, Nasarawa State University, Keffi, Nigeria

  • Department of Microbiology, Faculty of Natural and Applied Sciences, Nasarawa State University, Keffi, Nigeria

  • Department of Microbiology, Faculty of Natural and Applied Sciences, Nasarawa State University, Keffi, Nigeria

  • Department of Microbiology, Faculty of Natural and Applied Sciences, Nasarawa State University, Keffi, Nigeria

  • Department of Microbiology, Faculty of Natural and Applied Sciences, Nasarawa State University, Keffi, Nigeria

  • Department of Microbiology, Faculty of Natural and Applied Sciences, Nasarawa State University, Keffi, Nigeria

  • Department of Zoology, Faculty of Science, Federal University Lafia, Nasarawa, Nigeria

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