This study was designed to screen microorganisms from decomposing palm kernel shaft for cellulase palm oil processing sites in Akoko area of Ondo State, Nigeria. Isolation of microorganisms was carried out by serial dilution and pour plate methods andidentified using standard biochemical methods. The isolates were screened for cellulase production using standard assay methods. The microorganisms were grown in a mineral salt basal medium for maximum yield of cellulase production. The microorganisms isolated from the sample include Bacillus subtilis, Micrococcus varians, M. leteus, Cellulomonas blazotes, C. flavigina, Sarcina ventriculi, B. cereus, C. fimi, Aspergillus niger, Rhizopus stolonifer, Saccharomyces cerevisiae and Trichoderma viridae. The screened microorganisms exhibited varied cellulase activities. The diameters of zones of clearance of the isolates ranged from 1.45 to 1.83for bacteria and 0.00 to 2.06for fungi. The cellulase activity exhibited by bacteria ranged from 0.238 µmol/ml to 0.590 µmol/ml while fungal cellulase activity ranged from 0.452 µmol/ml to 0.775 µmol/ml. The high cellulase activity exhibited by fungi isolated from decomposing palm kernel shaft suggested that their predominance as a potential source of cellulase could be more promising in various industrial processes for the degradation of cellulose containing organic substances.
| Published in | Frontiers in Environmental Microbiology (Volume 5, Issue 5) |
| DOI | 10.11648/j.fem.20190505.11 |
| Page(s) | 100-105 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2024. Published by Science Publishing Group |
Cellulose, Wastes, Palm Kernel Shaft, Cellulose, Microorganisms
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APA Style
Adegbehingbe Kehinde Tope, Bello Marcus Oluyemi, Fakoya Soji, Adeleke Bartholomew Saanu, Jemilaiye Taiye Anangwureyi, et al. (2020). Isolation and Screening of Microbial Isolates from Decomposing Palm Kernel Shaft for Cellulase Production. Frontiers in Environmental Microbiology, 5(5), 100-105. https://doi.org/10.11648/j.fem.20190505.11
ACS Style
Adegbehingbe Kehinde Tope; Bello Marcus Oluyemi; Fakoya Soji; Adeleke Bartholomew Saanu; Jemilaiye Taiye Anangwureyi, et al. Isolation and Screening of Microbial Isolates from Decomposing Palm Kernel Shaft for Cellulase Production. Front. Environ. Microbiol. 2020, 5(5), 100-105. doi: 10.11648/j.fem.20190505.11
AMA Style
Adegbehingbe Kehinde Tope, Bello Marcus Oluyemi, Fakoya Soji, Adeleke Bartholomew Saanu, Jemilaiye Taiye Anangwureyi, et al. Isolation and Screening of Microbial Isolates from Decomposing Palm Kernel Shaft for Cellulase Production. Front Environ Microbiol. 2020;5(5):100-105. doi: 10.11648/j.fem.20190505.11
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Download@article{10.11648/j.fem.20190505.11,
author = {Adegbehingbe Kehinde Tope and Bello Marcus Oluyemi and Fakoya Soji and Adeleke Bartholomew Saanu and Jemilaiye Taiye Anangwureyi and Orege Samuel Temidire},
title = {Isolation and Screening of Microbial Isolates from Decomposing Palm Kernel Shaft for Cellulase Production},
journal = {Frontiers in Environmental Microbiology},
volume = {5},
number = {5},
pages = {100-105},
doi = {10.11648/j.fem.20190505.11},
url = {https://doi.org/10.11648/j.fem.20190505.11},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.fem.20190505.11},
abstract = {This study was designed to screen microorganisms from decomposing palm kernel shaft for cellulase palm oil processing sites in Akoko area of Ondo State, Nigeria. Isolation of microorganisms was carried out by serial dilution and pour plate methods andidentified using standard biochemical methods. The isolates were screened for cellulase production using standard assay methods. The microorganisms were grown in a mineral salt basal medium for maximum yield of cellulase production. The microorganisms isolated from the sample include Bacillus subtilis, Micrococcus varians, M. leteus, Cellulomonas blazotes, C. flavigina, Sarcina ventriculi, B. cereus, C. fimi, Aspergillus niger, Rhizopus stolonifer, Saccharomyces cerevisiae and Trichoderma viridae. The screened microorganisms exhibited varied cellulase activities. The diameters of zones of clearance of the isolates ranged from 1.45 to 1.83for bacteria and 0.00 to 2.06for fungi. The cellulase activity exhibited by bacteria ranged from 0.238 µmol/ml to 0.590 µmol/ml while fungal cellulase activity ranged from 0.452 µmol/ml to 0.775 µmol/ml. The high cellulase activity exhibited by fungi isolated from decomposing palm kernel shaft suggested that their predominance as a potential source of cellulase could be more promising in various industrial processes for the degradation of cellulose containing organic substances.},
year = {2020}
}
TY - JOUR T1 - Isolation and Screening of Microbial Isolates from Decomposing Palm Kernel Shaft for Cellulase Production AU - Adegbehingbe Kehinde Tope AU - Bello Marcus Oluyemi AU - Fakoya Soji AU - Adeleke Bartholomew Saanu AU - Jemilaiye Taiye Anangwureyi AU - Orege Samuel Temidire Y1 - 2020/01/06 PY - 2020 N1 - https://doi.org/10.11648/j.fem.20190505.11 DO - 10.11648/j.fem.20190505.11 T2 - Frontiers in Environmental Microbiology JF - Frontiers in Environmental Microbiology JO - Frontiers in Environmental Microbiology SP - 100 EP - 105 PB - Science Publishing Group SN - 2469-8067 UR - https://doi.org/10.11648/j.fem.20190505.11 AB - This study was designed to screen microorganisms from decomposing palm kernel shaft for cellulase palm oil processing sites in Akoko area of Ondo State, Nigeria. Isolation of microorganisms was carried out by serial dilution and pour plate methods andidentified using standard biochemical methods. The isolates were screened for cellulase production using standard assay methods. The microorganisms were grown in a mineral salt basal medium for maximum yield of cellulase production. The microorganisms isolated from the sample include Bacillus subtilis, Micrococcus varians, M. leteus, Cellulomonas blazotes, C. flavigina, Sarcina ventriculi, B. cereus, C. fimi, Aspergillus niger, Rhizopus stolonifer, Saccharomyces cerevisiae and Trichoderma viridae. The screened microorganisms exhibited varied cellulase activities. The diameters of zones of clearance of the isolates ranged from 1.45 to 1.83for bacteria and 0.00 to 2.06for fungi. The cellulase activity exhibited by bacteria ranged from 0.238 µmol/ml to 0.590 µmol/ml while fungal cellulase activity ranged from 0.452 µmol/ml to 0.775 µmol/ml. The high cellulase activity exhibited by fungi isolated from decomposing palm kernel shaft suggested that their predominance as a potential source of cellulase could be more promising in various industrial processes for the degradation of cellulose containing organic substances. VL - 5 IS - 5 ER -
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