Molecular Screening for Potential Pathogenic Organisms in Some Six Major Representative Sources of Water in Saki

Adeoti Olatunde Micheal; Akinbo Kehinde Oluwabusayo; Adesina David Ademola; Olufemi Samson Olutope

doi:doi:10.11648/j.fem.20200601.12

| Peer-Reviewed |

Molecular Screening for Potential Pathogenic Organisms in Some Six Major Representative Sources of Water in Saki

Adeoti Olatunde Micheal, Akinbo Kehinde Oluwabusayo, Adesina David Ademola, Olufemi Samson Olutope

Received: 23 December 2019 Accepted: 16 January 2020 Published: 8 April 2020

Views: Downloads:

Download PDF

Share This Article

Twitter
Linked In
Facebook

Abstract

Water security and safety is of vital concern in Saki and the rest of the world. The aim of this was to screen major sources of water in Saki of potential pathogens of public health importance. Water samples of six different water sources were purposely collected aseptically from six different locations in Saki (well water, river water, rain water, borehole water, sachet water and bottled water); these were subjectsed to standard laboratory analysis. Four highly populated plates were sent for molecular characterization for Polymerase Chain Reaction and species sequencing for molecular identification. The highest total colony count was 70.0×10^-5 from river water followed by rain water with the lowest of 0.1×10⁵ CFU from borehole water. Molecular analysis identified amplification of isolates genes at 15000 base pair for river water and well water samples. The analysis showed that Klebsiella pneumonia (MN208186) and Enterobacter kobeii (KM593928) were the organisms found in rain water and river water respectively. The resulting nucleotide sequences was blasted at NCBI for pairwise comparison with existing data in the GenBank and their similarity was noted. The pathogenicity of the two isolates have been well-documented, making some of the water sources tested so unfit for human usage. Large-scale treatment of water sources are recommended before consumption viz-a-vis hygienic practices around water bodies.

DOI	10.11648/j.fem.20200601.12
Published in	Frontiers in Environmental Microbiology (Volume 6, Issue 1, March 2020)
Page(s)	5-10
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Fecal Coliform, Aseptically, Blasted, Enterobacter Kobeii, Water-borne Diseases, Well Water

References

[1]	Julian, T. R., MacDonald, L. H., GUO, Y., Marks, S. J., Kosek, M., Yori, P. P., Pinedo, S. R., Schwab, K. J. (2013). Fecal indicator bacterial contamination of fomites and Household demand for surface disinfection products: A case study from peru. The American journal of tropical medicine and hygiene 89: 869-872.
[2]	Lay, B., & Miter b. (2010). Emtrcolities epidemic as a result of contamination of drinking Water through E. colisterotype abstract 2b Pp 42.
[3]	Diosi, A. L., and mitard. (2011). E. coli type soil and OSS in drinking Water abstract 7b 1.
[4]	Dondero, W. C. (2009) Advanced Applied Microbiology Vol. 3 Academic press, New York London. Pp 77-178.
[5]	Gaecdrich, E. E. (2012). Waterborne pathogen in water pollution microbiology R. Mitchell, John wiley and sons inc. New York Pp 102.
[6]	Gopinath, A., pratapChandran, R., Vysakhi, MV., Anu, A., (2006). Physical and bacteriological quality Of well water samples from kanakkaypanchayath, kottayam district, Kerala State, India International Journal of Plant and Animal environ 2012.
[7]	Pelezer, M. J., R. G. D & R. D. (2012). Microbiology (3^rd edition) M. C Grawhill book company Pp 143-767.
[8]	Shibata, T. Solo-Gabriele, H. M. Fleming, BLE, Elmir S. (2004) Monitoring marine recreational water quality using multiple microbial indicators in a urban tropical environment. Water Res 38: 3119-31.
[9]	Callaway, A., Kostrzewa, M., Willershausen, B., Schmidt, F., Thiede, B., Kupper, H., &Kneist, S. (2013). Clin. Lab. 2013. 121225.
[10]	Carpenter beth, V. J. (2007). Indicator of recreational water quality American Society for Testing and Mathials.
[11]	Kelman, A., Person, L. H., and Herbert, T. T. (2007). Plant disease conference report Pp 802.
[12]	Bonde, S. J. (2009). Advances in agnatic microbiology vol. 1 London and New York Academic press.
[13]	Suthar, S. Chhimpa, V. Singh, S. (2009) Bacterial contamination in drinking water: A case study in rural areas of Northern Rajasthan, India. Environ Monitoring & Assessment 159: 43-50.
[14]	Field, D. Wills, C. (1998). Abundant microsatellite polymorphism in saccharomyces in eight prokaryotes and S. Cerevisiae, result from strong mutation pressures and variety of selective force Process. National Acadamic of. Science U. S. A. 95: 1647-1652.
[15]	WHO. (2011). Guideline for drinking water quality 3^rd edition. World Health Organization.
[16]	Ahmod, N. Z., Gupta, R. S., & Shah, H. N. (2011). J. M microbiology. Methods 87, 278-285 doi: 10. /j. mimet 2011. 08. 015.
[17]	WHO, U. (2000). Global water supply and sanitation assessment 2000 report. Geneva: World Health Organization.
[18]	WHO, U. (2014) Progress in drinking water and Sanitation: 2014 update. World.
[19]	Nuria, M. C., Rosyid, A., Sumantri, S. (2009). UjikandunganbakteriEscherichia colipada air minumisiulangdari depot air miniumisiulang di kabupatenrembang testing of Escherichia coli bacteria content drinking water refill from drinking water refill depot in Rembang sub-district. MEDIAGRO 5.
[20]	Boria, A. &Mitarb [2009]. Inter-countries epidemic as a result of contamination of drinking water with E. coli strain 0124. Abstract 2bi. Pp. 178-180.
[21]	Kahinda, J. M., A. E Taigbenua, E. S. B Lilieb and Boroto, R. J. (2009). GIS- based decision Support system for rain water harvesting (RHAESS). Physics and Chemistry of the Earth parts.

Cite This Article

Plain Text BibTeX RIS

APA Style

Adeoti Olatunde Micheal, Akinbo Kehinde Oluwabusayo, Adesina David Ademola, Olufemi Samson Olutope. (2020). Molecular Screening for Potential Pathogenic Organisms in Some Six Major Representative Sources of Water in Saki. Frontiers in Environmental Microbiology, 6(1), 5-10. https://doi.org/10.11648/j.fem.20200601.12

Copy | Download

ACS Style

Adeoti Olatunde Micheal; Akinbo Kehinde Oluwabusayo; Adesina David Ademola; Olufemi Samson Olutope. Molecular Screening for Potential Pathogenic Organisms in Some Six Major Representative Sources of Water in Saki. Front. Environ. Microbiol. 2020, 6(1), 5-10. doi: 10.11648/j.fem.20200601.12

Copy | Download

AMA Style

Adeoti Olatunde Micheal, Akinbo Kehinde Oluwabusayo, Adesina David Ademola, Olufemi Samson Olutope. Molecular Screening for Potential Pathogenic Organisms in Some Six Major Representative Sources of Water in Saki. Front Environ Microbiol. 2020;6(1):5-10. doi: 10.11648/j.fem.20200601.12

Copy | Download

@article{10.11648/j.fem.20200601.12,
  author = {Adeoti Olatunde Micheal and Akinbo Kehinde Oluwabusayo and Adesina David Ademola and Olufemi Samson Olutope},
  title = {Molecular Screening for Potential Pathogenic Organisms in Some Six Major Representative Sources of Water in Saki},
  journal = {Frontiers in Environmental Microbiology},
  volume = {6},
  number = {1},
  pages = {5-10},
  doi = {10.11648/j.fem.20200601.12},
  url = {https://doi.org/10.11648/j.fem.20200601.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.fem.20200601.12},
  abstract = {Water security and safety is of vital concern in Saki and the rest of the world. The aim of this was to screen major sources of water in Saki of potential pathogens of public health importance. Water samples of six different water sources were purposely collected aseptically from six different locations in Saki (well water, river water, rain water, borehole water, sachet water and bottled water); these were subjectsed to standard laboratory analysis. Four highly populated plates were sent for molecular characterization for Polymerase Chain Reaction and species sequencing for molecular identification. The highest total colony count was 70.0×10-5 from river water followed by rain water with the lowest of 0.1×105 CFU from borehole water. Molecular analysis identified amplification of isolates genes at 15000 base pair for river water and well water samples. The analysis showed that Klebsiella pneumonia (MN208186) and Enterobacter kobeii (KM593928) were the organisms found in rain water and river water respectively. The resulting nucleotide sequences was blasted at NCBI for pairwise comparison with existing data in the GenBank and their similarity was noted. The pathogenicity of the two isolates have been well-documented, making some of the water sources tested so unfit for human usage. Large-scale treatment of water sources are recommended before consumption viz-a-vis hygienic practices around water bodies.},
 year = {2020}
}

Copy | Download

TY  - JOUR
T1  - Molecular Screening for Potential Pathogenic Organisms in Some Six Major Representative Sources of Water in Saki
AU  - Adeoti Olatunde Micheal
AU  - Akinbo Kehinde Oluwabusayo
AU  - Adesina David Ademola
AU  - Olufemi Samson Olutope
Y1  - 2020/04/08
PY  - 2020
N1  - https://doi.org/10.11648/j.fem.20200601.12
DO  - 10.11648/j.fem.20200601.12
T2  - Frontiers in Environmental Microbiology
JF  - Frontiers in Environmental Microbiology
JO  - Frontiers in Environmental Microbiology
SP  - 5
EP  - 10
PB  - Science Publishing Group
SN  - 2469-8067
UR  - https://doi.org/10.11648/j.fem.20200601.12
AB  - Water security and safety is of vital concern in Saki and the rest of the world. The aim of this was to screen major sources of water in Saki of potential pathogens of public health importance. Water samples of six different water sources were purposely collected aseptically from six different locations in Saki (well water, river water, rain water, borehole water, sachet water and bottled water); these were subjectsed to standard laboratory analysis. Four highly populated plates were sent for molecular characterization for Polymerase Chain Reaction and species sequencing for molecular identification. The highest total colony count was 70.0×10-5 from river water followed by rain water with the lowest of 0.1×105 CFU from borehole water. Molecular analysis identified amplification of isolates genes at 15000 base pair for river water and well water samples. The analysis showed that Klebsiella pneumonia (MN208186) and Enterobacter kobeii (KM593928) were the organisms found in rain water and river water respectively. The resulting nucleotide sequences was blasted at NCBI for pairwise comparison with existing data in the GenBank and their similarity was noted. The pathogenicity of the two isolates have been well-documented, making some of the water sources tested so unfit for human usage. Large-scale treatment of water sources are recommended before consumption viz-a-vis hygienic practices around water bodies.
VL  - 6
IS  - 1
ER  -

Copy | Download

Author Information

Adeoti Olatunde Micheal

Department of Science Laboratory Technology (Microbiology Option), The Oke Ogun Polytechnic Saki, Saki, Oyo State, Nigeria
Akinbo Kehinde Oluwabusayo

Department of Science Laboratory Technology (Microbiology Option), The Oke Ogun Polytechnic Saki, Saki, Oyo State, Nigeria
Adesina David Ademola

Department of Science Laboratory Technology (Microbiology Option), The Oke Ogun Polytechnic Saki, Saki, Oyo State, Nigeria
Olufemi Samson Olutope

Department of Science Laboratory Technology (Microbiology Option), The Oke Ogun Polytechnic Saki, Saki, Oyo State, Nigeria

Download PDF

Sections

Plain Text BibTeX RIS

APA Style

Adeoti Olatunde Micheal, Akinbo Kehinde Oluwabusayo, Adesina David Ademola, Olufemi Samson Olutope. (2020). Molecular Screening for Potential Pathogenic Organisms in Some Six Major Representative Sources of Water in Saki. Frontiers in Environmental Microbiology, 6(1), 5-10. https://doi.org/10.11648/j.fem.20200601.12

Copy | Download

ACS Style

Adeoti Olatunde Micheal; Akinbo Kehinde Oluwabusayo; Adesina David Ademola; Olufemi Samson Olutope. Molecular Screening for Potential Pathogenic Organisms in Some Six Major Representative Sources of Water in Saki. Front. Environ. Microbiol. 2020, 6(1), 5-10. doi: 10.11648/j.fem.20200601.12

Copy | Download

AMA Style

Adeoti Olatunde Micheal, Akinbo Kehinde Oluwabusayo, Adesina David Ademola, Olufemi Samson Olutope. Molecular Screening for Potential Pathogenic Organisms in Some Six Major Representative Sources of Water in Saki. Front Environ Microbiol. 2020;6(1):5-10. doi: 10.11648/j.fem.20200601.12

Copy | Download

@article{10.11648/j.fem.20200601.12,
  author = {Adeoti Olatunde Micheal and Akinbo Kehinde Oluwabusayo and Adesina David Ademola and Olufemi Samson Olutope},
  title = {Molecular Screening for Potential Pathogenic Organisms in Some Six Major Representative Sources of Water in Saki},
  journal = {Frontiers in Environmental Microbiology},
  volume = {6},
  number = {1},
  pages = {5-10},
  doi = {10.11648/j.fem.20200601.12},
  url = {https://doi.org/10.11648/j.fem.20200601.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.fem.20200601.12},
  abstract = {Water security and safety is of vital concern in Saki and the rest of the world. The aim of this was to screen major sources of water in Saki of potential pathogens of public health importance. Water samples of six different water sources were purposely collected aseptically from six different locations in Saki (well water, river water, rain water, borehole water, sachet water and bottled water); these were subjectsed to standard laboratory analysis. Four highly populated plates were sent for molecular characterization for Polymerase Chain Reaction and species sequencing for molecular identification. The highest total colony count was 70.0×10-5 from river water followed by rain water with the lowest of 0.1×105 CFU from borehole water. Molecular analysis identified amplification of isolates genes at 15000 base pair for river water and well water samples. The analysis showed that Klebsiella pneumonia (MN208186) and Enterobacter kobeii (KM593928) were the organisms found in rain water and river water respectively. The resulting nucleotide sequences was blasted at NCBI for pairwise comparison with existing data in the GenBank and their similarity was noted. The pathogenicity of the two isolates have been well-documented, making some of the water sources tested so unfit for human usage. Large-scale treatment of water sources are recommended before consumption viz-a-vis hygienic practices around water bodies.},
 year = {2020}
}

Copy | Download

TY  - JOUR
T1  - Molecular Screening for Potential Pathogenic Organisms in Some Six Major Representative Sources of Water in Saki
AU  - Adeoti Olatunde Micheal
AU  - Akinbo Kehinde Oluwabusayo
AU  - Adesina David Ademola
AU  - Olufemi Samson Olutope
Y1  - 2020/04/08
PY  - 2020
N1  - https://doi.org/10.11648/j.fem.20200601.12
DO  - 10.11648/j.fem.20200601.12
T2  - Frontiers in Environmental Microbiology
JF  - Frontiers in Environmental Microbiology
JO  - Frontiers in Environmental Microbiology
SP  - 5
EP  - 10
PB  - Science Publishing Group
SN  - 2469-8067
UR  - https://doi.org/10.11648/j.fem.20200601.12
AB  - Water security and safety is of vital concern in Saki and the rest of the world. The aim of this was to screen major sources of water in Saki of potential pathogens of public health importance. Water samples of six different water sources were purposely collected aseptically from six different locations in Saki (well water, river water, rain water, borehole water, sachet water and bottled water); these were subjectsed to standard laboratory analysis. Four highly populated plates were sent for molecular characterization for Polymerase Chain Reaction and species sequencing for molecular identification. The highest total colony count was 70.0×10-5 from river water followed by rain water with the lowest of 0.1×105 CFU from borehole water. Molecular analysis identified amplification of isolates genes at 15000 base pair for river water and well water samples. The analysis showed that Klebsiella pneumonia (MN208186) and Enterobacter kobeii (KM593928) were the organisms found in rain water and river water respectively. The resulting nucleotide sequences was blasted at NCBI for pairwise comparison with existing data in the GenBank and their similarity was noted. The pathogenicity of the two isolates have been well-documented, making some of the water sources tested so unfit for human usage. Large-scale treatment of water sources are recommended before consumption viz-a-vis hygienic practices around water bodies.
VL  - 6
IS  - 1
ER  -

Copy | Download