International Journal of Food Science and Biotechnology

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Estimation of Heavy Metals, Essential Trace Elements and Anti-Nutritional Factors in Leaves and Stems from Moringa oleifera

Received: May 25, 2019    Accepted: Jun. 27, 2019    Published: Jul. 24, 2019
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Abstract

This study was conducted to estimate the concentrations of 8 trace elements including (Mn, Fe, Cu, Zn, Ni, Ar, Cr, and Pb) and 4 anti-nutritional factors including (saponins, oxalates, phytate, and cyanogenic glycosides) in leaves and stems from Moringa oleifera. The concentrations in samples analyzed were found to be in the range of 0.88-1.88 mg/kg for Mn, 11.95-25.25 mg/kg for Fe, 0.35-1.22 mg/kg for Cu, 6.92-17.96 mg/kg for Zn, 0.03-0.07 mg/kg for Ni, 0.955-1.45 mg/kg for Cr and 0.564-0.85 mg/kg for Pb. However, Arsenic was not detected in all the samples analyzed. As for the anti-nutritional factors, the concentrations in samples analyzed were found to be in the range of 111.35-123.42 mg/kg for saponins, 69.5-509.4 mg/kg for oxalates, and 0.38-0.156 mg/kg for phytate and 316.95-325.27 mg/kg for cyanogenic glycosides. The values of all these elements were found significantly below the recommended maximum tolerable guidelines level proposed by WHO/FAO except for lead (Pb), Pb was found slightly higher than the recommended limit as described. Our findings of this study reveal that most of the trace elements found in M. oleifera are below the recommended maximum tolerable limits; therefore it is safe for both human and animal consumption as well.

DOI 10.11648/j.ijfsb.20190402.14
Published in International Journal of Food Science and Biotechnology ( Volume 4, Issue 2, June 2019 )
Page(s) 51-55
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Moringa oleifera, Heavy Metals, Trace Elements and Anti-nutritional Factors

References
[1] Liu, Jinhu, Liang Cao, and Shuozeng Dou. "Bioaccumulation of heavy metals and health risk assessment in three benthic bivalves along the coast of Laizhou Bay, China." Marine pollution bulletin 117, no. 1-2 (2017): 98-110.
[2] Chen, Lian, Shenglu Zhou, Yaxing Shi, Chunhui Wang, Baojie Li, Yan Li, and Shaohua Wu. "Heavy metals in food crops, soil, and water in the Lihe River Watershed of the Taihu Region and their potential health risks when ingested." Science of the Total Environment 615 (2018): 141-149.
[3] Al-Thagafi, Z., H. Arida, and R. Hassan. "Trace toxic metal levels in canned and fresh food: a comparative study." Int J Innov Res SciEngTechnol 3, no. 2 (2014): 8977-8989.
[4] Khan, S., Q. Cao, Y. M. Zheng, Y. Z. Huang, and Y. G. Zhu. "Health risks of heavy metals in contaminated soils and food crops irrigated with wastewater in Beijing, China." Environmental pollution 152, no. 3 (2008): 686-692.
[5] Massadeh, Adnan M., Ayat Allah, and T. Al-Massaedh. "Determination of heavy metals in canned fruits and vegetables sold in Jordan market." Environmental Science and Pollution Research 25, no. 2 (2018): 1914-1920.
[6] Sulyman, Y. I., S. Abdulrazak, Y. A. Oniwapele, and A. Amad. "Concentration of heavy metals in some selected cereals sourced within Kaduna state, Nigeria." J Environ SciToxicol Food Technol 9, no. 10 (2015): 17-19.
[7] Salihu, Simon Olonkwoh, John Olusanya Jacob, Matthew TikpangiKolo, Bukola Joseph Osundiran, and John Emmanuel. "Heavy metals in some fruits and cereals in Minna markets, Nigeria." Pakistan Journal of Nutrition 13, no. 12 (2014): 722.
[8] Adesemoye, A. O., B. O. Opere, and S. C. O. Makinde. "Microbial content of abattoir wastewater and its contaminated soil in Lagos, Nigeria." African Journal of Biotechnology 5, no. 20 (2006).
[9] Fadeyibi, A., A. Halilu, and Z. D. Osunde. "Analysis of physicochemical properties of abattoir wastewater for irrigation in Minna, Niger State." Continental Journal of Water, Air and Soil Pollution 2, no. 2 (2011): 12-15.
[10] Khan, Sajjad, Robina Farooq, ShaguftaShahbaz, Mohammad Aziz Khan, and Maria Sadique. "Health risk assessment of heavy metals for population via consumption of vegetables." World ApplSci J 6, no. 12 (2009): 1602-1606.
[11] Leone, Alessandro, Alberto Spada, Alberto Battezzati, Alberto Schiraldi, Junior Aristil, and SimonaBertoli. "Cultivation, genetic, ethnopharmacology, phytochemistry and pharmacology of Moringaoleifera leaves: an overview." International journal of molecular sciences 16, no. 6 (2015): 12791-12835.
[12] Padayachee, Berushka, and HimansuBaijnath. "An overview of the medicinal importance of Moringaceae." Journal of Medicinal Plants Research 6, no. 48 (2012): 5831-5839.
[13] Anwar, Farooq, SajidLatif, Muhammad Ashraf, and Anwarul Hassan Gilani. "Moringaoleifera: a food plant with multiple medicinal uses." Phytotherapy Research: An International Journal Devoted to Pharmacological and Toxicological Evaluation of Natural Product Derivatives 21, no. 1 (2007): 17-25.
[14] Stohs, Sidney J., and Michael J. Hartman. "Review of the safety and efficacy of Moringaoleifera." Phytotherapy Research 29, no. 6 (2015): 796-804.
[15] Popoola, Jacob O., and Olawole O. Obembe. "Local knowledge, use pattern and geographical distribution of Moringa oleifera Lam.(Moringaceae) in Nigeria." Journal of Ethnopharmacology 150, no. 2 (2013): 682-691.
[16] Sivasankari, Balayogan, MarimuthuAnandharaj, and PerumalGunasekaran. "An ethnobotanical study of indigenous knowledge on medicinal plants used by the village peoples of Thoppampatti, Dindigul district, Tamilnadu, India." Journal of ethnopharmacology 153, no. 2 (2014): 408-423.
[17] Vergara-Jimenez, Marcela, Manal Almatrafi, and Maria Fernandez. "Bioactive components in Moringa Oleifera leaves protect against chronic disease." Antioxidants 6, no. 4 (2017): 91.
[18] Oyedepo, T. A., S. O. Babarinde, and T. A. Ajayeoba. "Evaluation of anti-hyperlipidemic effect of aqueous leaves extract of Moringa oleifera in alloxan induced diabetic rats." International Journal of Biochemistry Research & Review 3, no. 3 (2013): 162.
[19] Malum, Yarima Muhammad, and LabaranSalihu. "Determination of Heavy Metals in Hibiscus cannabinus and Moringaoleifera Cultivated at Zango Abattoir, Tudun Wada, Kaduna Metropolis." International Journal of Sustainable Development Research 3, no. 4 (2017): 32.
[20] Munro, A., and O. Bassir. "Oxalate in Nigerian vegetables." West African Journal of Biology and Applied Chemistry 12, no. 1 (1969): 4-18.
[21] Griffiths, D. Wynne, and T. Arthur Thomas. "Phytate and total phosphorus content of field beans (Viciafaba L.)." Journal of the Science of Food and Agriculture 32, no. 2 (1981): 187-192.
[22] Association of Official Analytical Chemists, AOAC, (1999). Methods of analysis of Association of Official Analytical Chemists. 16th ed. Washington DC. 1: 600-792.
[23] FAO/WHO. Food additives and contaminants, Joint Codex Alimentarius Commission, FAO/WHO. Food standards Programme, ALINORM 01/12A. 2001.
[24] Verkaik-Kloosterman, Janneke, MaryT McCann, Jeljer Hoekstra, and Hans Verhagen. "Vitamins and minerals: issues associated with too low and too high population intakes." Food & nutrition research 56, no. 1 (2012): 5728.
[25] Opaluwa OD, Aremu MO, Ogbo LO, Abiola, KA, Nweze, NO. Heavy metal concentrations in soils, plant leaves and crops grown around dump sites in Lafia Metropolis, Nasarawa State, Nigeria. Advances in Applied Science Research, 2012; 3 (2): 780-784.
[26] Maynard, L. A. (1997). Animal Nutrition. McGraw Hill Book Company Ltd. New York. Pp 47-79.
[27] Kidmose, Ulla, R-Y. Yang, S. H. Thilsted, Lars Porskjær Christensen, and Kirsten Brandt. "Content of carotenoids in commonly consumed Asian vegetables and stability and extractability during frying." Journal of Food Composition and Analysis 19, no. 6-7 (2006): 562-571.
[28] Ekop, A. S., and N. O. Eddy. "Comparative studies of the level of toxicants in the seed of Indian almond (Terminaliacatappa) and African walnut (Coulaedulis). Chem." Class J 2 (2005): 74-76.
[29] Makkar, H. P. S., and K. Becker. "Nutrients and antiquality factors in different morphological parts of the Moringaoleifera tree." The Journal of Agricultural Science 128, no. 3 (1997): 311-322.
[30] Harborne, J. B. (2006). “Pharmacological application of plant phytochemicals”. Phytochemical methods: A guide to morden techniques of plant analysis. Chapman and Hall, London. (3rded). Pp 211-217.
[31] OKWU, DONATUS E. "The potentials of Ocimumgratissimum, Penrgulariaextensa and Tetrapleuratetraptera as spice and flavouring agents." Nigeria Agricultural Journal 34, no. 1 (2003): 143-148.
Cite This Article
  • APA Style

    Niaz Mahmud, Monirul Islam, Md. Shovon Al-Fuad, Samiron Sana, Md. Jannatul Ferdaus, et al. (2019). Estimation of Heavy Metals, Essential Trace Elements and Anti-Nutritional Factors in Leaves and Stems from Moringa oleifera. International Journal of Food Science and Biotechnology, 4(2), 51-55. https://doi.org/10.11648/j.ijfsb.20190402.14

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    ACS Style

    Niaz Mahmud; Monirul Islam; Md. Shovon Al-Fuad; Samiron Sana; Md. Jannatul Ferdaus, et al. Estimation of Heavy Metals, Essential Trace Elements and Anti-Nutritional Factors in Leaves and Stems from Moringa oleifera. Int. J. Food Sci. Biotechnol. 2019, 4(2), 51-55. doi: 10.11648/j.ijfsb.20190402.14

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    AMA Style

    Niaz Mahmud, Monirul Islam, Md. Shovon Al-Fuad, Samiron Sana, Md. Jannatul Ferdaus, et al. Estimation of Heavy Metals, Essential Trace Elements and Anti-Nutritional Factors in Leaves and Stems from Moringa oleifera. Int J Food Sci Biotechnol. 2019;4(2):51-55. doi: 10.11648/j.ijfsb.20190402.14

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  • @article{10.11648/j.ijfsb.20190402.14,
      author = {Niaz Mahmud and Monirul Islam and Md. Shovon Al-Fuad and Samiron Sana and Md. Jannatul Ferdaus and Suzon Ahmed and Shahriar Islam Satya and Md. Abdullah Al Mamun and Nazmus Sakib and Md. Shofiqul Islam and Shuvonkar Kangsha Bonik},
      title = {Estimation of Heavy Metals, Essential Trace Elements and Anti-Nutritional Factors in Leaves and Stems from Moringa oleifera},
      journal = {International Journal of Food Science and Biotechnology},
      volume = {4},
      number = {2},
      pages = {51-55},
      doi = {10.11648/j.ijfsb.20190402.14},
      url = {https://doi.org/10.11648/j.ijfsb.20190402.14},
      eprint = {https://download.sciencepg.com/pdf/10.11648.j.ijfsb.20190402.14},
      abstract = {This study was conducted to estimate the concentrations of 8 trace elements including (Mn, Fe, Cu, Zn, Ni, Ar, Cr, and Pb) and 4 anti-nutritional factors including (saponins, oxalates, phytate, and cyanogenic glycosides) in leaves and stems from Moringa oleifera. The concentrations in samples analyzed were found to be in the range of 0.88-1.88 mg/kg for Mn, 11.95-25.25 mg/kg for Fe, 0.35-1.22 mg/kg for Cu, 6.92-17.96 mg/kg for Zn, 0.03-0.07 mg/kg for Ni, 0.955-1.45 mg/kg for Cr and 0.564-0.85 mg/kg for Pb. However, Arsenic was not detected in all the samples analyzed. As for the anti-nutritional factors, the concentrations in samples analyzed were found to be in the range of 111.35-123.42 mg/kg for saponins, 69.5-509.4 mg/kg for oxalates, and 0.38-0.156 mg/kg for phytate and 316.95-325.27 mg/kg for cyanogenic glycosides. The values of all these elements were found significantly below the recommended maximum tolerable guidelines level proposed by WHO/FAO except for lead (Pb), Pb was found slightly higher than the recommended limit as described. Our findings of this study reveal that most of the trace elements found in M. oleifera are below the recommended maximum tolerable limits; therefore it is safe for both human and animal consumption as well.},
     year = {2019}
    }
    

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  • TY  - JOUR
    T1  - Estimation of Heavy Metals, Essential Trace Elements and Anti-Nutritional Factors in Leaves and Stems from Moringa oleifera
    AU  - Niaz Mahmud
    AU  - Monirul Islam
    AU  - Md. Shovon Al-Fuad
    AU  - Samiron Sana
    AU  - Md. Jannatul Ferdaus
    AU  - Suzon Ahmed
    AU  - Shahriar Islam Satya
    AU  - Md. Abdullah Al Mamun
    AU  - Nazmus Sakib
    AU  - Md. Shofiqul Islam
    AU  - Shuvonkar Kangsha Bonik
    Y1  - 2019/07/24
    PY  - 2019
    N1  - https://doi.org/10.11648/j.ijfsb.20190402.14
    DO  - 10.11648/j.ijfsb.20190402.14
    T2  - International Journal of Food Science and Biotechnology
    JF  - International Journal of Food Science and Biotechnology
    JO  - International Journal of Food Science and Biotechnology
    SP  - 51
    EP  - 55
    PB  - Science Publishing Group
    SN  - 2578-9643
    UR  - https://doi.org/10.11648/j.ijfsb.20190402.14
    AB  - This study was conducted to estimate the concentrations of 8 trace elements including (Mn, Fe, Cu, Zn, Ni, Ar, Cr, and Pb) and 4 anti-nutritional factors including (saponins, oxalates, phytate, and cyanogenic glycosides) in leaves and stems from Moringa oleifera. The concentrations in samples analyzed were found to be in the range of 0.88-1.88 mg/kg for Mn, 11.95-25.25 mg/kg for Fe, 0.35-1.22 mg/kg for Cu, 6.92-17.96 mg/kg for Zn, 0.03-0.07 mg/kg for Ni, 0.955-1.45 mg/kg for Cr and 0.564-0.85 mg/kg for Pb. However, Arsenic was not detected in all the samples analyzed. As for the anti-nutritional factors, the concentrations in samples analyzed were found to be in the range of 111.35-123.42 mg/kg for saponins, 69.5-509.4 mg/kg for oxalates, and 0.38-0.156 mg/kg for phytate and 316.95-325.27 mg/kg for cyanogenic glycosides. The values of all these elements were found significantly below the recommended maximum tolerable guidelines level proposed by WHO/FAO except for lead (Pb), Pb was found slightly higher than the recommended limit as described. Our findings of this study reveal that most of the trace elements found in M. oleifera are below the recommended maximum tolerable limits; therefore it is safe for both human and animal consumption as well.
    VL  - 4
    IS  - 2
    ER  - 

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Author Information
  • Department of Nutrition and Food Technology, Jashore University of Science and Technology, Jashore, Bangladesh

  • State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, China; Rural Development Academy (RDA), Bogra, Bangladesh

  • Department of Nutrition and Food Technology, Jashore University of Science and Technology, Jashore, Bangladesh

  • Pharmacy Discipline, Khulna University, Khulna, Bangladesh

  • Department of Nutrition and Food Technology, Jashore University of Science and Technology, Jashore, Bangladesh

  • Department of Nutrition and Food Technology, Jashore University of Science and Technology, Jashore, Bangladesh

  • Department of Nutrition and Food Technology, Jashore University of Science and Technology, Jashore, Bangladesh

  • Department of Nutrition and Food Technology, Jashore University of Science and Technology, Jashore, Bangladesh

  • Department of Genetic Engineering and Biotechnology, Jashore University of Science and Technology, Jashore, Bangladesh

  • Department of Genetic Engineering and Biotechnology, Jashore University of Science and Technology, Jashore, Bangladesh

  • Department of Nutrition and Food Technology, Jashore University of Science and Technology, Jashore, Bangladesh

  • Section