American Journal of Biomedical and Life Sciences

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Conventional and Molecular Diagnostic Tests for the Detection of Bacterial Pathogen in Burn Wound and Antimicrobial Properties of Some Medicinal Plants

Received: Jan. 05, 2020    Accepted: Jan. 15, 2020    Published: Mar. 24, 2020
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Abstract

This research aims to compare bacterial pathogens in different categories of burn wounds and evaluate the specificity and sensitivity of conventional and molecular diagnostics techniques in the detection of bacterial pathogens in burn wounds. This research project also tends to evaluate the potential antimicrobial activity of natural product by using Vachellia nilotica and Prosopis africana plant extracts. Burn wounds synovial fluid was collected from 50 patients each from three categories of burn wound over a period of 14 months. Samples were subjected to conventional and molecular diagnosis for the screening of the bacterial pathogens. Antibacterial properties of the plants extracts were tested using disc diffusion technique. Of the 50 samples, P. aeruginosa were isolated from 7 (14%), 12 (24%) and 17 (34%) samples of first, second and third degree of burn wounds respectively and were considered both positive for P. aeruginosa infection. However, Staphylococcus aureus was isolated only in the third degree from 4 out of 50 samples and was considered to be negative from first and second degree. Coliform (except E. coli) were absent from first degree but present in both second and third degrees (4 and 6) respectively. Gram stain can be considered as a rapid test but solely depend on the microbiological culture test, likewise majority of the biochemical test such as oxidase and API 20E tests. It was discovered that there is highest sensitivity of PCR over culture and or biochemical tests in the detection of P. aeruginosa from burn wound patients while some found no difference or even lower sensitivity in PCR assay. The result shows relatively antibacterial properties of both plant extracts against P. aeruginosa. It is concluded that P. aeruginosa is the most prevailing bacterial pathogen in burn wound and these plants extracts are active against the pathogen. Finally, a research to isolate and test individual chemical compounds responsible for the antibacterial properties from these plants is highly recommended.

DOI 10.11648/j.ajbls.20200802.11
Published in American Journal of Biomedical and Life Sciences ( Volume 8, Issue 2, April 2020 )
Page(s) 25-32
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Conventional, Molecular, Antibacteria and Wound

References
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[2] P. Deschaght, T. De Baere, L. Van Simaey, S. Van daele, F. De Baets, D. De Vos, J. P. Pirnay and M. Vaneechoutte (2009). Comparison of the Sensitivity of Culture, PCRand Quantitative Real-time PCR for the Detection of Pseudomonasaeruginosa in Sputum of Cystic Fibrosis Patients. BMC Microbiol, 9: pp. 244.
[3] M. P. Weinstein, M. L. Towns, S. M. Quartey, S. Mirrett, L. G. Reimer, G. Parmigiani, and L. B. Reller (1997). The Clinical Significance of Positive Blood Cultures in the 1990s: A Prospective Comprehensive Evaluation of the Microbiology, Epidemiology, and Outcome of Bacteremia and Fungemia in Adults. Clinical Infectious Diseases, 24 (4), pp. 584-602.
[4] Van Belkum A, Renders NHM, Smith S, Overbeek SE, Verbrugh HA (2000). Comparison Of Conventional and Molecular Methods for the Detection of Bacterial Pathogens in Sputum Samples From Cystic Fibrosis. FEMSImmunol Med Microbiol, pp 27: 51-57.
[5] C. K. Stover, X. Q. Pham, A. L. Erwin, S. D. Mizoguchi, P. Warrener, M. J. Hickey, F. S. L. Brinkman, W. O. Hufnagle, D. J. Kowalik, M. Lagrou, and R. P. Garber (2000). Complete Genome Sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen. Nature, 406 (6799), pp. 959-964.
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[9] N. H. Wagini, S. A. Mohamed, A. S Soliman, Y. A. Hanafy, E. Badawy (2014). In Vitro and in Vivo Anti Dermatophytes Activity of Lawsonia inermis L. (Henna) Leaves against Ringworm and Its Etiological Agents. American Journal of Clinical and Experimental Medicine. Vol. 2, No. 3. ajcem. 203.13.
[10] D. Herndon, (2012). "Chapter 4: Prevention of Burn Injuries". Total Burn Care (4th edition.). Edinburgh: Saunders. P/46. ISBN 978-1-4377-2786-9.
[11] D. C. Nwonkonkwo (2009). Phytochemical Constituents and Anti-Microbial Activity of the Stem Bark Extract of Ficusaspergulia (Sand Paper Tree). Journal of the Chemical Society of Nigeria. 34 (2): 119-122.
[12] A. E. Sofowora (2010). Medicainal Plants and Traditional Medicine in Africa. 2nd Edition. Spectrum Book Limited Ibadan Nigeria. Pp 142-144.
[13] R. A. Oboh (2004). Anti-microbial Activities of Euphobia and Erigenron. Nigeria Annan’s of Natural Sciences. 5 (1): 72-77.
[14] M. R. Gonzalez, B. Fleuchot, L. Lauciell, P. Jafari, L. A. Applegate, W. Raffoul, Y. A. Que, and K Perron K. (2016). Effect of human Burn Wound Exudate on Pseudomonas aeruginosa virulence. mSphere 1 (2). 00111-15.
[15] G. I. Yar'gamji (2011). Preliminary Phytochemical Screening of the Aqueous Extract of Parking Biglobosa (Dorowa). ChemclassJournal. 4 (1): 1-9.
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    Nasir Hassan Wagini, Amina Lema Rafukka, Aliyu Musa Yusuf, Sani Muhd Gidado, Samaila Abubakar, et al. (2020). Conventional and Molecular Diagnostic Tests for the Detection of Bacterial Pathogen in Burn Wound and Antimicrobial Properties of Some Medicinal Plants. American Journal of Biomedical and Life Sciences, 8(2), 25-32. https://doi.org/10.11648/j.ajbls.20200802.11

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    ACS Style

    Nasir Hassan Wagini; Amina Lema Rafukka; Aliyu Musa Yusuf; Sani Muhd Gidado; Samaila Abubakar, et al. Conventional and Molecular Diagnostic Tests for the Detection of Bacterial Pathogen in Burn Wound and Antimicrobial Properties of Some Medicinal Plants. Am. J. Biomed. Life Sci. 2020, 8(2), 25-32. doi: 10.11648/j.ajbls.20200802.11

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    AMA Style

    Nasir Hassan Wagini, Amina Lema Rafukka, Aliyu Musa Yusuf, Sani Muhd Gidado, Samaila Abubakar, et al. Conventional and Molecular Diagnostic Tests for the Detection of Bacterial Pathogen in Burn Wound and Antimicrobial Properties of Some Medicinal Plants. Am J Biomed Life Sci. 2020;8(2):25-32. doi: 10.11648/j.ajbls.20200802.11

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  • @article{10.11648/j.ajbls.20200802.11,
      author = {Nasir Hassan Wagini and Amina Lema Rafukka and Aliyu Musa Yusuf and Sani Muhd Gidado and Samaila Abubakar and Mudassiru Badamasi and Abubakar Mannir Darma and Taofiq Ademola Babatunde and Abubakar Bello and Murtala Yusuf and Jibrin Naka Keta and Lawan Buba Amshi and Hussaina Usman Babba},
      title = {Conventional and Molecular Diagnostic Tests for the Detection of Bacterial Pathogen in Burn Wound and Antimicrobial Properties of Some Medicinal Plants},
      journal = {American Journal of Biomedical and Life Sciences},
      volume = {8},
      number = {2},
      pages = {25-32},
      doi = {10.11648/j.ajbls.20200802.11},
      url = {https://doi.org/10.11648/j.ajbls.20200802.11},
      eprint = {https://download.sciencepg.com/pdf/10.11648.j.ajbls.20200802.11},
      abstract = {This research aims to compare bacterial pathogens in different categories of burn wounds and evaluate the specificity and sensitivity of conventional and molecular diagnostics techniques in the detection of bacterial pathogens in burn wounds. This research project also tends to evaluate the potential antimicrobial activity of natural product by using Vachellia nilotica and Prosopis africana plant extracts. Burn wounds synovial fluid was collected from 50 patients each from three categories of burn wound over a period of 14 months. Samples were subjected to conventional and molecular diagnosis for the screening of the bacterial pathogens. Antibacterial properties of the plants extracts were tested using disc diffusion technique. Of the 50 samples, P. aeruginosa were isolated from 7 (14%), 12 (24%) and 17 (34%) samples of first, second and third degree of burn wounds respectively and were considered both positive for P. aeruginosa infection. However, Staphylococcus aureus was isolated only in the third degree from 4 out of 50 samples and was considered to be negative from first and second degree. Coliform (except E. coli) were absent from first degree but present in both second and third degrees (4 and 6) respectively. Gram stain can be considered as a rapid test but solely depend on the microbiological culture test, likewise majority of the biochemical test such as oxidase and API 20E tests. It was discovered that there is highest sensitivity of PCR over culture and or biochemical tests in the detection of P. aeruginosa from burn wound patients while some found no difference or even lower sensitivity in PCR assay. The result shows relatively antibacterial properties of both plant extracts against P. aeruginosa. It is concluded that P. aeruginosa is the most prevailing bacterial pathogen in burn wound and these plants extracts are active against the pathogen. Finally, a research to isolate and test individual chemical compounds responsible for the antibacterial properties from these plants is highly recommended.},
     year = {2020}
    }
    

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    T1  - Conventional and Molecular Diagnostic Tests for the Detection of Bacterial Pathogen in Burn Wound and Antimicrobial Properties of Some Medicinal Plants
    AU  - Nasir Hassan Wagini
    AU  - Amina Lema Rafukka
    AU  - Aliyu Musa Yusuf
    AU  - Sani Muhd Gidado
    AU  - Samaila Abubakar
    AU  - Mudassiru Badamasi
    AU  - Abubakar Mannir Darma
    AU  - Taofiq Ademola Babatunde
    AU  - Abubakar Bello
    AU  - Murtala Yusuf
    AU  - Jibrin Naka Keta
    AU  - Lawan Buba Amshi
    AU  - Hussaina Usman Babba
    Y1  - 2020/03/24
    PY  - 2020
    N1  - https://doi.org/10.11648/j.ajbls.20200802.11
    DO  - 10.11648/j.ajbls.20200802.11
    T2  - American Journal of Biomedical and Life Sciences
    JF  - American Journal of Biomedical and Life Sciences
    JO  - American Journal of Biomedical and Life Sciences
    SP  - 25
    EP  - 32
    PB  - Science Publishing Group
    SN  - 2330-880X
    UR  - https://doi.org/10.11648/j.ajbls.20200802.11
    AB  - This research aims to compare bacterial pathogens in different categories of burn wounds and evaluate the specificity and sensitivity of conventional and molecular diagnostics techniques in the detection of bacterial pathogens in burn wounds. This research project also tends to evaluate the potential antimicrobial activity of natural product by using Vachellia nilotica and Prosopis africana plant extracts. Burn wounds synovial fluid was collected from 50 patients each from three categories of burn wound over a period of 14 months. Samples were subjected to conventional and molecular diagnosis for the screening of the bacterial pathogens. Antibacterial properties of the plants extracts were tested using disc diffusion technique. Of the 50 samples, P. aeruginosa were isolated from 7 (14%), 12 (24%) and 17 (34%) samples of first, second and third degree of burn wounds respectively and were considered both positive for P. aeruginosa infection. However, Staphylococcus aureus was isolated only in the third degree from 4 out of 50 samples and was considered to be negative from first and second degree. Coliform (except E. coli) were absent from first degree but present in both second and third degrees (4 and 6) respectively. Gram stain can be considered as a rapid test but solely depend on the microbiological culture test, likewise majority of the biochemical test such as oxidase and API 20E tests. It was discovered that there is highest sensitivity of PCR over culture and or biochemical tests in the detection of P. aeruginosa from burn wound patients while some found no difference or even lower sensitivity in PCR assay. The result shows relatively antibacterial properties of both plant extracts against P. aeruginosa. It is concluded that P. aeruginosa is the most prevailing bacterial pathogen in burn wound and these plants extracts are active against the pathogen. Finally, a research to isolate and test individual chemical compounds responsible for the antibacterial properties from these plants is highly recommended.
    VL  - 8
    IS  - 2
    ER  - 

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Author Information
  • Department of Biology, Faculty of Natural and Applied Sciences, Umaru Musa Yar’adua University, Katsina, Nigeria

  • Department of Biology, Faculty of Natural and Applied Sciences, Umaru Musa Yar’adua University, Katsina, Nigeria

  • Department of Biology, Faculty of Natural and Applied Sciences, Umaru Musa Yar’adua University, Katsina, Nigeria

  • Department of Biology, Faculty of Natural and Applied Sciences, Umaru Musa Yar’adua University, Katsina, Nigeria

  • Department of Biology, Faculty of Natural and Applied Sciences, Umaru Musa Yar’adua University, Katsina, Nigeria

  • Department of Biology, Faculty of Natural and Applied Sciences, Umaru Musa Yar’adua University, Katsina, Nigeria

  • Department of Biology, Faculty of Natural and Applied Sciences, Umaru Musa Yar’adua University, Katsina, Nigeria

  • Department of Biology, Faculty of Natural and Applied Sciences, Umaru Musa Yar’adua University, Katsina, Nigeria

  • Department of Biology, Faculty of Natural and Applied Sciences, Umaru Musa Yar’adua University, Katsina, Nigeria

  • Department of Biology, College of Natural and Applied Sciences, Alqalam University, Katsina, Nigeria

  • Department of Biological Sciences, Faculty of Science, Kebbi State University of Science and Technology, Aliero, Kebbi, Nigeria

  • Department of Biological Sciences, Faculty of Science, Yobe State University, Damaturu, Nigeria

  • Department of Animal Science, Faculty of Agriculture, Federal University, Dutsin-ma, Katsina, Nigeria

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