The Effect of Sirtuin 1 Inhibitor Ex-527 and Activator Resveratrol on the Oocytes’ Cells Viability in Mice Model of Experimental Systemic Autoimmune Damage

Mariia Stupchuk; Alina Lytvynenko; Tetyana Voznesenska

doi:doi:10.11648/j.aap.20210602.15

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The Effect of Sirtuin 1 Inhibitor Ex-527 and Activator Resveratrol on the Oocytes’ Cells Viability in Mice Model of Experimental Systemic Autoimmune Damage

Mariia Stupchuk, Alina Lytvynenko, Tetyana Voznesenska

Published in Advances in Applied Physiology (Volume 6, Issue 2)

Received: 26 October 2021 Accepted: 22 November 2021 Published: 29 November 2021

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Abstract

Introduction: Female infertility is a very common pathology for women of reproductive age with autoimmune disorders, which are known to be followed by the abnormal increment of reactive oxygen species levels, moreover, further contributing to follicular atresia and aging of oocytes in the ovaries what directly leads to infertility. Thus, in search for strategies of preventing oxidative threat to ovaries, the current study was aimed to assess the influence of sirtuin 1, a key cellular metabolism regulator and oxidative stress, activator/blocker on the viability of the follicular environment of oocytes (FEO) cells under conditions of experimental systemic autoimmune damage (ESAD). Methods: The study was performed using the model of ESAD on female mice. The FEO cells were cultivated in different conditions: resveratrol 20 µM, Ex-527 20 µM. After 24h, the cells were examined for viability; the ways of cell death via apoptosis, necrosis were estimated using the method of in vivo dual-color with fluorescent nucleic acid dyes Hoechst 33342 and propidium iodide, and the levels of autophagy were estimated using the autophagic vacuoles labeling with monodansylcadaverine assay. Results: The obtained data indicate that a specific inhibitor of sirtuin 1 Ex-527 (20 µM) in vitro inhibits the viability of cells of the FEO cells and increases the percent of cell death via autophagy, apoptosis, and necrosis. On the contrary, the activator of sirtuin 1 - resveratrol, led to an improvement of the viability status of FEO cells, while reducing the negative impact of the inflammatory process. Unidirectional action of Ex-527 and resveratrol compounds at the cellular level has been established. Conclusion: Thus, the results of the present study suggest the involvement of sirtuin 1 in the regulation of the damaging effect of reactive oxygen species on female ovarian cells under the conditions of experimental systemic autoimmune damage.

Published in	Advances in Applied Physiology (Volume 6, Issue 2)
DOI	10.11648/j.aap.20210602.15
Page(s)	47-52
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2021. Published by Science Publishing Group

Keywords

Ovarian Cells, Autophagy, Apoptosis, Sirtuins, Resveratrol, Ex-527, Systemic Autoimmune Damage

References

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Mariia Stupchuk, Alina Lytvynenko, Tetyana Voznesenska. (2021). The Effect of Sirtuin 1 Inhibitor Ex-527 and Activator Resveratrol on the Oocytes’ Cells Viability in Mice Model of Experimental Systemic Autoimmune Damage. Advances in Applied Physiology, 6(2), 47-52. https://doi.org/10.11648/j.aap.20210602.15

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ACS Style

Mariia Stupchuk; Alina Lytvynenko; Tetyana Voznesenska. The Effect of Sirtuin 1 Inhibitor Ex-527 and Activator Resveratrol on the Oocytes’ Cells Viability in Mice Model of Experimental Systemic Autoimmune Damage. Adv. Appl. Physiol. 2021, 6(2), 47-52. doi: 10.11648/j.aap.20210602.15

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AMA Style

Mariia Stupchuk, Alina Lytvynenko, Tetyana Voznesenska. The Effect of Sirtuin 1 Inhibitor Ex-527 and Activator Resveratrol on the Oocytes’ Cells Viability in Mice Model of Experimental Systemic Autoimmune Damage. Adv Appl Physiol. 2021;6(2):47-52. doi: 10.11648/j.aap.20210602.15

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@article{10.11648/j.aap.20210602.15,
  author = {Mariia Stupchuk and Alina Lytvynenko and Tetyana Voznesenska},
  title = {The Effect of Sirtuin 1 Inhibitor Ex-527 and Activator Resveratrol on the Oocytes’ Cells Viability in Mice Model of Experimental Systemic Autoimmune Damage},
  journal = {Advances in Applied Physiology},
  volume = {6},
  number = {2},
  pages = {47-52},
  doi = {10.11648/j.aap.20210602.15},
  url = {https://doi.org/10.11648/j.aap.20210602.15},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.aap.20210602.15},
  abstract = {Introduction: Female infertility is a very common pathology for women of reproductive age with autoimmune disorders, which are known to be followed by the abnormal increment of reactive oxygen species levels, moreover, further contributing to follicular atresia and aging of oocytes in the ovaries what directly leads to infertility. Thus, in search for strategies of preventing oxidative threat to ovaries, the current study was aimed to assess the influence of sirtuin 1, a key cellular metabolism regulator and oxidative stress, activator/blocker on the viability of the follicular environment of oocytes (FEO) cells under conditions of experimental systemic autoimmune damage (ESAD). Methods: The study was performed using the model of ESAD on female mice. The FEO cells were cultivated in different conditions: resveratrol 20 µM, Ex-527 20 µM. After 24h, the cells were examined for viability; the ways of cell death via apoptosis, necrosis were estimated using the method of in vivo dual-color with fluorescent nucleic acid dyes Hoechst 33342 and propidium iodide, and the levels of autophagy were estimated using the autophagic vacuoles labeling with monodansylcadaverine assay. Results: The obtained data indicate that a specific inhibitor of sirtuin 1 Ex-527 (20 µM) in vitro inhibits the viability of cells of the FEO cells and increases the percent of cell death via autophagy, apoptosis, and necrosis. On the contrary, the activator of sirtuin 1 - resveratrol, led to an improvement of the viability status of FEO cells, while reducing the negative impact of the inflammatory process. Unidirectional action of Ex-527 and resveratrol compounds at the cellular level has been established. Conclusion: Thus, the results of the present study suggest the involvement of sirtuin 1 in the regulation of the damaging effect of reactive oxygen species on female ovarian cells under the conditions of experimental systemic autoimmune damage.},
 year = {2021}
}

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TY  - JOUR
T1  - The Effect of Sirtuin 1 Inhibitor Ex-527 and Activator Resveratrol on the Oocytes’ Cells Viability in Mice Model of Experimental Systemic Autoimmune Damage
AU  - Mariia Stupchuk
AU  - Alina Lytvynenko
AU  - Tetyana Voznesenska
Y1  - 2021/11/29
PY  - 2021
N1  - https://doi.org/10.11648/j.aap.20210602.15
DO  - 10.11648/j.aap.20210602.15
T2  - Advances in Applied Physiology
JF  - Advances in Applied Physiology
JO  - Advances in Applied Physiology
SP  - 47
EP  - 52
PB  - Science Publishing Group
SN  - 2471-9714
UR  - https://doi.org/10.11648/j.aap.20210602.15
AB  - Introduction: Female infertility is a very common pathology for women of reproductive age with autoimmune disorders, which are known to be followed by the abnormal increment of reactive oxygen species levels, moreover, further contributing to follicular atresia and aging of oocytes in the ovaries what directly leads to infertility. Thus, in search for strategies of preventing oxidative threat to ovaries, the current study was aimed to assess the influence of sirtuin 1, a key cellular metabolism regulator and oxidative stress, activator/blocker on the viability of the follicular environment of oocytes (FEO) cells under conditions of experimental systemic autoimmune damage (ESAD). Methods: The study was performed using the model of ESAD on female mice. The FEO cells were cultivated in different conditions: resveratrol 20 µM, Ex-527 20 µM. After 24h, the cells were examined for viability; the ways of cell death via apoptosis, necrosis were estimated using the method of in vivo dual-color with fluorescent nucleic acid dyes Hoechst 33342 and propidium iodide, and the levels of autophagy were estimated using the autophagic vacuoles labeling with monodansylcadaverine assay. Results: The obtained data indicate that a specific inhibitor of sirtuin 1 Ex-527 (20 µM) in vitro inhibits the viability of cells of the FEO cells and increases the percent of cell death via autophagy, apoptosis, and necrosis. On the contrary, the activator of sirtuin 1 - resveratrol, led to an improvement of the viability status of FEO cells, while reducing the negative impact of the inflammatory process. Unidirectional action of Ex-527 and resveratrol compounds at the cellular level has been established. Conclusion: Thus, the results of the present study suggest the involvement of sirtuin 1 in the regulation of the damaging effect of reactive oxygen species on female ovarian cells under the conditions of experimental systemic autoimmune damage.
VL  - 6
IS  - 2
ER  -

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Author Information

Mariia Stupchuk

Department of Immunophysiology, Bogomoletz Institute of Physiology of National Academy of Sciences of Ukraine, Kyiv, Ukraine
Alina Lytvynenko

Department of Immunophysiology, Bogomoletz Institute of Physiology of National Academy of Sciences of Ukraine, Kyiv, Ukraine
Tetyana Voznesenska

Department of Immunophysiology, Bogomoletz Institute of Physiology of National Academy of Sciences of Ukraine, Kyiv, Ukraine

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Mariia Stupchuk, Alina Lytvynenko, Tetyana Voznesenska. (2021). The Effect of Sirtuin 1 Inhibitor Ex-527 and Activator Resveratrol on the Oocytes’ Cells Viability in Mice Model of Experimental Systemic Autoimmune Damage. Advances in Applied Physiology, 6(2), 47-52. https://doi.org/10.11648/j.aap.20210602.15

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Mariia Stupchuk; Alina Lytvynenko; Tetyana Voznesenska. The Effect of Sirtuin 1 Inhibitor Ex-527 and Activator Resveratrol on the Oocytes’ Cells Viability in Mice Model of Experimental Systemic Autoimmune Damage. Adv. Appl. Physiol. 2021, 6(2), 47-52. doi: 10.11648/j.aap.20210602.15

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AMA Style

Mariia Stupchuk, Alina Lytvynenko, Tetyana Voznesenska. The Effect of Sirtuin 1 Inhibitor Ex-527 and Activator Resveratrol on the Oocytes’ Cells Viability in Mice Model of Experimental Systemic Autoimmune Damage. Adv Appl Physiol. 2021;6(2):47-52. doi: 10.11648/j.aap.20210602.15

Copy | Download

@article{10.11648/j.aap.20210602.15,
  author = {Mariia Stupchuk and Alina Lytvynenko and Tetyana Voznesenska},
  title = {The Effect of Sirtuin 1 Inhibitor Ex-527 and Activator Resveratrol on the Oocytes’ Cells Viability in Mice Model of Experimental Systemic Autoimmune Damage},
  journal = {Advances in Applied Physiology},
  volume = {6},
  number = {2},
  pages = {47-52},
  doi = {10.11648/j.aap.20210602.15},
  url = {https://doi.org/10.11648/j.aap.20210602.15},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.aap.20210602.15},
  abstract = {Introduction: Female infertility is a very common pathology for women of reproductive age with autoimmune disorders, which are known to be followed by the abnormal increment of reactive oxygen species levels, moreover, further contributing to follicular atresia and aging of oocytes in the ovaries what directly leads to infertility. Thus, in search for strategies of preventing oxidative threat to ovaries, the current study was aimed to assess the influence of sirtuin 1, a key cellular metabolism regulator and oxidative stress, activator/blocker on the viability of the follicular environment of oocytes (FEO) cells under conditions of experimental systemic autoimmune damage (ESAD). Methods: The study was performed using the model of ESAD on female mice. The FEO cells were cultivated in different conditions: resveratrol 20 µM, Ex-527 20 µM. After 24h, the cells were examined for viability; the ways of cell death via apoptosis, necrosis were estimated using the method of in vivo dual-color with fluorescent nucleic acid dyes Hoechst 33342 and propidium iodide, and the levels of autophagy were estimated using the autophagic vacuoles labeling with monodansylcadaverine assay. Results: The obtained data indicate that a specific inhibitor of sirtuin 1 Ex-527 (20 µM) in vitro inhibits the viability of cells of the FEO cells and increases the percent of cell death via autophagy, apoptosis, and necrosis. On the contrary, the activator of sirtuin 1 - resveratrol, led to an improvement of the viability status of FEO cells, while reducing the negative impact of the inflammatory process. Unidirectional action of Ex-527 and resveratrol compounds at the cellular level has been established. Conclusion: Thus, the results of the present study suggest the involvement of sirtuin 1 in the regulation of the damaging effect of reactive oxygen species on female ovarian cells under the conditions of experimental systemic autoimmune damage.},
 year = {2021}
}

Copy | Download

TY  - JOUR
T1  - The Effect of Sirtuin 1 Inhibitor Ex-527 and Activator Resveratrol on the Oocytes’ Cells Viability in Mice Model of Experimental Systemic Autoimmune Damage
AU  - Mariia Stupchuk
AU  - Alina Lytvynenko
AU  - Tetyana Voznesenska
Y1  - 2021/11/29
PY  - 2021
N1  - https://doi.org/10.11648/j.aap.20210602.15
DO  - 10.11648/j.aap.20210602.15
T2  - Advances in Applied Physiology
JF  - Advances in Applied Physiology
JO  - Advances in Applied Physiology
SP  - 47
EP  - 52
PB  - Science Publishing Group
SN  - 2471-9714
UR  - https://doi.org/10.11648/j.aap.20210602.15
AB  - Introduction: Female infertility is a very common pathology for women of reproductive age with autoimmune disorders, which are known to be followed by the abnormal increment of reactive oxygen species levels, moreover, further contributing to follicular atresia and aging of oocytes in the ovaries what directly leads to infertility. Thus, in search for strategies of preventing oxidative threat to ovaries, the current study was aimed to assess the influence of sirtuin 1, a key cellular metabolism regulator and oxidative stress, activator/blocker on the viability of the follicular environment of oocytes (FEO) cells under conditions of experimental systemic autoimmune damage (ESAD). Methods: The study was performed using the model of ESAD on female mice. The FEO cells were cultivated in different conditions: resveratrol 20 µM, Ex-527 20 µM. After 24h, the cells were examined for viability; the ways of cell death via apoptosis, necrosis were estimated using the method of in vivo dual-color with fluorescent nucleic acid dyes Hoechst 33342 and propidium iodide, and the levels of autophagy were estimated using the autophagic vacuoles labeling with monodansylcadaverine assay. Results: The obtained data indicate that a specific inhibitor of sirtuin 1 Ex-527 (20 µM) in vitro inhibits the viability of cells of the FEO cells and increases the percent of cell death via autophagy, apoptosis, and necrosis. On the contrary, the activator of sirtuin 1 - resveratrol, led to an improvement of the viability status of FEO cells, while reducing the negative impact of the inflammatory process. Unidirectional action of Ex-527 and resveratrol compounds at the cellular level has been established. Conclusion: Thus, the results of the present study suggest the involvement of sirtuin 1 in the regulation of the damaging effect of reactive oxygen species on female ovarian cells under the conditions of experimental systemic autoimmune damage.
VL  - 6
IS  - 2
ER  -

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