Fasting is abstaining from food and/or drinking water for a while, observed majorly for medical or spiritual purposes. The effect of varied fasting durations on intestinal glucose uptake was investigated in this study. Forty male Wistar rats, weighing between 100 and 120 g were used. They were randomly assigned into four groups (n=10 per group), Group 1: control (no fast), groups 2, 3, and 4, were fasted for 24, 48, and 72 hours respectively. Five rats each from the groups were used for the in vivo and ex vivo studies. The fasting blood glucose (FBG) level, intestinal glucose uptake (in vivo and ex vivo methods), and intestinal luminal electrolytes for sodium and potassium were determined. The FBG and the intestinal glucose uptake of the rats were determined by the glucose oxidase method. Data were represented as Mean±SEM and analysis was done by one-way ANOVA using GraphPad Prism version 7.0. Results were considered significant at p<0.05. The FBG (mg/dl) in the 24H (62.00±5.17), 48H (51.80±4.48) and 72H (75.00±8.32) groups decreased significantly compared with the control (103.80±4.35). Intestinal glucose concentration (mg/dl/g tissue) in vivo method decrease significantly in the 48H (11.21±0.38) and 72H (10.39±0.77) groups compared to the control (15.02±0.51) respectively in the jejunum and implied increased glucose absorption. Luminal glucose concentration (mg/dl/g tissue) of the fasted 48H group (27.92±0.66) increased significantly compared to control (13.86±0.75), 24H (17.84±0.65), and 72H (13.56±1.00). The ileum luminal glucose concentration (mg/dl/g tissue) decreased significantly in 48H (11.11±0.63) and 72H (11.02±0.56) groups compared to the control (13.86±0.59). In the assessment of varied fasting durations in vitro, intestinal glucose absorption increased while potassium concentration increased and sodium concentration decreased within the mucosa end. The results suggest that varied fasting periods increase intestinal glucose absorption. Conclusively, this study reported an overall increase in intestinal glucose absorption at both the jejunum and ileum ends after 48 and 72 h of continuous fasting.
| Published in | Advances in Applied Physiology (Volume 7, Issue 1) |
| DOI | 10.11648/j.aap.20220701.13 |
| Page(s) | 15-21 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2022. Published by Science Publishing Group |
Fasting, Intestinal Glucose Absorption, Electrolyte, Rats
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APA Style
Odukanmi Olugbenga Adeola. (2022). Varied Fasting Periods Moderate Intestinal Glucose Absorption in Male Wistar Rats. Advances in Applied Physiology, 7(1), 15-21. https://doi.org/10.11648/j.aap.20220701.13
ACS Style
Odukanmi Olugbenga Adeola. Varied Fasting Periods Moderate Intestinal Glucose Absorption in Male Wistar Rats. Adv. Appl. Physiol. 2022, 7(1), 15-21. doi: 10.11648/j.aap.20220701.13
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Download@article{10.11648/j.aap.20220701.13,
author = {Odukanmi Olugbenga Adeola},
title = {Varied Fasting Periods Moderate Intestinal Glucose Absorption in Male Wistar Rats},
journal = {Advances in Applied Physiology},
volume = {7},
number = {1},
pages = {15-21},
doi = {10.11648/j.aap.20220701.13},
url = {https://doi.org/10.11648/j.aap.20220701.13},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.aap.20220701.13},
abstract = {Fasting is abstaining from food and/or drinking water for a while, observed majorly for medical or spiritual purposes. The effect of varied fasting durations on intestinal glucose uptake was investigated in this study. Forty male Wistar rats, weighing between 100 and 120 g were used. They were randomly assigned into four groups (n=10 per group), Group 1: control (no fast), groups 2, 3, and 4, were fasted for 24, 48, and 72 hours respectively. Five rats each from the groups were used for the in vivo and ex vivo studies. The fasting blood glucose (FBG) level, intestinal glucose uptake (in vivo and ex vivo methods), and intestinal luminal electrolytes for sodium and potassium were determined. The FBG and the intestinal glucose uptake of the rats were determined by the glucose oxidase method. Data were represented as Mean±SEM and analysis was done by one-way ANOVA using GraphPad Prism version 7.0. Results were considered significant at p in vivo method decrease significantly in the 48H (11.21±0.38) and 72H (10.39±0.77) groups compared to the control (15.02±0.51) respectively in the jejunum and implied increased glucose absorption. Luminal glucose concentration (mg/dl/g tissue) of the fasted 48H group (27.92±0.66) increased significantly compared to control (13.86±0.75), 24H (17.84±0.65), and 72H (13.56±1.00). The ileum luminal glucose concentration (mg/dl/g tissue) decreased significantly in 48H (11.11±0.63) and 72H (11.02±0.56) groups compared to the control (13.86±0.59). In the assessment of varied fasting durations in vitro, intestinal glucose absorption increased while potassium concentration increased and sodium concentration decreased within the mucosa end. The results suggest that varied fasting periods increase intestinal glucose absorption. Conclusively, this study reported an overall increase in intestinal glucose absorption at both the jejunum and ileum ends after 48 and 72 h of continuous fasting.},
year = {2022}
}
TY - JOUR T1 - Varied Fasting Periods Moderate Intestinal Glucose Absorption in Male Wistar Rats AU - Odukanmi Olugbenga Adeola Y1 - 2022/04/26 PY - 2022 N1 - https://doi.org/10.11648/j.aap.20220701.13 DO - 10.11648/j.aap.20220701.13 T2 - Advances in Applied Physiology JF - Advances in Applied Physiology JO - Advances in Applied Physiology SP - 15 EP - 21 PB - Science Publishing Group SN - 2471-9714 UR - https://doi.org/10.11648/j.aap.20220701.13 AB - Fasting is abstaining from food and/or drinking water for a while, observed majorly for medical or spiritual purposes. The effect of varied fasting durations on intestinal glucose uptake was investigated in this study. Forty male Wistar rats, weighing between 100 and 120 g were used. They were randomly assigned into four groups (n=10 per group), Group 1: control (no fast), groups 2, 3, and 4, were fasted for 24, 48, and 72 hours respectively. Five rats each from the groups were used for the in vivo and ex vivo studies. The fasting blood glucose (FBG) level, intestinal glucose uptake (in vivo and ex vivo methods), and intestinal luminal electrolytes for sodium and potassium were determined. The FBG and the intestinal glucose uptake of the rats were determined by the glucose oxidase method. Data were represented as Mean±SEM and analysis was done by one-way ANOVA using GraphPad Prism version 7.0. Results were considered significant at p in vivo method decrease significantly in the 48H (11.21±0.38) and 72H (10.39±0.77) groups compared to the control (15.02±0.51) respectively in the jejunum and implied increased glucose absorption. Luminal glucose concentration (mg/dl/g tissue) of the fasted 48H group (27.92±0.66) increased significantly compared to control (13.86±0.75), 24H (17.84±0.65), and 72H (13.56±1.00). The ileum luminal glucose concentration (mg/dl/g tissue) decreased significantly in 48H (11.11±0.63) and 72H (11.02±0.56) groups compared to the control (13.86±0.59). In the assessment of varied fasting durations in vitro, intestinal glucose absorption increased while potassium concentration increased and sodium concentration decreased within the mucosa end. The results suggest that varied fasting periods increase intestinal glucose absorption. Conclusively, this study reported an overall increase in intestinal glucose absorption at both the jejunum and ileum ends after 48 and 72 h of continuous fasting. VL - 7 IS - 1 ER -
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