Evaluation of Hepatoprotective Activities Aqueous and Hydroethanolic Extracts from the Leaves of <i>Erythrococca anomala</i> in Rats

Miezan Bile Aka Patrice; Kouakou Sylvain Landry; Droucoula Guillaume Cyril; Kouakou-Syransy Giselle; Koffi Akissi Jeanne; Yapi Houphouet Felix

doi:doi:10.11648/j.ab.20170504.12

Evaluation of Hepatoprotective Activities Aqueous and Hydroethanolic Extracts from the Leaves of Erythrococca anomala in Rats

Miezan Bile Aka Patrice, Kouakou Sylvain Landry, Droucoula Guillaume Cyril, Kouakou-Syransy Giselle, Koffi Akissi Jeanne, Yapi Houphouet Felix

Published in Advances in Biochemistry (Volume 5, Issue 4)

Received: 18 May 2017 Accepted: 31 May 2017 Published: 13 July 2017

Views: Downloads:

Download PDF

Share This Article

Twitter
Linked In
Facebook

Abstract

Widely used in Ivory Coast and sub-Saharan Africa in traditional medicine as anti-inflammatory, antioxidant, laxative and purgative, Erythrococca anomala (Euphorbiaceae), is an annual plant. In this work, the aim was to evaluate the Hepatoprotective activity of the aqueous and hydroethanolic extracts of the leaves. Carbon Tetrachloride (CCl₄) was used to induce hepatotoxicity in rats. This hepatotoxic effect causes a significant increase in the levels of liver enzymes and serum proteins. Hepatoprotective activity was assessed by assaying liver enzymes such as serum transaminases (alanine aminotransferase and aspartate aminotransferase) and proteins by spectrophotometric and electrophoretic techniques. When administered orally as a preventive and curative dose of 100 and 200 mg / kg body weight, the extracts significantly reduce significantly as silymarin, the reference hepatoprotective substance at 100 mg / kg bw, hepatotoxicity induced by Carbon tetrachloride (CCL₄) at a dose of 5 mg / kg bw after a significant reduction in liver enzyme levels, globulins and a significant increase in albumin. The possible mechanism of this hepatoprotective activity of the extracts may be due to the action of the antioxidants such as the flavonoids, the phenols present in the extracts.

Published in	Advances in Biochemistry (Volume 5, Issue 4)
DOI	10.11648/j.ab.20170504.12
Page(s)	61-66
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2017. Published by Science Publishing Group

Keywords

Erythrococca anomala, Silymarin, Carbon Tetrachloride, Hepatotoxicity, Hepatoprotective

References

[1]	R. Islam, A. H. Alam, B. M. Rahman, K. A. Salam, A. Hossain, A. Baki, G. Sadik. Toxicological studies of two compounds isolated from Loranthus globosusRoxb. Pak. J. Biol. Sci., 10(2007) 2073-2077.
[2]	D. Kubmarawa, G. A. Ajoku, N. M. Enwerem, D. A. Okorie. Preliminaryphytochimical and antimicrobial screening of 50 medecinal plants from Nigeria, Afr. J. Biotechnol., 6 (2007) 1690-1696.
[3]	F. Calzada, L. Yepez-Mulia, A. Tapia-Contreras. Effect of Mexicanmedecinal plant used to treat tricomoniasis on Trichomonas vaginalistrophozoites. J. Ethnopharmacol. 113 (2007) 248-251.
[4]	Miezan BAP, Okpekon AT, Yapi HF, Bony FN, Gbassi G, Assi YJ. Chemical component and acute toxicity study of Erythrococca anomala (Euphorbiaceae). Asian Journal of Biomedical and Pharmaceutical Sciences. 2016; 6(57): 4-8. 5.
[5]	Oladele GM, Abatan MO, Olukynle JO, Okediran BS. Anti-inflammatory and analgesic effect of aqueous leafextracts of Gomphrenacelosioides and Momordicacharinda. J Nat. Sat. Sci. Engr. Tech. 2009; 8(2): 1-8.6.
[6]	Patrice M BA, Yapi HF, Opkekon AT, Bony FN, Kouakou-Siransy G, Kouakou YébouéKF. Evaluation of anti-inflammatory activities of Erythrococca anomalaaqueous and ethanolic extracts from leaves in rat. 14(2): 1-7, 2016; IJBCRR. 28385.
[7]	Miezan BAP, Okpekon AT, Koukou SL, Droucoula GC, Yapi HF. Evaluation of Antioxidant Activities of Erythrococca anomala Aqueous and Hydroethanolic Extracts. Journal ofBiotechnology and Biochemistry. Volume3, (2017), 113-118.
[8]	Adjanohoun EJ, Ake-Assi L. Contribution au recensement des plantes médicinales de Côte d’Ivoire. Centre National de Floristique de l’Université Nationale de Côte d’Ivoire, Tome 1, 23-30. Lavoisier, Paris. 1979; 895.
[9]	Guédé FG. Extraction of mansoninfromMansoniaaltissimaas cardiovascular agent (patent application) 1990. Ministère de la Recherche Scientifique, Côte d’Ivoire. 35 pp.
[10]	Raj, S., and Gothandam, K. M. (2014). Hepatoprotectiveeﬀect of polyphenolsrichmethanolic extract of Amorphophallus commutatus var. wayanadensisagainst CCl4 induced hepaticinjury in swissalbinomice. Food Chem. Toxicol. 67, 105–112. doi: 10.1016/j.fct.2014. 02.028.
[11]	Hegde K., A. B. Joshia. (2009). Hepatoprotective effect of Carissa carandas root extract against CCl4 and paracetamol induced hepatic oxidative stress. India J. Exp. Biol., 47: 660-667.
[12]	Suresh K. S. V. & Mishra S. H. (2008). Hepatoprotective effect of Pergulariadaemia (Forsk.) ethanol extract and it fraction. Indian. J. Exp. Biol. 46(6): 447-452.
[13]	Krishna K. L., Mruthunjaya K., Patel J. A. (2010). Antioxidant and hepatoprotective potential of stem methanolic extract of JusticiagendarussaBurm. Int. J. Pharma. 67: 2-80.
[14]	Wegner S. T&Fintelmamann V. (1999). Pharmacological properties and therapeutic profile of artichoke (Cynarascolymus L.). Wien Med Wochenschr. 149: 241-247.

Cite This Article

Plain Text BibTeX RIS

APA Style

Miezan Bile Aka Patrice, Kouakou Sylvain Landry, Droucoula Guillaume Cyril, Kouakou-Syransy Giselle, Koffi Akissi Jeanne, et al. (2017). Evaluation of Hepatoprotective Activities Aqueous and Hydroethanolic Extracts from the Leaves of Erythrococca anomala in Rats. Advances in Biochemistry, 5(4), 61-66. https://doi.org/10.11648/j.ab.20170504.12

Copy | Download

ACS Style

Miezan Bile Aka Patrice; Kouakou Sylvain Landry; Droucoula Guillaume Cyril; Kouakou-Syransy Giselle; Koffi Akissi Jeanne, et al. Evaluation of Hepatoprotective Activities Aqueous and Hydroethanolic Extracts from the Leaves of Erythrococca anomala in Rats. Adv. Biochem. 2017, 5(4), 61-66. doi: 10.11648/j.ab.20170504.12

Copy | Download

AMA Style

Miezan Bile Aka Patrice, Kouakou Sylvain Landry, Droucoula Guillaume Cyril, Kouakou-Syransy Giselle, Koffi Akissi Jeanne, et al. Evaluation of Hepatoprotective Activities Aqueous and Hydroethanolic Extracts from the Leaves of Erythrococca anomala in Rats. Adv Biochem. 2017;5(4):61-66. doi: 10.11648/j.ab.20170504.12

Copy | Download

@article{10.11648/j.ab.20170504.12,
  author = {Miezan Bile Aka Patrice and Kouakou Sylvain Landry and Droucoula Guillaume Cyril and Kouakou-Syransy Giselle and Koffi Akissi Jeanne and Yapi Houphouet Felix},
  title = {Evaluation of Hepatoprotective Activities Aqueous and Hydroethanolic Extracts from the Leaves of Erythrococca anomala in Rats},
  journal = {Advances in Biochemistry},
  volume = {5},
  number = {4},
  pages = {61-66},
  doi = {10.11648/j.ab.20170504.12},
  url = {https://doi.org/10.11648/j.ab.20170504.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ab.20170504.12},
  abstract = {Widely used in Ivory Coast and sub-Saharan Africa in traditional medicine as anti-inflammatory, antioxidant, laxative and purgative, Erythrococca anomala (Euphorbiaceae), is an annual plant. In this work, the aim was to evaluate the Hepatoprotective activity of the aqueous and hydroethanolic extracts of the leaves. Carbon Tetrachloride (CCl4) was used to induce hepatotoxicity in rats. This hepatotoxic effect causes a significant increase in the levels of liver enzymes and serum proteins. Hepatoprotective activity was assessed by assaying liver enzymes such as serum transaminases (alanine aminotransferase and aspartate aminotransferase) and proteins by spectrophotometric and electrophoretic techniques. When administered orally as a preventive and curative dose of 100 and 200 mg / kg body weight, the extracts significantly reduce significantly as silymarin, the reference hepatoprotective substance at 100 mg / kg bw, hepatotoxicity induced by Carbon tetrachloride (CCL4) at a dose of 5 mg / kg bw after a significant reduction in liver enzyme levels, globulins and a significant increase in albumin. The possible mechanism of this hepatoprotective activity of the extracts may be due to the action of the antioxidants such as the flavonoids, the phenols present in the extracts.},
 year = {2017}
}

Copy | Download

TY  - JOUR
T1  - Evaluation of Hepatoprotective Activities Aqueous and Hydroethanolic Extracts from the Leaves of Erythrococca anomala in Rats
AU  - Miezan Bile Aka Patrice
AU  - Kouakou Sylvain Landry
AU  - Droucoula Guillaume Cyril
AU  - Kouakou-Syransy Giselle
AU  - Koffi Akissi Jeanne
AU  - Yapi Houphouet Felix
Y1  - 2017/07/13
PY  - 2017
N1  - https://doi.org/10.11648/j.ab.20170504.12
DO  - 10.11648/j.ab.20170504.12
T2  - Advances in Biochemistry
JF  - Advances in Biochemistry
JO  - Advances in Biochemistry
SP  - 61
EP  - 66
PB  - Science Publishing Group
SN  - 2329-0862
UR  - https://doi.org/10.11648/j.ab.20170504.12
AB  - Widely used in Ivory Coast and sub-Saharan Africa in traditional medicine as anti-inflammatory, antioxidant, laxative and purgative, Erythrococca anomala (Euphorbiaceae), is an annual plant. In this work, the aim was to evaluate the Hepatoprotective activity of the aqueous and hydroethanolic extracts of the leaves. Carbon Tetrachloride (CCl4) was used to induce hepatotoxicity in rats. This hepatotoxic effect causes a significant increase in the levels of liver enzymes and serum proteins. Hepatoprotective activity was assessed by assaying liver enzymes such as serum transaminases (alanine aminotransferase and aspartate aminotransferase) and proteins by spectrophotometric and electrophoretic techniques. When administered orally as a preventive and curative dose of 100 and 200 mg / kg body weight, the extracts significantly reduce significantly as silymarin, the reference hepatoprotective substance at 100 mg / kg bw, hepatotoxicity induced by Carbon tetrachloride (CCL4) at a dose of 5 mg / kg bw after a significant reduction in liver enzyme levels, globulins and a significant increase in albumin. The possible mechanism of this hepatoprotective activity of the extracts may be due to the action of the antioxidants such as the flavonoids, the phenols present in the extracts.
VL  - 5
IS  - 4
ER  -

Copy | Download

Author Information

Miezan Bile Aka Patrice

Laboratory of Biochemical Pharmacodynamics, UFR Biosciences, Felix Houphouet Boigny University, Abidjan, Ivory Coast
Kouakou Sylvain Landry

Department of Pharmacology, Clinical and Therapeutic Pharmacy UFR Pharmaceutical and Biological Sciences, Felix Houphouet Boigny University, Abidjan, Ivory Coast
Droucoula Guillaume Cyril

Laboratory of Biochemical Pharmacodynamics, UFR Biosciences, Felix Houphouet Boigny University, Abidjan, Ivory Coast
Kouakou-Syransy Giselle

Department of Pharmacology, Clinical and Therapeutic Pharmacy UFR Pharmaceutical and Biological Sciences, Felix Houphouet Boigny University, Abidjan, Ivory Coast
Koffi Akissi Jeanne

Laboratory of Biochemical Pharmacodynamics, UFR Biosciences, Felix Houphouet Boigny University, Abidjan, Ivory Coast
Yapi Houphouet Felix

Laboratory of Biochemical Pharmacodynamics, UFR Biosciences, Felix Houphouet Boigny University, Abidjan, Ivory Coast

Download PDF

Sections

Plain Text BibTeX RIS

APA Style

Miezan Bile Aka Patrice, Kouakou Sylvain Landry, Droucoula Guillaume Cyril, Kouakou-Syransy Giselle, Koffi Akissi Jeanne, et al. (2017). Evaluation of Hepatoprotective Activities Aqueous and Hydroethanolic Extracts from the Leaves of Erythrococca anomala in Rats. Advances in Biochemistry, 5(4), 61-66. https://doi.org/10.11648/j.ab.20170504.12

Copy | Download

ACS Style

Miezan Bile Aka Patrice; Kouakou Sylvain Landry; Droucoula Guillaume Cyril; Kouakou-Syransy Giselle; Koffi Akissi Jeanne, et al. Evaluation of Hepatoprotective Activities Aqueous and Hydroethanolic Extracts from the Leaves of Erythrococca anomala in Rats. Adv. Biochem. 2017, 5(4), 61-66. doi: 10.11648/j.ab.20170504.12

Copy | Download

AMA Style

Miezan Bile Aka Patrice, Kouakou Sylvain Landry, Droucoula Guillaume Cyril, Kouakou-Syransy Giselle, Koffi Akissi Jeanne, et al. Evaluation of Hepatoprotective Activities Aqueous and Hydroethanolic Extracts from the Leaves of Erythrococca anomala in Rats. Adv Biochem. 2017;5(4):61-66. doi: 10.11648/j.ab.20170504.12

Copy | Download

@article{10.11648/j.ab.20170504.12,
  author = {Miezan Bile Aka Patrice and Kouakou Sylvain Landry and Droucoula Guillaume Cyril and Kouakou-Syransy Giselle and Koffi Akissi Jeanne and Yapi Houphouet Felix},
  title = {Evaluation of Hepatoprotective Activities Aqueous and Hydroethanolic Extracts from the Leaves of Erythrococca anomala in Rats},
  journal = {Advances in Biochemistry},
  volume = {5},
  number = {4},
  pages = {61-66},
  doi = {10.11648/j.ab.20170504.12},
  url = {https://doi.org/10.11648/j.ab.20170504.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ab.20170504.12},
  abstract = {Widely used in Ivory Coast and sub-Saharan Africa in traditional medicine as anti-inflammatory, antioxidant, laxative and purgative, Erythrococca anomala (Euphorbiaceae), is an annual plant. In this work, the aim was to evaluate the Hepatoprotective activity of the aqueous and hydroethanolic extracts of the leaves. Carbon Tetrachloride (CCl4) was used to induce hepatotoxicity in rats. This hepatotoxic effect causes a significant increase in the levels of liver enzymes and serum proteins. Hepatoprotective activity was assessed by assaying liver enzymes such as serum transaminases (alanine aminotransferase and aspartate aminotransferase) and proteins by spectrophotometric and electrophoretic techniques. When administered orally as a preventive and curative dose of 100 and 200 mg / kg body weight, the extracts significantly reduce significantly as silymarin, the reference hepatoprotective substance at 100 mg / kg bw, hepatotoxicity induced by Carbon tetrachloride (CCL4) at a dose of 5 mg / kg bw after a significant reduction in liver enzyme levels, globulins and a significant increase in albumin. The possible mechanism of this hepatoprotective activity of the extracts may be due to the action of the antioxidants such as the flavonoids, the phenols present in the extracts.},
 year = {2017}
}

Copy | Download

TY  - JOUR
T1  - Evaluation of Hepatoprotective Activities Aqueous and Hydroethanolic Extracts from the Leaves of Erythrococca anomala in Rats
AU  - Miezan Bile Aka Patrice
AU  - Kouakou Sylvain Landry
AU  - Droucoula Guillaume Cyril
AU  - Kouakou-Syransy Giselle
AU  - Koffi Akissi Jeanne
AU  - Yapi Houphouet Felix
Y1  - 2017/07/13
PY  - 2017
N1  - https://doi.org/10.11648/j.ab.20170504.12
DO  - 10.11648/j.ab.20170504.12
T2  - Advances in Biochemistry
JF  - Advances in Biochemistry
JO  - Advances in Biochemistry
SP  - 61
EP  - 66
PB  - Science Publishing Group
SN  - 2329-0862
UR  - https://doi.org/10.11648/j.ab.20170504.12
AB  - Widely used in Ivory Coast and sub-Saharan Africa in traditional medicine as anti-inflammatory, antioxidant, laxative and purgative, Erythrococca anomala (Euphorbiaceae), is an annual plant. In this work, the aim was to evaluate the Hepatoprotective activity of the aqueous and hydroethanolic extracts of the leaves. Carbon Tetrachloride (CCl4) was used to induce hepatotoxicity in rats. This hepatotoxic effect causes a significant increase in the levels of liver enzymes and serum proteins. Hepatoprotective activity was assessed by assaying liver enzymes such as serum transaminases (alanine aminotransferase and aspartate aminotransferase) and proteins by spectrophotometric and electrophoretic techniques. When administered orally as a preventive and curative dose of 100 and 200 mg / kg body weight, the extracts significantly reduce significantly as silymarin, the reference hepatoprotective substance at 100 mg / kg bw, hepatotoxicity induced by Carbon tetrachloride (CCL4) at a dose of 5 mg / kg bw after a significant reduction in liver enzyme levels, globulins and a significant increase in albumin. The possible mechanism of this hepatoprotective activity of the extracts may be due to the action of the antioxidants such as the flavonoids, the phenols present in the extracts.
VL  - 5
IS  - 4
ER  -

Copy | Download