Introduction: Eribroma oblongum (Malvaceae) (EO) stem bark is also used in traditional medicine to treat cardiovascular diseases and painful menstruation. This study aimed to estimate the mineral composition, in vitro antioxidant properties, and Interleukin-17 (IL-17) expression levels of hydroethanolic extract (EOHE) and the powder fraction ˂ 125µm (EOP) of Eribroma oblongum (EO), in Wistar rats with atherogenic diet-induced steatotic liver. Materials and methods: The mineral composition of these therapeutic plants was characterized through atomic absorption spectrometry. Antioxidant activities were measured using spectroscopic methods. Rats were given daily doses of EOP, EOHE (100mg/kg), or Atorvastatin (10mg/kg) as a positive control for 45 days at the same time as being fed an atherogenic diet. At the end of the treatment period, animals were sacrificed and liver and blood were collected for evaluation of biochemical parameters. The serum levels of cytokines IL-17 were measured by ELISA. Results: The results showed that EOP had a significantly P˂0.0001 higher composition of Iron (25.75±0.6464g/100g DM), Zinc (11.71±7164g/100g DM), Vitamin C (41.60 ± 4.864g/100g DM), Manganese (2.02±0.0164g/100g DM), Selenium (0.037±0.000764g/100g DM) than EOHE. EOHE had a significantly higher polyphenol content (p<0.05) than EOP 917.33±23.63µg CaE/g DM. EOP and EOHE had good polyphenol contents (in descending order EOHE (995.43±24.10 (µg CaE/g DM)) > EOP(917.33±23.63 (µg CaE/g DM)). DPPH, FRAP, and TAC increased positively with hydroethanolic extract and powder concentration. EOP significantly (P˂0.0001) reduced IL-17 expression by 48,76% compared with the negative control group. Conclusion: These results demonstrated that EOP can be a source of anti-inflammatory agents in the fight against metabolic diseases. The therapeutic potential of Eribroma oblongum for the treatment of steatosis could be considered in further studies.
| Published in | Advances in Biochemistry (Volume 12, Issue 1) |
| DOI | 10.11648/j.ab.20241201.12 |
| Page(s) | 10-19 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2024. Published by Science Publishing Group |
Eribroma oblongum, Mineral Composition, Antioxidant Properties, Expression of Interleukin-17
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APA Style
Valentine, T. M., Jacky, N. B., Alphonse, S. T., Lanvin, E. E. F., Steve, N. S. F., et al. (2024). In Vitro Antioxidant Properties and Interleukin-17 Expression Levels of Eribroma oblongum (Malvaceae) in Wistar Rats with Atherogenic Diet-Induced Steatotic Liver. Advances in Biochemistry, 12(1), 10-19. https://doi.org/10.11648/j.ab.20241201.12
ACS Style
Valentine, T. M.; Jacky, N. B.; Alphonse, S. T.; Lanvin, E. E. F.; Steve, N. S. F., et al. In Vitro Antioxidant Properties and Interleukin-17 Expression Levels of Eribroma oblongum (Malvaceae) in Wistar Rats with Atherogenic Diet-Induced Steatotic Liver. Adv. Biochem. 2024, 12(1), 10-19. doi: 10.11648/j.ab.20241201.12
AMA Style
Valentine TM, Jacky NB, Alphonse ST, Lanvin EEF, Steve NSF, et al. In Vitro Antioxidant Properties and Interleukin-17 Expression Levels of Eribroma oblongum (Malvaceae) in Wistar Rats with Atherogenic Diet-Induced Steatotic Liver. Adv Biochem. 2024;12(1):10-19. doi: 10.11648/j.ab.20241201.12
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Download@article{10.11648/j.ab.20241201.12,
author = {Tsague Marthe Valentine and Njiki Bikoï Jacky and Sokamte Tegang Alphonse and Edoun Ebouel Ferdinand Lanvin and Nodem Sohanang Francky Steve and Ahamat Abakar and Mahoumo Fodop Anaelle Orel and M’bann Nsonngan Salomon and Ze Minkande Jacqueline},
title = {In Vitro Antioxidant Properties and Interleukin-17 Expression Levels of Eribroma oblongum (Malvaceae) in Wistar Rats with Atherogenic Diet-Induced Steatotic Liver},
journal = {Advances in Biochemistry},
volume = {12},
number = {1},
pages = {10-19},
doi = {10.11648/j.ab.20241201.12},
url = {https://doi.org/10.11648/j.ab.20241201.12},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ab.20241201.12},
abstract = {Introduction: Eribroma oblongum (Malvaceae) (EO) stem bark is also used in traditional medicine to treat cardiovascular diseases and painful menstruation. This study aimed to estimate the mineral composition, in vitro antioxidant properties, and Interleukin-17 (IL-17) expression levels of hydroethanolic extract (EOHE) and the powder fraction ˂ 125µm (EOP) of Eribroma oblongum (EO), in Wistar rats with atherogenic diet-induced steatotic liver. Materials and methods: The mineral composition of these therapeutic plants was characterized through atomic absorption spectrometry. Antioxidant activities were measured using spectroscopic methods. Rats were given daily doses of EOP, EOHE (100mg/kg), or Atorvastatin (10mg/kg) as a positive control for 45 days at the same time as being fed an atherogenic diet. At the end of the treatment period, animals were sacrificed and liver and blood were collected for evaluation of biochemical parameters. The serum levels of cytokines IL-17 were measured by ELISA. Results: The results showed that EOP had a significantly P˂0.0001 higher composition of Iron (25.75±0.6464g/100g DM), Zinc (11.71±7164g/100g DM), Vitamin C (41.60 ± 4.864g/100g DM), Manganese (2.02±0.0164g/100g DM), Selenium (0.037±0.000764g/100g DM) than EOHE. EOHE had a significantly higher polyphenol content (p) than EOP 917.33±23.63µg CaE/g DM. EOP and EOHE had good polyphenol contents (in descending order EOHE (995.43±24.10 (µg CaE/g DM)) > EOP(917.33±23.63 (µg CaE/g DM)). DPPH, FRAP, and TAC increased positively with hydroethanolic extract and powder concentration. EOP significantly (P˂0.0001) reduced IL-17 expression by 48,76% compared with the negative control group. Conclusion: These results demonstrated that EOP can be a source of anti-inflammatory agents in the fight against metabolic diseases. The therapeutic potential of Eribroma oblongum for the treatment of steatosis could be considered in further studies.
},
year = {2024}
}
TY - JOUR T1 - In Vitro Antioxidant Properties and Interleukin-17 Expression Levels of Eribroma oblongum (Malvaceae) in Wistar Rats with Atherogenic Diet-Induced Steatotic Liver AU - Tsague Marthe Valentine AU - Njiki Bikoï Jacky AU - Sokamte Tegang Alphonse AU - Edoun Ebouel Ferdinand Lanvin AU - Nodem Sohanang Francky Steve AU - Ahamat Abakar AU - Mahoumo Fodop Anaelle Orel AU - M’bann Nsonngan Salomon AU - Ze Minkande Jacqueline Y1 - 2024/01/23 PY - 2024 N1 - https://doi.org/10.11648/j.ab.20241201.12 DO - 10.11648/j.ab.20241201.12 T2 - Advances in Biochemistry JF - Advances in Biochemistry JO - Advances in Biochemistry SP - 10 EP - 19 PB - Science Publishing Group SN - 2329-0862 UR - https://doi.org/10.11648/j.ab.20241201.12 AB - Introduction: Eribroma oblongum (Malvaceae) (EO) stem bark is also used in traditional medicine to treat cardiovascular diseases and painful menstruation. This study aimed to estimate the mineral composition, in vitro antioxidant properties, and Interleukin-17 (IL-17) expression levels of hydroethanolic extract (EOHE) and the powder fraction ˂ 125µm (EOP) of Eribroma oblongum (EO), in Wistar rats with atherogenic diet-induced steatotic liver. Materials and methods: The mineral composition of these therapeutic plants was characterized through atomic absorption spectrometry. Antioxidant activities were measured using spectroscopic methods. Rats were given daily doses of EOP, EOHE (100mg/kg), or Atorvastatin (10mg/kg) as a positive control for 45 days at the same time as being fed an atherogenic diet. At the end of the treatment period, animals were sacrificed and liver and blood were collected for evaluation of biochemical parameters. The serum levels of cytokines IL-17 were measured by ELISA. Results: The results showed that EOP had a significantly P˂0.0001 higher composition of Iron (25.75±0.6464g/100g DM), Zinc (11.71±7164g/100g DM), Vitamin C (41.60 ± 4.864g/100g DM), Manganese (2.02±0.0164g/100g DM), Selenium (0.037±0.000764g/100g DM) than EOHE. EOHE had a significantly higher polyphenol content (p) than EOP 917.33±23.63µg CaE/g DM. EOP and EOHE had good polyphenol contents (in descending order EOHE (995.43±24.10 (µg CaE/g DM)) > EOP(917.33±23.63 (µg CaE/g DM)). DPPH, FRAP, and TAC increased positively with hydroethanolic extract and powder concentration. EOP significantly (P˂0.0001) reduced IL-17 expression by 48,76% compared with the negative control group. Conclusion: These results demonstrated that EOP can be a source of anti-inflammatory agents in the fight against metabolic diseases. The therapeutic potential of Eribroma oblongum for the treatment of steatosis could be considered in further studies. VL - 12 IS - 1 ER -
Department of Biomedical Sciences, University of Ngaoundere, Ngaoundere, Cameroon
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