Artemisia afra (Jacq. Ex. Wild), or "African Wormwood" belonging to the family of Astereaces and is widely used traditionally for health care in the eastern part of Africa with few research evidence substantiating its safety. The aim of this study was to investigate the safety of the ethanolic, dichloromethane, and hexanolic extracts of Artemisia afra by determining its pharmaco-toxicological effects after an acute oral administration in mice and to test also their in vivo antimalarial effects. Oral acute doses of Artemisia afra extracts were given to thirty mice at the doses of 1000, 2000 and 2500 mg/kg of body weight. The mice were then observed for fourteen days, toxicity signs, body weight, organs weight and biochemical parameters were checked. Four days peter’s test was run on mice to determine the in vivo antimalarial activity of the plant extracts and the IC50 for each extract was determined. The results show few toxicity signs from the first two days after oral administration. There were no differences in organs weight and body weight for the experimental mice when compared to the control group. The level of alanine transaminase (ALT) and aspartate transaminase (AST) were found do not be statistically different from the control. The LD50 of the extracts was found to be greater than 2500 mg/kg of body weight. The results also showed a high antimalarial effect of the extracts when tested in vivo using Plasmodium Berghei Anka. In Conclusion Artemisia afra is a strong drug candidate for malaria with no toxic effects in high dosage.
| Published in | Advances in Bioscience and Bioengineering (Volume 7, Issue 4) |
| DOI | 10.11648/j.abb.20190704.12 |
| Page(s) | 64-71 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2019. Published by Science Publishing Group |
Oral Acute Toxicity, Medicinal Plant, Antimalarial Assay, Plasmodium Berghei Anka, Artemisia afra, Biochemical Test
| [1] | Spies L, Koekemoer TC, Sowemimo AA, Goosen ED, Van de Venter M. Caspase-dependent apoptosis is induced by Artemisia afra Jacq. Ex Willd in a mitochondria-dependent manner after G2/M arrest. South African Journal of Botany; 2013; 84 104-109. |
| [2] | Jamshidi-Kia F, Lorigooini Z, Amini-Khoei H. Medicinal plants: Past history and future perspective. Journal of Herbmed Pharmacology. 2018; 7 (1): 1-7. |
| [3] | Gurib-Fakim, A.,. Medicinal plants: traditions of yesterday and drugs of tomorrow. Molecular Aspects of Medicine 2006, 27, 1–93. |
| [4] | Gayathri V. Patil, Sujata K. Dass and Ramesh Chandra,’’ Artemisia afra and Modern Diseases’’ Journal of Pharmacogenomics & Pharmacoproteomics, 2011, 2: 3 DOI: 10.4172/2153-0645.1000105. |
| [5] | Liu, N. Q., Van der Kooy, F., Verpoorte, R.,. Artemisia afra: a potential flagship for African medicinal plants? South African Journal of Botany 2009; 75, 185–195. |
| [6] | Harris, L., An evaluation of the bronchodilator properties of Mentha Longifolia and Artemisia afra, traditional medicinal plants used in the Western Cape M. Thesis, Discipline of pharmacology. School of pharmacy, University of the Western Cape. Bellville. 2002. |
| [7] | Cunningham, A., et al., Zulu Medicinal Plants: An inventory. South Africa, University of Natal press. Scottsville, 1996; p: 327. |
| [8] | MRC and S. Health info, Traditional medicines database: www.mrc.ac.za/Tramed3/Tramed3PlantPharmacologyDetails.2004. |
| [9] | Moges, K., et al., In Vitro Test of Five Ethiopian Medicinal Plants for Antimalarial activity against plasmodium Falciparum. Ethiopian J. Sci 1998; 21 (1): p. 81-89. |
| [10] | James W. Gathirwa, Geoffrey M. Rukunga, Eliud N. M. Njagi, Sabah A. Omar, Anastasia N. Guantai, Charles N. Muthaura, Peter G. Mwitari, Cecilia W. Kimani, Peter G. Kirira, Festus M. Tolo, Teresia N. Ndunda, Isaiah O. Ndiege, In vitro anti-plasmodial and in vivo anti-malarial activity of some plants traditionally used for the treatment of malaria by the Meru community in Kenya; The Japanese Society of Pharmacognosy and Springer 2007; DOI: 10.1007/s11418-007-0140-0. |
| [11] | Fennell, C. W., et al., Assessing African medicinal plants for efficacy and safety: pharmacological screening and Toxicology. Journal of Ethno-pharmacology. 2004; 94 (2-3): p. 205-217. |
| [12] | OECD Guideline for testing of chemicals; ‘’Acute oral toxicity-Acute toxic class method’’, 2001, PDF available at https://ntp.niehs.nih.gov/iccvam/suppdocs/feddocs/oecd/oecd_gl423.pdf |
| [13] | Peters W, Portus JH, Robinson BL. ‘’The chemotherapy of rodent malaria, XXII. The value of drug-resistant strains of Plasmodium berghei in screening for blood schizontocidal activity.’’ Ann Trop Med Parasitol, 1975; 69: 155–171 |
| [14] | Idris Ahmed Issa and Mohammed Hussen Bule. Hypoglycemic Effect of Aqueous and Methanolic Extract of Artemisia afraon Alloxan Induced Diabetic Swiss Albino Mice. Journal Evid Based Complement Alternat Med. 2015; 2015: 752486. |
| [15] | Hilaly, J. E., Z. H. Israeli, and B. Lyoussi, Acute and chronic toxicological studies of Ajuga Ivain experimental animals. J. Ethno-pharmacology, 2004; 91: p. 43-50. |
| [16] | Hermine Boukeng Jatsa, Joseph Bertin Kadji Fassi, Mérimé Christian Kenfack, Nestor Gipwe Feussom, Mireille Poumeni Kameni, Nadège Distele Simo, Emilienne Tienga Nkondo, Alain Bertrand Dongmo, Louis-Albert Tchuem Tchuent.’ Acute and sub-chronic oral toxicity studies of the leaves aqueous extract of Clerodendrum umbellatum Poir. on mice’. American journal of physiology, biochemistry and pharmacology, 2018 vol 7, no. 2, page 75–85 10.5455 |
| [17] | Teo S, Stirling D, Thomas S, Hoberman A, Kiorpes A, Khetani V. A 90-day oral gavage toxicity study of D-methylphenidate and D, L-methylphenidate in Sprague Dawley rats. Toxicology 2002; 179: 183-196. |
| [18] | Heywood, Long term toxicity. In: Balls M, Riddell RJ, and Worden AN, editors, Animals and alternatives in toxicity testing, London: Academic Press. 1983; p. 79-89. |
| [19] | Nikodimos Eshetu, Mekbeb Afework, Eyasu Makonnen, Asfaw Debella, Wondwossen Ergete, Tesfaye Tolesssa. “Evaluation of the Acute and Sub-chronic Toxic Effects of Aqueous Leaf Extracts of Artemisia afra on Liver, Kidney and Some Blood Parameters in Wistar Rats” Advances in Bioscience and Bioengineering 2016; 1 (1): 1-9. |
| [20] | Amresh G, Singh PN, Rao CV (2008). Toxicological screening of traditional medicine Laghupatha (Cissampelos pareira) in experimental animals. Journal of Ethnopharmacology 2008; 116: 454-460. |
| [21] | Nisha A, Muthukumar SP, Venkateswaran G. Safety evaluation of arachidonic acid rich Mortierella Alpina biomass in albino rats-a subchronic study. Regulatory Toxicology and Pharmacology 2009; 53: 186-194. |
| [22] | Pfeiffer CJ. A mathematical evaluation of the thymic weight parameter. Toxicology and Applied Pharmacology 1968; 13: 220-227. |
| [23] | Aniekan Imo Peter, Edwin CS Naidu, Edidiong Akang, Oluwatosin O Ogedengbe, Ugochukwu Offor, Sanjeev Rambharose, Rahul Kalhapure, Anil Chuturgoon, Thirumala Govender, and Onyemaechi O Azu. ‘Investigating Organ Toxicity Profile of Tenofovir and Tenofovir Nanoparticle on the Liver and Kidney: Experimental Animal Study’. Toxicol Res. 2018 Jul; 34 (3): 221–229. Published online 2018 Jul 15. doi: 10.5487/TR.2018.34.3.221. |
| [24] | Brautbar N, Williams IIJ. Industrial solvents and liver toxicity: risk assessment, risk factors and mechanisms. International Journal of Hygiene and Environmental Health 2002; 205: 479-491. |
| [25] | Kwo, Paul Y MD, FACG, FAASLD; Cohen, Stanley M MD, FACG, FAASLD; Lim, Joseph K MD, FACG, FAASLD.’ ACG Clinical Guideline: Evaluation of Abnormal Liver Chemistries’ American Journal of Gastroenterology: January 2017 - Volume 112 - Issue 1 - p 18–35. doi: 10.1038/ajg.2016.517 |
| [26] | S. Chanda, J. Parekh, Y. Vaghasiya, R. Dave, Y. Baravalia and R. Nair, medicinal plants - from traditional use to toxicity assessment: a review, international journal of pharmaceutical sciences and research, 2017. |
| [27] | Siyabulela Nutella, Pete Smitha, Lungile Matikab, James Mukunda, Hiram Arends, Nasiema Allieb, D. Mark Estes, Wilfred Mabuselae, Peter Folba, Lafras Steyn, Quinton Johnson, William R. Folk, James Synced, e, and Muazzam Jacobs, ‘’ efficacy of Artemisia afra phytotherapy in experimental tuberculosis’’, Elsevier Tuberculosis (Edinb). 2009 December; 89 (Suppl 1): S33–S40. doi: 10.1016/S1472-9792(09)70009-5. |
| [28] | Idris Ahmed Issa and Mohammed Hussein Bule,” Hypoglycemic Effect of Aqueous and Methanolic Extract of Artemisia afra on Alloxan-Induced Diabetic Swiss Albino Mice’’, Hindawi Publishing Corporation Evidence-Based Complementary and Alternative Medicine Volume 2015, Article ID 752486, 5 pages http://dx.doi.org/10.1155/2015/752486. |
APA Style
Ndeye Fatou Kane, Mutinda Cleophas Kyama, Joseph Kangethe Nganga, Ahmed Hassanali, Mouhamadou Diallo, et al. (2019). Acute Toxicity Effect of Artemisia Afra Plant Extracts on the Liver, Kidney, Spleen and in Vivo Antimalarial Assay on Swiss Albino Mice. Advances in Bioscience and Bioengineering, 7(4), 64-71. https://doi.org/10.11648/j.abb.20190704.12
ACS Style
Ndeye Fatou Kane; Mutinda Cleophas Kyama; Joseph Kangethe Nganga; Ahmed Hassanali; Mouhamadou Diallo, et al. Acute Toxicity Effect of Artemisia Afra Plant Extracts on the Liver, Kidney, Spleen and in Vivo Antimalarial Assay on Swiss Albino Mice. Adv. BioSci. Bioeng. 2019, 7(4), 64-71. doi: 10.11648/j.abb.20190704.12
AMA Style
Ndeye Fatou Kane, Mutinda Cleophas Kyama, Joseph Kangethe Nganga, Ahmed Hassanali, Mouhamadou Diallo, et al. Acute Toxicity Effect of Artemisia Afra Plant Extracts on the Liver, Kidney, Spleen and in Vivo Antimalarial Assay on Swiss Albino Mice. Adv BioSci Bioeng. 2019;7(4):64-71. doi: 10.11648/j.abb.20190704.12
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Download@article{10.11648/j.abb.20190704.12,
author = {Ndeye Fatou Kane and Mutinda Cleophas Kyama and Joseph Kangethe Nganga and Ahmed Hassanali and Mouhamadou Diallo and Francis Thuo Kimani},
title = {Acute Toxicity Effect of Artemisia Afra Plant Extracts on the Liver, Kidney, Spleen and in Vivo Antimalarial Assay on Swiss Albino Mice},
journal = {Advances in Bioscience and Bioengineering},
volume = {7},
number = {4},
pages = {64-71},
doi = {10.11648/j.abb.20190704.12},
url = {https://doi.org/10.11648/j.abb.20190704.12},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.abb.20190704.12},
abstract = {Artemisia afra (Jacq. Ex. Wild), or "African Wormwood" belonging to the family of Astereaces and is widely used traditionally for health care in the eastern part of Africa with few research evidence substantiating its safety. The aim of this study was to investigate the safety of the ethanolic, dichloromethane, and hexanolic extracts of Artemisia afra by determining its pharmaco-toxicological effects after an acute oral administration in mice and to test also their in vivo antimalarial effects. Oral acute doses of Artemisia afra extracts were given to thirty mice at the doses of 1000, 2000 and 2500 mg/kg of body weight. The mice were then observed for fourteen days, toxicity signs, body weight, organs weight and biochemical parameters were checked. Four days peter’s test was run on mice to determine the in vivo antimalarial activity of the plant extracts and the IC50 for each extract was determined. The results show few toxicity signs from the first two days after oral administration. There were no differences in organs weight and body weight for the experimental mice when compared to the control group. The level of alanine transaminase (ALT) and aspartate transaminase (AST) were found do not be statistically different from the control. The LD50 of the extracts was found to be greater than 2500 mg/kg of body weight. The results also showed a high antimalarial effect of the extracts when tested in vivo using Plasmodium Berghei Anka. In Conclusion Artemisia afra is a strong drug candidate for malaria with no toxic effects in high dosage.},
year = {2019}
}
TY - JOUR T1 - Acute Toxicity Effect of Artemisia Afra Plant Extracts on the Liver, Kidney, Spleen and in Vivo Antimalarial Assay on Swiss Albino Mice AU - Ndeye Fatou Kane AU - Mutinda Cleophas Kyama AU - Joseph Kangethe Nganga AU - Ahmed Hassanali AU - Mouhamadou Diallo AU - Francis Thuo Kimani Y1 - 2019/10/12 PY - 2019 N1 - https://doi.org/10.11648/j.abb.20190704.12 DO - 10.11648/j.abb.20190704.12 T2 - Advances in Bioscience and Bioengineering JF - Advances in Bioscience and Bioengineering JO - Advances in Bioscience and Bioengineering SP - 64 EP - 71 PB - Science Publishing Group SN - 2330-4162 UR - https://doi.org/10.11648/j.abb.20190704.12 AB - Artemisia afra (Jacq. Ex. Wild), or "African Wormwood" belonging to the family of Astereaces and is widely used traditionally for health care in the eastern part of Africa with few research evidence substantiating its safety. The aim of this study was to investigate the safety of the ethanolic, dichloromethane, and hexanolic extracts of Artemisia afra by determining its pharmaco-toxicological effects after an acute oral administration in mice and to test also their in vivo antimalarial effects. Oral acute doses of Artemisia afra extracts were given to thirty mice at the doses of 1000, 2000 and 2500 mg/kg of body weight. The mice were then observed for fourteen days, toxicity signs, body weight, organs weight and biochemical parameters were checked. Four days peter’s test was run on mice to determine the in vivo antimalarial activity of the plant extracts and the IC50 for each extract was determined. The results show few toxicity signs from the first two days after oral administration. There were no differences in organs weight and body weight for the experimental mice when compared to the control group. The level of alanine transaminase (ALT) and aspartate transaminase (AST) were found do not be statistically different from the control. The LD50 of the extracts was found to be greater than 2500 mg/kg of body weight. The results also showed a high antimalarial effect of the extracts when tested in vivo using Plasmodium Berghei Anka. In Conclusion Artemisia afra is a strong drug candidate for malaria with no toxic effects in high dosage. VL - 7 IS - 4 ER -
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