Determination of Ascorbic Acid in <i>Citrus Sinensis</i> and <i>Ananas Comosus</i> Using Poly (3, 4-Ethylenedioxythiophene) Modified Glassy Carbon Electrode

Amanial Haile Reda; Fisseha Guesh Gebremeskel

doi:doi:10.11648/j.ajac.20160401.11

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Determination of Ascorbic Acid in Citrus Sinensis and Ananas Comosus Using Poly (3, 4-Ethylenedioxythiophene) Modified Glassy Carbon Electrode

Amanial Haile Reda, Fisseha Guesh Gebremeskel

Published in American Journal of Applied Chemistry (Volume 4, Issue 1)

Received: 16 November 2015 Accepted: 26 November 2015 Published: 4 January 2016

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Abstract

This study describes the preparation and application of a poly (3, 4-Ethylenedioxythiophene) (PEDOT) modified glassy carbon electrode for ascorbic acid determination in fruit samples by square wave voltammetric method. The PEDOT modified electrode was prepared by electrochemical polymerizing of 3, 4-Ethylenedioxythiophene (EDOT) on a glassy carbon electrode in a non-aqueous medium (0.1 M Tetrabutylammoniumhexafluorophosphate and 0.1 M EDOT in acetonitrile). The best performance of the PEDOT modified electrode in 0.1 M phosphate buffer was obtained at pH 5.0. In addition to this other parameters like electrode thickness, square wave amplitude, and square wave frequency were optimized. Under these conditions, an oxidation potential of ascorbic acid was observed at 395.24 mV versus Ag/AgCl/ reference electrode. The square wave voltammetric study indicated that the PEDOT modified electrode showed a very good electrocatalytic activity by reducing the overpotential by 113.69 mV for ascorbic acid oxidation. The modified electrode also showed a linear response range between 50 and 90μM. The proposed method was checked by determining the recovery of ascorbic acid in two commercially available tablets and provided an excellent recovery (i.e. 99.43 and 93.80%). Finally the method was applied in Orange and Pineapple fruits and 68.11±0.012 and 45.46±0.023 mg ascorbic acid per 100 g of the sample was found in each fruits respectively.

Published in	American Journal of Applied Chemistry (Volume 4, Issue 1)
DOI	10.11648/j.ajac.20160401.11
Page(s)	1-7
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2016. Published by Science Publishing Group

Keywords

Ascorbic Acid, Glassy Carbon Electrode, PEDOT, Square Wave Voltammetry

References

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[2]	Steptoe A. and Perkins L., 2003. Psychological factors associated with fruit and vegetable intake and with biomarkers in adults from a Low income Neighborhood, Journal of American Psychological Association, Vol. 22, PP. 148 - 155.
[3]	Dumbrava D., Moldovan C., Raba D., Popa M., 2012. Comparative analysis of vitamin C content and antioxidant activity of some fruits extracts, Journal of Agroalimentary processes and Technologies, Vol.18, PP. 223 - 228.
[4]	Tahirovic A., Copra – Janićijevic A, Basic N., Klepo L., Subasic M., 2012. Determination of vitamin C in flowers of some bosnian crataegus L. Species, PP. 1 - 12.
[5]	Shadrack D., Akwasi A., Felicia A., Nana A., Daniel G., Isaac K., Josephine K., 2015. Antioxidant potentials of tomato paste extracts found on major markets in Accra Metropolis. American Journal of Applied Chemistry. Vol. 3, PP. 158-163
[6]	Abubakar I., Mann A., Mathew J., 2015. Evaluation of phytochemical, anti-nutritional and antioxidant potentials of flower and seed methanol extracts of Senna alata L. Grown in Nigeria. American Journal of Applied Chemistry. Vol. 3. PP. 93-100.
[7]	Nojavan S., Khalilian F., Kiaie M. F., Rahimi A., Arabanian A., Chalavi S., 2008. Extraction and quantitative determination of ascorbic acid during different maturity stages of Rosa Canina L. fruit, Journal of Food Composition and Analysis, Vol. 21, PP. 300 - 305.
[8]	Ahmad H. and Alghamdi, 2010. Applications of stripping voltammetric techniques in food analysis, Arabian Journal of Chemistry, Vol. 3, PP. 1 - 7.
[9]	Yilmaz S., Sadikoglu M., Saglikoglu G., Yagmur S., Askin G., 2008. Determination of ascorbic acid in tablet dosage forms and some fruit juices by DPV, International Journal of electrochemical Science, Vol. 3, PP. 1534 - 1542.
[10]	Mehmet G. and Sibel A. O., 2011. The role of and the place of method validation in drug analysis using electroanalytical techniques, the Open Analytical Chemistry Journal, Vol. 5, PP. 1 - 21.
[11]	Magdalena A. P., Pop A., Petre G. N. and Pisoschi A., 2011. Determination of ascorbic acid content of some fruit juices and wine by Voltammetry performed at Pt and carbon paste electrodes, molecules, Vol.16, PP. 1349 - 1365.
[12]	Chandra U., Swamy B. E. K., Gilbert O. and Sherigara B.S., 2010. Determination of dopamine in presence of ascorbic acid at eriochrome black T modified carbon paste electrode: A voltammetric Study, International Journal of electrochemical science, Vol.5, PP.1475 - 1483.
[13]	Ngai K. S., Tan W. T., Zainal Z., Zawawi R. M., Zidan M., 2012. Electrochemical oxidation of ascorbic acid mediated by single-walled carbon nanotube/tungsten oxide nano particles modified glassy carbon electrodes, International Journal of Electrochemical Science, Vol. 7, PP. 4210 - 4222.
[14]	Mahmoud A. O., Osama H. A., Ahmed A. A. and Safaa F.S., 2013.Assay of amlodipine Besylate in tablets and human biological fluids by square wave adsorptive cathodic Stripping voltammetry, International Research Journal of Pure & Applied Chemistry Vol. 3, PP. 133 - 146.
[15]	Zhou C., Liu Z., Yan Y., Xusheng D., Yiu-Wing M. and Ringer S., 2011. Electro-synthesis of Novel nano structured PEDOT films and their application as catalyst support, Journal of Nano Scale Research, Vol. 6, PP. 2 - 6.
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APA Style

Amanial Haile Reda, Fisseha Guesh Gebremeskel. (2016). Determination of Ascorbic Acid in Citrus Sinensis and Ananas Comosus Using Poly (3, 4-Ethylenedioxythiophene) Modified Glassy Carbon Electrode. American Journal of Applied Chemistry, 4(1), 1-7. https://doi.org/10.11648/j.ajac.20160401.11

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ACS Style

Amanial Haile Reda; Fisseha Guesh Gebremeskel. Determination of Ascorbic Acid in Citrus Sinensis and Ananas Comosus Using Poly (3, 4-Ethylenedioxythiophene) Modified Glassy Carbon Electrode. Am. J. Appl. Chem. 2016, 4(1), 1-7. doi: 10.11648/j.ajac.20160401.11

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AMA Style

Amanial Haile Reda, Fisseha Guesh Gebremeskel. Determination of Ascorbic Acid in Citrus Sinensis and Ananas Comosus Using Poly (3, 4-Ethylenedioxythiophene) Modified Glassy Carbon Electrode. Am J Appl Chem. 2016;4(1):1-7. doi: 10.11648/j.ajac.20160401.11

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@article{10.11648/j.ajac.20160401.11,
  author = {Amanial Haile Reda and Fisseha Guesh Gebremeskel},
  title = {Determination of Ascorbic Acid in Citrus Sinensis and Ananas Comosus Using Poly (3, 4-Ethylenedioxythiophene) Modified Glassy Carbon Electrode},
  journal = {American Journal of Applied Chemistry},
  volume = {4},
  number = {1},
  pages = {1-7},
  doi = {10.11648/j.ajac.20160401.11},
  url = {https://doi.org/10.11648/j.ajac.20160401.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajac.20160401.11},
  abstract = {This study describes the preparation and application of a poly (3, 4-Ethylenedioxythiophene) (PEDOT) modified glassy carbon electrode for ascorbic acid determination in fruit samples by square wave voltammetric method. The PEDOT modified electrode was prepared by electrochemical polymerizing of 3, 4-Ethylenedioxythiophene (EDOT) on a glassy carbon electrode in a non-aqueous medium (0.1 M Tetrabutylammoniumhexafluorophosphate and 0.1 M EDOT in acetonitrile). The best performance of the PEDOT modified electrode in 0.1 M phosphate buffer was obtained at pH 5.0. In addition to this other parameters like electrode thickness, square wave amplitude, and square wave frequency were optimized. Under these conditions, an oxidation potential of ascorbic acid was observed at 395.24 mV versus Ag/AgCl/ reference electrode. The square wave voltammetric study indicated that the PEDOT modified electrode showed a very good electrocatalytic activity by reducing the overpotential by 113.69 mV for ascorbic acid oxidation. The modified electrode also showed a linear response range between 50 and 90μM. The proposed method was checked by determining the recovery of ascorbic acid in two commercially available tablets and provided an excellent recovery (i.e. 99.43 and 93.80%). Finally the method was applied in Orange and Pineapple fruits and 68.11±0.012 and 45.46±0.023 mg ascorbic acid per 100 g of the sample was found in each fruits respectively.},
 year = {2016}
}

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TY  - JOUR
T1  - Determination of Ascorbic Acid in Citrus Sinensis and Ananas Comosus Using Poly (3, 4-Ethylenedioxythiophene) Modified Glassy Carbon Electrode
AU  - Amanial Haile Reda
AU  - Fisseha Guesh Gebremeskel
Y1  - 2016/01/04
PY  - 2016
N1  - https://doi.org/10.11648/j.ajac.20160401.11
DO  - 10.11648/j.ajac.20160401.11
T2  - American Journal of Applied Chemistry
JF  - American Journal of Applied Chemistry
JO  - American Journal of Applied Chemistry
SP  - 1
EP  - 7
PB  - Science Publishing Group
SN  - 2330-8745
UR  - https://doi.org/10.11648/j.ajac.20160401.11
AB  - This study describes the preparation and application of a poly (3, 4-Ethylenedioxythiophene) (PEDOT) modified glassy carbon electrode for ascorbic acid determination in fruit samples by square wave voltammetric method. The PEDOT modified electrode was prepared by electrochemical polymerizing of 3, 4-Ethylenedioxythiophene (EDOT) on a glassy carbon electrode in a non-aqueous medium (0.1 M Tetrabutylammoniumhexafluorophosphate and 0.1 M EDOT in acetonitrile). The best performance of the PEDOT modified electrode in 0.1 M phosphate buffer was obtained at pH 5.0. In addition to this other parameters like electrode thickness, square wave amplitude, and square wave frequency were optimized. Under these conditions, an oxidation potential of ascorbic acid was observed at 395.24 mV versus Ag/AgCl/ reference electrode. The square wave voltammetric study indicated that the PEDOT modified electrode showed a very good electrocatalytic activity by reducing the overpotential by 113.69 mV for ascorbic acid oxidation. The modified electrode also showed a linear response range between 50 and 90μM. The proposed method was checked by determining the recovery of ascorbic acid in two commercially available tablets and provided an excellent recovery (i.e. 99.43 and 93.80%). Finally the method was applied in Orange and Pineapple fruits and 68.11±0.012 and 45.46±0.023 mg ascorbic acid per 100 g of the sample was found in each fruits respectively.
VL  - 4
IS  - 1
ER  -

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Author Information

Amanial Haile Reda

Department of Chemistry, College of Natural Sciences, Arbaminch University, Arbaminch, Ethiopia
Fisseha Guesh Gebremeskel

Department of Chemistry, College of Natural Sciences, Arbaminch University, Arbaminch, Ethiopia

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APA Style

Amanial Haile Reda, Fisseha Guesh Gebremeskel. (2016). Determination of Ascorbic Acid in Citrus Sinensis and Ananas Comosus Using Poly (3, 4-Ethylenedioxythiophene) Modified Glassy Carbon Electrode. American Journal of Applied Chemistry, 4(1), 1-7. https://doi.org/10.11648/j.ajac.20160401.11

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ACS Style

Amanial Haile Reda; Fisseha Guesh Gebremeskel. Determination of Ascorbic Acid in Citrus Sinensis and Ananas Comosus Using Poly (3, 4-Ethylenedioxythiophene) Modified Glassy Carbon Electrode. Am. J. Appl. Chem. 2016, 4(1), 1-7. doi: 10.11648/j.ajac.20160401.11

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AMA Style

Amanial Haile Reda, Fisseha Guesh Gebremeskel. Determination of Ascorbic Acid in Citrus Sinensis and Ananas Comosus Using Poly (3, 4-Ethylenedioxythiophene) Modified Glassy Carbon Electrode. Am J Appl Chem. 2016;4(1):1-7. doi: 10.11648/j.ajac.20160401.11

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@article{10.11648/j.ajac.20160401.11,
  author = {Amanial Haile Reda and Fisseha Guesh Gebremeskel},
  title = {Determination of Ascorbic Acid in Citrus Sinensis and Ananas Comosus Using Poly (3, 4-Ethylenedioxythiophene) Modified Glassy Carbon Electrode},
  journal = {American Journal of Applied Chemistry},
  volume = {4},
  number = {1},
  pages = {1-7},
  doi = {10.11648/j.ajac.20160401.11},
  url = {https://doi.org/10.11648/j.ajac.20160401.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajac.20160401.11},
  abstract = {This study describes the preparation and application of a poly (3, 4-Ethylenedioxythiophene) (PEDOT) modified glassy carbon electrode for ascorbic acid determination in fruit samples by square wave voltammetric method. The PEDOT modified electrode was prepared by electrochemical polymerizing of 3, 4-Ethylenedioxythiophene (EDOT) on a glassy carbon electrode in a non-aqueous medium (0.1 M Tetrabutylammoniumhexafluorophosphate and 0.1 M EDOT in acetonitrile). The best performance of the PEDOT modified electrode in 0.1 M phosphate buffer was obtained at pH 5.0. In addition to this other parameters like electrode thickness, square wave amplitude, and square wave frequency were optimized. Under these conditions, an oxidation potential of ascorbic acid was observed at 395.24 mV versus Ag/AgCl/ reference electrode. The square wave voltammetric study indicated that the PEDOT modified electrode showed a very good electrocatalytic activity by reducing the overpotential by 113.69 mV for ascorbic acid oxidation. The modified electrode also showed a linear response range between 50 and 90μM. The proposed method was checked by determining the recovery of ascorbic acid in two commercially available tablets and provided an excellent recovery (i.e. 99.43 and 93.80%). Finally the method was applied in Orange and Pineapple fruits and 68.11±0.012 and 45.46±0.023 mg ascorbic acid per 100 g of the sample was found in each fruits respectively.},
 year = {2016}
}

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TY  - JOUR
T1  - Determination of Ascorbic Acid in Citrus Sinensis and Ananas Comosus Using Poly (3, 4-Ethylenedioxythiophene) Modified Glassy Carbon Electrode
AU  - Amanial Haile Reda
AU  - Fisseha Guesh Gebremeskel
Y1  - 2016/01/04
PY  - 2016
N1  - https://doi.org/10.11648/j.ajac.20160401.11
DO  - 10.11648/j.ajac.20160401.11
T2  - American Journal of Applied Chemistry
JF  - American Journal of Applied Chemistry
JO  - American Journal of Applied Chemistry
SP  - 1
EP  - 7
PB  - Science Publishing Group
SN  - 2330-8745
UR  - https://doi.org/10.11648/j.ajac.20160401.11
AB  - This study describes the preparation and application of a poly (3, 4-Ethylenedioxythiophene) (PEDOT) modified glassy carbon electrode for ascorbic acid determination in fruit samples by square wave voltammetric method. The PEDOT modified electrode was prepared by electrochemical polymerizing of 3, 4-Ethylenedioxythiophene (EDOT) on a glassy carbon electrode in a non-aqueous medium (0.1 M Tetrabutylammoniumhexafluorophosphate and 0.1 M EDOT in acetonitrile). The best performance of the PEDOT modified electrode in 0.1 M phosphate buffer was obtained at pH 5.0. In addition to this other parameters like electrode thickness, square wave amplitude, and square wave frequency were optimized. Under these conditions, an oxidation potential of ascorbic acid was observed at 395.24 mV versus Ag/AgCl/ reference electrode. The square wave voltammetric study indicated that the PEDOT modified electrode showed a very good electrocatalytic activity by reducing the overpotential by 113.69 mV for ascorbic acid oxidation. The modified electrode also showed a linear response range between 50 and 90μM. The proposed method was checked by determining the recovery of ascorbic acid in two commercially available tablets and provided an excellent recovery (i.e. 99.43 and 93.80%). Finally the method was applied in Orange and Pineapple fruits and 68.11±0.012 and 45.46±0.023 mg ascorbic acid per 100 g of the sample was found in each fruits respectively.
VL  - 4
IS  - 1
ER  -

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