Anthelmintic and Antimicrobial Activities of Tannin Extracts of <i>Mitragyna inermis </i>(Willd.) Kuntze (Rubiaceae) and <i>Combretum glutinosum </i>Perr. ex DC (Combretaceae)

Eléonore Chikani Ladekan-Yayi; Placide Mahougnan Toklo; Durand Dah-Nouvlessounon; Mahoudo Fidèle Assogba; Steven Collins Njonté Wouamba; Billy Toussie Tchegnitegni; Géorcelin Goué Alowanou; Lamine Baba-Moussa; Sylvie Hounzangbe-Adote; Bruno Ndjakou Lenta; Siméon Fogue Kouam; Gbenou Joachim Djimon

doi:doi:10.11648/j.ajac.20210905.14

Anthelmintic and Antimicrobial Activities of Tannin Extracts of Mitragyna inermis (Willd.) Kuntze (Rubiaceae) and Combretum glutinosum Perr. ex DC (Combretaceae)

Eléonore Chikani Ladekan-Yayi, Placide Mahougnan Toklo, Durand Dah-Nouvlessounon, Mahoudo Fidèle Assogba, Steven Collins Njonté Wouamba, Billy Toussie Tchegnitegni, Géorcelin Goué Alowanou, Lamine Baba-Moussa, Sylvie Hounzangbe-Adote, Bruno Ndjakou Lenta, Siméon Fogue Kouam, Gbenou Joachim Djimon

Published in American Journal of Applied Chemistry (Volume 9, Issue 5)

Received: 30 August 2021 Accepted: 15 September 2021 Published: 26 September 2021

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Abstract

Combretum glutinosum and Mitragyna inermis are two plants used in Benin as a dewormer and antibiotic in traditional human and veterinary medicine. This study aimed to evaluate the anthelmintic, antimicrobial and antioxidant activities of the both plants tannin extracts. The extracts were tested in vitro on Haemonchus contortus larvae and worms and on the growth of 11 reference strains by agar medium diffusion method. Their chemical compositions were determined by UPLC-DAD-ESI-MS. It was found that total tannins extracted from plant leaves showed a strong inhibition on H. contortus larvae and adult worms compared to the negative control. Concerning the antimicrobial activity, M. inermis extract had an effect only on Pseudomonas aeruginosa with a MIC of 2.5 mg/mL. C. glutinosum extract inhibited the growth of most microbial strains with MIC values ranging from 1.25 to 20 mg/mL. The DPPH test showed that the extracts of C. glutinosum (IC₅₀ = 8.04 µg/mL) and M. inermis (IC₅₀ = 11.21 µg/mL) have good antioxidant activity and these results are confirmed by the FRAP method. Four (4) compounds could be identified in the tannin extract of C. glutinosum and could explain its activities. The results obtained from this work revealed that the tannins extracted from C. glutinosum showed better anthelmintic, antimicrobial and antioxidant activities compared to the extracts from M. inermis.

Published in	American Journal of Applied Chemistry (Volume 9, Issue 5)
DOI	10.11648/j.ajac.20210905.14
Page(s)	145-153
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2021. Published by Science Publishing Group

Keywords

M. inermis, C. glutinosum, Total Tannins, Anthelmintics, Antimicrobials, Antioxidants, LC-MS

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Eléonore Chikani Ladekan-Yayi, Placide Mahougnan Toklo, Durand Dah-Nouvlessounon, Mahoudo Fidèle Assogba, Steven Collins Njonté Wouamba, et al. (2021). Anthelmintic and Antimicrobial Activities of Tannin Extracts of Mitragyna inermis (Willd.) Kuntze (Rubiaceae) and Combretum glutinosum Perr. ex DC (Combretaceae). American Journal of Applied Chemistry, 9(5), 145-153. https://doi.org/10.11648/j.ajac.20210905.14

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Eléonore Chikani Ladekan-Yayi; Placide Mahougnan Toklo; Durand Dah-Nouvlessounon; Mahoudo Fidèle Assogba; Steven Collins Njonté Wouamba, et al. Anthelmintic and Antimicrobial Activities of Tannin Extracts of Mitragyna inermis (Willd.) Kuntze (Rubiaceae) and Combretum glutinosum Perr. ex DC (Combretaceae). Am. J. Appl. Chem. 2021, 9(5), 145-153. doi: 10.11648/j.ajac.20210905.14

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AMA Style

Eléonore Chikani Ladekan-Yayi, Placide Mahougnan Toklo, Durand Dah-Nouvlessounon, Mahoudo Fidèle Assogba, Steven Collins Njonté Wouamba, et al. Anthelmintic and Antimicrobial Activities of Tannin Extracts of Mitragyna inermis (Willd.) Kuntze (Rubiaceae) and Combretum glutinosum Perr. ex DC (Combretaceae). Am J Appl Chem. 2021;9(5):145-153. doi: 10.11648/j.ajac.20210905.14

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@article{10.11648/j.ajac.20210905.14,
  author = {Eléonore Chikani Ladekan-Yayi and Placide Mahougnan Toklo and Durand Dah-Nouvlessounon and Mahoudo Fidèle Assogba and Steven Collins Njonté Wouamba and Billy Toussie Tchegnitegni and Géorcelin Goué Alowanou and Lamine Baba-Moussa and Sylvie Hounzangbe-Adote and Bruno Ndjakou Lenta and Siméon Fogue Kouam and Gbenou Joachim Djimon},
  title = {Anthelmintic and Antimicrobial Activities of Tannin Extracts of Mitragyna inermis (Willd.) Kuntze (Rubiaceae) and Combretum glutinosum Perr. ex DC (Combretaceae)},
  journal = {American Journal of Applied Chemistry},
  volume = {9},
  number = {5},
  pages = {145-153},
  doi = {10.11648/j.ajac.20210905.14},
  url = {https://doi.org/10.11648/j.ajac.20210905.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajac.20210905.14},
  abstract = {Combretum glutinosum and Mitragyna inermis are two plants used in Benin as a dewormer and antibiotic in traditional human and veterinary medicine. This study aimed to evaluate the anthelmintic, antimicrobial and antioxidant activities of the both plants tannin extracts. The extracts were tested in vitro on Haemonchus contortus larvae and worms and on the growth of 11 reference strains by agar medium diffusion method. Their chemical compositions were determined by UPLC-DAD-ESI-MS. It was found that total tannins extracted from plant leaves showed a strong inhibition on H. contortus larvae and adult worms compared to the negative control. Concerning the antimicrobial activity, M. inermis extract had an effect only on Pseudomonas aeruginosa with a MIC of 2.5 mg/mL. C. glutinosum extract inhibited the growth of most microbial strains with MIC values ranging from 1.25 to 20 mg/mL. The DPPH test showed that the extracts of C. glutinosum (IC50 = 8.04 µg/mL) and M. inermis (IC50 = 11.21 µg/mL) have good antioxidant activity and these results are confirmed by the FRAP method. Four (4) compounds could be identified in the tannin extract of C. glutinosum and could explain its activities. The results obtained from this work revealed that the tannins extracted from C. glutinosum showed better anthelmintic, antimicrobial and antioxidant activities compared to the extracts from M. inermis.},
 year = {2021}
}

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TY - JOUR
T1 - Anthelmintic and Antimicrobial Activities of Tannin Extracts of Mitragyna inermis (Willd.) Kuntze (Rubiaceae) and Combretum glutinosum Perr. ex DC (Combretaceae)
AU - Eléonore Chikani Ladekan-Yayi
AU - Placide Mahougnan Toklo
AU - Durand Dah-Nouvlessounon
AU - Mahoudo Fidèle Assogba
AU - Steven Collins Njonté Wouamba
AU - Billy Toussie Tchegnitegni
AU - Géorcelin Goué Alowanou
AU - Lamine Baba-Moussa
AU - Sylvie Hounzangbe-Adote
AU - Bruno Ndjakou Lenta
AU - Siméon Fogue Kouam
AU - Gbenou Joachim Djimon
Y1 - 2021/09/26
PY - 2021
N1 - https://doi.org/10.11648/j.ajac.20210905.14
DO - 10.11648/j.ajac.20210905.14
T2 - American Journal of Applied Chemistry
JF - American Journal of Applied Chemistry
JO - American Journal of Applied Chemistry
SP - 145
EP - 153
PB - Science Publishing Group
SN - 2330-8745
UR - https://doi.org/10.11648/j.ajac.20210905.14
AB - Combretum glutinosum and Mitragyna inermis are two plants used in Benin as a dewormer and antibiotic in traditional human and veterinary medicine. This study aimed to evaluate the anthelmintic, antimicrobial and antioxidant activities of the both plants tannin extracts. The extracts were tested in vitro on Haemonchus contortus larvae and worms and on the growth of 11 reference strains by agar medium diffusion method. Their chemical compositions were determined by UPLC-DAD-ESI-MS. It was found that total tannins extracted from plant leaves showed a strong inhibition on H. contortus larvae and adult worms compared to the negative control. Concerning the antimicrobial activity, M. inermis extract had an effect only on Pseudomonas aeruginosa with a MIC of 2.5 mg/mL. C. glutinosum extract inhibited the growth of most microbial strains with MIC values ranging from 1.25 to 20 mg/mL. The DPPH test showed that the extracts of C. glutinosum (IC50 = 8.04 µg/mL) and M. inermis (IC50 = 11.21 µg/mL) have good antioxidant activity and these results are confirmed by the FRAP method. Four (4) compounds could be identified in the tannin extract of C. glutinosum and could explain its activities. The results obtained from this work revealed that the tannins extracted from C. glutinosum showed better anthelmintic, antimicrobial and antioxidant activities compared to the extracts from M. inermis.
VL - 9
IS - 5
ER -

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Author Information

Eléonore Chikani Ladekan-Yayi

Department of Chemistry, University of Abomey-Calavi, Abomey-Calavi, Benin
Placide Mahougnan Toklo

Department of Chemistry, University of Abomey-Calavi, Abomey-Calavi, Benin
Durand Dah-Nouvlessounon

Faculty of Sciences and Technics, University of Abomey-Calavi, Abomey-Calavi, Benin
Mahoudo Fidèle Assogba

Department of Chemistry, University of Abomey-Calavi, Abomey-Calavi, Benin
Steven Collins Njonté Wouamba

Department of Chemistry, University of Yaounde I, Yaounde, Cameroon
Billy Toussie Tchegnitegni

Department of Chemistry, University of Dschang, Dschang, Cameroon
Géorcelin Goué Alowanou

Department of Science and Technnology of Animal Production, University of Abomey Calavi, Abomey-Calavi, Benin
Lamine Baba-Moussa

Faculty of Sciences and Technics, University of Abomey-Calavi, Abomey-Calavi, Benin
Sylvie Hounzangbe-Adote

Department of Science and Technnology of Animal Production, University of Abomey Calavi, Abomey-Calavi, Benin
Bruno Ndjakou Lenta

Department of Chemistry, University of Yaounde I, Yaounde, Cameroon
Siméon Fogue Kouam

Department of Chemistry, University of Yaounde I, Yaounde, Cameroon
Gbenou Joachim Djimon

Department of Chemistry, National University of Science, Technology, Engineering and Mathematics, Abomey, Benin

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Eléonore Chikani Ladekan-Yayi, Placide Mahougnan Toklo, Durand Dah-Nouvlessounon, Mahoudo Fidèle Assogba, Steven Collins Njonté Wouamba, et al. (2021). Anthelmintic and Antimicrobial Activities of Tannin Extracts of Mitragyna inermis (Willd.) Kuntze (Rubiaceae) and Combretum glutinosum Perr. ex DC (Combretaceae). American Journal of Applied Chemistry, 9(5), 145-153. https://doi.org/10.11648/j.ajac.20210905.14

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ACS Style

Eléonore Chikani Ladekan-Yayi; Placide Mahougnan Toklo; Durand Dah-Nouvlessounon; Mahoudo Fidèle Assogba; Steven Collins Njonté Wouamba, et al. Anthelmintic and Antimicrobial Activities of Tannin Extracts of Mitragyna inermis (Willd.) Kuntze (Rubiaceae) and Combretum glutinosum Perr. ex DC (Combretaceae). Am. J. Appl. Chem. 2021, 9(5), 145-153. doi: 10.11648/j.ajac.20210905.14

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AMA Style

Eléonore Chikani Ladekan-Yayi, Placide Mahougnan Toklo, Durand Dah-Nouvlessounon, Mahoudo Fidèle Assogba, Steven Collins Njonté Wouamba, et al. Anthelmintic and Antimicrobial Activities of Tannin Extracts of Mitragyna inermis (Willd.) Kuntze (Rubiaceae) and Combretum glutinosum Perr. ex DC (Combretaceae). Am J Appl Chem. 2021;9(5):145-153. doi: 10.11648/j.ajac.20210905.14

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@article{10.11648/j.ajac.20210905.14,
  author = {Eléonore Chikani Ladekan-Yayi and Placide Mahougnan Toklo and Durand Dah-Nouvlessounon and Mahoudo Fidèle Assogba and Steven Collins Njonté Wouamba and Billy Toussie Tchegnitegni and Géorcelin Goué Alowanou and Lamine Baba-Moussa and Sylvie Hounzangbe-Adote and Bruno Ndjakou Lenta and Siméon Fogue Kouam and Gbenou Joachim Djimon},
  title = {Anthelmintic and Antimicrobial Activities of Tannin Extracts of Mitragyna inermis (Willd.) Kuntze (Rubiaceae) and Combretum glutinosum Perr. ex DC (Combretaceae)},
  journal = {American Journal of Applied Chemistry},
  volume = {9},
  number = {5},
  pages = {145-153},
  doi = {10.11648/j.ajac.20210905.14},
  url = {https://doi.org/10.11648/j.ajac.20210905.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajac.20210905.14},
  abstract = {Combretum glutinosum and Mitragyna inermis are two plants used in Benin as a dewormer and antibiotic in traditional human and veterinary medicine. This study aimed to evaluate the anthelmintic, antimicrobial and antioxidant activities of the both plants tannin extracts. The extracts were tested in vitro on Haemonchus contortus larvae and worms and on the growth of 11 reference strains by agar medium diffusion method. Their chemical compositions were determined by UPLC-DAD-ESI-MS. It was found that total tannins extracted from plant leaves showed a strong inhibition on H. contortus larvae and adult worms compared to the negative control. Concerning the antimicrobial activity, M. inermis extract had an effect only on Pseudomonas aeruginosa with a MIC of 2.5 mg/mL. C. glutinosum extract inhibited the growth of most microbial strains with MIC values ranging from 1.25 to 20 mg/mL. The DPPH test showed that the extracts of C. glutinosum (IC50 = 8.04 µg/mL) and M. inermis (IC50 = 11.21 µg/mL) have good antioxidant activity and these results are confirmed by the FRAP method. Four (4) compounds could be identified in the tannin extract of C. glutinosum and could explain its activities. The results obtained from this work revealed that the tannins extracted from C. glutinosum showed better anthelmintic, antimicrobial and antioxidant activities compared to the extracts from M. inermis.},
 year = {2021}
}

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