Vernonia Amygdalina is a well-known and widely used plant in traditional African medicine. The decoctions of its leaves are used in the treatment of many pathologies including hepatitis, diabetes, cancer etc. It is a plant very rich in phenolic compounds, generally responsible for the biological activity of plants. In this study, we determined the optimal extraction conditions, including temperature, duration, and solvent mass/volume ratio. Subsequently, the total polyphenol contents are determined under these optimal conditions, then the antiradical activity evaluated. The Folin-Ciocalteu reagent was used to evaluate the content of phenolic compounds, with gallic acid as a reference. The radical DPPH· made it possible to measure the antioxidant power of the extract, with reference to Trolox. This study showed that the optimal conditions for extracting the bioactive molecules responsible for the antioxidant activity are obtained for a temperature of 80°C, for a period of 30 min in a ratio of 1%. Under these conditions, the total polyphenol and flavonoid contents are respectively equal to 10.536 ± 0.145 mg/g gallic acid equivalent and 1.930 ± 0.043 mg/g quercetin equivalent. Furthermore, the results show a maximum inhibition of 41.95% for a concentration of 0.044 mg/mL, for 30 minutes. The antioxidant properties were measured and demonstrated by the kinetics of reduction with DPPH·, thus showing an inhibitory power of 75% and a fairly slow reduction reaction. This high content of phenolic compounds and the fairly significant inhibitory power of Vernonia amygdalina justify the use of this plant in traditional medicine for the treatment of numerous pathologies.
| Published in | American Journal of Applied Chemistry (Volume 10, Issue 6) |
| DOI | 10.11648/j.ajac.20221006.12 |
| Page(s) | 176-182 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2022. Published by Science Publishing Group |
Vernonia amygdalina, Extraction, Polyphenols, Flavonoids, Antioxidant Activity
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APA Style
Bedie Mbow, Aïssatou Alioune Gaye, Birane Diop, Abba Diarra Kone, Ibrahima Diallo, et al. (2022). Optimization of Extraction Parameters, Total Polyphenols and Flavonoids Contents, and Antioxidant Activity of the Aqueous Extract of Vernonia amygdalina Leaves. American Journal of Applied Chemistry, 10(6), 176-182. https://doi.org/10.11648/j.ajac.20221006.12
ACS Style
Bedie Mbow; Aïssatou Alioune Gaye; Birane Diop; Abba Diarra Kone; Ibrahima Diallo, et al. Optimization of Extraction Parameters, Total Polyphenols and Flavonoids Contents, and Antioxidant Activity of the Aqueous Extract of Vernonia amygdalina Leaves. Am. J. Appl. Chem. 2022, 10(6), 176-182. doi: 10.11648/j.ajac.20221006.12
AMA Style
Bedie Mbow, Aïssatou Alioune Gaye, Birane Diop, Abba Diarra Kone, Ibrahima Diallo, et al. Optimization of Extraction Parameters, Total Polyphenols and Flavonoids Contents, and Antioxidant Activity of the Aqueous Extract of Vernonia amygdalina Leaves. Am J Appl Chem. 2022;10(6):176-182. doi: 10.11648/j.ajac.20221006.12
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Download@article{10.11648/j.ajac.20221006.12,
author = {Bedie Mbow and Aïssatou Alioune Gaye and Birane Diop and Abba Diarra Kone and Ibrahima Diallo and Mohamed Gaye},
title = {Optimization of Extraction Parameters, Total Polyphenols and Flavonoids Contents, and Antioxidant Activity of the Aqueous Extract of Vernonia amygdalina Leaves},
journal = {American Journal of Applied Chemistry},
volume = {10},
number = {6},
pages = {176-182},
doi = {10.11648/j.ajac.20221006.12},
url = {https://doi.org/10.11648/j.ajac.20221006.12},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajac.20221006.12},
abstract = {Vernonia Amygdalina is a well-known and widely used plant in traditional African medicine. The decoctions of its leaves are used in the treatment of many pathologies including hepatitis, diabetes, cancer etc. It is a plant very rich in phenolic compounds, generally responsible for the biological activity of plants. In this study, we determined the optimal extraction conditions, including temperature, duration, and solvent mass/volume ratio. Subsequently, the total polyphenol contents are determined under these optimal conditions, then the antiradical activity evaluated. The Folin-Ciocalteu reagent was used to evaluate the content of phenolic compounds, with gallic acid as a reference. The radical DPPH· made it possible to measure the antioxidant power of the extract, with reference to Trolox. This study showed that the optimal conditions for extracting the bioactive molecules responsible for the antioxidant activity are obtained for a temperature of 80°C, for a period of 30 min in a ratio of 1%. Under these conditions, the total polyphenol and flavonoid contents are respectively equal to 10.536 ± 0.145 mg/g gallic acid equivalent and 1.930 ± 0.043 mg/g quercetin equivalent. Furthermore, the results show a maximum inhibition of 41.95% for a concentration of 0.044 mg/mL, for 30 minutes. The antioxidant properties were measured and demonstrated by the kinetics of reduction with DPPH·, thus showing an inhibitory power of 75% and a fairly slow reduction reaction. This high content of phenolic compounds and the fairly significant inhibitory power of Vernonia amygdalina justify the use of this plant in traditional medicine for the treatment of numerous pathologies.},
year = {2022}
}
TY - JOUR T1 - Optimization of Extraction Parameters, Total Polyphenols and Flavonoids Contents, and Antioxidant Activity of the Aqueous Extract of Vernonia amygdalina Leaves AU - Bedie Mbow AU - Aïssatou Alioune Gaye AU - Birane Diop AU - Abba Diarra Kone AU - Ibrahima Diallo AU - Mohamed Gaye Y1 - 2022/11/30 PY - 2022 N1 - https://doi.org/10.11648/j.ajac.20221006.12 DO - 10.11648/j.ajac.20221006.12 T2 - American Journal of Applied Chemistry JF - American Journal of Applied Chemistry JO - American Journal of Applied Chemistry SP - 176 EP - 182 PB - Science Publishing Group SN - 2330-8745 UR - https://doi.org/10.11648/j.ajac.20221006.12 AB - Vernonia Amygdalina is a well-known and widely used plant in traditional African medicine. The decoctions of its leaves are used in the treatment of many pathologies including hepatitis, diabetes, cancer etc. It is a plant very rich in phenolic compounds, generally responsible for the biological activity of plants. In this study, we determined the optimal extraction conditions, including temperature, duration, and solvent mass/volume ratio. Subsequently, the total polyphenol contents are determined under these optimal conditions, then the antiradical activity evaluated. The Folin-Ciocalteu reagent was used to evaluate the content of phenolic compounds, with gallic acid as a reference. The radical DPPH· made it possible to measure the antioxidant power of the extract, with reference to Trolox. This study showed that the optimal conditions for extracting the bioactive molecules responsible for the antioxidant activity are obtained for a temperature of 80°C, for a period of 30 min in a ratio of 1%. Under these conditions, the total polyphenol and flavonoid contents are respectively equal to 10.536 ± 0.145 mg/g gallic acid equivalent and 1.930 ± 0.043 mg/g quercetin equivalent. Furthermore, the results show a maximum inhibition of 41.95% for a concentration of 0.044 mg/mL, for 30 minutes. The antioxidant properties were measured and demonstrated by the kinetics of reduction with DPPH·, thus showing an inhibitory power of 75% and a fairly slow reduction reaction. This high content of phenolic compounds and the fairly significant inhibitory power of Vernonia amygdalina justify the use of this plant in traditional medicine for the treatment of numerous pathologies. VL - 10 IS - 6 ER -
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