Preparation of Nano-hydroxyapatite Obtained from Lates Calcarifer Fish Bone by Alkaline Hydrolysis Method

Le Ho Khanh Hy; Dao Viet Ha; Pham Xuan Ky; Nguyen Phuong Anh; Phan Bao Vy; Doan Thi Thiet

doi:doi:10.11648/j.ajcbe.20210501.15

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Preparation of Nano-hydroxyapatite Obtained from Lates Calcarifer Fish Bone by Alkaline Hydrolysis Method

Le Ho Khanh Hy, Dao Viet Ha, Pham Xuan Ky, Nguyen Phuong Anh, Phan Bao Vy, Doan Thi Thiet

Published in American Journal of Chemical and Biochemical Engineering (Volume 5, Issue 1)

Received: 20 May 2021 Accepted: 11 June 2021 Published: 22 June 2021

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Abstract

Fish bone by-products are considered as abundant and cheap sources of Hydroxyapatite (HAp). The preparation of HAp powders from fish bones not only contributes to improving the added value of by-products but also reduces undesirable impacts on the environment. In this study, nano-HAp was successfully obtained from Lates calcarifer fish bone originated from a seafood export company in Khanh Hoa province. After pretreatment of fish bones for removing organic matters, the bones were under alkaline hydrolysis at 200°C within different time intervals of 30 mins, 1 and 1.5 hours. Results of XRD and SEM analysis showed that the calcium formed was HAP and it possessed an average size of 50-64 nm. The values of the Ca/P molar ratio from 1.896 to 1.921 prove that the nano-HAp powders are B-type biological hydroxyapatites which have been confirmed by FTIR spectrum. In addition, the contents of heavy metals such as As, Pb, Hg, Cd are measured by emission spectrophotometer and detected within safety limits of regulatory requirements of Vietnam regulation and US Pharmacopeia for food and dietary supplement standard. These properties show that nano-HAp from Lates calcarifer fish bone are applicable and to be used as an input material in food and medicine field.

Published in	American Journal of Chemical and Biochemical Engineering (Volume 5, Issue 1)
DOI	10.11648/j.ajcbe.20210501.15
Page(s)	32-40
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2021. Published by Science Publishing Group

Keywords

Lates Calcarifer Fish Bone, Alkakine Hydrolysis, 200°C, 0.5/1/1.5 Hours, Nanohydroxyapatite, B-type Biological Hydroxyapatite

References

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APA Style

Le Ho Khanh Hy, Dao Viet Ha, Pham Xuan Ky, Nguyen Phuong Anh, Phan Bao Vy, et al. (2021). Preparation of Nano-hydroxyapatite Obtained from Lates Calcarifer Fish Bone by Alkaline Hydrolysis Method. American Journal of Chemical and Biochemical Engineering, 5(1), 32-40. https://doi.org/10.11648/j.ajcbe.20210501.15

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ACS Style

Le Ho Khanh Hy; Dao Viet Ha; Pham Xuan Ky; Nguyen Phuong Anh; Phan Bao Vy, et al. Preparation of Nano-hydroxyapatite Obtained from Lates Calcarifer Fish Bone by Alkaline Hydrolysis Method. Am. J. Chem. Biochem. Eng. 2021, 5(1), 32-40. doi: 10.11648/j.ajcbe.20210501.15

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AMA Style

Le Ho Khanh Hy, Dao Viet Ha, Pham Xuan Ky, Nguyen Phuong Anh, Phan Bao Vy, et al. Preparation of Nano-hydroxyapatite Obtained from Lates Calcarifer Fish Bone by Alkaline Hydrolysis Method. Am J Chem Biochem Eng. 2021;5(1):32-40. doi: 10.11648/j.ajcbe.20210501.15

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@article{10.11648/j.ajcbe.20210501.15,
  author = {Le Ho Khanh Hy and Dao Viet Ha and Pham Xuan Ky and Nguyen Phuong Anh and Phan Bao Vy and Doan Thi Thiet},
  title = {Preparation of Nano-hydroxyapatite Obtained from Lates Calcarifer Fish Bone by Alkaline Hydrolysis Method},
  journal = {American Journal of Chemical and Biochemical Engineering},
  volume = {5},
  number = {1},
  pages = {32-40},
  doi = {10.11648/j.ajcbe.20210501.15},
  url = {https://doi.org/10.11648/j.ajcbe.20210501.15},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajcbe.20210501.15},
  abstract = {Fish bone by-products are considered as abundant and cheap sources of Hydroxyapatite (HAp). The preparation of HAp powders from fish bones not only contributes to improving the added value of by-products but also reduces undesirable impacts on the environment. In this study, nano-HAp was successfully obtained from Lates calcarifer fish bone originated from a seafood export company in Khanh Hoa province. After pretreatment of fish bones for removing organic matters, the bones were under alkaline hydrolysis at 200°C within different time intervals of 30 mins, 1 and 1.5 hours. Results of XRD and SEM analysis showed that the calcium formed was HAP and it possessed an average size of 50-64 nm. The values of the Ca/P molar ratio from 1.896 to 1.921 prove that the nano-HAp powders are B-type biological hydroxyapatites which have been confirmed by FTIR spectrum. In addition, the contents of heavy metals such as As, Pb, Hg, Cd are measured by emission spectrophotometer and detected within safety limits of regulatory requirements of Vietnam regulation and US Pharmacopeia for food and dietary supplement standard. These properties show that nano-HAp from Lates calcarifer fish bone are applicable and to be used as an input material in food and medicine field.},
 year = {2021}
}

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TY  - JOUR
T1  - Preparation of Nano-hydroxyapatite Obtained from Lates Calcarifer Fish Bone by Alkaline Hydrolysis Method
AU  - Le Ho Khanh Hy
AU  - Dao Viet Ha
AU  - Pham Xuan Ky
AU  - Nguyen Phuong Anh
AU  - Phan Bao Vy
AU  - Doan Thi Thiet
Y1  - 2021/06/22
PY  - 2021
N1  - https://doi.org/10.11648/j.ajcbe.20210501.15
DO  - 10.11648/j.ajcbe.20210501.15
T2  - American Journal of Chemical and Biochemical Engineering
JF  - American Journal of Chemical and Biochemical Engineering
JO  - American Journal of Chemical and Biochemical Engineering
SP  - 32
EP  - 40
PB  - Science Publishing Group
SN  - 2639-9989
UR  - https://doi.org/10.11648/j.ajcbe.20210501.15
AB  - Fish bone by-products are considered as abundant and cheap sources of Hydroxyapatite (HAp). The preparation of HAp powders from fish bones not only contributes to improving the added value of by-products but also reduces undesirable impacts on the environment. In this study, nano-HAp was successfully obtained from Lates calcarifer fish bone originated from a seafood export company in Khanh Hoa province. After pretreatment of fish bones for removing organic matters, the bones were under alkaline hydrolysis at 200°C within different time intervals of 30 mins, 1 and 1.5 hours. Results of XRD and SEM analysis showed that the calcium formed was HAP and it possessed an average size of 50-64 nm. The values of the Ca/P molar ratio from 1.896 to 1.921 prove that the nano-HAp powders are B-type biological hydroxyapatites which have been confirmed by FTIR spectrum. In addition, the contents of heavy metals such as As, Pb, Hg, Cd are measured by emission spectrophotometer and detected within safety limits of regulatory requirements of Vietnam regulation and US Pharmacopeia for food and dietary supplement standard. These properties show that nano-HAp from Lates calcarifer fish bone are applicable and to be used as an input material in food and medicine field.
VL  - 5
IS  - 1
ER  -

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Author Information

Le Ho Khanh Hy

Institute of Oceanography, Vietnam Academy of Science and Technology (VAST), Nha Trang, Khanh Hoa, Vietnam
Dao Viet Ha

Institute of Oceanography, Vietnam Academy of Science and Technology (VAST), Nha Trang, Khanh Hoa, Vietnam
Pham Xuan Ky

Institute of Oceanography, Vietnam Academy of Science and Technology (VAST), Nha Trang, Khanh Hoa, Vietnam
Nguyen Phuong Anh

Institute of Oceanography, Vietnam Academy of Science and Technology (VAST), Nha Trang, Khanh Hoa, Vietnam
Phan Bao Vy

Institute of Oceanography, Vietnam Academy of Science and Technology (VAST), Nha Trang, Khanh Hoa, Vietnam
Doan Thi Thiet

Institute of Oceanography, Vietnam Academy of Science and Technology (VAST), Nha Trang, Khanh Hoa, Vietnam

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Le Ho Khanh Hy, Dao Viet Ha, Pham Xuan Ky, Nguyen Phuong Anh, Phan Bao Vy, et al. (2021). Preparation of Nano-hydroxyapatite Obtained from Lates Calcarifer Fish Bone by Alkaline Hydrolysis Method. American Journal of Chemical and Biochemical Engineering, 5(1), 32-40. https://doi.org/10.11648/j.ajcbe.20210501.15

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ACS Style

Le Ho Khanh Hy; Dao Viet Ha; Pham Xuan Ky; Nguyen Phuong Anh; Phan Bao Vy, et al. Preparation of Nano-hydroxyapatite Obtained from Lates Calcarifer Fish Bone by Alkaline Hydrolysis Method. Am. J. Chem. Biochem. Eng. 2021, 5(1), 32-40. doi: 10.11648/j.ajcbe.20210501.15

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AMA Style

Le Ho Khanh Hy, Dao Viet Ha, Pham Xuan Ky, Nguyen Phuong Anh, Phan Bao Vy, et al. Preparation of Nano-hydroxyapatite Obtained from Lates Calcarifer Fish Bone by Alkaline Hydrolysis Method. Am J Chem Biochem Eng. 2021;5(1):32-40. doi: 10.11648/j.ajcbe.20210501.15

Copy | Download

@article{10.11648/j.ajcbe.20210501.15,
  author = {Le Ho Khanh Hy and Dao Viet Ha and Pham Xuan Ky and Nguyen Phuong Anh and Phan Bao Vy and Doan Thi Thiet},
  title = {Preparation of Nano-hydroxyapatite Obtained from Lates Calcarifer Fish Bone by Alkaline Hydrolysis Method},
  journal = {American Journal of Chemical and Biochemical Engineering},
  volume = {5},
  number = {1},
  pages = {32-40},
  doi = {10.11648/j.ajcbe.20210501.15},
  url = {https://doi.org/10.11648/j.ajcbe.20210501.15},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajcbe.20210501.15},
  abstract = {Fish bone by-products are considered as abundant and cheap sources of Hydroxyapatite (HAp). The preparation of HAp powders from fish bones not only contributes to improving the added value of by-products but also reduces undesirable impacts on the environment. In this study, nano-HAp was successfully obtained from Lates calcarifer fish bone originated from a seafood export company in Khanh Hoa province. After pretreatment of fish bones for removing organic matters, the bones were under alkaline hydrolysis at 200°C within different time intervals of 30 mins, 1 and 1.5 hours. Results of XRD and SEM analysis showed that the calcium formed was HAP and it possessed an average size of 50-64 nm. The values of the Ca/P molar ratio from 1.896 to 1.921 prove that the nano-HAp powders are B-type biological hydroxyapatites which have been confirmed by FTIR spectrum. In addition, the contents of heavy metals such as As, Pb, Hg, Cd are measured by emission spectrophotometer and detected within safety limits of regulatory requirements of Vietnam regulation and US Pharmacopeia for food and dietary supplement standard. These properties show that nano-HAp from Lates calcarifer fish bone are applicable and to be used as an input material in food and medicine field.},
 year = {2021}
}

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TY  - JOUR
T1  - Preparation of Nano-hydroxyapatite Obtained from Lates Calcarifer Fish Bone by Alkaline Hydrolysis Method
AU  - Le Ho Khanh Hy
AU  - Dao Viet Ha
AU  - Pham Xuan Ky
AU  - Nguyen Phuong Anh
AU  - Phan Bao Vy
AU  - Doan Thi Thiet
Y1  - 2021/06/22
PY  - 2021
N1  - https://doi.org/10.11648/j.ajcbe.20210501.15
DO  - 10.11648/j.ajcbe.20210501.15
T2  - American Journal of Chemical and Biochemical Engineering
JF  - American Journal of Chemical and Biochemical Engineering
JO  - American Journal of Chemical and Biochemical Engineering
SP  - 32
EP  - 40
PB  - Science Publishing Group
SN  - 2639-9989
UR  - https://doi.org/10.11648/j.ajcbe.20210501.15
AB  - Fish bone by-products are considered as abundant and cheap sources of Hydroxyapatite (HAp). The preparation of HAp powders from fish bones not only contributes to improving the added value of by-products but also reduces undesirable impacts on the environment. In this study, nano-HAp was successfully obtained from Lates calcarifer fish bone originated from a seafood export company in Khanh Hoa province. After pretreatment of fish bones for removing organic matters, the bones were under alkaline hydrolysis at 200°C within different time intervals of 30 mins, 1 and 1.5 hours. Results of XRD and SEM analysis showed that the calcium formed was HAP and it possessed an average size of 50-64 nm. The values of the Ca/P molar ratio from 1.896 to 1.921 prove that the nano-HAp powders are B-type biological hydroxyapatites which have been confirmed by FTIR spectrum. In addition, the contents of heavy metals such as As, Pb, Hg, Cd are measured by emission spectrophotometer and detected within safety limits of regulatory requirements of Vietnam regulation and US Pharmacopeia for food and dietary supplement standard. These properties show that nano-HAp from Lates calcarifer fish bone are applicable and to be used as an input material in food and medicine field.
VL  - 5
IS  - 1
ER  -

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