Objective: To investigate the neuroprotective effects of grape seed proanthocyanidin extract（GSPE）on a model of neonatal hypoxic ischemic encephalopathy (HIE), we investigated the changes in neuronal cells and astrocytes after pre-treatment with GSPE. Methods: Seven-day-old pups were randomly divided into sham, HI, and GSPE+HI groups. The HIE model was established using a modified Rice-Vannucci method. GSPE was injected intraperitoneally 20 min before surgery. The change in markers of neuronal cells and astrocytes (NeuN/GFAP) were detected by immunofluorescence and Western blot. Results: Compared with the sham group, the expression of NeuN in the HI group was significantly reduced, and the expression of NeuN was significantly increased after GSPE pre-treatment. The expression of GFAP was opposite to NeuN. Conclusion: Our study showed that GSPE pre-treatment significantly protected neurons and inhibited astrocyte over-proliferation. Therefore, we believe that GSPE is a potential drug for the treatment of HIE and can prevent brain damage caused by hypoxia and ischemia.
| Published in | American Journal of Pediatrics (Volume 5, Issue 2) |
| DOI | 10.11648/j.ajp.20190502.13 |
| Page(s) | 51-55 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2019. Published by Science Publishing Group |
Grape Seed Proanthocyanidin Extract, NeuN, GFAP, Neonatal Hypoxic-Ischemic Brain Injury
| [1] | Liu G, Li Z G, Gao J S. Hypothermia in neonatal hypoxic-ischemic encephalopathy (HIE) [J]. Euro Review Medi Pharmacological Sci, 2017, 21: 50-53. |
| [2] | Rao R, Trivedi S, Distler A, et al. Neurodevelopmental Outcomes in Neonates with Mild Hypoxic Ischemic Encephalopathy Treated with Therapeutic Hypothermia [J]. Am J Perinatol, 2019. |
| [3] | Zhou X M, Liu J, Wang Y, et al. Silencing of long noncoding RNA MEG3 enhances cerebral protection of dexmedetomidine against hypoxic-ischemic brain damage in neonatal mice by binding to miR-129-5p [J]. J Cell Biochem, 2018. |
| [4] | Xu B, Xiao A J, Chen W, et al. Neuroprotective effects of a PSD-95 inhibitor in neonatal hypoxic-ischemic brain injury [J]. Molecular Neurobi, 2016, 53: 5962-5970. |
| [5] | Luo Z, Zhang M, Niu X, et al. Inhibition of the PI3K/Akt signaling pathway impedes the restoration of neurological function following hypoxic-ischemic brain damage in a neonatal rabbit model [J]. J Cell Biochem, 2019. |
| [6] | Devi A, Jolitha A B, Ishii N. Grape Seed Proanthocyanidin Extract (GSPE) and antioxidant defense in the brain of adult rats [J]. Medi Sci Mo, 2006, 12: BR124-BR129. |
| [7] | Liu B, Zhang H, Tan X, et al. GSPE reduces lead-induced oxidative stress by activating the Nrf2 pathway and suppressing miR153 and GSK-3β in rat kidney [J]. Oncotarget, 2017, 8: 42226-42237. |
| [8] | Rajput S, Sun L, Zhang N Y, et al. Grape Seed Proanthocyanidin Extract Alleviates AflatoxinB1-Induced Immunotoxicity and Oxidative Stress via Modulation of NF-κB and Nrf2 Signaling Pathways in Broilers [J]. Toxins, 2019, 11: 23. |
| [9] | Guo F, Hu Y, Niu Q, et al. Grape Seed Proanthocyanidin Extract Inhibits Human Esophageal Squamous Cancerous Cell Line ECA109 via the NF-κB Signaling Pathway [J]. Mediators Inflamm, 2018, 22: 6999-7012. |
| [10] | Hao J P, Shi H, Zhang J, et al. Role of GSPE in improving early cerebral vascular damage by inhibition of Profilin-1 expression in a ouabain-induced hypertension model [J]. Euro Review Medi Pharmacol Sci, 2018, 22: 6999-7012. |
| [11] | Sanna R S, Muthangi S, BK C S, et al. Grape seed proanthocyanidin extract and insulin prevents cognitive decline in type 1 diabetic rat by impacting Bcl-2 and Bax in the prefrontal cortex [J]. Metab Brain Dis, 2018: 1-15. |
| [12] | Fang M, Jiang H, Ye L, et al. Metformin treatment after the hypoxia-ischemia attenuates brain injury in newborn rats [J]. Oncotarget, 2017, 8: 75308-75325. |
| [13] | Al Mamun A, Yu H, Romana S, et al. Inflammatory Responses are Sex Specific in Chronic Hypoxic–Ischemic Encephalopathy [J]. Cell Trans, 2018. |
| [14] | Ye L, Feng Z, Doycheva D, et al. CpG-ODN exerts a neuroprotective effect via the TLR9/pAMPK signaling pathway by activation of autophagy in a neonatal HIE rat model [J]. Experi Neuro, 2018, 301: 70-80. |
| [15] | Jiang W, Guo M, Gong M, et al. Vitamin A bio-modulates apoptosis via the mitochondrial pathway after hypoxic-ischemic brain damage [J]. Mole Brain, 2018, 11: 14. |
| [16] | Birla H, Rai S N, Singh S S, et al. Tinospora cordifolia Suppresses Neuroinflammation in Parkinsonian Mouse Model [J]. NeuroMole Medi, 2019: 1-12. |
| [17] | Teismann P, Schulz J B. Cellular pathology of Parkinson’s disease: astrocytes, microglia and inflammation [J]. Cell Tissue Res, 2004, 318: 149-161. |
APA Style
Li Luo, Xing Tu, Xuexia Zhang, Jing Liu, Yilin Liu, et al. (2019). Grape Seed Proanthocyanidin Extract Adjusts NeuN/GFAP in a Murine Model of Neonatal Hypoxic-ischemic Brain Injury. American Journal of Pediatrics, 5(2), 51-55. https://doi.org/10.11648/j.ajp.20190502.13
ACS Style
Li Luo; Xing Tu; Xuexia Zhang; Jing Liu; Yilin Liu, et al. Grape Seed Proanthocyanidin Extract Adjusts NeuN/GFAP in a Murine Model of Neonatal Hypoxic-ischemic Brain Injury. Am. J. Pediatr. 2019, 5(2), 51-55. doi: 10.11648/j.ajp.20190502.13
AMA Style
Li Luo, Xing Tu, Xuexia Zhang, Jing Liu, Yilin Liu, et al. Grape Seed Proanthocyanidin Extract Adjusts NeuN/GFAP in a Murine Model of Neonatal Hypoxic-ischemic Brain Injury. Am J Pediatr. 2019;5(2):51-55. doi: 10.11648/j.ajp.20190502.13
Share This Article
Twitter
Linked In
Facebook
Copy
Download@article{10.11648/j.ajp.20190502.13,
author = {Li Luo and Xing Tu and Xuexia Zhang and Jing Liu and Yilin Liu and Wenyan Zhao and Ke Niu and Junhua Yang and Guoying Li and Mengxia Wang},
title = {Grape Seed Proanthocyanidin Extract Adjusts NeuN/GFAP in a Murine Model of Neonatal Hypoxic-ischemic Brain Injury},
journal = {American Journal of Pediatrics},
volume = {5},
number = {2},
pages = {51-55},
doi = {10.11648/j.ajp.20190502.13},
url = {https://doi.org/10.11648/j.ajp.20190502.13},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajp.20190502.13},
abstract = {Objective: To investigate the neuroprotective effects of grape seed proanthocyanidin extract（GSPE）on a model of neonatal hypoxic ischemic encephalopathy (HIE), we investigated the changes in neuronal cells and astrocytes after pre-treatment with GSPE. Methods: Seven-day-old pups were randomly divided into sham, HI, and GSPE+HI groups. The HIE model was established using a modified Rice-Vannucci method. GSPE was injected intraperitoneally 20 min before surgery. The change in markers of neuronal cells and astrocytes (NeuN/GFAP) were detected by immunofluorescence and Western blot. Results: Compared with the sham group, the expression of NeuN in the HI group was significantly reduced, and the expression of NeuN was significantly increased after GSPE pre-treatment. The expression of GFAP was opposite to NeuN. Conclusion: Our study showed that GSPE pre-treatment significantly protected neurons and inhibited astrocyte over-proliferation. Therefore, we believe that GSPE is a potential drug for the treatment of HIE and can prevent brain damage caused by hypoxia and ischemia.},
year = {2019}
}
TY - JOUR T1 - Grape Seed Proanthocyanidin Extract Adjusts NeuN/GFAP in a Murine Model of Neonatal Hypoxic-ischemic Brain Injury AU - Li Luo AU - Xing Tu AU - Xuexia Zhang AU - Jing Liu AU - Yilin Liu AU - Wenyan Zhao AU - Ke Niu AU - Junhua Yang AU - Guoying Li AU - Mengxia Wang Y1 - 2019/05/10 PY - 2019 N1 - https://doi.org/10.11648/j.ajp.20190502.13 DO - 10.11648/j.ajp.20190502.13 T2 - American Journal of Pediatrics JF - American Journal of Pediatrics JO - American Journal of Pediatrics SP - 51 EP - 55 PB - Science Publishing Group SN - 2472-0909 UR - https://doi.org/10.11648/j.ajp.20190502.13 AB - Objective: To investigate the neuroprotective effects of grape seed proanthocyanidin extract（GSPE）on a model of neonatal hypoxic ischemic encephalopathy (HIE), we investigated the changes in neuronal cells and astrocytes after pre-treatment with GSPE. Methods: Seven-day-old pups were randomly divided into sham, HI, and GSPE+HI groups. The HIE model was established using a modified Rice-Vannucci method. GSPE was injected intraperitoneally 20 min before surgery. The change in markers of neuronal cells and astrocytes (NeuN/GFAP) were detected by immunofluorescence and Western blot. Results: Compared with the sham group, the expression of NeuN in the HI group was significantly reduced, and the expression of NeuN was significantly increased after GSPE pre-treatment. The expression of GFAP was opposite to NeuN. Conclusion: Our study showed that GSPE pre-treatment significantly protected neurons and inhibited astrocyte over-proliferation. Therefore, we believe that GSPE is a potential drug for the treatment of HIE and can prevent brain damage caused by hypoxia and ischemia. VL - 5 IS - 2 ER -
Information
Email: