Introduction: Hirschsprung's disease is a congenital anomaly characterised by a rarefaction or absence of ganglion cells associated with schwannian hyperplasia or hypertrophy involving the colon or a segment of the colon. Its diagnosis is based on clinical, radiological and histological evidence. Haematoxylin-eosin examination is used in cases of suspected Hirschsprung's disease. However, in some situations it is insufficient to confirm or refute the diagnosis. Immunohistochemical and histo-enzymatic techniques can be used to demonstrate aganglionosis. The aim of our study is to evaluate the interest of calretinin in the diagnosis of Hirschsprung's disease in Dakar. Materials and methods: This was a retrospective and descriptive study from 1 January 2018 to 31 August 2019. It was conducted at the pathology laboratory of Idrissa Pouye General Hospital where 51 paraffin blocks from suspected Hirschsprung's Disease cases were included. All blocks were immunohistochemically studied with anti-calretinin antibody. The diagnosis of Hirschsprung's disease on standard histological examination was based on the absence of lymph node cells in the submucosal and myenteric plexuses. Calretinin immunoreactivity was shown by nuclear and cytoplasmic labelling of ganglion cells and nerve nets. In Hirschsprung's disease, there is an absence of labelling of the nerve plexuses. Results: The majority of patients (73.5%) were aged 2 years or older with a mean age of 3.4 years. The sex ratio was 2. Biopsies constituted 56.86% of the specimens and surgical specimens 43.14%. Concordance between haematoxylin-eosin examination and calretinin immunohistochemistry was observed in 47 cases (92.15%) and discordance in 4 cases (7.15%). The sensitivity of calretinin was 93.75% and the specificity 89.47%. The kappa index was 0.92. The recto-sigmoid form was the most frequent topographic form observed in 83.3% of patients. Conclusion: Morphological examination with haematoxylin-eosin remains a good diagnostic method for Hirschsprung's disease. Calretinin immunohistochemistry is necessary in equivocal cases, in neonates and infants and in case of superficial biopsies.
| Published in | American Journal of Pediatrics (Volume 7, Issue 3) |
| DOI | 10.11648/j.ajp.20210703.27 |
| Page(s) | 178-181 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2021. Published by Science Publishing Group |
Hirschsprung, Lymph Node Cells, Calretinin, Dakar
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APA Style
Abdou Magib Gaye, Gabriel Nougnignon Comlan Deguenonvo, Ibou Thiam, Sophia Raafa, Marie Joseph Dieme-Ahouidi, et al. (2021). The Value of Calretinin in the Diagnosis of Hirschsprung's Disease in Dakar. American Journal of Pediatrics, 7(3), 178-181. https://doi.org/10.11648/j.ajp.20210703.27
ACS Style
Abdou Magib Gaye; Gabriel Nougnignon Comlan Deguenonvo; Ibou Thiam; Sophia Raafa; Marie Joseph Dieme-Ahouidi, et al. The Value of Calretinin in the Diagnosis of Hirschsprung's Disease in Dakar. Am. J. Pediatr. 2021, 7(3), 178-181. doi: 10.11648/j.ajp.20210703.27
AMA Style
Abdou Magib Gaye, Gabriel Nougnignon Comlan Deguenonvo, Ibou Thiam, Sophia Raafa, Marie Joseph Dieme-Ahouidi, et al. The Value of Calretinin in the Diagnosis of Hirschsprung's Disease in Dakar. Am J Pediatr. 2021;7(3):178-181. doi: 10.11648/j.ajp.20210703.27
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Download@article{10.11648/j.ajp.20210703.27,
author = {Abdou Magib Gaye and Gabriel Nougnignon Comlan Deguenonvo and Ibou Thiam and Sophia Raafa and Marie Joseph Dieme-Ahouidi and Cherif Mouhamed Moustapha Dial},
title = {The Value of Calretinin in the Diagnosis of Hirschsprung's Disease in Dakar},
journal = {American Journal of Pediatrics},
volume = {7},
number = {3},
pages = {178-181},
doi = {10.11648/j.ajp.20210703.27},
url = {https://doi.org/10.11648/j.ajp.20210703.27},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajp.20210703.27},
abstract = {Introduction: Hirschsprung's disease is a congenital anomaly characterised by a rarefaction or absence of ganglion cells associated with schwannian hyperplasia or hypertrophy involving the colon or a segment of the colon. Its diagnosis is based on clinical, radiological and histological evidence. Haematoxylin-eosin examination is used in cases of suspected Hirschsprung's disease. However, in some situations it is insufficient to confirm or refute the diagnosis. Immunohistochemical and histo-enzymatic techniques can be used to demonstrate aganglionosis. The aim of our study is to evaluate the interest of calretinin in the diagnosis of Hirschsprung's disease in Dakar. Materials and methods: This was a retrospective and descriptive study from 1 January 2018 to 31 August 2019. It was conducted at the pathology laboratory of Idrissa Pouye General Hospital where 51 paraffin blocks from suspected Hirschsprung's Disease cases were included. All blocks were immunohistochemically studied with anti-calretinin antibody. The diagnosis of Hirschsprung's disease on standard histological examination was based on the absence of lymph node cells in the submucosal and myenteric plexuses. Calretinin immunoreactivity was shown by nuclear and cytoplasmic labelling of ganglion cells and nerve nets. In Hirschsprung's disease, there is an absence of labelling of the nerve plexuses. Results: The majority of patients (73.5%) were aged 2 years or older with a mean age of 3.4 years. The sex ratio was 2. Biopsies constituted 56.86% of the specimens and surgical specimens 43.14%. Concordance between haematoxylin-eosin examination and calretinin immunohistochemistry was observed in 47 cases (92.15%) and discordance in 4 cases (7.15%). The sensitivity of calretinin was 93.75% and the specificity 89.47%. The kappa index was 0.92. The recto-sigmoid form was the most frequent topographic form observed in 83.3% of patients. Conclusion: Morphological examination with haematoxylin-eosin remains a good diagnostic method for Hirschsprung's disease. Calretinin immunohistochemistry is necessary in equivocal cases, in neonates and infants and in case of superficial biopsies.},
year = {2021}
}
TY - JOUR T1 - The Value of Calretinin in the Diagnosis of Hirschsprung's Disease in Dakar AU - Abdou Magib Gaye AU - Gabriel Nougnignon Comlan Deguenonvo AU - Ibou Thiam AU - Sophia Raafa AU - Marie Joseph Dieme-Ahouidi AU - Cherif Mouhamed Moustapha Dial Y1 - 2021/09/26 PY - 2021 N1 - https://doi.org/10.11648/j.ajp.20210703.27 DO - 10.11648/j.ajp.20210703.27 T2 - American Journal of Pediatrics JF - American Journal of Pediatrics JO - American Journal of Pediatrics SP - 178 EP - 181 PB - Science Publishing Group SN - 2472-0909 UR - https://doi.org/10.11648/j.ajp.20210703.27 AB - Introduction: Hirschsprung's disease is a congenital anomaly characterised by a rarefaction or absence of ganglion cells associated with schwannian hyperplasia or hypertrophy involving the colon or a segment of the colon. Its diagnosis is based on clinical, radiological and histological evidence. Haematoxylin-eosin examination is used in cases of suspected Hirschsprung's disease. However, in some situations it is insufficient to confirm or refute the diagnosis. Immunohistochemical and histo-enzymatic techniques can be used to demonstrate aganglionosis. The aim of our study is to evaluate the interest of calretinin in the diagnosis of Hirschsprung's disease in Dakar. Materials and methods: This was a retrospective and descriptive study from 1 January 2018 to 31 August 2019. It was conducted at the pathology laboratory of Idrissa Pouye General Hospital where 51 paraffin blocks from suspected Hirschsprung's Disease cases were included. All blocks were immunohistochemically studied with anti-calretinin antibody. The diagnosis of Hirschsprung's disease on standard histological examination was based on the absence of lymph node cells in the submucosal and myenteric plexuses. Calretinin immunoreactivity was shown by nuclear and cytoplasmic labelling of ganglion cells and nerve nets. In Hirschsprung's disease, there is an absence of labelling of the nerve plexuses. Results: The majority of patients (73.5%) were aged 2 years or older with a mean age of 3.4 years. The sex ratio was 2. Biopsies constituted 56.86% of the specimens and surgical specimens 43.14%. Concordance between haematoxylin-eosin examination and calretinin immunohistochemistry was observed in 47 cases (92.15%) and discordance in 4 cases (7.15%). The sensitivity of calretinin was 93.75% and the specificity 89.47%. The kappa index was 0.92. The recto-sigmoid form was the most frequent topographic form observed in 83.3% of patients. Conclusion: Morphological examination with haematoxylin-eosin remains a good diagnostic method for Hirschsprung's disease. Calretinin immunohistochemistry is necessary in equivocal cases, in neonates and infants and in case of superficial biopsies. VL - 7 IS - 3 ER -
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