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Evaluation of Remineralization Effect of Various Toothpastes on White Spot Enamel Lesions: An in Vitro Study

Received: 6 March 2022     Accepted: 14 April 2022     Published: 28 April 2022
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Abstract

Despite the high prevalence of tooth-caries and various efforts to prevent it, studies on plant compounds and their anti-caries properties have been largely neglected. Brushing with toothpaste is the most effective way for caries prevention. This study was performed on KREND herbal toothpaste (containing Calendula and Salvia Officinalis), Herbex (containing propolis), and Crest herbal toothpaste. The remineralization effect of these toothpaste brands on carious lesions has not been studied yet. Therefore, we compared the remineralizing effect of Iranian toothpaste and foreign types in this study. Method and materials: 60 caries-free human premolar teeth were collected after extraction for orthodontic purposes. The surface microhardness (SMH) was measured using the Vickers microhardness testing machine at baseline and after four days of immersing in demineralization solution and after treatment. The pH cycle was then performed for 28 days. The teeth were allocated randomly into one of the four groups, group 1 (as the control group and immersed in distilled water), group 2 (treated with KREND herbal solution), group 3 (treated with Crest solution), and group 4 (treated with Herbex solution). Finally, data were analyzed with Repeated Measure ANOVA and SPSS version 18. All study groups' surface micro-hardness measures were decreased after demineralization (P=0.001) and were increased after pH cycling (P<0.001). There wasn't a statistically significant difference in mean Surface micro-hardness changes between study groups (P=0.433). This study showed that the remineralization ratio in study group samples increased after treatment with herbal toothpaste, and the Iranian toothpaste (Krend) and foreign ones had a similar remineralizing effect.

Published in American Journal of Pediatrics (Volume 8, Issue 2)
DOI 10.11648/j.ajp.20220802.17
Page(s) 91-97
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2022. Published by Science Publishing Group

Keywords

White Spot Lesion, Herbal Toothpaste, Krend, Crest, Herbex, Remineralization

References
[1] Sudjalim TR, Woods MG, Manton DJ. Prevention of white spot lesions in orthodontic practice: a contemporary review. Aust Dent J. 2006 Dec; 51 (4): 284-9; quiz 347. doi: 10.1111/j.1834-7819.2006.tb00445.x. PMID: 17256301.
[2] Ballard RW, Hagan JL, Phaup AN, Sarkar N, Townsend JA, Armbruster PC. Evaluation of 3 commercially available materials for resolution of white spot lesions. Am J Orthod Dentofacial Orthop. 2013 Apr; 143 (4 Suppl): S78-84. doi: 10.1016/j.ajodo.2012.08.020. PMID: 23540640.
[3] Kugel G, Arsenault P, Papas A: Treatment modalities for caries management including a new resin infiltration system. Compend Contin Educ Dent 200930 Spec No 3: 1–10; quiz 11-12.
[4] Retraction: Role of casein phosphopeptide amorphous calcium phosphate in remineralization of white spot lesions and inhibition of Streptococcus mutans? J Conserv Dent. 2016 Mar-Apr; 19 (2): 198. doi: 10.4103/0972-0707.178710. PMID: 27099432; PMCID: PMC4815554.
[5] Amaechi BT, van Loveren C. Fluorides and non-fluoride remineralization systems. Monogr Oral Sci. 2013; 23: 15-26. doi: 10.1159/000350458. Epub 2013 Jun 28. PMID: 23817057.
[6] Pannuti CM, Mattos JP, Ranoya PN, Jesus AM, Lotufo RF, Romito GA. Clinical effect of a herbal dentifrice on the control of plaque and gingivitis: a double-blind study. Pesqui Odontol Bras. 2003 Oct-Dec; 17 (4): 314-8. doi: 10.1590/s1517-74912003000400004. Epub 2004 Apr 19. PMID: 15107912.
[7] Beheshti-Rouy M, Azarsina M, Rezaie-Soufi L, Alikhani MY, Roshanaie G, Komaki S. The antibacterial effect of sage extract (Salvia officinalis) mouthwash against Streptococcus mutans in dental plaque: a randomized clinical trial. Iran J Microbiol. 2015 Jun; 7 (3): 173-7. PMID: 26668706; PMCID: PMC4676988.
[8] S VK. Propolis in dentistry and oral cancer management. N Am J Med Sci. 2014 Jun; 6 (6): 250-9. doi: 10.4103/1947-2714.134369. PMID: 25006559; PMCID: PMC4083525.
[9] Santos FA, Bastos EM, Uzeda M, Carvalho MA, Farias LM, Moreira ES, Braga FC. Antibacterial activity of Brazilian propolis and fractions against oral anaerobic bacteria. J Ethnopharmacol. 2002 Apr; 80 (1): 1-7. doi: 10.1016/s0378-8741(02)00003-x. PMID: 11891080.
[10] Silici S, Koç NA, Ayangil D, Cankaya S. Antifungal activities of propolis collected by different races of honeybees against yeasts isolated from patients with superficial mycoses. J Pharmacol Sci. 2005 Sep; 99 (1): 39-44. doi: 10.1254/jphs.fpe05002x. Epub 2005 Sep 1. PMID: 16141640.
[11] Torwane NA, Hongal S, Goel P, Chandrashekar BR, Jain M, Saxena E. A clinical efficacy of 30% ethenolic extract of Indian propolis and Recaldent™ in management of dentinal hypersensitivity: A comparative randomized clinical trial. Eur J Dent. 2013 Oct; 7 (4): 461-468. doi: 10.4103/1305-7456.120675. PMID: 24932122; PMCID: PMC4053672.
[12] Hu F, Hepburn HR, Li Y, Chen M, Radloff SE, Daya S. Effects of ethanol and water extracts of propolis (bee glue) on acute inflammatory animal models. J Ethnopharmacol. 2005 Sep 14; 100 (3): 276-83. doi: 10.1016/j.jep.2005.02.044. PMID: 15899563.
[13] Liao HF, Chen YY, Liu JJ, Hsu ML, Shieh HJ, Liao HJ, Shieh CJ, Shiao MS, Chen YJ. Inhibitory effect of caffeic acid phenethyl ester on angiogenesis, tumor invasion, and metastasis. J Agric Food Chem. 2003 Dec 31; 51 (27): 7907-12. doi: 10.1021/jf034729d. PMID: 14690372.
[14] Nassar SA, Mohamed AH, Soufy H, Nasr SM, Mahran KM. Immunostimulant effect of Egyptian propolis in rabbits. Scientific World Journal. 2012; 901516. doi: 10.1100/2012/901516. Epub 2012 May 2. PMID: 22654648; PMCID: PMC3354672.
[15] Sookto T, Srithavaj T, Thaweboon S, Thaweboon B, Shrestha B. In vitro effects of Salvia officinalis L. essential oil on Candida albicans. Asian Pac J Trop Biomed. 2013 May; 3 (5): 376-80. doi: 10.1016/S2221-1691(13)60080-5. PMID: 23646301; PMCID: PMC3642447.
[16] Neto FR, Maeda F, Turssi C, Serra M. Potential agents to control enamel caries-like lesions. Journal of dentistry. 2009; 37 (10): 786-90.
[17] Rehder Neto FC, Maeda FA, Turssi CP, Serra MC. Potential agents to control enamel caries-like lesions. J Dent. 2009 Oct; 37 (10): 786-90. doi: 10.1016/j.jdent.2009.06.008. Epub 2009 Jun 18. PMID: 19595495.
[18] Cochran MA. Art and science. Oper Dent. 2007 May-Jun; 32 (3): 205-6. doi: 10.2341/1559-2863(2007)32 [205: AAS] 2.0.CO; 2. PMID: 17555169.
[19] Harris NO, Garcia-Godoy F, NatheCN. primary preventive dentistry.6th Ed. Pearson Education inc. 2004. Chap 1, 3, 6, 13, 52, 62, 103-124.
[20] Ismail A. I, Hassan H, Sohn W. Dental caries in the second millennium. J of Dent Edu. October 2001: 953-959.
[21] Mehrdad K. Dental caries and prevention. Shahid Beheshti University of Medical sciences. 1th Ed. Chapter: 40, 41.
[22] Wieckiewicz W, Miernik M, Wieckiewicz M, Morawiec T. Does Propolis Help to Maintain Oral Health? Evidence-based Complementary and Alternative Medicine: eCAM. 2013: 351062.
[23] Coutinho A. Honeybee propolis extract in periodontal treatment: A clinical and microbiological study of propolis in periodontal treatment. Indian J Dent Res 2012: 23: 294.
[24] Koo H, Rosalen PL, Cury JA, Park YK, Bowen WH. Effects of cCompounds Found in Propolis on Streptococcus mutans Growth and on Glucosyltransferase Activity. Antimicrobial Agents and Chemotherapy. 2002: 46 (5): 1302-1309.
[25] Ucan MC, Koparal M, AgacayakS, Gunay A, Ozgoz M, Atilgan S, et al. Influence of caffeic acid phenethyl ester on bone healing in a rat model. J Int Med Res. 2013: 41 (5): 1648-1654.
[26] Magro Filho O, de Carvalho AC. Application of propolis to dental sockets and skin wounds. J Nihon Univ Sch Dent. 1990 Mar; 32 (1): 4-13. doi: 10.2334/josnusd1959.32.4. PMID: 2345377.
[27] Martin MP, Pileggi R. A quantitative analysis of Propolis: a promising new storage media following avulsion. Dent Traumatol. 2004 Apr; 20 (2): 85-9. doi: 10.1111/j.1600-4469.2004.00233.x. PMID: 15025690.
[28] Barak S, Katz J. The effect of Breezy candy on halitosis: a double-blind, controlled, and randomized study. Quintessence Int. 2012 Apr; 43 (4): 313-7. PMID: 22532945.
[29] Santos FA, Bastos EM, Rodrigues PH, de Uzeda M, de Cavalho MA, FariasLde M, et al. Susceotibility of Prevotellaintermedial / Prevotellanigrescens (and Porphyromonasgingivalis) to Propolis (bee glue) and other antimicrobial agents. Anaerobe. 2002: 8 (1): 9-15.
[30] Anauate-Netto C, Anido-Anido A, Leegoy HR, Matsumoto R, Alonso RC, Marcucci MC, et al. Randomised, double-blind, placebo-controlled clinical trial on the effect of propolis and chlorhexidine mouthrinses on gingivitis. Braz Dent Sci. 2014: 17 (1): 11-15.
[31] Pereira EM, da Silva JL, Silva FF, De Luca MP, Ferreira EF, Lorentz TC, Santos VR. Clinical Evidence of the Efficacy of a Mouthwash Containing Propolis for the Control of Plaque and Gingivitis: A Phase II Study. Evid Based Complement Alternat Med. 2011: 750249. doi: 10.1155/2011/750249. Epub 2011 Mar 31. PMID: 21584253; PMCID: PMC3092688.
[32] Hogal S, Torwane NA, Goel P, Chandrashekar B. the effect of of 30% ethenolic extract of indian propolis on replica of human dentin compared against commercialiy available desensitizing agent: A methodological SEM study in vitro. Pharmacognosy Res. 2014: 6 (2): 113-119.
[33] Madhavan S, Nayak M, Shenoy A, Shetty R, Prasad K. Dentinal hypersensitivity: A comparative clinical evaluation of CPP-ACP F, sodium fluoride, propolis, and placebo. J Conserv Dent. 2012 Oct; 15 (4): 315-8. doi: 10.4103/0972-0707.101882. PMID: 23112475; PMCID: PMC3482741.
[34] Purra AR, Mushtaq M, Acharya SR, Saraswati V. A comparative evaluation of propolis and 5.0% potassium nitrate as a dentine desensitizer: A clinical study. J Indian Soc Periodontol. 2014 Jul; 18 (4): 466-71. doi: 10.4103/0972-124X.138695. PMID: 25210261; PMCID: PMC4158588.
[35] Faria RL, Cardoso LM, Akisue G, Pereira CA, Junqueira JC, Jorge AO, Santos Júnior PV. Antimicrobial activity of Calendula officinalis, Camellia sinensis and chlorhexidine against the adherence of microorganisms to sutures after extraction of unerupted third molars. J Appl Oral Sci. 2011 Oct; 19 (5): 476-82. doi: 10.1590/s1678-77572011000500007. PMID: 21986652; PMCID: PMC3984193.
[36] Mahyari S, Mahyari B, Emami SA, Malaekeh-Nikouei B, Jahanbakhsh SP, Sahebkar A, Mohammadpour AH. Evaluation of the efficacy of a polyherbal mouthwash containing Zingiber officinale, Rosmarinus officinalis and Calendula officinalis extracts in patients with gingivitis: A randomized double-blind placebo-controlled trial. Complement Ther Clin Pract. 2016 Feb; 22: 93-8. doi: 10.1016/j.ctcp.2015.12.001. Epub 2015 Dec 10. PMID: 26850813.
[37] Anusavice K. Mechanical properties of dental materials. In: AnusaviceKenneth J: Phillips Science of dental material. 11th Ed Philadephia: WBSaunders 2003: 96-98.
[38] Byeon SM, Lee MH, Bae TS. The effect of different fluoride application methods on the remineralization of initial carious lesions. Restor Dent Endod. 2016 May; 41 (2): 121-9. doi: 10.5395/rde.2016.41.2.121. Epub 2016 May 10. PMID: 27200280; PMCID: PMC4868875.
[39] Shetty KP, Satish SV, Gouda V, Badade AR, Gouda B, Patil S. Comparative evaluation and effect of organic and inorganic fluoride dentifrices on enamel microhardness: An in vitro study. J Int Soc Prev Community Dent. 2016 Mar-Apr; 6 (2): 130-3. doi: 10.4103/2231-0762.178751. PMID: 27114952; PMCID: PMC4820572.
[40] Damle SG, Bector A, Damle D, Kaur S. Effect of dentifrices on their remineralizing potential in artificial carious lesions: An in situ study. Dent Res J (Isfahan). 2016 Jan-Feb; 13 (1): 74-9. doi: 10.4103/1735-3327.174721. PMID: 26962320; PMCID: PMC4770475.
[41] Salehzadeh Esfahani K, Mazaheri R, Pishevar L. Effects of Treatment with Various Remineralizing Agents on the Microhardness of Demineralized Enamel Surface. J Dent Res Dent Clin Dent Prospects. 2015 Fall; 9 (4): 239-45. doi: 10.15171/joddd.2015.043. Epub 2015 Dec 30. PMID: 26889361; PMCID: PMC4753033.
[42] Elkassas D, Arafa A. Remineralizing efficacy of different calcium-phosphate and fluoride based delivery vehicles on artificial caries like enamel lesions. J Dent. 2014 Apr; 42 (4): 466-74. doi: 10.1016/j.jdent.2013.12.017. Epub 2014 Jan 9. PMID: 24412586.
[43] Shetty S, Hegde MN, Bopanna TP. Enamel remineralization assessment after treatment with three different remineralizing agents using surface microhardness: An in vitro study. J Conserv Dent. 2014 Jan; 17 (1): 49-52. doi: 10.4103/0972-0707.124136. PMID: 24554861; PMCID: PMC3915386.
[44] Mehta AB, Kumari V, Jose R, Izadikhah V. Remineralization potential of bioactive glass and casein phosphopeptide-amorphous calcium phosphate on initial carious lesion: An in-vitro pH-cycling study. J Conserv Dent. 2014 Jan; 17 (1): 3-7. doi: 10.4103/0972-0707.124085. PMID: 24554851; PMCID: PMC3915381.
[45] Milly H, Festy F, Watson TF, Thompson I, Banerjee A. Enamel white spot lesions can remineralise using bio-active glass and polyacrylic acid-modified bio-active glass powders. J Dent. 2014 Feb; 42 (2): 158-66. doi: 10.1016/j.jdent.2013.11.012. Epub 2013 Nov 25. PMID: 24287257.
[46] Malekafzali B, Ekrami M, Mirfasihi A, Abdolazimi Z. Remineralizing Effect of Child Formula Dentifrices on Artificial Enamel Caries Using a pH Cycling Model. J Dent (Tehran). 2015 Jan; 12 (1): 11-7. PMID: 26005449; PMCID: PMC4436322.
[47] Zhou SL, Zhou J, Watanabe S, Watanabe K, Wen LY, Xuan K. In vitro study of the effects of fluoride-releasing dental materials on remineralization in an enamel erosion model. J Dent. 2012 Mar; 40 (3): 255-63. doi: 10.1016/j.jdent.2011.12.016. Epub 2011 Dec 28. PMID: 22227269.
[48] Vahid Golpayegani M, Sohrabi A, Biria M, Ansari G. Remineralization Effect of Topical NovaMin Versus Sodium Fluoride (1.1%) on Caries-Like Lesions in Permanent Teeth. J Dent (Tehran). 2012 Winter; 9 (1): 68-75. Epub 2012 Mar 31. PMID: 22924104; PMCID: PMC3422065.
[49] Karlinsey RL, Mackey AC, Walker TJ, Frederick KE, Blanken DD, Flaig SM, Walker ER. In vitro remineralization of human and bovine white-spot enamel lesions by NaF dentifrices: A pilot study. J Dent Oral Hyg. 2011 Feb; 3 (2): 22-29. PMID: 21643437; PMCID: PMC3105352.
[50] Cheng L, Li J, Hao Y, Zhou X. Effect of compounds of Galla chinensis and their combined effects with fluoride on remineralization of initial enamel lesion in vitro. J Dent. 2008 May; 36 (5): 369-73. doi: 10.1016/j.jdent.2008.01.011. Epub 2008 Mar 4. PMID: 18308448.
[51] Bandekar S, Patil S, Dudulwar D, Moogi PP, Ghosh S, Kshirsagar S. Remineralization potential of fluoride, amorphous calcium phosphate-casein phosphopeptide, and combination of hydroxylapatite and fluoride on enamel lesions: An in vitro comparative evaluation. J Conserv Dent. 2019 May-Jun; 22 (3): 305-309. doi: 10.4103/JCD.JCD_13_19. PMID: 31367119; PMCID: PMC6632629.
[52] J. Hong, B. I. Kim, H. K. Kwon and C. H. Choi. Remineralization Effects of Herbal Extracts with Fluoride on Artificial Carious Enamel.
[53] Bereket C, Özan F, Şener İ, Tek M, Altunkaynak BZ, Semirgin SU, Şenel E, Özdemir M. Propolis accelerates the consolidation phase in distraction osteogenesis. J Craniofac Surg. 2014 Sep; 25 (5): 1912-6. doi: 10.1097/SCS.0000000000000946. PMID: 25203585.
[54] Kamburoğlu K, Özen T. Analgesic effect of Anatolian propolis in mice. Agri. 2011 Apr; 23 (2): 47-50. PMID: 21644103.
[55] Brailo V, Boras VV, Alajbeg I, Juras V. Delayed contact sensitivity on the lips and oral mucosa due to propolis-case report. Med Oral Patol Oral Cir Bucal. 2006 Jul 1; 11 (4): E303-4. PMID: 16816812.
[56] Bagheri H, Namdar F, Hosseini Rivandi M, Asadi S. Color properties of artificial white spot lesions treated by experimental resin infiltrants containing bioactive glass and nano-fluorohydroxyapatite. Int J Esthet Dent. 2020; 15 (3): 334-343. PMID: 32760927.
[57] Mohan AG, Ebenezar AVR, Ghani MF, Martina L, Narayanan A, Mony B. Surface and mineral changes of enamel with different remineralizing agents in conjunction with carbon-dioxide laser. Eur J Dent. 2014 Jan; 8 (1): 118-123. doi: 10.4103/1305-7456.126264. PMID: 24966758; PMCID: PMC4054023.
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    Masoumeh Moslemi, Mehrnaz Mahmodabadi, Zahra Bastani, Zahra Khalili Sadrabad, Faezeh Sadat Faghihi, et al. (2022). Evaluation of Remineralization Effect of Various Toothpastes on White Spot Enamel Lesions: An in Vitro Study. American Journal of Pediatrics, 8(2), 91-97. https://doi.org/10.11648/j.ajp.20220802.17

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    Masoumeh Moslemi; Mehrnaz Mahmodabadi; Zahra Bastani; Zahra Khalili Sadrabad; Faezeh Sadat Faghihi, et al. Evaluation of Remineralization Effect of Various Toothpastes on White Spot Enamel Lesions: An in Vitro Study. Am. J. Pediatr. 2022, 8(2), 91-97. doi: 10.11648/j.ajp.20220802.17

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    AMA Style

    Masoumeh Moslemi, Mehrnaz Mahmodabadi, Zahra Bastani, Zahra Khalili Sadrabad, Faezeh Sadat Faghihi, et al. Evaluation of Remineralization Effect of Various Toothpastes on White Spot Enamel Lesions: An in Vitro Study. Am J Pediatr. 2022;8(2):91-97. doi: 10.11648/j.ajp.20220802.17

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  • @article{10.11648/j.ajp.20220802.17,
      author = {Masoumeh Moslemi and Mehrnaz Mahmodabadi and Zahra Bastani and Zahra Khalili Sadrabad and Faezeh Sadat Faghihi and Zahra Shadkar},
      title = {Evaluation of Remineralization Effect of Various Toothpastes on White Spot Enamel Lesions: An in Vitro Study},
      journal = {American Journal of Pediatrics},
      volume = {8},
      number = {2},
      pages = {91-97},
      doi = {10.11648/j.ajp.20220802.17},
      url = {https://doi.org/10.11648/j.ajp.20220802.17},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajp.20220802.17},
      abstract = {Despite the high prevalence of tooth-caries and various efforts to prevent it, studies on plant compounds and their anti-caries properties have been largely neglected. Brushing with toothpaste is the most effective way for caries prevention. This study was performed on KREND herbal toothpaste (containing Calendula and Salvia Officinalis), Herbex (containing propolis), and Crest herbal toothpaste. The remineralization effect of these toothpaste brands on carious lesions has not been studied yet. Therefore, we compared the remineralizing effect of Iranian toothpaste and foreign types in this study. Method and materials: 60 caries-free human premolar teeth were collected after extraction for orthodontic purposes. The surface microhardness (SMH) was measured using the Vickers microhardness testing machine at baseline and after four days of immersing in demineralization solution and after treatment. The pH cycle was then performed for 28 days. The teeth were allocated randomly into one of the four groups, group 1 (as the control group and immersed in distilled water), group 2 (treated with KREND herbal solution), group 3 (treated with Crest solution), and group 4 (treated with Herbex solution). Finally, data were analyzed with Repeated Measure ANOVA and SPSS version 18. All study groups' surface micro-hardness measures were decreased after demineralization (P=0.001) and were increased after pH cycling (P<0.001). There wasn't a statistically significant difference in mean Surface micro-hardness changes between study groups (P=0.433). This study showed that the remineralization ratio in study group samples increased after treatment with herbal toothpaste, and the Iranian toothpaste (Krend) and foreign ones had a similar remineralizing effect.},
     year = {2022}
    }
    

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  • TY  - JOUR
    T1  - Evaluation of Remineralization Effect of Various Toothpastes on White Spot Enamel Lesions: An in Vitro Study
    AU  - Masoumeh Moslemi
    AU  - Mehrnaz Mahmodabadi
    AU  - Zahra Bastani
    AU  - Zahra Khalili Sadrabad
    AU  - Faezeh Sadat Faghihi
    AU  - Zahra Shadkar
    Y1  - 2022/04/28
    PY  - 2022
    N1  - https://doi.org/10.11648/j.ajp.20220802.17
    DO  - 10.11648/j.ajp.20220802.17
    T2  - American Journal of Pediatrics
    JF  - American Journal of Pediatrics
    JO  - American Journal of Pediatrics
    SP  - 91
    EP  - 97
    PB  - Science Publishing Group
    SN  - 2472-0909
    UR  - https://doi.org/10.11648/j.ajp.20220802.17
    AB  - Despite the high prevalence of tooth-caries and various efforts to prevent it, studies on plant compounds and their anti-caries properties have been largely neglected. Brushing with toothpaste is the most effective way for caries prevention. This study was performed on KREND herbal toothpaste (containing Calendula and Salvia Officinalis), Herbex (containing propolis), and Crest herbal toothpaste. The remineralization effect of these toothpaste brands on carious lesions has not been studied yet. Therefore, we compared the remineralizing effect of Iranian toothpaste and foreign types in this study. Method and materials: 60 caries-free human premolar teeth were collected after extraction for orthodontic purposes. The surface microhardness (SMH) was measured using the Vickers microhardness testing machine at baseline and after four days of immersing in demineralization solution and after treatment. The pH cycle was then performed for 28 days. The teeth were allocated randomly into one of the four groups, group 1 (as the control group and immersed in distilled water), group 2 (treated with KREND herbal solution), group 3 (treated with Crest solution), and group 4 (treated with Herbex solution). Finally, data were analyzed with Repeated Measure ANOVA and SPSS version 18. All study groups' surface micro-hardness measures were decreased after demineralization (P=0.001) and were increased after pH cycling (P<0.001). There wasn't a statistically significant difference in mean Surface micro-hardness changes between study groups (P=0.433). This study showed that the remineralization ratio in study group samples increased after treatment with herbal toothpaste, and the Iranian toothpaste (Krend) and foreign ones had a similar remineralizing effect.
    VL  - 8
    IS  - 2
    ER  - 

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Author Information
  • Pediatric Department, Faculty of Dentistry, Shahid Beheshti University of Medical Sciences, Tehran, Iran

  • Pediatric Department, Faculty of Dentistry, Shahid Beheshti University of Medical Sciences, Tehran, Iran

  • Pediatric Department, Faculty of Dentistry, Shahid Beheshti University of Medical Sciences, Tehran, Iran

  • Pediatric Department, Faculty of Dentistry, Shahid Beheshti University of Medical Sciences, Tehran, Iran

  • Pediatric Department, Faculty of Dentistry, Shahid Beheshti University of Medical Sciences, Tehran, Iran

  • Pediatric Department, Faculty of Dentistry, Shahid Beheshti University of Medical Sciences, Tehran, Iran

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