The study evaluated the antimalarial activities of the methanolic extract of Trichilia heudelotii leaf in mice, identified the most active partitioned and chromatographic fractions with a view to providing information on the scientific basis of the ethnomedicinal uses of the plant in treatment of malaria. The air-dried leaf of T. heudelotii was milled into powder and extracted with methanol. The median lethal dose (LD50) was determined according to Lorke’s method. The extract was tested against chloroquine-sensitive strain of Plasmodium bergheii bergheii NK-65 at doses of 100–800 mg/kg using the chemosuppressive antimalarial model while distilled water (0.2 mL) and chloroquine (10 mg/kg) were used as the negative and positive controls, respectively. The methanol extract was successively partitioned to obtain n-hexane (THH), dichloromethane (THD), ethylacetate (THE), n-butanol (THB) and aqueous fractions (THA) which were each tested at 50, 100 and 200 mg/kg similarly. The most active THH was successively chromatographed and the most active fractions THH2B and THH3B identified. The percentage chemosuppression and percentage survivor in mice was used as a measure of the antiplasmodial activities of the extract, partitioned and column fractions. The LD50 of T. heudelotii leaf extract was greater than 5000 mg/kg. It gave the highest chemosuppression of 88.7% at 200mg/kg, the n-hexane partitioned fraction (THH) gave percentage chemosuppression of 66% at 50mg/kg while subsequent column fractions, THH2B and THH3B gave 77 and 74% respectively. The chemosuppressive activities of T. heudelotii leaf was obviously retained during subsequent purification with the fractions demonstrating good% chemosuppression and percentage survivor profile as to contain the antimalarial constituents of the plant. Gas Chromatography- Mass Spectroscopy of THH3B, the most active column fraction suggested 6, 10, 14-trimethyl-2-pentadecanone, methyl palmitate and 11-Octadecenoic acid, methyl ester that were the major compounds identified in the fraction as possible antimalarial compounds in the plant.
| Published in | American Journal of Bioscience and Bioengineering (Volume 11, Issue 5) |
| DOI | 10.11648/j.bio.20231105.12 |
| Page(s) | 66-74 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2023. Published by Science Publishing Group |
T. heudelotii, Plasmodium bergheii bergheii NK-65, Meliaceae, Chemosuppressive, Antimalarial
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APA Style
Sulaiman Muhammed Olatunji, Odediran Samuel Akintunde, Aladesanmi Joseph Adetunji. (2023). In vivo Antiplasmodial Studies on Trichilia heudelotii Planch ex. Oliver (Meliaceae) Leaf. American Journal of Bioscience and Bioengineering, 11(5), 66-74. https://doi.org/10.11648/j.bio.20231105.12
ACS Style
Sulaiman Muhammed Olatunji; Odediran Samuel Akintunde; Aladesanmi Joseph Adetunji. In vivo Antiplasmodial Studies on Trichilia heudelotii Planch ex. Oliver (Meliaceae) Leaf. Am. J. BioSci. Bioeng. 2023, 11(5), 66-74. doi: 10.11648/j.bio.20231105.12
AMA Style
Sulaiman Muhammed Olatunji, Odediran Samuel Akintunde, Aladesanmi Joseph Adetunji. In vivo Antiplasmodial Studies on Trichilia heudelotii Planch ex. Oliver (Meliaceae) Leaf. Am J BioSci Bioeng. 2023;11(5):66-74. doi: 10.11648/j.bio.20231105.12
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Download@article{10.11648/j.bio.20231105.12,
author = {Sulaiman Muhammed Olatunji and Odediran Samuel Akintunde and Aladesanmi Joseph Adetunji},
title = {In vivo Antiplasmodial Studies on Trichilia heudelotii Planch ex. Oliver (Meliaceae) Leaf},
journal = {American Journal of Bioscience and Bioengineering},
volume = {11},
number = {5},
pages = {66-74},
doi = {10.11648/j.bio.20231105.12},
url = {https://doi.org/10.11648/j.bio.20231105.12},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.bio.20231105.12},
abstract = {The study evaluated the antimalarial activities of the methanolic extract of Trichilia heudelotii leaf in mice, identified the most active partitioned and chromatographic fractions with a view to providing information on the scientific basis of the ethnomedicinal uses of the plant in treatment of malaria. The air-dried leaf of T. heudelotii was milled into powder and extracted with methanol. The median lethal dose (LD50) was determined according to Lorke’s method. The extract was tested against chloroquine-sensitive strain of Plasmodium bergheii bergheii NK-65 at doses of 100–800 mg/kg using the chemosuppressive antimalarial model while distilled water (0.2 mL) and chloroquine (10 mg/kg) were used as the negative and positive controls, respectively. The methanol extract was successively partitioned to obtain n-hexane (THH), dichloromethane (THD), ethylacetate (THE), n-butanol (THB) and aqueous fractions (THA) which were each tested at 50, 100 and 200 mg/kg similarly. The most active THH was successively chromatographed and the most active fractions THH2B and THH3B identified. The percentage chemosuppression and percentage survivor in mice was used as a measure of the antiplasmodial activities of the extract, partitioned and column fractions. The LD50 of T. heudelotii leaf extract was greater than 5000 mg/kg. It gave the highest chemosuppression of 88.7% at 200mg/kg, the n-hexane partitioned fraction (THH) gave percentage chemosuppression of 66% at 50mg/kg while subsequent column fractions, THH2B and THH3B gave 77 and 74% respectively. The chemosuppressive activities of T. heudelotii leaf was obviously retained during subsequent purification with the fractions demonstrating good% chemosuppression and percentage survivor profile as to contain the antimalarial constituents of the plant. Gas Chromatography- Mass Spectroscopy of THH3B, the most active column fraction suggested 6, 10, 14-trimethyl-2-pentadecanone, methyl palmitate and 11-Octadecenoic acid, methyl ester that were the major compounds identified in the fraction as possible antimalarial compounds in the plant.},
year = {2023}
}
TY - JOUR T1 - In vivo Antiplasmodial Studies on Trichilia heudelotii Planch ex. Oliver (Meliaceae) Leaf AU - Sulaiman Muhammed Olatunji AU - Odediran Samuel Akintunde AU - Aladesanmi Joseph Adetunji Y1 - 2023/09/27 PY - 2023 N1 - https://doi.org/10.11648/j.bio.20231105.12 DO - 10.11648/j.bio.20231105.12 T2 - American Journal of Bioscience and Bioengineering JF - American Journal of Bioscience and Bioengineering JO - American Journal of Bioscience and Bioengineering SP - 66 EP - 74 PB - Science Publishing Group SN - 2328-5893 UR - https://doi.org/10.11648/j.bio.20231105.12 AB - The study evaluated the antimalarial activities of the methanolic extract of Trichilia heudelotii leaf in mice, identified the most active partitioned and chromatographic fractions with a view to providing information on the scientific basis of the ethnomedicinal uses of the plant in treatment of malaria. The air-dried leaf of T. heudelotii was milled into powder and extracted with methanol. The median lethal dose (LD50) was determined according to Lorke’s method. The extract was tested against chloroquine-sensitive strain of Plasmodium bergheii bergheii NK-65 at doses of 100–800 mg/kg using the chemosuppressive antimalarial model while distilled water (0.2 mL) and chloroquine (10 mg/kg) were used as the negative and positive controls, respectively. The methanol extract was successively partitioned to obtain n-hexane (THH), dichloromethane (THD), ethylacetate (THE), n-butanol (THB) and aqueous fractions (THA) which were each tested at 50, 100 and 200 mg/kg similarly. The most active THH was successively chromatographed and the most active fractions THH2B and THH3B identified. The percentage chemosuppression and percentage survivor in mice was used as a measure of the antiplasmodial activities of the extract, partitioned and column fractions. The LD50 of T. heudelotii leaf extract was greater than 5000 mg/kg. It gave the highest chemosuppression of 88.7% at 200mg/kg, the n-hexane partitioned fraction (THH) gave percentage chemosuppression of 66% at 50mg/kg while subsequent column fractions, THH2B and THH3B gave 77 and 74% respectively. The chemosuppressive activities of T. heudelotii leaf was obviously retained during subsequent purification with the fractions demonstrating good% chemosuppression and percentage survivor profile as to contain the antimalarial constituents of the plant. Gas Chromatography- Mass Spectroscopy of THH3B, the most active column fraction suggested 6, 10, 14-trimethyl-2-pentadecanone, methyl palmitate and 11-Octadecenoic acid, methyl ester that were the major compounds identified in the fraction as possible antimalarial compounds in the plant. VL - 11 IS - 5 ER -
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