Phytochemical Screening and <i>in Vitro</i> Antimicrobial Activity of <i>Waltheria Indica Linn</i> Leaf Extracts

Okwute Simon Koma; Olakunle Ayodeji Fatokun; Orishadipe Abayomi Theophilus

doi:doi:10.11648/j.bs.20170305.11

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Phytochemical Screening and in Vitro Antimicrobial Activity of Waltheria Indica Linn Leaf Extracts

Okwute Simon Koma, Olakunle Ayodeji Fatokun, Orishadipe Abayomi Theophilus

Published in Biomedical Sciences (Volume 3, Issue 5)

Received: 20 August 2017 Accepted: 31 August 2017 Published: 6 October 2017

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Abstract

The plant, Waltherica indica Linn is known to possess medicinal properties according to African folklore. Scientific verification of its bioactive constituents backing its use for the treatment of upper respiratory tract infections is limited. This study aimed at evaluating the phytochemical compounds present in the crude leaf extracts as well as identify their pathogen-specific antagonistic activities. The results obtained revealed that alkaloids, cardiac glycosides, phenolic acids, saponins, steroids, tannins and terpenoids were present in the extracts. The results also revealed that the zones of inhibition from the acidic, basic, neutral polar and non-polar fractions ranged between 24-28mm against the organisms (Staphylococcus aureus, Streptococcus pneumonia, Streptococcus pyrogens, Klebsciella pneumonia, and Candida krusei). The Minimal Inhibitory Concentration (MICs) of the crude, acidic, basic, neutral polar and non-polar fractions of the leaf was recorded at 2.5mg/ml and 0.5mg/ml respectively. The results obtained suggested that the leaf of the studied plant possess strong antimicrobial activities against selected bacterial and fungal species with direct correlation to the actions of their phyto-constituents.

Published in	Biomedical Sciences (Volume 3, Issue 5)
DOI	10.11648/j.bs.20170305.11
Page(s)	86-93
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2017. Published by Science Publishing Group

Keywords

Waltherica Indica Linn Leaf, Phytochemicals, Anti-microbial Activity

References

[1]	Doughart J. H., and Okafor B., (2007). Anti-microbial activity of Senna alata (Linn). East and Central Africa Journal of Pharmaceutical Sciences, 10: 17-21.
[2]	Okwute S. K., Olajide O. O., Etuk-Udo G., and Orishadipe A. T., (2016). Phytochemical Screening, In-vitro Antimicrobial Activity and Antioxidant Characteristics of Tetrapleura tetraptera Extracts. European Journal of Medicinal Plants, 17: 1-10.
[3]	Shakeri A., Hazeri N., Vlizadeh J., Ghasemi A., and Tavallaei F., (2012). Phytochemical Screening, Antimicrobial and Antioxidant Activity of Anabasis aphylla L. Extracts. Kragujevac Journal of Science, 34: 71-78.
[4]	Ahameethunisa A., and Hoper W., (2010). Antibacterial activity of Artemisia nilagirica leaf extract against clinical and phytopathogenic bacteria. BMC Complementary and Alternative Medicine, 10: 6-11.
[5]	Bachir R. G., and Benali M., (2012). Antibacterial activity of the essential oils from the leaves of Eucalyptus globulus against Escherichia coli and Staphylococcus aureus. Asian Pacific Journal of Tropical Biomedicine, 2: 739-742.
[6]	Uttu A. J., Sallau M. S., Hamisu I., Mubarak B. D., and Abdullahi Y. I., (2015). Phytochemical and antimicrobial screening of stem bark extracts from Glossonema boveanum (Decne). Journal of Pharmacognosy and Phytochemistry, 4: 86-88.
[7]	Seyyednejad S. M., Motamedi H., Vafei M., and Bakhtiari A., (2014). The antibacterial activity of Cassia fistula organic extracts. Jundishapur Journal of Microbiology, 7: 1-5.
[8]	Chanda S., and Rakholiya K., (2011). Combination therapy: Synergism between natural plant extracts and antibiotics against infectious diseases. Science against microbial pathogens: communicating current research and technological advances A. Méndez-Vilas (Ed.).
[9]	Coates A., Hu Y., Bax R., and Page C., (2002). The future challenges facing the development of new antimicrobial drugs. Nature reviews, 1: 895-910.
[10]	Bardia A., Nisly N. L., Zimmerman M. B., Gryzlak B. M., and Wallace R. B., (2007). Use of herbs among adults based on evidence-based indications: findings from the National Health Interview Survey. Mayo Clinic Proceedings, 82: 561–566.
[11]	Lewis K., and Ausubel F. M., (2006). Prospects of plant derived antibacterials. Nature Biotechnology, 24: 1504-1507.
[12]	Kamboj V. P., (2000). Herbal medicine. Current Science, 78: 35–51.
[13]	Balunas M. J., and Kinghorn A. D., (2005). Drug discovery from medicinal plants. Life science Journal, 78: 431-441.
[14]	Tanaka J. C. A., da Silva C. C., de Oliveira A. J. B., Nakamura C. V., and Dias-Filho B. P., (2006). Antibacterial activity of indole alkaloids from Aspidosperma ramiflorum. Brazilian Journal of Medical Biology Research, 39: 387-391.
[15]	Gautam R., Saklani A., and Jachak S. M., (2007). Indian medicinal plants as a source of antimycobacterial agents. Journal of Ethnopharmacology, 110: 200-234.
[16]	Abiramasundari P., Priya V., Jeyanthi G. P., and Gayathri-Devi S., (2011). Evaluation of the Antibacterial activity of Cocculus hirsutus. Hygeia. Journal for Drugs and Medicines, 3: 26-31.
[17]	Abdallah E. M., (2011). Plants: An alternative source for antimicrobials. Journal of Applied Pharmaceutical Science, 1: 16-20.
[18]	McKay D. L., and Blumberg J. B., (2006). A review of the bioactivity and potential health benefits of peppermint tea (Mentha piperita L.). Phytotherapy Research, 20: 619–633.
[19]	Alma M. H., Mavi A., Yildirim A., Digrak M., and Hirata T., (2003). Screening chemical composition and in vitro antioxidant and antimicrobial activities of the essential oils from Origanum syriacum L. growing in Turkey. Biological and Pharmaceutical Bulletin, 26: 1725–1729.
[20]	Soylu S., Yigitbas H., Soylu E. M., and Kurt S., (2007). Antifungal effects of essential oils from oregano and fennel on Sclerotinia sclerotiorum. Journal of Applied Microbiology, 103: 1021–1030.
[21]	Boskabady M. H., and Jandaghi P., (2003). Relaxant effects of carvacrol on guinea pig tracheal chains and its possible mechanisms. Pharmazie, 58: 661–663.
[22]	Lu X. Q., Tang F. D., Wang Y., Zhao T., and Bian R. L., (2004). Effect of Eucalyptus globulus oil on lipopolysaccharide induced chronic bronchitis and mucin hyper secretion in rats. Zhongguo Zhong yao za zhi, 29: 168–171.
[23]	Gilani A. H., and Atta-ur-Rahman J., (2005). Trends in ethnopharmacology. Journal of Ethnopharmacology, 100: 43-49.
[24]	Abad M. J., Ansuategui M., and Bermejo P., (2007). Active antifungal substances from natural sources. ARKIVOC, 8: 116-145.
[25]	Lawn S. D., and Zumla A. I., (2011). Tuberculosis. Lancet, 378: 57–72.
[26]	Zongo F., Ribout C., Boumendiel A., and Guissou I., (2013). Botany, traditional uses, phytochemistry and pharmacology of Waltheria indica L. (syn. Waltheria americana): A review Journal of Ethnopharmacology. 148: 14-26.
[27]	Ayantunde A. A., Hiernaux P., Briejer M., Udo H., and Tabo R., (2009). Uses of local plant species by Agro-pastoralists in South-western Niger. Ethnobotany Research and Applications, 7: 53-66.
[28]	Olowokudejo J. D., Kadiri A. B., Travih V. A., (2008). An ethnobotanical survey of herbal markets and medicinal plants in Lagos. Ethnobotanical Leaflets, 12: 851-865.
[29]	Phongpaichit S., Vuddhakul V., Subhadhirasaku S., and Wattanapiromsakul C., (2006). Evaluation of the anti-mycobacterial activity of extracts from plants used as self-medication by AIDS patients in Thailand. Journal of Pharmaceutical Biology, 44: 71-75.
[30]	Purushotham K. G., Arun P., Johnsy J. J., and Vasantha K. R., (2010). Screening of four Indian medicinal plants for in Vitro anti-mycobacterial activity. The Bioscan, 5: 103.
[31]	Oloye O. I., (2005). Chemical profile of Unripe pulp of Carica papaya pak. Journal of Nutrition, 4: 379-381.
[32]	Trease G., and Evans W., (2002). A textbook of pharmacognosy (fifth edition). E. Elsevier ltd. Edinburgh, 20-23.
[33]	Okwute S. K., and Isyaka M. S., (2014). Betulinic Acid from Antimicrobial Root Wood Extract of Dalbergia saxatilis Hook f. (Fabaceae). European Journal of Medicinal Plants 4: 686-694.
[34]	Cheesbrough M., (2002). Reaction Isolates on Tropical Diseases: The Effects. Cambridge University press, London, 2: 76-100.
[35]	European Committee on Antimicrobial Susceptibility Testing (EUCAST), (2012) Version 2: 12.
[36]	Suffredini I. B., Sander H. S., Goncalves A. G., Reis A. O., Gales A. C., Varella A. D., and Younes R. N., (2004). Screening of anti-bacterial extracts from plants native to Brazilian Amazon Rain Forest and Atlantic forest. Brazilian journal of Medical and Biological research, 37: 379-384.
[37]	Maryam J., Bushra M., Abida Y., and Mir A. K., (2012). Pharmacological activities of selected plant species and their phytochemical analysis. Journal of Medicinal Plants Research, 6: 5013-5022.
[38]	Choedon T., Mathan G., Arya S., Kumar V. L., and Kumar V., (2006). Anticancer and cytotoxic properties of the latex of Calotropis procera in a transgenic mouse model of hepatocellular carcinoma. World Journal of Gastroenterology, 12: 2517–2522.
[39]	Newman R. A., Yang P., Hittelman W. N., Lu T., Ho D. H., Ni D., Chan D., Vijeswarapu M., Cartwright C., Dixon S., Felix E., and Addington C., (2006). Oleandrin-mediated oxidative stress in human melanoma cells. Journal of Experimental Therapeutics and Oncology, 5: 167–181. Adesina S. K., Idowu O., Ogundaini A. O., Oladimeji H., Olugbade T. A., Onawunmi G. A., and Pais M., (2000). Antimicrobial constituents of the leaves of Acalypha wilkesiana and Acalypha hispida. Phytotheraphy Research, 14: 371-374.
[40]	Thomas G., (2007). Medicinal chemistry: An Introduction. 2nd edition: Wiley, England.
[41]	Hassan M. A., Oyewale A. O., Amupitan J. O., Abdullahi M. S., and Okonwo E. M., (2004). Preliminary phytochemical and antimicrobial investigation of crude extract of root bark of Deteriummi crocarpum. Nigerian Journal of Chemical Science, 29: 36-49.
[42]	Ngwu N. W., Effa E. B., Ftepti B. J., Gali A. I., Useh M. U., and Samuel C. J., (2016). Biochemical Studies of Ocimum sanctum and Olax subscorpioidea Leaf Extracts. British Journal of Pharmaceutical Research, 12: 1-9.
[43]	Kishore, N., Mishra B. B., Tripathi V., and Tiwari V. K., (2009). Alkaloids as potential anti-tubercular agents. Fitoterapia, 80: 149-163.
[44]	Lim K., Sim K., Tan G., and Kama T., (2009). Four tetracyclic oxindole alkaloids and a taberpsychine derivative from a Malayan Tabernaemontana. Phytochemistry, 70: 1182-1186.
[45]	Nurk E., Refsum H., Drevon C. A., Tell G. S., Nygaard H. A., Engedal K., and Smith A. D., (2009). Intake of flavonoid-rich wine, tea, and chocolate by elderly men and women is associated with better cognitive test performance. Journal of Nutrition, 139: 120–127.
[46]	Cazarolli L. H., Zanatta L., Alberton E. H., Figueiredo M. S., Folador P., Damazio R. G., Pizzolatti M. G., and Silva F. R., (2008). Flavonoids: Prospective Drug Candidates. Mini-Reviews in Medicinal Chemistry, 8: 1429–1440.
[47]	Cushnie T. P., and Lamb A. J., (2011). Recent advances in understanding the antibacterial properties of flavonoids. International Journal of Antimicrobial Agents, 38: 99–107.
[48]	Omojate G. C., Enwa F. O., Jewo A. O., Eze C. O., (2014). Mechanisms of Antimicrobial Actions of Phytochemicals against Enteric Pathogens – A Review. Journal of Pharmaceutical, Chemical and Biological Sciences, 2: 77-85.
[49]	Mabhiza D., Chitemerere T., and Mukanganyama S., (2016). Antibacterial Properties of Alkaloid Extracts from Callistemon citrinus and Vernonia adoensis against Staphylococcus aureus and Pseudomonas aeruginosa. International Journal of Medicinal Chemistry, 1: 1-7.
[50]	Dahiya P., and Purkayastha S., (2012). Phytochemical screening and antimicrobial activity of some medicinal plants against multi-drug resistant bacteria from clinical isolates. Indian Journal of Pharmaceutical Science, 74: 443–450.

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Okwute Simon Koma, Olakunle Ayodeji Fatokun, Orishadipe Abayomi Theophilus. (2017). Phytochemical Screening and in Vitro Antimicrobial Activity of Waltheria Indica Linn Leaf Extracts. Biomedical Sciences, 3(5), 86-93. https://doi.org/10.11648/j.bs.20170305.11

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ACS Style

Okwute Simon Koma; Olakunle Ayodeji Fatokun; Orishadipe Abayomi Theophilus. Phytochemical Screening and in Vitro Antimicrobial Activity of Waltheria Indica Linn Leaf Extracts. Biomed. Sci. 2017, 3(5), 86-93. doi: 10.11648/j.bs.20170305.11

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AMA Style

Okwute Simon Koma, Olakunle Ayodeji Fatokun, Orishadipe Abayomi Theophilus. Phytochemical Screening and in Vitro Antimicrobial Activity of Waltheria Indica Linn Leaf Extracts. Biomed Sci. 2017;3(5):86-93. doi: 10.11648/j.bs.20170305.11

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@article{10.11648/j.bs.20170305.11,
  author = {Okwute Simon Koma and Olakunle Ayodeji Fatokun and Orishadipe Abayomi Theophilus},
  title = {Phytochemical Screening and in Vitro Antimicrobial Activity of Waltheria Indica Linn Leaf Extracts},
  journal = {Biomedical Sciences},
  volume = {3},
  number = {5},
  pages = {86-93},
  doi = {10.11648/j.bs.20170305.11},
  url = {https://doi.org/10.11648/j.bs.20170305.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.bs.20170305.11},
  abstract = {The plant, Waltherica indica Linn is known to possess medicinal properties according to African folklore. Scientific verification of its bioactive constituents backing its use for the treatment of upper respiratory tract infections is limited. This study aimed at evaluating the phytochemical compounds present in the crude leaf extracts as well as identify their pathogen-specific antagonistic activities. The results obtained revealed that alkaloids, cardiac glycosides, phenolic acids, saponins, steroids, tannins and terpenoids were present in the extracts. The results also revealed that the zones of inhibition from the acidic, basic, neutral polar and non-polar fractions ranged between 24-28mm against the organisms  (Staphylococcus aureus, Streptococcus pneumonia, Streptococcus pyrogens, Klebsciella pneumonia, and Candida krusei). The Minimal Inhibitory Concentration (MICs) of the crude, acidic, basic, neutral polar and non-polar fractions of the leaf was recorded at 2.5mg/ml and 0.5mg/ml respectively. The results obtained suggested that the leaf of the studied plant possess strong antimicrobial activities against selected bacterial and fungal species with direct correlation to the actions of their phyto-constituents.},
 year = {2017}
}

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TY  - JOUR
T1  - Phytochemical Screening and in Vitro Antimicrobial Activity of Waltheria Indica Linn Leaf Extracts
AU  - Okwute Simon Koma
AU  - Olakunle Ayodeji Fatokun
AU  - Orishadipe Abayomi Theophilus
Y1  - 2017/10/06
PY  - 2017
N1  - https://doi.org/10.11648/j.bs.20170305.11
DO  - 10.11648/j.bs.20170305.11
T2  - Biomedical Sciences
JF  - Biomedical Sciences
JO  - Biomedical Sciences
SP  - 86
EP  - 93
PB  - Science Publishing Group
SN  - 2575-3932
UR  - https://doi.org/10.11648/j.bs.20170305.11
AB  - The plant, Waltherica indica Linn is known to possess medicinal properties according to African folklore. Scientific verification of its bioactive constituents backing its use for the treatment of upper respiratory tract infections is limited. This study aimed at evaluating the phytochemical compounds present in the crude leaf extracts as well as identify their pathogen-specific antagonistic activities. The results obtained revealed that alkaloids, cardiac glycosides, phenolic acids, saponins, steroids, tannins and terpenoids were present in the extracts. The results also revealed that the zones of inhibition from the acidic, basic, neutral polar and non-polar fractions ranged between 24-28mm against the organisms  (Staphylococcus aureus, Streptococcus pneumonia, Streptococcus pyrogens, Klebsciella pneumonia, and Candida krusei). The Minimal Inhibitory Concentration (MICs) of the crude, acidic, basic, neutral polar and non-polar fractions of the leaf was recorded at 2.5mg/ml and 0.5mg/ml respectively. The results obtained suggested that the leaf of the studied plant possess strong antimicrobial activities against selected bacterial and fungal species with direct correlation to the actions of their phyto-constituents.
VL  - 3
IS  - 5
ER  -

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Author Information

Okwute Simon Koma

Department of Chemistry, University of Abuja, Abuja, Nigeria
Olakunle Ayodeji Fatokun

Department of Chemistry, University of Abuja, Abuja, Nigeria
Orishadipe Abayomi Theophilus

Chemistry Advanced Research Centre, Sheda Science and Technology Complex (SHESTCO), Abuja, Nigeria

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APA Style

Okwute Simon Koma, Olakunle Ayodeji Fatokun, Orishadipe Abayomi Theophilus. (2017). Phytochemical Screening and in Vitro Antimicrobial Activity of Waltheria Indica Linn Leaf Extracts. Biomedical Sciences, 3(5), 86-93. https://doi.org/10.11648/j.bs.20170305.11

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ACS Style

Okwute Simon Koma; Olakunle Ayodeji Fatokun; Orishadipe Abayomi Theophilus. Phytochemical Screening and in Vitro Antimicrobial Activity of Waltheria Indica Linn Leaf Extracts. Biomed. Sci. 2017, 3(5), 86-93. doi: 10.11648/j.bs.20170305.11

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AMA Style

Okwute Simon Koma, Olakunle Ayodeji Fatokun, Orishadipe Abayomi Theophilus. Phytochemical Screening and in Vitro Antimicrobial Activity of Waltheria Indica Linn Leaf Extracts. Biomed Sci. 2017;3(5):86-93. doi: 10.11648/j.bs.20170305.11

Copy | Download

@article{10.11648/j.bs.20170305.11,
  author = {Okwute Simon Koma and Olakunle Ayodeji Fatokun and Orishadipe Abayomi Theophilus},
  title = {Phytochemical Screening and in Vitro Antimicrobial Activity of Waltheria Indica Linn Leaf Extracts},
  journal = {Biomedical Sciences},
  volume = {3},
  number = {5},
  pages = {86-93},
  doi = {10.11648/j.bs.20170305.11},
  url = {https://doi.org/10.11648/j.bs.20170305.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.bs.20170305.11},
  abstract = {The plant, Waltherica indica Linn is known to possess medicinal properties according to African folklore. Scientific verification of its bioactive constituents backing its use for the treatment of upper respiratory tract infections is limited. This study aimed at evaluating the phytochemical compounds present in the crude leaf extracts as well as identify their pathogen-specific antagonistic activities. The results obtained revealed that alkaloids, cardiac glycosides, phenolic acids, saponins, steroids, tannins and terpenoids were present in the extracts. The results also revealed that the zones of inhibition from the acidic, basic, neutral polar and non-polar fractions ranged between 24-28mm against the organisms  (Staphylococcus aureus, Streptococcus pneumonia, Streptococcus pyrogens, Klebsciella pneumonia, and Candida krusei). The Minimal Inhibitory Concentration (MICs) of the crude, acidic, basic, neutral polar and non-polar fractions of the leaf was recorded at 2.5mg/ml and 0.5mg/ml respectively. The results obtained suggested that the leaf of the studied plant possess strong antimicrobial activities against selected bacterial and fungal species with direct correlation to the actions of their phyto-constituents.},
 year = {2017}
}

Copy | Download

TY  - JOUR
T1  - Phytochemical Screening and in Vitro Antimicrobial Activity of Waltheria Indica Linn Leaf Extracts
AU  - Okwute Simon Koma
AU  - Olakunle Ayodeji Fatokun
AU  - Orishadipe Abayomi Theophilus
Y1  - 2017/10/06
PY  - 2017
N1  - https://doi.org/10.11648/j.bs.20170305.11
DO  - 10.11648/j.bs.20170305.11
T2  - Biomedical Sciences
JF  - Biomedical Sciences
JO  - Biomedical Sciences
SP  - 86
EP  - 93
PB  - Science Publishing Group
SN  - 2575-3932
UR  - https://doi.org/10.11648/j.bs.20170305.11
AB  - The plant, Waltherica indica Linn is known to possess medicinal properties according to African folklore. Scientific verification of its bioactive constituents backing its use for the treatment of upper respiratory tract infections is limited. This study aimed at evaluating the phytochemical compounds present in the crude leaf extracts as well as identify their pathogen-specific antagonistic activities. The results obtained revealed that alkaloids, cardiac glycosides, phenolic acids, saponins, steroids, tannins and terpenoids were present in the extracts. The results also revealed that the zones of inhibition from the acidic, basic, neutral polar and non-polar fractions ranged between 24-28mm against the organisms  (Staphylococcus aureus, Streptococcus pneumonia, Streptococcus pyrogens, Klebsciella pneumonia, and Candida krusei). The Minimal Inhibitory Concentration (MICs) of the crude, acidic, basic, neutral polar and non-polar fractions of the leaf was recorded at 2.5mg/ml and 0.5mg/ml respectively. The results obtained suggested that the leaf of the studied plant possess strong antimicrobial activities against selected bacterial and fungal species with direct correlation to the actions of their phyto-constituents.
VL  - 3
IS  - 5
ER  -

Copy | Download