Osteoarthritis (OA) is characterized by the degeneration of affected joints leading to pain and reduced mobility. Periostin appears as a potential candidate in transducing mechanical signals to stimulate chondrocyte proliferation. 15 rats (E-group) underwent both medial meniscectomy and medial collateral ligament (MCL) transaction, while the other 15 rats (S-group) underwent sham surgery. Animals were killed at 1, 2, and 4 weeks postsurgery, and 5 animals were put into use per-time point in each treatment group. Cartilage was harvested from tibial plateau and femoral condyle. We examined the level of Postn expression in cartilage of the experimental OA, using real-time PCR and immunohistochemistry analysis. The expression of Postn was about 2-fold higher at 1 week (P=0.08<0.05), 4-fold higher at 2 weeks (P=0.003<0.05) and 4-fold higher at 4 weeks (P=0.034<0.05) post-surgery in E-group compared to S-group. Postn protein expression levels were markedly increased in the E-Group compared with the S-Group at 1, 2 and 4 weeks post-surgery. This study will be of benefit to those subsequent studies using the OA model to evaluate the outcomes of Postn epxpression in mechanism of OA. Postn may play prominent roles in pathogenic mechanisms of OA.
| Published in | Biomedical Sciences (Volume 3, Issue 6) |
| DOI | 10.11648/j.bs.20170306.12 |
| Page(s) | 114-118 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2017. Published by Science Publishing Group |
Osteoarthritis (OA), Periostin (Postn), Cartilage, Rat Model of Osteoarthritis
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APA Style
Rongkai Zhang, Guowei Li, Dong Jiang, Lukun Yang, Dawei Zhang, et al. (2017). Elevated Expression of Periostin in Cartilage in Early Experimental Osteoarthritis. Biomedical Sciences, 3(6), 114-118. https://doi.org/10.11648/j.bs.20170306.12
ACS Style
Rongkai Zhang; Guowei Li; Dong Jiang; Lukun Yang; Dawei Zhang, et al. Elevated Expression of Periostin in Cartilage in Early Experimental Osteoarthritis. Biomed. Sci. 2017, 3(6), 114-118. doi: 10.11648/j.bs.20170306.12
AMA Style
Rongkai Zhang, Guowei Li, Dong Jiang, Lukun Yang, Dawei Zhang, et al. Elevated Expression of Periostin in Cartilage in Early Experimental Osteoarthritis. Biomed Sci. 2017;3(6):114-118. doi: 10.11648/j.bs.20170306.12
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Download@article{10.11648/j.bs.20170306.12,
author = {Rongkai Zhang and Guowei Li and Dong Jiang and Lukun Yang and Dawei Zhang and Zhe Wang and Jun Liang},
title = {Elevated Expression of Periostin in Cartilage in Early Experimental Osteoarthritis},
journal = {Biomedical Sciences},
volume = {3},
number = {6},
pages = {114-118},
doi = {10.11648/j.bs.20170306.12},
url = {https://doi.org/10.11648/j.bs.20170306.12},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.bs.20170306.12},
abstract = {Osteoarthritis (OA) is characterized by the degeneration of affected joints leading to pain and reduced mobility. Periostin appears as a potential candidate in transducing mechanical signals to stimulate chondrocyte proliferation. 15 rats (E-group) underwent both medial meniscectomy and medial collateral ligament (MCL) transaction, while the other 15 rats (S-group) underwent sham surgery. Animals were killed at 1, 2, and 4 weeks postsurgery, and 5 animals were put into use per-time point in each treatment group. Cartilage was harvested from tibial plateau and femoral condyle. We examined the level of Postn expression in cartilage of the experimental OA, using real-time PCR and immunohistochemistry analysis. The expression of Postn was about 2-fold higher at 1 week (P=0.08<0.05), 4-fold higher at 2 weeks (P=0.003<0.05) and 4-fold higher at 4 weeks (P=0.034<0.05) post-surgery in E-group compared to S-group. Postn protein expression levels were markedly increased in the E-Group compared with the S-Group at 1, 2 and 4 weeks post-surgery. This study will be of benefit to those subsequent studies using the OA model to evaluate the outcomes of Postn epxpression in mechanism of OA. Postn may play prominent roles in pathogenic mechanisms of OA.},
year = {2017}
}
TY - JOUR T1 - Elevated Expression of Periostin in Cartilage in Early Experimental Osteoarthritis AU - Rongkai Zhang AU - Guowei Li AU - Dong Jiang AU - Lukun Yang AU - Dawei Zhang AU - Zhe Wang AU - Jun Liang Y1 - 2017/12/05 PY - 2017 N1 - https://doi.org/10.11648/j.bs.20170306.12 DO - 10.11648/j.bs.20170306.12 T2 - Biomedical Sciences JF - Biomedical Sciences JO - Biomedical Sciences SP - 114 EP - 118 PB - Science Publishing Group SN - 2575-3932 UR - https://doi.org/10.11648/j.bs.20170306.12 AB - Osteoarthritis (OA) is characterized by the degeneration of affected joints leading to pain and reduced mobility. Periostin appears as a potential candidate in transducing mechanical signals to stimulate chondrocyte proliferation. 15 rats (E-group) underwent both medial meniscectomy and medial collateral ligament (MCL) transaction, while the other 15 rats (S-group) underwent sham surgery. Animals were killed at 1, 2, and 4 weeks postsurgery, and 5 animals were put into use per-time point in each treatment group. Cartilage was harvested from tibial plateau and femoral condyle. We examined the level of Postn expression in cartilage of the experimental OA, using real-time PCR and immunohistochemistry analysis. The expression of Postn was about 2-fold higher at 1 week (P=0.08<0.05), 4-fold higher at 2 weeks (P=0.003<0.05) and 4-fold higher at 4 weeks (P=0.034<0.05) post-surgery in E-group compared to S-group. Postn protein expression levels were markedly increased in the E-Group compared with the S-Group at 1, 2 and 4 weeks post-surgery. This study will be of benefit to those subsequent studies using the OA model to evaluate the outcomes of Postn epxpression in mechanism of OA. Postn may play prominent roles in pathogenic mechanisms of OA. VL - 3 IS - 6 ER -
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