Assessment of Malaria Microscopic Diagnosis Performance of Laboratory Professionals in Addis Ababa’s Public Health Facilities

Leykun Demeke Gebrekidan; Honelgn Nahusenay Hiruy

doi:doi:10.11648/j.bs.20190501.11

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Assessment of Malaria Microscopic Diagnosis Performance of Laboratory Professionals in Addis Ababa’s Public Health Facilities

Leykun Demeke Gebrekidan, Honelgn Nahusenay Hiruy

Published in Biomedical Sciences (Volume 5, Issue 1)

Received: 28 November 2018 Accepted: 5 January 2019 Published: 31 January 2019

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Abstract

Cross-sectional study was conducted using panel blood films and questioner to assess detection & identification performance of laboratory professionals’ and identify factors affecting the performance of malaria microscopic diagnosis. Study participants had 91.7% (95% CI: 89.96 – 93.44) agreement for detection of malaria parasites, 67.63% (95% CI: 64.91 – 70.35) species identification agreement for Plasmodium falciparum, 5.08% false positive and 21.04% false negative results. Correct species identification percentage for Plasmodium falciparum were 60.9% (510), Plasmodium vivax 59.17% (371) and Mixed (Plasmodium falciparum and Plasmodium vivax) 25% (53) were also identified in the study. In addition, sensitivity 94.69% (95% CI: 93.02 – 96.36) and specificity of 79.71 (95% CI: 75.22 – 84.2) were calculated from panel blood film results. The most frequent type of misdiagnosis was 85(40.09%) mixed BFs diagnosed as Plasmodium vivax, 67 (31.6%) mixed BFs as Plasmodium falciparum and 218(26%) Plasmodium falciparum BFs as Plasmodium vivax. Moreover, only 18(8.5%) laboratory professionals were participated in external quality assessment. From multiple logistic regression analysis training was the major factor for species identification percent agreement performance improvement of laboratory professionals. It showed statistical significance with p-value < 0.05 and untrained laboratory professionals were 64% less likely to perform ≥ 85% agreement of species identification. Training of laboratory professionals on malaria microscopic diagnosis help to improve the accuracy and reliability of reported results. This will help to provide the right and recommended medication and patient management.

Published in	Biomedical Sciences (Volume 5, Issue 1)
DOI	10.11648/j.bs.20190501.11
Page(s)	1-6
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2019. Published by Science Publishing Group

Keywords

Malaria, Microscopy, Diagnosis, Performance, Laboratory Professionals, Addis Ababa & Public Health Facilities

References

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APA Style

Leykun Demeke Gebrekidan, Honelgn Nahusenay Hiruy. (2019). Assessment of Malaria Microscopic Diagnosis Performance of Laboratory Professionals in Addis Ababa’s Public Health Facilities. Biomedical Sciences, 5(1), 1-6. https://doi.org/10.11648/j.bs.20190501.11

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ACS Style

Leykun Demeke Gebrekidan; Honelgn Nahusenay Hiruy. Assessment of Malaria Microscopic Diagnosis Performance of Laboratory Professionals in Addis Ababa’s Public Health Facilities. Biomed. Sci. 2019, 5(1), 1-6. doi: 10.11648/j.bs.20190501.11

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AMA Style

Leykun Demeke Gebrekidan, Honelgn Nahusenay Hiruy. Assessment of Malaria Microscopic Diagnosis Performance of Laboratory Professionals in Addis Ababa’s Public Health Facilities. Biomed Sci. 2019;5(1):1-6. doi: 10.11648/j.bs.20190501.11

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@article{10.11648/j.bs.20190501.11,
  author = {Leykun Demeke Gebrekidan and Honelgn Nahusenay Hiruy},
  title = {Assessment of Malaria Microscopic Diagnosis Performance of Laboratory Professionals in Addis Ababa’s Public Health Facilities},
  journal = {Biomedical Sciences},
  volume = {5},
  number = {1},
  pages = {1-6},
  doi = {10.11648/j.bs.20190501.11},
  url = {https://doi.org/10.11648/j.bs.20190501.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.bs.20190501.11},
  abstract = {Cross-sectional study was conducted using panel blood films and questioner to assess detection & identification performance of laboratory professionals’ and identify factors affecting the performance of malaria microscopic diagnosis. Study participants had 91.7% (95% CI: 89.96 – 93.44) agreement for detection of malaria parasites, 67.63% (95% CI: 64.91 – 70.35) species identification agreement for Plasmodium falciparum, 5.08% false positive and 21.04% false negative results. Correct species identification percentage for Plasmodium falciparum were 60.9% (510), Plasmodium vivax 59.17% (371) and Mixed (Plasmodium falciparum and Plasmodium vivax) 25% (53) were also identified in the study. In addition, sensitivity 94.69% (95% CI: 93.02 – 96.36) and specificity of 79.71 (95% CI: 75.22 – 84.2) were calculated from panel blood film results. The most frequent type of misdiagnosis was 85(40.09%) mixed BFs diagnosed as Plasmodium vivax, 67 (31.6%) mixed BFs as Plasmodium falciparum and 218(26%) Plasmodium falciparum BFs as Plasmodium vivax. Moreover, only 18(8.5%) laboratory professionals were participated in external quality assessment. From multiple logistic regression analysis training was the major factor for species identification percent agreement performance improvement of laboratory professionals. It showed statistical significance with p-value < 0.05 and untrained laboratory professionals were 64% less likely to perform ≥ 85% agreement of species identification. Training of laboratory professionals on malaria microscopic diagnosis help to improve the accuracy and reliability of reported results. This will help to provide the right and recommended medication and patient management.},
 year = {2019}
}

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TY  - JOUR
T1  - Assessment of Malaria Microscopic Diagnosis Performance of Laboratory Professionals in Addis Ababa’s Public Health Facilities
AU  - Leykun Demeke Gebrekidan
AU  - Honelgn Nahusenay Hiruy
Y1  - 2019/01/31
PY  - 2019
N1  - https://doi.org/10.11648/j.bs.20190501.11
DO  - 10.11648/j.bs.20190501.11
T2  - Biomedical Sciences
JF  - Biomedical Sciences
JO  - Biomedical Sciences
SP  - 1
EP  - 6
PB  - Science Publishing Group
SN  - 2575-3932
UR  - https://doi.org/10.11648/j.bs.20190501.11
AB  - Cross-sectional study was conducted using panel blood films and questioner to assess detection & identification performance of laboratory professionals’ and identify factors affecting the performance of malaria microscopic diagnosis. Study participants had 91.7% (95% CI: 89.96 – 93.44) agreement for detection of malaria parasites, 67.63% (95% CI: 64.91 – 70.35) species identification agreement for Plasmodium falciparum, 5.08% false positive and 21.04% false negative results. Correct species identification percentage for Plasmodium falciparum were 60.9% (510), Plasmodium vivax 59.17% (371) and Mixed (Plasmodium falciparum and Plasmodium vivax) 25% (53) were also identified in the study. In addition, sensitivity 94.69% (95% CI: 93.02 – 96.36) and specificity of 79.71 (95% CI: 75.22 – 84.2) were calculated from panel blood film results. The most frequent type of misdiagnosis was 85(40.09%) mixed BFs diagnosed as Plasmodium vivax, 67 (31.6%) mixed BFs as Plasmodium falciparum and 218(26%) Plasmodium falciparum BFs as Plasmodium vivax. Moreover, only 18(8.5%) laboratory professionals were participated in external quality assessment. From multiple logistic regression analysis training was the major factor for species identification percent agreement performance improvement of laboratory professionals. It showed statistical significance with p-value < 0.05 and untrained laboratory professionals were 64% less likely to perform ≥ 85% agreement of species identification. Training of laboratory professionals on malaria microscopic diagnosis help to improve the accuracy and reliability of reported results. This will help to provide the right and recommended medication and patient management.
VL  - 5
IS  - 1
ER  -

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Author Information

Leykun Demeke Gebrekidan

ICAP at Columbia University, Addis Ababa, Ethiopia
Honelgn Nahusenay Hiruy

Addis Continental Institute of Public Health, Addis Ababa, Ethiopia

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APA Style

Leykun Demeke Gebrekidan, Honelgn Nahusenay Hiruy. (2019). Assessment of Malaria Microscopic Diagnosis Performance of Laboratory Professionals in Addis Ababa’s Public Health Facilities. Biomedical Sciences, 5(1), 1-6. https://doi.org/10.11648/j.bs.20190501.11

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ACS Style

Leykun Demeke Gebrekidan; Honelgn Nahusenay Hiruy. Assessment of Malaria Microscopic Diagnosis Performance of Laboratory Professionals in Addis Ababa’s Public Health Facilities. Biomed. Sci. 2019, 5(1), 1-6. doi: 10.11648/j.bs.20190501.11

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AMA Style

Leykun Demeke Gebrekidan, Honelgn Nahusenay Hiruy. Assessment of Malaria Microscopic Diagnosis Performance of Laboratory Professionals in Addis Ababa’s Public Health Facilities. Biomed Sci. 2019;5(1):1-6. doi: 10.11648/j.bs.20190501.11

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@article{10.11648/j.bs.20190501.11,
  author = {Leykun Demeke Gebrekidan and Honelgn Nahusenay Hiruy},
  title = {Assessment of Malaria Microscopic Diagnosis Performance of Laboratory Professionals in Addis Ababa’s Public Health Facilities},
  journal = {Biomedical Sciences},
  volume = {5},
  number = {1},
  pages = {1-6},
  doi = {10.11648/j.bs.20190501.11},
  url = {https://doi.org/10.11648/j.bs.20190501.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.bs.20190501.11},
  abstract = {Cross-sectional study was conducted using panel blood films and questioner to assess detection & identification performance of laboratory professionals’ and identify factors affecting the performance of malaria microscopic diagnosis. Study participants had 91.7% (95% CI: 89.96 – 93.44) agreement for detection of malaria parasites, 67.63% (95% CI: 64.91 – 70.35) species identification agreement for Plasmodium falciparum, 5.08% false positive and 21.04% false negative results. Correct species identification percentage for Plasmodium falciparum were 60.9% (510), Plasmodium vivax 59.17% (371) and Mixed (Plasmodium falciparum and Plasmodium vivax) 25% (53) were also identified in the study. In addition, sensitivity 94.69% (95% CI: 93.02 – 96.36) and specificity of 79.71 (95% CI: 75.22 – 84.2) were calculated from panel blood film results. The most frequent type of misdiagnosis was 85(40.09%) mixed BFs diagnosed as Plasmodium vivax, 67 (31.6%) mixed BFs as Plasmodium falciparum and 218(26%) Plasmodium falciparum BFs as Plasmodium vivax. Moreover, only 18(8.5%) laboratory professionals were participated in external quality assessment. From multiple logistic regression analysis training was the major factor for species identification percent agreement performance improvement of laboratory professionals. It showed statistical significance with p-value < 0.05 and untrained laboratory professionals were 64% less likely to perform ≥ 85% agreement of species identification. Training of laboratory professionals on malaria microscopic diagnosis help to improve the accuracy and reliability of reported results. This will help to provide the right and recommended medication and patient management.},
 year = {2019}
}

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TY  - JOUR
T1  - Assessment of Malaria Microscopic Diagnosis Performance of Laboratory Professionals in Addis Ababa’s Public Health Facilities
AU  - Leykun Demeke Gebrekidan
AU  - Honelgn Nahusenay Hiruy
Y1  - 2019/01/31
PY  - 2019
N1  - https://doi.org/10.11648/j.bs.20190501.11
DO  - 10.11648/j.bs.20190501.11
T2  - Biomedical Sciences
JF  - Biomedical Sciences
JO  - Biomedical Sciences
SP  - 1
EP  - 6
PB  - Science Publishing Group
SN  - 2575-3932
UR  - https://doi.org/10.11648/j.bs.20190501.11
AB  - Cross-sectional study was conducted using panel blood films and questioner to assess detection & identification performance of laboratory professionals’ and identify factors affecting the performance of malaria microscopic diagnosis. Study participants had 91.7% (95% CI: 89.96 – 93.44) agreement for detection of malaria parasites, 67.63% (95% CI: 64.91 – 70.35) species identification agreement for Plasmodium falciparum, 5.08% false positive and 21.04% false negative results. Correct species identification percentage for Plasmodium falciparum were 60.9% (510), Plasmodium vivax 59.17% (371) and Mixed (Plasmodium falciparum and Plasmodium vivax) 25% (53) were also identified in the study. In addition, sensitivity 94.69% (95% CI: 93.02 – 96.36) and specificity of 79.71 (95% CI: 75.22 – 84.2) were calculated from panel blood film results. The most frequent type of misdiagnosis was 85(40.09%) mixed BFs diagnosed as Plasmodium vivax, 67 (31.6%) mixed BFs as Plasmodium falciparum and 218(26%) Plasmodium falciparum BFs as Plasmodium vivax. Moreover, only 18(8.5%) laboratory professionals were participated in external quality assessment. From multiple logistic regression analysis training was the major factor for species identification percent agreement performance improvement of laboratory professionals. It showed statistical significance with p-value < 0.05 and untrained laboratory professionals were 64% less likely to perform ≥ 85% agreement of species identification. Training of laboratory professionals on malaria microscopic diagnosis help to improve the accuracy and reliability of reported results. This will help to provide the right and recommended medication and patient management.
VL  - 5
IS  - 1
ER  -

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