Microbial Changes in the Rhizosphere of Paper Mulberry (<i>Broussonetia papyrifera</i>) Mutant with a High Concentration of Crude Protein

Zhaoxiang Wu; Qiaoli Liu; Yanqiang Li; Huihu Li; Yongda Zhong; Faxin Yu

doi:doi:10.11648/j.earth.20211001.12

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Microbial Changes in the Rhizosphere of Paper Mulberry (Broussonetia papyrifera) Mutant with a High Concentration of Crude Protein

Zhaoxiang Wu, Qiaoli Liu, Yanqiang Li, Huihu Li, Yongda Zhong, Faxin Yu

Published in Earth Sciences (Volume 10, Issue 1)

Received: 2 February 2021 Accepted: 18 February 2021 Published: 26 February 2021

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Abstract

Recently, many studies involving plant-microorganism relationships in the rhizosphere of multitudinous important economic crops revealed a clear signature of the host plant in shaping its rhizosphere microbial composition and structure. The nutrient preference of host plant was suggested to be one important factor determining the structure and assembly of the rhizosphere microbiome, but the proof for this hypothesis is still not enough. In this study, soil microbiomes in the rhizosphere of two Paper mulberry varieties with different nitrogen absorption and utilization efficiency were investigated using a short term pot experiment in controlled greenhouse, and the physicochemical properties were also determined. The results showed that, compared to the control plants, the mutated Paper mulberry variety with high N demand reduced the microbial growth significantly and changed the bacterial and the fungal composition in the rhizosphere soils, and alkaline nitrogen was identified to be the most significant factor affecting soil microbial community. Moreover, the effects of excessive consumption of soil nutrient during Paper mulberry cultivation on the microbiome was revealed, and it could be employed in field water and fertilizer management of Paper mulberry planting. This study further confirmed that the soil nutrient status resulting from the plant nutrient preference drives the development of a plant-specific microbiome.

Published in	Earth Sciences (Volume 10, Issue 1)
DOI	10.11648/j.earth.20211001.12
Page(s)	7-16
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2021. Published by Science Publishing Group

Keywords

Paper Mulberry, Nutrient Preference, MiSeq Sequencing, Rhizosphere, Soil Microbial Community

References

[1]	Chaparro, J. M., Badri, D. V., & Vivanco, J. M., 2013. Rhizosphere microbiome assemblage is affected by plant development. The ISME Journal 8, 790-803.
[2]	Brimecombe, M. J., De-Lelj, F. A., Lynch, J. M., The effect of root exudates on rhizosphere microbial populations. In: Pinton, R., Varanini, Z., Nannipieri, P. (eds) The rhizosphere. Biochemistry and organic substances at the soil-plant interface. Marcel Dekker, New York, 2001, 95-140.
[3]	Badri, D. V., Vivanco, J. M., 2009. Regulation and function of root exudates. Plant Cell Environment 32, 666-681.
[4]	Bakker, M. G., Manter, D. K., Sheflin, A. M., Weir, T. L., Vivanco, J. M., 2012. Harnessing the rhizosphere microbiome through plant breeding and agricultural management. Plant Soil 360, 1-13.
[5]	Fakhro, A., Andrade-Linares, D. R., von Bargen, S., Bandte, M., Büttner, C., Grosch, R., Schwarz, D., Franken, P., 2009. Impact of Piriformospora indica on tomato growth and on interaction with fungal and viral pathogens. Mycorrhiza, 20, 191-200.
[6]	Youssef, G. H., Seddik, W. M. A., Osman, M. A., 2010. Efficiency of natural minerals in presence of different nitrogen forms and potassium dissolving bacteria on peanut and sesame yields. American Journal of Science 6, 647-660.
[7]	Van der Meer, J. R., 1997. Evolution of novel metabolic pathways for the degradation of chloroaromatic compounds. Antonie van Leeuwenhoek 71, 159-178.
[8]	Li, T., Hu, Y. J., Hao, Z. P., Li, H., Wang, Y. S., & Chen, B. D., 2012. First cloning and characterization of two functional aquaporin genes from an arbuscular mycorrhizal fungus Glomus intraradices. New Phytologist 197, 617-630.
[9]	Berg, G., Grube, M., Schloter, M., Smalla, K., 2014. Unraveling the plant microbiome: looking back and future perspectives. Frontiers in Microbiology 5, 148.
[10]	Mueller, U. G., Sachs, J. L., 2015. Engineering microbiomes to improve plant and animal health. Trends Microbiology 23, 606–617.
[11]	Burns, J. H., Anacker, B. L., Strauss, S. Y., & Burke, D. J., 2015. Soil microbial community variation correlates most strongly with plant species identity, followed by soil chemistry, spatial location and plant genus. AoB PLANTS, 7, plv030-plv030.
[12]	Xun, W., Huang, T., Zhao, J., Ran, W., Wang, B., Shen, Q., Zhang, R., 2015. Environmental conditions rather than microbial inoculum composition determine the bacterial composition, microbial biomass and enzymatic activity of reconstructed soil microbial communities. Soil Biology and Biochemistry 90, 10-18.
[13]	Ofek, M., Voronov-Goldman, M., Hadar, Y., Minz, D., 2014. Host signature effect on plant root-associated microbiomes revealed through analyses of resident vs. active communities. Environmental Microbiology 16, 2157-2167.
[14]	Ai, C., Liang, G., Sun, J., Wang, X., He, P., Zhou, W., He, X., 2015. Reduced dependence of rhizosphere microbiome on plant-derived carbon in 32-year long-term inorganic and organic fertilized soils. Soil Biology and Biochemistry 80, 70-78.
[15]	Liao, S. X., Li, K., Yang, Z. Y., Zhang, C. J., 2006. Influence of age on chemical components, fiber morphology and pulping proper- ties of Broussonetia papyrifera bark. Forestry Research 19, 436-440.
[16]	Peng, X., Liu, H., Chen, P., Tang, F., Hu, Y., Wang, F., Pi, Z., Zhao, M., Chen, N., Chen, H., Zhang, X., Yan, X., Liu, M., Fu, X., Zhao, G., Yao, P., Wang, L., Dai, H., Li, X., Xiong, W., Xu, W., Zheng, H., Yu, H., and Shen, S., 2019. A Chromosome-Scale Genome Assembly of Paper Mulberry (Broussonetia papyrifera) Provides New Insights into Its Forage and Papermaking Usage. Molecular Plant. 12: 661–677.
[17]	Zhai, X., Zeng, H., Liu, Y., and Liu, F., 2012. Change of nutrients and shape of Broussonetia papyrifera leaves from different clones. Journal of Northeast Forestry University 40, 38-52.
[18]	Huang, S., Ding, J., Deng, D., Tang, W., Sun, H., Liu, D., Zhang, L., Niu, X., Zhang, X., Meng, M., et al., 2013. Draft genome of the kiwifruit Actinidia chinensis. Nature Communications. 4, 1-9.
[19]	Shen, S., and Peng, X., 2017. The black tech in“Paper Mulberry Poverty Alleviation” fills the lack of crude protein feed material of Chinese animal husbandry. Science, Technology and Development 13, 435-442.
[20]	Lichtenthaler, H. K., 1987. Chlorophylls and carotenoids-pigments of photosynthetic biomembranes. Methods in Enzymology 148, 350-382.
[21]	Qiu, M., Zhang, R., Xue, C., Zhang, S., Li, S., Zhang, N., and Shen, Q., 2012. Application of bio-organic fertilizer can control Fusarium wilt of cucumber plants by regulating microbial community of rhizosphere soil. Biology and Fertility of Soils 48, 807-816.
[22]	Page, A. L., Miller, R. H., Keeney, D. R., 1982. Total carbon, organic carbon and organic matter. In: American Society of Agronomy (Ed.), Methods of Soil Analysis. Part 2, Agronomy. Madison, WI, pp 539-579.
[23]	Cornfield, A. H., 1960. Ammonia released on treating soils with N sodium hydroxide as a possible means of predicting the nitrogen-supplying power of soils. Nature 187, 260-261.
[24]	Gyaneshwar, P., Kumar, G. N., Parekh, L. J., and Poole, P. S., 2002. Role of soil microorganisms in improving P nutrition of plants. Food Security in Nutrient-Stressed Environments: Exploiting Plants’ Genetic Capabilities. Springer, Netherlands 133-143.
[25]	Bergmann, G. T., Bates, S. T., Eilers, K. G., Lauber, C. L., Caporaso, J. G., Walters, W. A., Knight, R., Fierer, N., 2011. The under-recognized dominance of Verrucomicrobia in soil bacterial communities. Soil Biology and Biochemistry 43, 1450-1455.
[26]	Rajala, T., Peltoniemi, M., Pennanen, T., and Mäkipää, R., 2012. Fungal community dynamics in relation to substrate quality of decaying Norway spruce (Picea abies [L.] Karst.) logs in boreal forests. FEMS Microbiology Ecology 81, 494-505.
[27]	Caporaso, J. G., Kuczynski, J., Stombaugh, J., Bittinger, K., Bushman, F. D., Costello, E. K., Fierer, N., Peña, A. G., Goodrich, J. K., Gordon, J. I., Huttley, G. A., Kelley, S. T., Knights, D., Koenig, J. E., Ley, R. E., Lozupone, C. A., McDonald, D., Muegge, B. D., Pirrung, M., Reeder, J., Sevinsky, J. R., Turnbaugh, P. J., Walters, W. A., Widmann, J., Yatsunenko, T., Zaneveld, J., Knight, R., 2010. QIIME allows analysis of high-throughput community sequencing data. Nature Methods 7, 335-336.
[28]	Pruesse, E., Quast, C., Knittel, K., Fuchs, B. M., Ludwig, W., Peplies, J., Glöckner, F. O., 2007. SILVA: a comprehensive online resource for quality checked and aligned ribosomal RNA sequence data compatible with ARB. Nucleic Acids Research 35, 7188-7196.
[29]	Hao, X., Jiang, R., Chen, T., 2011. Clustering 16S rRNA for OTU prediction: a method of unsupervised Bayesian clustering. Bioinformatics 27, 611-618.
[30]	Henrik-Nilsson, R., Tedersoo, L., Lindahl, B. D., Kjøller, R., Carlsen, T., Quince, C., Abarenkov, K., Pennanen, T., Stenlid, J., Bruns, T., Larsson, K. H., Kõljalg, U. and Kauserud, H., 2011. Towards standardization of the description and publication of next-generation sequencing datasets of fungal communities. New Phytologist 191, 314-318.
[31]	Abarenkov, K., Henrik-Nilsson, R., Larsson, K. H., Alexander, I. J., Eberhardt, U., Erland, S., Høiland, K., Kjøller, R., Larsson, E., Pennanen, T., Sen, R., Taylor, A. F., Tedersoo, L., Ursing, B. M., Vrälstad, T., Liimatainen, K., Peintner, U. and Kõljalg, U., 2010. The UNITE database for molecular identification of fungi-recent updates and future perspectives. New Phytologist 186, 281-285.
[32]	Oksanen, J., 2011. Multivariate analysis of ecological communities in R: vegan tutorial. R package version 1 (7).
[33]	Clarke, K. R., 1993. Non-parametric multivariate analysis of changes in community structure. Australian Journal of Ecology 18, 117-143.
[34]	Langille M. G. I., Zaneveld J., Caporaso J. G., et al., 2013. Predictive functional profiling of microbial communities using 16S rRNA marker gene sequences. Nature biotechnology 31, 814-821.
[35]	Hawkesford, M., Horst, W., Kichey, T., Lambers, H., Schjoerring, J., & Inge Skrumsager Møller, et al., 2012. Chapter 6 - functions of macronutrients. In Marschners Mineral Nutrition of Higher Plants (3rd ed), pp. 135-189. Academic Press, London.
[36]	Wang, Y. Y., Cheng, Y. H., Chen, K. E., et al., 2018. Nitrate transport, signaling, and use efficiency. Annual Review of Plant Biology 69, 85-122.
[37]	Wang, G. L., Ding, G. D., Li, L., Cai, H. M., Ye, X. S., Zou, J., & Xu, F. S., 2014. Identification and characterization of improved nitrogen efficiency in interspecific hybridized new-type Brassica napus. Annals of Botany 114, 549-559.
[38]	Bahram, M., Hildebrand, F., Forslund, S. K., Anderson, J. L., Soudzilovskaia, N. A., Bodegom, P. M., Bengtsson-Palme, J., Anslan, S., Coelho, L. P., Harend, H., HUERTA-Cepas, J., Medema, M. H., Maltz, M. R., Mundra, S., Olsson P. A., Pent, M., Põlme, S., Sunagawa, S., Ryberg, M., Tedersoo, L., Bork, P., 2018. Structure and function of the global topsoil microbiome. Nature 560 (7717), 233-237.
[39]	Olden, J. D., Poff, N. L., Douglas, M. R., Douglas, M. E., Fausch, K. D., 2004. Ecological and evolutionary consequences of biotic homogenization. Trends in Ecology and Evolution 19, 18-24.
[40]	Yu, Z., Peng, Y., Peng, Z., Chai, T., 2013. Influence of nitrogen shortage on activated sludge system. Journal of Harbin University of Commerce 29, 27-31.
[41]	Yuan, J., Chaparro, J. M., Manter, D. K., Zhang, R., Vivanco, J. M., Shen, Q., 2015. Roots from distinct plant developmental stages are capable of rapidly selecting their own microbiome without the influence of environmental and soil edaphic factors. Soil Biology and Biochemistry 89, 206-209.
[42]	Cai, F., Pang, G., Miao, Y., Li, R., Li, R., Shen, Q., & Chen, W., 2017. The nutrient preference of plants influences their rhizosphere microbiome. Applied Soil Ecology 110, 146-150.

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APA Style

Zhaoxiang Wu, Qiaoli Liu, Yanqiang Li, Huihu Li, Yongda Zhong, et al. (2021). Microbial Changes in the Rhizosphere of Paper Mulberry (Broussonetia papyrifera) Mutant with a High Concentration of Crude Protein. Earth Sciences, 10(1), 7-16. https://doi.org/10.11648/j.earth.20211001.12

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ACS Style

Zhaoxiang Wu; Qiaoli Liu; Yanqiang Li; Huihu Li; Yongda Zhong, et al. Microbial Changes in the Rhizosphere of Paper Mulberry (Broussonetia papyrifera) Mutant with a High Concentration of Crude Protein. Earth Sci. 2021, 10(1), 7-16. doi: 10.11648/j.earth.20211001.12

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AMA Style

Zhaoxiang Wu, Qiaoli Liu, Yanqiang Li, Huihu Li, Yongda Zhong, et al. Microbial Changes in the Rhizosphere of Paper Mulberry (Broussonetia papyrifera) Mutant with a High Concentration of Crude Protein. Earth Sci. 2021;10(1):7-16. doi: 10.11648/j.earth.20211001.12

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@article{10.11648/j.earth.20211001.12,
  author = {Zhaoxiang Wu and Qiaoli Liu and Yanqiang Li and Huihu Li and Yongda Zhong and Faxin Yu},
  title = {Microbial Changes in the Rhizosphere of Paper Mulberry (Broussonetia papyrifera) Mutant with a High Concentration of Crude Protein},
  journal = {Earth Sciences},
  volume = {10},
  number = {1},
  pages = {7-16},
  doi = {10.11648/j.earth.20211001.12},
  url = {https://doi.org/10.11648/j.earth.20211001.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.earth.20211001.12},
  abstract = {Recently, many studies involving plant-microorganism relationships in the rhizosphere of multitudinous important economic crops revealed a clear signature of the host plant in shaping its rhizosphere microbial composition and structure. The nutrient preference of host plant was suggested to be one important factor determining the structure and assembly of the rhizosphere microbiome, but the proof for this hypothesis is still not enough. In this study, soil microbiomes in the rhizosphere of two Paper mulberry varieties with different nitrogen absorption and utilization efficiency were investigated using a short term pot experiment in controlled greenhouse, and the physicochemical properties were also determined. The results showed that, compared to the control plants, the mutated Paper mulberry variety with high N demand reduced the microbial growth significantly and changed the bacterial and the fungal composition in the rhizosphere soils, and alkaline nitrogen was identified to be the most significant factor affecting soil microbial community. Moreover, the effects of excessive consumption of soil nutrient during Paper mulberry cultivation on the microbiome was revealed, and it could be employed in field water and fertilizer management of Paper mulberry planting. This study further confirmed that the soil nutrient status resulting from the plant nutrient preference drives the development of a plant-specific microbiome.},
 year = {2021}
}

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TY  - JOUR
T1  - Microbial Changes in the Rhizosphere of Paper Mulberry (Broussonetia papyrifera) Mutant with a High Concentration of Crude Protein
AU  - Zhaoxiang Wu
AU  - Qiaoli Liu
AU  - Yanqiang Li
AU  - Huihu Li
AU  - Yongda Zhong
AU  - Faxin Yu
Y1  - 2021/02/26
PY  - 2021
N1  - https://doi.org/10.11648/j.earth.20211001.12
DO  - 10.11648/j.earth.20211001.12
T2  - Earth Sciences
JF  - Earth Sciences
JO  - Earth Sciences
SP  - 7
EP  - 16
PB  - Science Publishing Group
SN  - 2328-5982
UR  - https://doi.org/10.11648/j.earth.20211001.12
AB  - Recently, many studies involving plant-microorganism relationships in the rhizosphere of multitudinous important economic crops revealed a clear signature of the host plant in shaping its rhizosphere microbial composition and structure. The nutrient preference of host plant was suggested to be one important factor determining the structure and assembly of the rhizosphere microbiome, but the proof for this hypothesis is still not enough. In this study, soil microbiomes in the rhizosphere of two Paper mulberry varieties with different nitrogen absorption and utilization efficiency were investigated using a short term pot experiment in controlled greenhouse, and the physicochemical properties were also determined. The results showed that, compared to the control plants, the mutated Paper mulberry variety with high N demand reduced the microbial growth significantly and changed the bacterial and the fungal composition in the rhizosphere soils, and alkaline nitrogen was identified to be the most significant factor affecting soil microbial community. Moreover, the effects of excessive consumption of soil nutrient during Paper mulberry cultivation on the microbiome was revealed, and it could be employed in field water and fertilizer management of Paper mulberry planting. This study further confirmed that the soil nutrient status resulting from the plant nutrient preference drives the development of a plant-specific microbiome.
VL  - 10
IS  - 1
ER  -

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Author Information

Zhaoxiang Wu

Institute of Biological Resources, Jiangxi Academy of Sciences, Nanchang, China
Qiaoli Liu

Institute of Biological Resources, Jiangxi Academy of Sciences, Nanchang, China
Yanqiang Li

Institute of Biological Resources, Jiangxi Academy of Sciences, Nanchang, China
Huihu Li

Institute of Biological Resources, Jiangxi Academy of Sciences, Nanchang, China
Yongda Zhong

Institute of Biological Resources, Jiangxi Academy of Sciences, Nanchang, China
Faxin Yu

Institute of Biological Resources, Jiangxi Academy of Sciences, Nanchang, China

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APA Style

Zhaoxiang Wu, Qiaoli Liu, Yanqiang Li, Huihu Li, Yongda Zhong, et al. (2021). Microbial Changes in the Rhizosphere of Paper Mulberry (Broussonetia papyrifera) Mutant with a High Concentration of Crude Protein. Earth Sciences, 10(1), 7-16. https://doi.org/10.11648/j.earth.20211001.12

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ACS Style

Zhaoxiang Wu; Qiaoli Liu; Yanqiang Li; Huihu Li; Yongda Zhong, et al. Microbial Changes in the Rhizosphere of Paper Mulberry (Broussonetia papyrifera) Mutant with a High Concentration of Crude Protein. Earth Sci. 2021, 10(1), 7-16. doi: 10.11648/j.earth.20211001.12

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AMA Style

Zhaoxiang Wu, Qiaoli Liu, Yanqiang Li, Huihu Li, Yongda Zhong, et al. Microbial Changes in the Rhizosphere of Paper Mulberry (Broussonetia papyrifera) Mutant with a High Concentration of Crude Protein. Earth Sci. 2021;10(1):7-16. doi: 10.11648/j.earth.20211001.12

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@article{10.11648/j.earth.20211001.12,
  author = {Zhaoxiang Wu and Qiaoli Liu and Yanqiang Li and Huihu Li and Yongda Zhong and Faxin Yu},
  title = {Microbial Changes in the Rhizosphere of Paper Mulberry (Broussonetia papyrifera) Mutant with a High Concentration of Crude Protein},
  journal = {Earth Sciences},
  volume = {10},
  number = {1},
  pages = {7-16},
  doi = {10.11648/j.earth.20211001.12},
  url = {https://doi.org/10.11648/j.earth.20211001.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.earth.20211001.12},
  abstract = {Recently, many studies involving plant-microorganism relationships in the rhizosphere of multitudinous important economic crops revealed a clear signature of the host plant in shaping its rhizosphere microbial composition and structure. The nutrient preference of host plant was suggested to be one important factor determining the structure and assembly of the rhizosphere microbiome, but the proof for this hypothesis is still not enough. In this study, soil microbiomes in the rhizosphere of two Paper mulberry varieties with different nitrogen absorption and utilization efficiency were investigated using a short term pot experiment in controlled greenhouse, and the physicochemical properties were also determined. The results showed that, compared to the control plants, the mutated Paper mulberry variety with high N demand reduced the microbial growth significantly and changed the bacterial and the fungal composition in the rhizosphere soils, and alkaline nitrogen was identified to be the most significant factor affecting soil microbial community. Moreover, the effects of excessive consumption of soil nutrient during Paper mulberry cultivation on the microbiome was revealed, and it could be employed in field water and fertilizer management of Paper mulberry planting. This study further confirmed that the soil nutrient status resulting from the plant nutrient preference drives the development of a plant-specific microbiome.},
 year = {2021}
}

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TY  - JOUR
T1  - Microbial Changes in the Rhizosphere of Paper Mulberry (Broussonetia papyrifera) Mutant with a High Concentration of Crude Protein
AU  - Zhaoxiang Wu
AU  - Qiaoli Liu
AU  - Yanqiang Li
AU  - Huihu Li
AU  - Yongda Zhong
AU  - Faxin Yu
Y1  - 2021/02/26
PY  - 2021
N1  - https://doi.org/10.11648/j.earth.20211001.12
DO  - 10.11648/j.earth.20211001.12
T2  - Earth Sciences
JF  - Earth Sciences
JO  - Earth Sciences
SP  - 7
EP  - 16
PB  - Science Publishing Group
SN  - 2328-5982
UR  - https://doi.org/10.11648/j.earth.20211001.12
AB  - Recently, many studies involving plant-microorganism relationships in the rhizosphere of multitudinous important economic crops revealed a clear signature of the host plant in shaping its rhizosphere microbial composition and structure. The nutrient preference of host plant was suggested to be one important factor determining the structure and assembly of the rhizosphere microbiome, but the proof for this hypothesis is still not enough. In this study, soil microbiomes in the rhizosphere of two Paper mulberry varieties with different nitrogen absorption and utilization efficiency were investigated using a short term pot experiment in controlled greenhouse, and the physicochemical properties were also determined. The results showed that, compared to the control plants, the mutated Paper mulberry variety with high N demand reduced the microbial growth significantly and changed the bacterial and the fungal composition in the rhizosphere soils, and alkaline nitrogen was identified to be the most significant factor affecting soil microbial community. Moreover, the effects of excessive consumption of soil nutrient during Paper mulberry cultivation on the microbiome was revealed, and it could be employed in field water and fertilizer management of Paper mulberry planting. This study further confirmed that the soil nutrient status resulting from the plant nutrient preference drives the development of a plant-specific microbiome.
VL  - 10
IS  - 1
ER  -

Copy | Download