Ampicillin Resistance in <i>Haemophilus influenzae</i> Isolated from Acute Respiratory Infections in Pediatrics

Abdoulaye Diop; Assane Dieng; Abdoulaye Seck; Amary Fall; Amadou Diop; Djibril Boiro; Jean Baptisse Niokhor Diouf; Modou Gueye; Mbayame Niang; Makhtar Camara; Cheikh Saad Bouh Boye

doi:doi:10.11648/j.ijbecs.20190503.12

Ampicillin Resistance in Haemophilus influenzae Isolated from Acute Respiratory Infections in Pediatrics

Abdoulaye Diop, Assane Dieng, Abdoulaye Seck, Amary Fall, Amadou Diop, Djibril Boiro, Jean Baptisse Niokhor Diouf, Modou Gueye, Mbayame Niang, Makhtar Camara, Cheikh Saad Bouh Boye

Published in International Journal of Biomedical Engineering and Clinical Science (Volume 5, Issue 3)

Received: 20 February 2019 Accepted: 10 April 2019 Published: 16 October 2019

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Abstract

Haemophilus influenzae is a bacterium that can cause severe infections, occurring mostly in infants and children younger than five years of age. Antibiotic treatment may cause the emergence of resistant H. influenzae strains, particularly ampicillin-resistant strains. Antimicrobial resistance is a public health threat worldwide, particularly in the developing world. H. influenzae strains have been isolated from broncho-alveolar lavages (BALs), nasopharyngeal swabs, and otitis media from children in two paediatric centers at Dakar, Senegal. Antibiotic susceptibility testing was carried out using strips E Test ^®t method that provides the ability to precisely determine the minimum inhibitory concentration (MIC). A total of 16 H. influenzae strains have been isolated and identified, including 16.7% of ampicillin-resistant patterns (all β-lactamase-negative), 9.4% of the isolates were resistant to cefaclor (MIC₉₀ = 16 µg/ml) while 100% were susceptible to cefixime to (MIC₉₀ = 0.38 µg/ml). Interestingly, fluoroquinolones were fully active with very low MIC₉₀. Macrolide were still active against H. influenzae isoles although with higher MIC azitrhomycin MIC₉₀= 3µg/ml, clarithromycin MIC₉₀= 12µg/ml. Ampicillin-resistance has become increasingly reported in H. influenzae, suggesting a continuous laboratory based surveillance for antimicrobial resistance pattern for a better management of acute respiratory infections, particularly in low incomes settings.

Published in	International Journal of Biomedical Engineering and Clinical Science (Volume 5, Issue 3)
DOI	10.11648/j.ijbecs.20190503.12
Page(s)	40-44
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2019. Published by Science Publishing Group

Keywords

Respiratory Tract Infections, Haemophilus influenzae, Ampicillin Resistance

References

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APA Style

Abdoulaye Diop, Assane Dieng, Abdoulaye Seck, Amary Fall, Amadou Diop, et al. (2019). Ampicillin Resistance in Haemophilus influenzae Isolated from Acute Respiratory Infections in Pediatrics. International Journal of Biomedical Engineering and Clinical Science, 5(3), 40-44. https://doi.org/10.11648/j.ijbecs.20190503.12

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ACS Style

Abdoulaye Diop; Assane Dieng; Abdoulaye Seck; Amary Fall; Amadou Diop, et al. Ampicillin Resistance in Haemophilus influenzae Isolated from Acute Respiratory Infections in Pediatrics. Int. J. Biomed. Eng. Clin. Sci. 2019, 5(3), 40-44. doi: 10.11648/j.ijbecs.20190503.12

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AMA Style

Abdoulaye Diop, Assane Dieng, Abdoulaye Seck, Amary Fall, Amadou Diop, et al. Ampicillin Resistance in Haemophilus influenzae Isolated from Acute Respiratory Infections in Pediatrics. Int J Biomed Eng Clin Sci. 2019;5(3):40-44. doi: 10.11648/j.ijbecs.20190503.12

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@article{10.11648/j.ijbecs.20190503.12,
  author = {Abdoulaye Diop and Assane Dieng and Abdoulaye Seck and Amary Fall and Amadou Diop and Djibril Boiro and Jean Baptisse Niokhor Diouf and Modou Gueye and Mbayame Niang and Makhtar Camara and Cheikh Saad Bouh Boye},
  title = {Ampicillin Resistance in Haemophilus influenzae Isolated from Acute Respiratory Infections in Pediatrics},
  journal = {International Journal of Biomedical Engineering and Clinical Science},
  volume = {5},
  number = {3},
  pages = {40-44},
  doi = {10.11648/j.ijbecs.20190503.12},
  url = {https://doi.org/10.11648/j.ijbecs.20190503.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijbecs.20190503.12},
  abstract = {Haemophilus influenzae is a bacterium that can cause severe infections, occurring mostly in infants and children younger than five years of age. Antibiotic treatment may cause the emergence of resistant H. influenzae strains, particularly ampicillin-resistant strains. Antimicrobial resistance is a public health threat worldwide, particularly in the developing world. H. influenzae strains have been isolated from broncho-alveolar lavages (BALs), nasopharyngeal swabs, and otitis media from children in two paediatric centers at Dakar, Senegal. Antibiotic susceptibility testing was carried out using strips E Test ®t method that provides the ability to precisely determine the minimum inhibitory concentration (MIC). A total of 16 H. influenzae strains have been isolated and identified, including 16.7% of ampicillin-resistant patterns (all β-lactamase-negative), 9.4% of the isolates were resistant to cefaclor (MIC90 = 16 µg/ml) while 100% were susceptible to cefixime to (MIC90 = 0.38 µg/ml). Interestingly, fluoroquinolones were fully active with very low MIC90. Macrolide were still active against H. influenzae isoles although with higher MIC azitrhomycin MIC90= 3µg/ml, clarithromycin MIC90= 12µg/ml. Ampicillin-resistance has become increasingly reported in H. influenzae, suggesting a continuous laboratory based surveillance for antimicrobial resistance pattern for a better management of acute respiratory infections, particularly in low incomes settings.},
 year = {2019}
}

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TY - JOUR
T1 - Ampicillin Resistance in Haemophilus influenzae Isolated from Acute Respiratory Infections in Pediatrics
AU - Abdoulaye Diop
AU - Assane Dieng
AU - Abdoulaye Seck
AU - Amary Fall
AU - Amadou Diop
AU - Djibril Boiro
AU - Jean Baptisse Niokhor Diouf
AU - Modou Gueye
AU - Mbayame Niang
AU - Makhtar Camara
AU - Cheikh Saad Bouh Boye
Y1 - 2019/10/16
PY - 2019
N1 - https://doi.org/10.11648/j.ijbecs.20190503.12
DO - 10.11648/j.ijbecs.20190503.12
T2 - International Journal of Biomedical Engineering and Clinical Science
JF - International Journal of Biomedical Engineering and Clinical Science
JO - International Journal of Biomedical Engineering and Clinical Science
SP - 40
EP - 44
PB - Science Publishing Group
SN - 2472-1301
UR - https://doi.org/10.11648/j.ijbecs.20190503.12
AB - Haemophilus influenzae is a bacterium that can cause severe infections, occurring mostly in infants and children younger than five years of age. Antibiotic treatment may cause the emergence of resistant H. influenzae strains, particularly ampicillin-resistant strains. Antimicrobial resistance is a public health threat worldwide, particularly in the developing world. H. influenzae strains have been isolated from broncho-alveolar lavages (BALs), nasopharyngeal swabs, and otitis media from children in two paediatric centers at Dakar, Senegal. Antibiotic susceptibility testing was carried out using strips E Test ®t method that provides the ability to precisely determine the minimum inhibitory concentration (MIC). A total of 16 H. influenzae strains have been isolated and identified, including 16.7% of ampicillin-resistant patterns (all β-lactamase-negative), 9.4% of the isolates were resistant to cefaclor (MIC90 = 16 µg/ml) while 100% were susceptible to cefixime to (MIC90 = 0.38 µg/ml). Interestingly, fluoroquinolones were fully active with very low MIC90. Macrolide were still active against H. influenzae isoles although with higher MIC azitrhomycin MIC90= 3µg/ml, clarithromycin MIC90= 12µg/ml. Ampicillin-resistance has become increasingly reported in H. influenzae, suggesting a continuous laboratory based surveillance for antimicrobial resistance pattern for a better management of acute respiratory infections, particularly in low incomes settings.
VL - 5
IS - 3
ER -

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Author Information

Abdoulaye Diop

Bacteriology and Virology Laboratory, Le Dantec Teaching Hospital, Dakar, Senegal
Assane Dieng

Bacteriology and Virology Laboratory, Le Dantec Teaching Hospital, Dakar, Senegal
Abdoulaye Seck

Medical Virology Unit, Institute Pasteur, Dakar, Senegal
Amary Fall

Medical Virology Unit, Institute Pasteur, Dakar, Senegal
Amadou Diop

Bacteriology and Virology Laboratory, Le Dantec Teaching Hospital, Dakar, Senegal
Djibril Boiro

Paediatric Unit, Abass NDAO Teaching Hospital, Dakar, Senegal
Jean Baptisse Niokhor Diouf

Paediatric Unit, Roi Baudouin Hospital, Dakar, Senegal
Modou Gueye

Paediatric Unit, Abass NDAO Teaching Hospital, Dakar, Senegal
Mbayame Niang

Medical Virology Unit, Institute Pasteur, Dakar, Senegal
Makhtar Camara

Bacteriology and Virology Laboratory, Le Dantec Teaching Hospital, Dakar, Senegal
Cheikh Saad Bouh Boye

Bacteriology and Virology Laboratory, Le Dantec Teaching Hospital, Dakar, Senegal

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Plain Text BibTeX RIS

APA Style

Abdoulaye Diop, Assane Dieng, Abdoulaye Seck, Amary Fall, Amadou Diop, et al. (2019). Ampicillin Resistance in Haemophilus influenzae Isolated from Acute Respiratory Infections in Pediatrics. International Journal of Biomedical Engineering and Clinical Science, 5(3), 40-44. https://doi.org/10.11648/j.ijbecs.20190503.12

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ACS Style

Abdoulaye Diop; Assane Dieng; Abdoulaye Seck; Amary Fall; Amadou Diop, et al. Ampicillin Resistance in Haemophilus influenzae Isolated from Acute Respiratory Infections in Pediatrics. Int. J. Biomed. Eng. Clin. Sci. 2019, 5(3), 40-44. doi: 10.11648/j.ijbecs.20190503.12

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AMA Style

Abdoulaye Diop, Assane Dieng, Abdoulaye Seck, Amary Fall, Amadou Diop, et al. Ampicillin Resistance in Haemophilus influenzae Isolated from Acute Respiratory Infections in Pediatrics. Int J Biomed Eng Clin Sci. 2019;5(3):40-44. doi: 10.11648/j.ijbecs.20190503.12

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@article{10.11648/j.ijbecs.20190503.12,
  author = {Abdoulaye Diop and Assane Dieng and Abdoulaye Seck and Amary Fall and Amadou Diop and Djibril Boiro and Jean Baptisse Niokhor Diouf and Modou Gueye and Mbayame Niang and Makhtar Camara and Cheikh Saad Bouh Boye},
  title = {Ampicillin Resistance in Haemophilus influenzae Isolated from Acute Respiratory Infections in Pediatrics},
  journal = {International Journal of Biomedical Engineering and Clinical Science},
  volume = {5},
  number = {3},
  pages = {40-44},
  doi = {10.11648/j.ijbecs.20190503.12},
  url = {https://doi.org/10.11648/j.ijbecs.20190503.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijbecs.20190503.12},
  abstract = {Haemophilus influenzae is a bacterium that can cause severe infections, occurring mostly in infants and children younger than five years of age. Antibiotic treatment may cause the emergence of resistant H. influenzae strains, particularly ampicillin-resistant strains. Antimicrobial resistance is a public health threat worldwide, particularly in the developing world. H. influenzae strains have been isolated from broncho-alveolar lavages (BALs), nasopharyngeal swabs, and otitis media from children in two paediatric centers at Dakar, Senegal. Antibiotic susceptibility testing was carried out using strips E Test ®t method that provides the ability to precisely determine the minimum inhibitory concentration (MIC). A total of 16 H. influenzae strains have been isolated and identified, including 16.7% of ampicillin-resistant patterns (all β-lactamase-negative), 9.4% of the isolates were resistant to cefaclor (MIC90 = 16 µg/ml) while 100% were susceptible to cefixime to (MIC90 = 0.38 µg/ml). Interestingly, fluoroquinolones were fully active with very low MIC90. Macrolide were still active against H. influenzae isoles although with higher MIC azitrhomycin MIC90= 3µg/ml, clarithromycin MIC90= 12µg/ml. Ampicillin-resistance has become increasingly reported in H. influenzae, suggesting a continuous laboratory based surveillance for antimicrobial resistance pattern for a better management of acute respiratory infections, particularly in low incomes settings.},
 year = {2019}
}

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