Supercritical Synthesis of Poly (2-Dimethylaminoethyl Methacrylate)/Ferrite Nanocomposites and Online Electrochemical Monitoring of Protein Release

Gunjan Bisht; M. G. H. Zaidi

doi:doi:10.11648/j.ijbmr.20140201.11

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Supercritical Synthesis of Poly (2-Dimethylaminoethyl Methacrylate)/Ferrite Nanocomposites and Online Electrochemical Monitoring of Protein Release

Gunjan Bisht, M. G. H. Zaidi

Published in International Journal of Biomedical Materials Research (Volume 2, Issue 1)

Received: 28 April 2014 Accepted: 3 June 2014 Published: 20 June 2014

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Abstract

A supercritical carbon dioxide (SCC) assisted process was developed to synthesize protein supported poly (2-dimethylaminoethyl methacrylate)/ferrite nanocomposites (PNCs). The process involve 2,2-azobisisobutyronitrile initiated insitu polymerization of 2-dimethylaminoethyl methacrylate in presence of ferrite nanoparticles and bisacrylamide at 90±1 oC, 1200 psi over 6 hr in SCC. This was followed by subsequent loading of bovine serum albumin (BSA) as a model protein over PNCs in phosphate buffer (PBS, pH 7.4) at 1200 psi, 35±1⁰C over additional 2 hr in SCC. The formation of PNCs was ascertained through Ultra violet-visible, Fourier transform-infrared, X-ray diffraction spectra, transmission electron, atomic force microscopy and magnetometry. The developed process extends large scale production of nanomagnetic PNCs suitable as carrier for protein release applications with optimal release properties. The release of protein from PNCs under in vitro in PBS down to nanomolar range with high temporal resolution, speed and reproducibility was quantified through square wave voltammetry.

Published in	International Journal of Biomedical Materials Research (Volume 2, Issue 1)
DOI	10.11648/j.ijbmr.20140201.11
Page(s)	1-6
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2014. Published by Science Publishing Group

Keywords

Supercritical Synthesis, Nanocomposites, Protein Release, Square Wave Voltammetry

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APA Style

Gunjan Bisht, M. G. H. Zaidi. (2014). Supercritical Synthesis of Poly (2-Dimethylaminoethyl Methacrylate)/Ferrite Nanocomposites and Online Electrochemical Monitoring of Protein Release. International Journal of Biomedical Materials Research, 2(1), 1-6. https://doi.org/10.11648/j.ijbmr.20140201.11

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ACS Style

Gunjan Bisht; M. G. H. Zaidi. Supercritical Synthesis of Poly (2-Dimethylaminoethyl Methacrylate)/Ferrite Nanocomposites and Online Electrochemical Monitoring of Protein Release. Int. J. Biomed. Mater. Res. 2014, 2(1), 1-6. doi: 10.11648/j.ijbmr.20140201.11

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AMA Style

Gunjan Bisht, M. G. H. Zaidi. Supercritical Synthesis of Poly (2-Dimethylaminoethyl Methacrylate)/Ferrite Nanocomposites and Online Electrochemical Monitoring of Protein Release. Int J Biomed Mater Res. 2014;2(1):1-6. doi: 10.11648/j.ijbmr.20140201.11

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@article{10.11648/j.ijbmr.20140201.11,
  author = {Gunjan Bisht and M. G. H. Zaidi},
  title = {Supercritical Synthesis of Poly (2-Dimethylaminoethyl Methacrylate)/Ferrite Nanocomposites and Online Electrochemical Monitoring of Protein Release},
  journal = {International Journal of Biomedical Materials Research},
  volume = {2},
  number = {1},
  pages = {1-6},
  doi = {10.11648/j.ijbmr.20140201.11},
  url = {https://doi.org/10.11648/j.ijbmr.20140201.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijbmr.20140201.11},
  abstract = {A supercritical carbon dioxide (SCC) assisted process was developed to synthesize protein supported poly (2-dimethylaminoethyl methacrylate)/ferrite nanocomposites (PNCs). The process involve 2,2-azobisisobutyronitrile initiated insitu polymerization of 2-dimethylaminoethyl methacrylate in presence of ferrite nanoparticles and bisacrylamide at 90±1 oC, 1200 psi over 6 hr in SCC. This was followed by subsequent loading of bovine serum albumin (BSA) as a model protein over PNCs in phosphate buffer (PBS, pH 7.4) at 1200 psi, 35±1⁰C over additional 2 hr in SCC. The formation of PNCs was ascertained through Ultra violet-visible, Fourier transform-infrared, X-ray diffraction spectra, transmission electron, atomic force microscopy and magnetometry. The developed process extends large scale production of nanomagnetic PNCs suitable as carrier for protein release applications with optimal release properties. The release of protein from PNCs under in vitro in PBS down to nanomolar range with high temporal resolution, speed and reproducibility was quantified through square wave voltammetry.},
 year = {2014}
}

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TY - JOUR
T1 - Supercritical Synthesis of Poly (2-Dimethylaminoethyl Methacrylate)/Ferrite Nanocomposites and Online Electrochemical Monitoring of Protein Release
AU - Gunjan Bisht
AU - M. G. H. Zaidi
Y1 - 2014/06/20
PY - 2014
N1 - https://doi.org/10.11648/j.ijbmr.20140201.11
DO - 10.11648/j.ijbmr.20140201.11
T2 - International Journal of Biomedical Materials Research
JF - International Journal of Biomedical Materials Research
JO - International Journal of Biomedical Materials Research
SP - 1
EP - 6
PB - Science Publishing Group
SN - 2330-7579
UR - https://doi.org/10.11648/j.ijbmr.20140201.11
AB - A supercritical carbon dioxide (SCC) assisted process was developed to synthesize protein supported poly (2-dimethylaminoethyl methacrylate)/ferrite nanocomposites (PNCs). The process involve 2,2-azobisisobutyronitrile initiated insitu polymerization of 2-dimethylaminoethyl methacrylate in presence of ferrite nanoparticles and bisacrylamide at 90±1 oC, 1200 psi over 6 hr in SCC. This was followed by subsequent loading of bovine serum albumin (BSA) as a model protein over PNCs in phosphate buffer (PBS, pH 7.4) at 1200 psi, 35±1⁰C over additional 2 hr in SCC. The formation of PNCs was ascertained through Ultra violet-visible, Fourier transform-infrared, X-ray diffraction spectra, transmission electron, atomic force microscopy and magnetometry. The developed process extends large scale production of nanomagnetic PNCs suitable as carrier for protein release applications with optimal release properties. The release of protein from PNCs under in vitro in PBS down to nanomolar range with high temporal resolution, speed and reproducibility was quantified through square wave voltammetry.
VL - 2
IS - 1
ER -

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Author Information

Gunjan Bisht

Department of Natural Science, Kathmandu University, Dhulikhel, Nepal
M. G. H. Zaidi

Department of Chemistry, G. B. Pant University of Agriculture & Technology, Pantnagar, India

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APA Style

Gunjan Bisht, M. G. H. Zaidi. (2014). Supercritical Synthesis of Poly (2-Dimethylaminoethyl Methacrylate)/Ferrite Nanocomposites and Online Electrochemical Monitoring of Protein Release. International Journal of Biomedical Materials Research, 2(1), 1-6. https://doi.org/10.11648/j.ijbmr.20140201.11

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ACS Style

Gunjan Bisht; M. G. H. Zaidi. Supercritical Synthesis of Poly (2-Dimethylaminoethyl Methacrylate)/Ferrite Nanocomposites and Online Electrochemical Monitoring of Protein Release. Int. J. Biomed. Mater. Res. 2014, 2(1), 1-6. doi: 10.11648/j.ijbmr.20140201.11

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AMA Style

Gunjan Bisht, M. G. H. Zaidi. Supercritical Synthesis of Poly (2-Dimethylaminoethyl Methacrylate)/Ferrite Nanocomposites and Online Electrochemical Monitoring of Protein Release. Int J Biomed Mater Res. 2014;2(1):1-6. doi: 10.11648/j.ijbmr.20140201.11

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@article{10.11648/j.ijbmr.20140201.11,
  author = {Gunjan Bisht and M. G. H. Zaidi},
  title = {Supercritical Synthesis of Poly (2-Dimethylaminoethyl Methacrylate)/Ferrite Nanocomposites and Online Electrochemical Monitoring of Protein Release},
  journal = {International Journal of Biomedical Materials Research},
  volume = {2},
  number = {1},
  pages = {1-6},
  doi = {10.11648/j.ijbmr.20140201.11},
  url = {https://doi.org/10.11648/j.ijbmr.20140201.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijbmr.20140201.11},
  abstract = {A supercritical carbon dioxide (SCC) assisted process was developed to synthesize protein supported poly (2-dimethylaminoethyl methacrylate)/ferrite nanocomposites (PNCs). The process involve 2,2-azobisisobutyronitrile initiated insitu polymerization of 2-dimethylaminoethyl methacrylate in presence of ferrite nanoparticles and bisacrylamide at 90±1 oC, 1200 psi over 6 hr in SCC. This was followed by subsequent loading of bovine serum albumin (BSA) as a model protein over PNCs in phosphate buffer (PBS, pH 7.4) at 1200 psi, 35±1⁰C over additional 2 hr in SCC. The formation of PNCs was ascertained through Ultra violet-visible, Fourier transform-infrared, X-ray diffraction spectra, transmission electron, atomic force microscopy and magnetometry. The developed process extends large scale production of nanomagnetic PNCs suitable as carrier for protein release applications with optimal release properties. The release of protein from PNCs under in vitro in PBS down to nanomolar range with high temporal resolution, speed and reproducibility was quantified through square wave voltammetry.},
 year = {2014}
}

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