Comparisons of Release of Several Antibiotics from Antimicrobial Polymer-Coated Allograft Bone Void Filler

Benjamin D. Brooks; Sherry N. Davidoff; David W. Grainger; Amanda E. Brooks

doi:doi:10.11648/j.ijbmr.20130102.11

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Comparisons of Release of Several Antibiotics from Antimicrobial Polymer-Coated Allograft Bone Void Filler

Benjamin D. Brooks, Sherry N. Davidoff, David W. Grainger, Amanda E. Brooks

Published in International Journal of Biomedical Materials Research (Volume 1, Issue 2)

Received: 12 August 2013 Published: 10 January 2014

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Abstract

Osteomyelitis remains a significant complication in orthopedic surgeries. Although infection rates remain steady at 1-3% for primary orthopedic surgeries, overall numbers of orthopedic procedures are increasing, corresponding to earlier and more frequent surgical intervention for an active, aging population. To address this dangerous surgical complication, degradable polycaprolactone (PCL) polymer/antibiotic solutions were coated over allograft bone void filler. Local in vitro release of ciprofloxacin, vancomycin, oxacillin, tobramycin, or rifampicin from a polymer-controlled, antibiotic-releasing bone graft void filler and monitored in vitro allowed the criterion for successful local antibiotic-releasing devices to be expanded. Although each antibiotic exhibited a different release profile based on their formulation and chemical structure, allowing the potential for engineering combinatorial therapy with microbicidal activity, bacterial killing activity in vitro was demonstrated efficacious out to a clinically relevant 8-week time point. In addition to proposing an expanded criterion for successful local antibiotic-releasing devices, this study demonstrates that allograft bone can act as a local, controlled drug release matrix in bone sites. This combination device provides osteoconductive potential in bone voids while mitigating the potential for operatively sourced opportunistic infectious complications during orthopedic repairs as well as primary and revision arthroplasties

Published in	International Journal of Biomedical Materials Research (Volume 1, Issue 2)
DOI	10.11648/j.ijbmr.20130102.11
Page(s)	21-25
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2014. Published by Science Publishing Group

Keywords

Controlled Release, Antibiotic Delivery, Orthopedic Infection, Antibiotic Combination Therapy, Osteomyelitis, Bone Void Fillers, Antimicrobial Allograft, Polycaprolactone

References

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[2]	Landersdorfer CB, Bulitta JB, Kinzig M, Holzgrabe U, Sörgel F. Penetration of Antibacterials into Bone. Clin Pharmacokinet. 2009;48(2):89–124.
[3]	Campoccia D, Montanaro L, Speziale P, Arciola CR. Antibiotic-loaded biomaterials and the risks for the spread of antibiotic resistance following their prophylactic and therapeutic clinical use. Biomaterials. 2010;31(25):6363–77.
[4]	Brooks BD, Brooks AE, Grainger DW. Antimicrobial Medical Devices in Preclinical Development and Clinical Use. In: Moriarty TF, Zaat SAJ, Busscher HJ, editors. Biomater Assoc Infect [Internet]. Springer New York; 2013 [cited 2012 Oct 30]. p. 307–54. Available from: http://www.springerlink.com/content/rp22657g55495648/abstract/
[5]	Lucke M, Schmidmaier G, Sadoni S, Wildemann B, Schiller R, Haas N., et al. Gentamicin coating of metallic implants reduces implant-related osteomyelitis in rats. Bone. 2003 May;32(5):521–31.
[6]	B Burlington. FDA Public Health Notice Potential Hypersensitivity Reactions To Chlorhexidine-Impregnated Medical Devices [Internet]. FDA; 2013 Feb. Available from: http://www.medline.com/wound-skin-care/silvasorb/lit/FDA%20Health%20Notice%20on%20CHG.pdf
[7]	Anagnostakos K, Fürst O, Kelm J. Antibiotic-impregnated PMMA hip spacers: current status. Acta Orthop. 2006;77(4):628–37.
[8]	Kanellakopoulou K, Giamarellos-Bourboulis EJ. Carrier systems for the local delivery of antibiotics in bone infections. Drugs. 2000 Jun;59(6):1223–32.
[9]	Kluin OS, van der Mei HC, Busscher HJ, Neut D. Biodegradable vs non-biodegradable antibiotic delivery devices in the treatment of osteomyelitis. Expert Opin Drug Deliv. 2013 Mar;10(3):341–51.
[10]	Davidoff SN, Call BP, Hogrebe PC, Grainger DW, Brooks AE. A robust method to coat allograft bone with a drug-releasing polymer shell-biomed 2010. Biomed Sci Instrum. 2010;46:184.
[11]	Amanda E. Brooks, Benjamin D. Brooks Sherry N. Davidoff, Paul C. Hogrebe,, Mark A. Fisher, David W. Grainger. Polymer-Controlled Release of Tobramycin from Bone Graft Void Filler. Drug Deliv Transl Res. In Press;
[12]	Grainger DW. Targeted delivery of therapeutics to bone and connective tissues. Adv Drug Deliv Rev. 2012 Sep;64(12):1061–2.
[13]	CLSI. Performance Standards for Antimicrobial Susceptibility Testing; Seventeenth Informational Supplement [Internet]. Clinical Laboratory Standards Institute; 2007 Jan. Available from: http://www.microbiolab-bg.com/CLSI.pdf
[14]	Neut D, van de Belt H, van Horn JR, van der Mei HC, Busscher HJ. The effect of mixing on gentamicin release from polymethylmethacrylate bone cements. Acta Orthop Scand. 2003 Dec;74(6):670–6.
[15]	Van de Belt H, Neut D, Schenk W, van Horn JR, van der Mei HC, Busscher HJ. Gentamicin release from polymethylmethacrylate bone cements and Staphylococcus aureus biofilm formation. Acta Orthop Scand. 2000 Dec;71(6):625–9.

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APA Style

Benjamin D. Brooks, Sherry N. Davidoff, David W. Grainger, Amanda E. Brooks. (2014). Comparisons of Release of Several Antibiotics from Antimicrobial Polymer-Coated Allograft Bone Void Filler. International Journal of Biomedical Materials Research, 1(2), 21-25. https://doi.org/10.11648/j.ijbmr.20130102.11

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ACS Style

Benjamin D. Brooks; Sherry N. Davidoff; David W. Grainger; Amanda E. Brooks. Comparisons of Release of Several Antibiotics from Antimicrobial Polymer-Coated Allograft Bone Void Filler. Int. J. Biomed. Mater. Res. 2014, 1(2), 21-25. doi: 10.11648/j.ijbmr.20130102.11

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AMA Style

Benjamin D. Brooks, Sherry N. Davidoff, David W. Grainger, Amanda E. Brooks. Comparisons of Release of Several Antibiotics from Antimicrobial Polymer-Coated Allograft Bone Void Filler. Int J Biomed Mater Res. 2014;1(2):21-25. doi: 10.11648/j.ijbmr.20130102.11

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@article{10.11648/j.ijbmr.20130102.11,
  author = {Benjamin D. Brooks and Sherry N. Davidoff and David W. Grainger and Amanda E. Brooks},
  title = {Comparisons of Release of Several Antibiotics from Antimicrobial Polymer-Coated Allograft Bone Void Filler},
  journal = {International Journal of Biomedical Materials Research},
  volume = {1},
  number = {2},
  pages = {21-25},
  doi = {10.11648/j.ijbmr.20130102.11},
  url = {https://doi.org/10.11648/j.ijbmr.20130102.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijbmr.20130102.11},
  abstract = {Osteomyelitis remains a significant complication in orthopedic surgeries. Although infection rates remain steady at 1-3% for primary orthopedic surgeries, overall numbers of orthopedic procedures are increasing, corresponding to earlier and more frequent surgical intervention for an active, aging population. To address this dangerous surgical complication, degradable polycaprolactone (PCL) polymer/antibiotic solutions were coated over allograft bone void filler. Local in vitro release of ciprofloxacin, vancomycin, oxacillin, tobramycin, or rifampicin from a polymer-controlled, antibiotic-releasing bone graft void filler and monitored in vitro allowed the criterion for successful local antibiotic-releasing devices to be expanded. Although each antibiotic exhibited a different release profile based on their formulation and chemical structure, allowing the potential for engineering combinatorial therapy with microbicidal activity, bacterial killing activity in vitro was demonstrated efficacious out to a clinically relevant 8-week time point. In addition to proposing an expanded criterion for successful local antibiotic-releasing devices, this study demonstrates that allograft bone can act as a local, controlled drug release matrix in bone sites. This combination device provides osteoconductive potential in bone voids while mitigating the potential for operatively sourced opportunistic infectious complications during orthopedic repairs as well as primary and revision arthroplasties},
 year = {2014}
}

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TY  - JOUR
T1  - Comparisons of Release of Several Antibiotics from Antimicrobial Polymer-Coated Allograft Bone Void Filler
AU  - Benjamin D. Brooks
AU  - Sherry N. Davidoff
AU  - David W. Grainger
AU  - Amanda E. Brooks
Y1  - 2014/01/10
PY  - 2014
N1  - https://doi.org/10.11648/j.ijbmr.20130102.11
DO  - 10.11648/j.ijbmr.20130102.11
T2  - International Journal of Biomedical Materials Research
JF  - International Journal of Biomedical Materials Research
JO  - International Journal of Biomedical Materials Research
SP  - 21
EP  - 25
PB  - Science Publishing Group
SN  - 2330-7579
UR  - https://doi.org/10.11648/j.ijbmr.20130102.11
AB  - Osteomyelitis remains a significant complication in orthopedic surgeries. Although infection rates remain steady at 1-3% for primary orthopedic surgeries, overall numbers of orthopedic procedures are increasing, corresponding to earlier and more frequent surgical intervention for an active, aging population. To address this dangerous surgical complication, degradable polycaprolactone (PCL) polymer/antibiotic solutions were coated over allograft bone void filler. Local in vitro release of ciprofloxacin, vancomycin, oxacillin, tobramycin, or rifampicin from a polymer-controlled, antibiotic-releasing bone graft void filler and monitored in vitro allowed the criterion for successful local antibiotic-releasing devices to be expanded. Although each antibiotic exhibited a different release profile based on their formulation and chemical structure, allowing the potential for engineering combinatorial therapy with microbicidal activity, bacterial killing activity in vitro was demonstrated efficacious out to a clinically relevant 8-week time point. In addition to proposing an expanded criterion for successful local antibiotic-releasing devices, this study demonstrates that allograft bone can act as a local, controlled drug release matrix in bone sites. This combination device provides osteoconductive potential in bone voids while mitigating the potential for operatively sourced opportunistic infectious complications during orthopedic repairs as well as primary and revision arthroplasties
VL  - 1
IS  - 2
ER  -

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Author Information

Benjamin D. Brooks

Department of Pharmaceutics and Pharmaceutical Chemistry, University of Utah, Salt Lake City, UT, USA
Sherry N. Davidoff

Department of Bioengineering, University of Utah, Salt Lake City, UT, USA
David W. Grainger

Department of Pharmaceutics and Pharmaceutical Chemistry, University of Utah, Salt Lake City, UT, USA
Amanda E. Brooks

Department of Pharmaceutics and Pharmaceutical Chemistry, University of Utah, Salt Lake City, UT, USA

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Plain Text BibTeX RIS

APA Style

Benjamin D. Brooks, Sherry N. Davidoff, David W. Grainger, Amanda E. Brooks. (2014). Comparisons of Release of Several Antibiotics from Antimicrobial Polymer-Coated Allograft Bone Void Filler. International Journal of Biomedical Materials Research, 1(2), 21-25. https://doi.org/10.11648/j.ijbmr.20130102.11

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ACS Style

Benjamin D. Brooks; Sherry N. Davidoff; David W. Grainger; Amanda E. Brooks. Comparisons of Release of Several Antibiotics from Antimicrobial Polymer-Coated Allograft Bone Void Filler. Int. J. Biomed. Mater. Res. 2014, 1(2), 21-25. doi: 10.11648/j.ijbmr.20130102.11

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AMA Style

Benjamin D. Brooks, Sherry N. Davidoff, David W. Grainger, Amanda E. Brooks. Comparisons of Release of Several Antibiotics from Antimicrobial Polymer-Coated Allograft Bone Void Filler. Int J Biomed Mater Res. 2014;1(2):21-25. doi: 10.11648/j.ijbmr.20130102.11

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@article{10.11648/j.ijbmr.20130102.11,
  author = {Benjamin D. Brooks and Sherry N. Davidoff and David W. Grainger and Amanda E. Brooks},
  title = {Comparisons of Release of Several Antibiotics from Antimicrobial Polymer-Coated Allograft Bone Void Filler},
  journal = {International Journal of Biomedical Materials Research},
  volume = {1},
  number = {2},
  pages = {21-25},
  doi = {10.11648/j.ijbmr.20130102.11},
  url = {https://doi.org/10.11648/j.ijbmr.20130102.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijbmr.20130102.11},
  abstract = {Osteomyelitis remains a significant complication in orthopedic surgeries. Although infection rates remain steady at 1-3% for primary orthopedic surgeries, overall numbers of orthopedic procedures are increasing, corresponding to earlier and more frequent surgical intervention for an active, aging population. To address this dangerous surgical complication, degradable polycaprolactone (PCL) polymer/antibiotic solutions were coated over allograft bone void filler. Local in vitro release of ciprofloxacin, vancomycin, oxacillin, tobramycin, or rifampicin from a polymer-controlled, antibiotic-releasing bone graft void filler and monitored in vitro allowed the criterion for successful local antibiotic-releasing devices to be expanded. Although each antibiotic exhibited a different release profile based on their formulation and chemical structure, allowing the potential for engineering combinatorial therapy with microbicidal activity, bacterial killing activity in vitro was demonstrated efficacious out to a clinically relevant 8-week time point. In addition to proposing an expanded criterion for successful local antibiotic-releasing devices, this study demonstrates that allograft bone can act as a local, controlled drug release matrix in bone sites. This combination device provides osteoconductive potential in bone voids while mitigating the potential for operatively sourced opportunistic infectious complications during orthopedic repairs as well as primary and revision arthroplasties},
 year = {2014}
}

Copy | Download

TY  - JOUR
T1  - Comparisons of Release of Several Antibiotics from Antimicrobial Polymer-Coated Allograft Bone Void Filler
AU  - Benjamin D. Brooks
AU  - Sherry N. Davidoff
AU  - David W. Grainger
AU  - Amanda E. Brooks
Y1  - 2014/01/10
PY  - 2014
N1  - https://doi.org/10.11648/j.ijbmr.20130102.11
DO  - 10.11648/j.ijbmr.20130102.11
T2  - International Journal of Biomedical Materials Research
JF  - International Journal of Biomedical Materials Research
JO  - International Journal of Biomedical Materials Research
SP  - 21
EP  - 25
PB  - Science Publishing Group
SN  - 2330-7579
UR  - https://doi.org/10.11648/j.ijbmr.20130102.11
AB  - Osteomyelitis remains a significant complication in orthopedic surgeries. Although infection rates remain steady at 1-3% for primary orthopedic surgeries, overall numbers of orthopedic procedures are increasing, corresponding to earlier and more frequent surgical intervention for an active, aging population. To address this dangerous surgical complication, degradable polycaprolactone (PCL) polymer/antibiotic solutions were coated over allograft bone void filler. Local in vitro release of ciprofloxacin, vancomycin, oxacillin, tobramycin, or rifampicin from a polymer-controlled, antibiotic-releasing bone graft void filler and monitored in vitro allowed the criterion for successful local antibiotic-releasing devices to be expanded. Although each antibiotic exhibited a different release profile based on their formulation and chemical structure, allowing the potential for engineering combinatorial therapy with microbicidal activity, bacterial killing activity in vitro was demonstrated efficacious out to a clinically relevant 8-week time point. In addition to proposing an expanded criterion for successful local antibiotic-releasing devices, this study demonstrates that allograft bone can act as a local, controlled drug release matrix in bone sites. This combination device provides osteoconductive potential in bone voids while mitigating the potential for operatively sourced opportunistic infectious complications during orthopedic repairs as well as primary and revision arthroplasties
VL  - 1
IS  - 2
ER  -

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