Affinity Binding Macroporous Monolithic Cryogel as a Matrix for Extracorporeal Apheresis Medical Devices

Wuraola Akande; Lyuba Mikhalovska; Stuart James; Sergey Mikhalovsky

doi:doi:10.11648/j.ijbmr.20150305.11

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Affinity Binding Macroporous Monolithic Cryogel as a Matrix for Extracorporeal Apheresis Medical Devices

Wuraola Akande, Lyuba Mikhalovska, Stuart James, Sergey Mikhalovsky

Published in International Journal of Biomedical Materials Research (Volume 3, Issue 5)

Received: 19 August 2015 Accepted: 30 August 2015 Published: 9 September 2015

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Abstract

Cytapheresis is an extracorporeal separation technique widely used in medicine for elimination of specific classes of blood cells from circulating blood. It has been shown recently to have clinical efficacy in various disease states, such as leukaemia, autoimmune disorders, rheumatoid arthritis, renal allograft rejection and sickle–cell anaemia. The current study was undertaken to produce an affinity-binding column, based upon a macroporous monolithic cryogel with a structure of interconnected pores, with pore size and low flow resistance potentially suitable for use in cytapheresis. The affinity column was produced from poly (2-hydroxyethyl methacrylate) PHEMA cryogels synthesized by free radical polymerization at -12°C. This study involved assessing haemolytic potential, and functionalisation of polymer matrix with biological ligands. Haemolytic potential of poly (2-hydroxyethyl methacrylate) cryogel was established by measuring free haemoglobin after blood filtration through the column. The anti-human albumin (antibody) was chemically coupled to the epoxy derivatised monolithic cryogels and the binding efficiency of anti-human albumin (antibody) to the cryogel was determined. Our results show that approximately 100% of Red blood cells passed through the column with no evidence of haemolysis found in blood eluted. It was found that ~82% of human serum albumin was retained on the monolithic IgG anti-human albumin cryogel matrix. The obtained results suggest that poly (2-hydroxyethyl methacrylate) monolithic cryogel is a non-haemolytic material (haemocompatible matrix) capable of functionalisation with antibody and thus can be an appropriate matrix for use in extracorporeal apheresis system.

Published in	International Journal of Biomedical Materials Research (Volume 3, Issue 5)
DOI	10.11648/j.ijbmr.20150305.11
Page(s)	56-63
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2015. Published by Science Publishing Group

Keywords

Macroporous Cryogel, Poly (2-Hydroxyethyl Methacrylate), Anti-Human Albumin Antibody, Affinity Cryogel, Ligand Immobilisation, Monolithic Adsorbent, Cell Separation, Haemocompatibility

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APA Style

Wuraola Akande, Lyuba Mikhalovska, Stuart James, Sergey Mikhalovsky. (2015). Affinity Binding Macroporous Monolithic Cryogel as a Matrix for Extracorporeal Apheresis Medical Devices. International Journal of Biomedical Materials Research, 3(5), 56-63. https://doi.org/10.11648/j.ijbmr.20150305.11

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ACS Style

Wuraola Akande; Lyuba Mikhalovska; Stuart James; Sergey Mikhalovsky. Affinity Binding Macroporous Monolithic Cryogel as a Matrix for Extracorporeal Apheresis Medical Devices. Int. J. Biomed. Mater. Res. 2015, 3(5), 56-63. doi: 10.11648/j.ijbmr.20150305.11

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AMA Style

Wuraola Akande, Lyuba Mikhalovska, Stuart James, Sergey Mikhalovsky. Affinity Binding Macroporous Monolithic Cryogel as a Matrix for Extracorporeal Apheresis Medical Devices. Int J Biomed Mater Res. 2015;3(5):56-63. doi: 10.11648/j.ijbmr.20150305.11

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@article{10.11648/j.ijbmr.20150305.11,
  author = {Wuraola Akande and Lyuba Mikhalovska and Stuart James and Sergey Mikhalovsky},
  title = {Affinity Binding Macroporous Monolithic Cryogel as a Matrix for Extracorporeal Apheresis Medical Devices},
  journal = {International Journal of Biomedical Materials Research},
  volume = {3},
  number = {5},
  pages = {56-63},
  doi = {10.11648/j.ijbmr.20150305.11},
  url = {https://doi.org/10.11648/j.ijbmr.20150305.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijbmr.20150305.11},
  abstract = {Cytapheresis is an extracorporeal separation technique widely used in medicine for elimination of specific classes of blood cells from circulating blood. It has been shown recently to have clinical efficacy in various disease states, such as leukaemia, autoimmune disorders, rheumatoid arthritis, renal allograft rejection and sickle–cell anaemia. The current study was undertaken to produce an affinity-binding column, based upon a macroporous monolithic cryogel with a structure of interconnected pores, with pore size and low flow resistance potentially suitable for use in cytapheresis. The affinity column was produced from poly (2-hydroxyethyl methacrylate) PHEMA cryogels synthesized by free radical polymerization at -12°C.  This study involved assessing haemolytic potential, and functionalisation of polymer matrix with biological ligands. Haemolytic potential of poly (2-hydroxyethyl methacrylate) cryogel was established by measuring free haemoglobin after blood filtration through the column. The anti-human albumin (antibody) was chemically coupled to the epoxy derivatised monolithic cryogels and the binding efficiency of anti-human albumin (antibody) to the cryogel was determined. Our results show that approximately 100% of Red blood cells passed through the column with no evidence of haemolysis found in blood eluted.  It was found that ~82% of human serum albumin was retained on the monolithic IgG anti-human albumin cryogel matrix. The obtained results suggest that poly (2-hydroxyethyl methacrylate) monolithic cryogel is a non-haemolytic material (haemocompatible matrix) capable of functionalisation with antibody and thus can be an appropriate matrix for use in extracorporeal apheresis system.},
 year = {2015}
}

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TY  - JOUR
T1  - Affinity Binding Macroporous Monolithic Cryogel as a Matrix for Extracorporeal Apheresis Medical Devices
AU  - Wuraola Akande
AU  - Lyuba Mikhalovska
AU  - Stuart James
AU  - Sergey Mikhalovsky
Y1  - 2015/09/09
PY  - 2015
N1  - https://doi.org/10.11648/j.ijbmr.20150305.11
DO  - 10.11648/j.ijbmr.20150305.11
T2  - International Journal of Biomedical Materials Research
JF  - International Journal of Biomedical Materials Research
JO  - International Journal of Biomedical Materials Research
SP  - 56
EP  - 63
PB  - Science Publishing Group
SN  - 2330-7579
UR  - https://doi.org/10.11648/j.ijbmr.20150305.11
AB  - Cytapheresis is an extracorporeal separation technique widely used in medicine for elimination of specific classes of blood cells from circulating blood. It has been shown recently to have clinical efficacy in various disease states, such as leukaemia, autoimmune disorders, rheumatoid arthritis, renal allograft rejection and sickle–cell anaemia. The current study was undertaken to produce an affinity-binding column, based upon a macroporous monolithic cryogel with a structure of interconnected pores, with pore size and low flow resistance potentially suitable for use in cytapheresis. The affinity column was produced from poly (2-hydroxyethyl methacrylate) PHEMA cryogels synthesized by free radical polymerization at -12°C.  This study involved assessing haemolytic potential, and functionalisation of polymer matrix with biological ligands. Haemolytic potential of poly (2-hydroxyethyl methacrylate) cryogel was established by measuring free haemoglobin after blood filtration through the column. The anti-human albumin (antibody) was chemically coupled to the epoxy derivatised monolithic cryogels and the binding efficiency of anti-human albumin (antibody) to the cryogel was determined. Our results show that approximately 100% of Red blood cells passed through the column with no evidence of haemolysis found in blood eluted.  It was found that ~82% of human serum albumin was retained on the monolithic IgG anti-human albumin cryogel matrix. The obtained results suggest that poly (2-hydroxyethyl methacrylate) monolithic cryogel is a non-haemolytic material (haemocompatible matrix) capable of functionalisation with antibody and thus can be an appropriate matrix for use in extracorporeal apheresis system.
VL  - 3
IS  - 5
ER  -

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Author Information

Wuraola Akande

Biomaterials and Medical Devices Research Group, School of Pharmacy and Biomolecular Sciences, Huxley Building, University of Brighton, Brighton, UK
Lyuba Mikhalovska

Biomaterials and Medical Devices Research Group, School of Pharmacy and Biomolecular Sciences, Huxley Building, University of Brighton, Brighton, UK
Stuart James

Biomaterials and Medical Devices Research Group, School of Pharmacy and Biomolecular Sciences, Huxley Building, University of Brighton, Brighton, UK
Sergey Mikhalovsky

Biomaterials and Medical Devices Research Group, School of Pharmacy and Biomolecular Sciences, Huxley Building, University of Brighton, Brighton, UK

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APA Style

Wuraola Akande, Lyuba Mikhalovska, Stuart James, Sergey Mikhalovsky. (2015). Affinity Binding Macroporous Monolithic Cryogel as a Matrix for Extracorporeal Apheresis Medical Devices. International Journal of Biomedical Materials Research, 3(5), 56-63. https://doi.org/10.11648/j.ijbmr.20150305.11

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ACS Style

Wuraola Akande; Lyuba Mikhalovska; Stuart James; Sergey Mikhalovsky. Affinity Binding Macroporous Monolithic Cryogel as a Matrix for Extracorporeal Apheresis Medical Devices. Int. J. Biomed. Mater. Res. 2015, 3(5), 56-63. doi: 10.11648/j.ijbmr.20150305.11

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AMA Style

Wuraola Akande, Lyuba Mikhalovska, Stuart James, Sergey Mikhalovsky. Affinity Binding Macroporous Monolithic Cryogel as a Matrix for Extracorporeal Apheresis Medical Devices. Int J Biomed Mater Res. 2015;3(5):56-63. doi: 10.11648/j.ijbmr.20150305.11

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@article{10.11648/j.ijbmr.20150305.11,
  author = {Wuraola Akande and Lyuba Mikhalovska and Stuart James and Sergey Mikhalovsky},
  title = {Affinity Binding Macroporous Monolithic Cryogel as a Matrix for Extracorporeal Apheresis Medical Devices},
  journal = {International Journal of Biomedical Materials Research},
  volume = {3},
  number = {5},
  pages = {56-63},
  doi = {10.11648/j.ijbmr.20150305.11},
  url = {https://doi.org/10.11648/j.ijbmr.20150305.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijbmr.20150305.11},
  abstract = {Cytapheresis is an extracorporeal separation technique widely used in medicine for elimination of specific classes of blood cells from circulating blood. It has been shown recently to have clinical efficacy in various disease states, such as leukaemia, autoimmune disorders, rheumatoid arthritis, renal allograft rejection and sickle–cell anaemia. The current study was undertaken to produce an affinity-binding column, based upon a macroporous monolithic cryogel with a structure of interconnected pores, with pore size and low flow resistance potentially suitable for use in cytapheresis. The affinity column was produced from poly (2-hydroxyethyl methacrylate) PHEMA cryogels synthesized by free radical polymerization at -12°C.  This study involved assessing haemolytic potential, and functionalisation of polymer matrix with biological ligands. Haemolytic potential of poly (2-hydroxyethyl methacrylate) cryogel was established by measuring free haemoglobin after blood filtration through the column. The anti-human albumin (antibody) was chemically coupled to the epoxy derivatised monolithic cryogels and the binding efficiency of anti-human albumin (antibody) to the cryogel was determined. Our results show that approximately 100% of Red blood cells passed through the column with no evidence of haemolysis found in blood eluted.  It was found that ~82% of human serum albumin was retained on the monolithic IgG anti-human albumin cryogel matrix. The obtained results suggest that poly (2-hydroxyethyl methacrylate) monolithic cryogel is a non-haemolytic material (haemocompatible matrix) capable of functionalisation with antibody and thus can be an appropriate matrix for use in extracorporeal apheresis system.},
 year = {2015}
}

Copy | Download

TY  - JOUR
T1  - Affinity Binding Macroporous Monolithic Cryogel as a Matrix for Extracorporeal Apheresis Medical Devices
AU  - Wuraola Akande
AU  - Lyuba Mikhalovska
AU  - Stuart James
AU  - Sergey Mikhalovsky
Y1  - 2015/09/09
PY  - 2015
N1  - https://doi.org/10.11648/j.ijbmr.20150305.11
DO  - 10.11648/j.ijbmr.20150305.11
T2  - International Journal of Biomedical Materials Research
JF  - International Journal of Biomedical Materials Research
JO  - International Journal of Biomedical Materials Research
SP  - 56
EP  - 63
PB  - Science Publishing Group
SN  - 2330-7579
UR  - https://doi.org/10.11648/j.ijbmr.20150305.11
AB  - Cytapheresis is an extracorporeal separation technique widely used in medicine for elimination of specific classes of blood cells from circulating blood. It has been shown recently to have clinical efficacy in various disease states, such as leukaemia, autoimmune disorders, rheumatoid arthritis, renal allograft rejection and sickle–cell anaemia. The current study was undertaken to produce an affinity-binding column, based upon a macroporous monolithic cryogel with a structure of interconnected pores, with pore size and low flow resistance potentially suitable for use in cytapheresis. The affinity column was produced from poly (2-hydroxyethyl methacrylate) PHEMA cryogels synthesized by free radical polymerization at -12°C.  This study involved assessing haemolytic potential, and functionalisation of polymer matrix with biological ligands. Haemolytic potential of poly (2-hydroxyethyl methacrylate) cryogel was established by measuring free haemoglobin after blood filtration through the column. The anti-human albumin (antibody) was chemically coupled to the epoxy derivatised monolithic cryogels and the binding efficiency of anti-human albumin (antibody) to the cryogel was determined. Our results show that approximately 100% of Red blood cells passed through the column with no evidence of haemolysis found in blood eluted.  It was found that ~82% of human serum albumin was retained on the monolithic IgG anti-human albumin cryogel matrix. The obtained results suggest that poly (2-hydroxyethyl methacrylate) monolithic cryogel is a non-haemolytic material (haemocompatible matrix) capable of functionalisation with antibody and thus can be an appropriate matrix for use in extracorporeal apheresis system.
VL  - 3
IS  - 5
ER  -

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