The insertion\ deletion (ID) polymorphism in intron 16 of the ACE gene is a marker for a functional polymorphism, and it is also reported to influence levels of ACE in healthy subjects. ACE ID polymorphism is associated with an elevated risk of thrombosis and may be causally associated with coronary heart disease (CHD). To investigate the association between ACE gene polymorphism and CHD in the Gaza strip population, we conducted case-control study included 260 samples comprised 160 CHD patients and 100 control subjects. Questionnaire interview was applied. Blood samples were collected in EDTA tube for DNA extraction. Polymerase Chain Reaction (PCR) used to detect ACE ID polymorphism. There was significant association between CHD and age, physical activity, education level, occupation, and family history of CHD. No significant association was observed between CHD and gender and smoking. The genotype frequencies were: ID (36.9%, 42%), DD (53.8%, 54%) and II (9.4%, 4%) in case and control groups respectively. The ACE ID allele frequencies were: I (27%, 25%) and D (72.2%, 75%) in case and control group respectively. The D allele was the most frequent allele in both groups. No association between ACE ID polymorphism and gender. The DD genotype was the most frequent in both study groups. There was no statistically significant association between the ACE ID polymorphism and CHD in Gaza strip. The results showed that there was no significant association between the ACE ID gene polymorphism and CHD in Gaza strip.
| Published in | International Journal of Biomedical Materials Research (Volume 4, Issue 3) |
| DOI | 10.11648/j.ijbmr.20160403.12 |
| Page(s) | 18-26 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2016. Published by Science Publishing Group |
Angiotensin Converting Enzyme Gene, Coronary Heart Disease, Polymerase Chain Reaction, Polymorphism
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APA Style
Lamia'a Sobhi. Saqer, Haneen Ahmad. Khammash, Eman Loai. Shurrab, Mariam Nahed. Aabed, Rana Afif. El-Malakh. (2016). Association Between Angiotensin Converting Enzyme Gene Insertion\Deletion Polymorphism and Coronary Heart Disease in Gaza Strip. International Journal of Biomedical Materials Research, 4(3), 18-26. https://doi.org/10.11648/j.ijbmr.20160403.12
ACS Style
Lamia'a Sobhi. Saqer; Haneen Ahmad. Khammash; Eman Loai. Shurrab; Mariam Nahed. Aabed; Rana Afif. El-Malakh. Association Between Angiotensin Converting Enzyme Gene Insertion\Deletion Polymorphism and Coronary Heart Disease in Gaza Strip. Int. J. Biomed. Mater. Res. 2016, 4(3), 18-26. doi: 10.11648/j.ijbmr.20160403.12
AMA Style
Lamia'a Sobhi. Saqer, Haneen Ahmad. Khammash, Eman Loai. Shurrab, Mariam Nahed. Aabed, Rana Afif. El-Malakh. Association Between Angiotensin Converting Enzyme Gene Insertion\Deletion Polymorphism and Coronary Heart Disease in Gaza Strip. Int J Biomed Mater Res. 2016;4(3):18-26. doi: 10.11648/j.ijbmr.20160403.12
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Download@article{10.11648/j.ijbmr.20160403.12,
author = {Lamia'a Sobhi. Saqer and Haneen Ahmad. Khammash and Eman Loai. Shurrab and Mariam Nahed. Aabed and Rana Afif. El-Malakh},
title = {Association Between Angiotensin Converting Enzyme Gene Insertion\Deletion Polymorphism and Coronary Heart Disease in Gaza Strip},
journal = {International Journal of Biomedical Materials Research},
volume = {4},
number = {3},
pages = {18-26},
doi = {10.11648/j.ijbmr.20160403.12},
url = {https://doi.org/10.11648/j.ijbmr.20160403.12},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijbmr.20160403.12},
abstract = {The insertion\ deletion (ID) polymorphism in intron 16 of the ACE gene is a marker for a functional polymorphism, and it is also reported to influence levels of ACE in healthy subjects. ACE ID polymorphism is associated with an elevated risk of thrombosis and may be causally associated with coronary heart disease (CHD). To investigate the association between ACE gene polymorphism and CHD in the Gaza strip population, we conducted case-control study included 260 samples comprised 160 CHD patients and 100 control subjects. Questionnaire interview was applied. Blood samples were collected in EDTA tube for DNA extraction. Polymerase Chain Reaction (PCR) used to detect ACE ID polymorphism. There was significant association between CHD and age, physical activity, education level, occupation, and family history of CHD. No significant association was observed between CHD and gender and smoking. The genotype frequencies were: ID (36.9%, 42%), DD (53.8%, 54%) and II (9.4%, 4%) in case and control groups respectively. The ACE ID allele frequencies were: I (27%, 25%) and D (72.2%, 75%) in case and control group respectively. The D allele was the most frequent allele in both groups. No association between ACE ID polymorphism and gender. The DD genotype was the most frequent in both study groups. There was no statistically significant association between the ACE ID polymorphism and CHD in Gaza strip. The results showed that there was no significant association between the ACE ID gene polymorphism and CHD in Gaza strip.},
year = {2016}
}
TY - JOUR T1 - Association Between Angiotensin Converting Enzyme Gene Insertion\Deletion Polymorphism and Coronary Heart Disease in Gaza Strip AU - Lamia'a Sobhi. Saqer AU - Haneen Ahmad. Khammash AU - Eman Loai. Shurrab AU - Mariam Nahed. Aabed AU - Rana Afif. El-Malakh Y1 - 2016/11/10 PY - 2016 N1 - https://doi.org/10.11648/j.ijbmr.20160403.12 DO - 10.11648/j.ijbmr.20160403.12 T2 - International Journal of Biomedical Materials Research JF - International Journal of Biomedical Materials Research JO - International Journal of Biomedical Materials Research SP - 18 EP - 26 PB - Science Publishing Group SN - 2330-7579 UR - https://doi.org/10.11648/j.ijbmr.20160403.12 AB - The insertion\ deletion (ID) polymorphism in intron 16 of the ACE gene is a marker for a functional polymorphism, and it is also reported to influence levels of ACE in healthy subjects. ACE ID polymorphism is associated with an elevated risk of thrombosis and may be causally associated with coronary heart disease (CHD). To investigate the association between ACE gene polymorphism and CHD in the Gaza strip population, we conducted case-control study included 260 samples comprised 160 CHD patients and 100 control subjects. Questionnaire interview was applied. Blood samples were collected in EDTA tube for DNA extraction. Polymerase Chain Reaction (PCR) used to detect ACE ID polymorphism. There was significant association between CHD and age, physical activity, education level, occupation, and family history of CHD. No significant association was observed between CHD and gender and smoking. The genotype frequencies were: ID (36.9%, 42%), DD (53.8%, 54%) and II (9.4%, 4%) in case and control groups respectively. The ACE ID allele frequencies were: I (27%, 25%) and D (72.2%, 75%) in case and control group respectively. The D allele was the most frequent allele in both groups. No association between ACE ID polymorphism and gender. The DD genotype was the most frequent in both study groups. There was no statistically significant association between the ACE ID polymorphism and CHD in Gaza strip. The results showed that there was no significant association between the ACE ID gene polymorphism and CHD in Gaza strip. VL - 4 IS - 3 ER -
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