The Role of Cymbopogon Citratus Extract in Protecting the Liver Against Injurious Effect of Dimethylnitrosamine in Rats

Naglaa Ali Saber Sarg; Usama Fouad Ahmed; Ali Mohamed Ali; Saleh Sayed Idris

doi:doi:10.11648/j.ijcda.20150104.12

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The Role of Cymbopogon Citratus Extract in Protecting the Liver Against Injurious Effect of Dimethylnitrosamine in Rats

Naglaa Ali Saber Sarg, Usama Fouad Ahmed, Ali Mohamed Ali, Saleh Sayed Idris

Published in International Journal of Clinical and Developmental Anatomy (Volume 1, Issue 4)

Received: 7 February 2016 Accepted: 14 February 2016 Published: 26 February 2016

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Abstract

Background: N-Nitrosodimethylamine (NDMA) is a potent hepatotoxin that induces fibrosis of the liver. Cymbopogon citratus, a plant that is widely cultivated to be eaten either fresh with food or dried in tea or soft drink has been reported to possess a number of medicinal and aromatic properties. Aim of the work: This work is aimed to study the protective effect of Cymbopogon citratus ethanolic leaf extract against hepatic injury and fibrosis induced by repeated intermittent administration of Dimethylnitrosamine (DMN) in rats. Materials and methods: A total of 30 rats divided into 3 groups were used. Group 1 served as control, Group 2 was given intraperitoneal injection with DMA at a concentration of 10mg/kg body weight on three consecutive days of each week over a period of three weeks. Group 3 was given DMA at a concentration of 10mg/kg body weight on three consecutive days of each week over a period of three weeks and 100 mg/kg body weight of Cymbopogon citratus ethanolic aqueous leaf extract 5 hours after administration of DMN. Results: It was found that DMN produces histopathological changes of the liver; including signs of severe hepatic injury. The cytoplasm of most hepatocytes appeared vacuolated with pyknotic and karyolytic nuclei, congested blood vessels and focal necrotic areas with marked increase of the collagen fibers deposition around the blood vessels. Ultrastructural changes showed Pyknotic nuclei, vacuolated cytoplasm and reduction or complete loss of cristae in the mitochondria. These were reversed by simultaneous treatment with Cymbopogon citratus. In conclusion: The results of this present study indicated that aqueous leaf extracts of Cymbopogon citratus has an antihepatotoxic action against DMN induced hepatic oxidative damage in rats which might be ascribed to its antioxidant and free radical scavenging property.

Published in	International Journal of Clinical and Developmental Anatomy (Volume 1, Issue 4)
DOI	10.11648/j.ijcda.20150104.12
Page(s)	89-95
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2016. Published by Science Publishing Group

Keywords

Dimethylnitrosmine, Fibrosis, Liver, Rat, Cymbopogon Citratus

References

[1]	Lima CF, Fernandes-Ferreira M and Pereira-Wilson C (2007): Drinking of Salvia officinalis tea increases CCl4-induced hepatotoxicity in mice. Food Chem Toxicol 45 (3): 456-464.
[2]	Bataller R and Brenner DA (2005): Liver fibrosis. J Clin Invest 115 (2): 209-218.
[3]	Caldwell S and Park SH (2009): The epidemiology of hepatocellular cancer: from the perspectives of public health problem to tumor biology. J Gastroenterol 44 (suppl. 19): 96-101.
[4]	Vendemiale G, Grattagliano I, Caruso ML, Serviddio G and Valentini AM (2001): Increased oxidative stress in dimethylnitrosamine-induced liver fibrosis in the rat: effect of N-acetylcysteine and interferon-alpha. Toxicol Appl Pharm acol 175 (2): 130-139.
[5]	David L. Sedlak, Rula A. Deeb, Elisabeth L. Hawley, William A. Mitch, Timothy D. Durbin, Sam Mowbray and Steve Carr(2005): Sources and Fate of Nitrosodimethylamine and Its Precursors in Municipal Wastewater Treatment Plants Water Environment Research Vol. 77, No., pp. 32-39.
[6]	Mitch, W. A.; Sharp, J. O.; Trussell, R. R.; Valentine, R. L.; Alvarez-Cohen, Land Sedlak, D. L. (2003): N-Nitrosodimethylamine (NDMA) as a Drinking Water Contaminant. Environmental Engineering Science 20 (5): 389–404.
[7]	Najm, I.; Trussell, R. R. (2001): NDMA Formation in Water and Wastewater. Journal American Water Works Association 93 (2): 92–99.
[8]	George J, Rao KR, Stern R and Chandrakasan G (2001): Dimethylnitrosamine induced liver injury in rats: the early deposition of collagen. Toxicology 156 (2-3): 129-138.
[9]	Choi JH, Kim DW, Yun N, Choi JS and Islam MN (2011): Protective effects of hyperoside against carbon tetrachloride-induced liver damage in mice. J Nat Prod 74: 1055-1060.
[10]	Saleh Muhammad Rahim, Ekhlass Muhi Taha2, Muneef Saeb Al-janabi1, Bushra Ismael Al-douri1, Kumar Das Simon3, Abd Gaffar Mazlan3 (2014): Hepatoprotective Effect Of Cymbopogon Citratus Aqueous Extract Against Hydrogen Peroxide-Induced Liver Injury In Male Rats. Afr J Tradit Complement Altern Med. 11(2): 447-451.
[11]	Bancroft J. D. and Gamble M. (2008): Theory and practice of histological techniques. 6th ed. Churchill Livingstone. London, New York & Sydney, P. 121-132.
[12]	Horbin E. and Bancroft, J. D. (1998): Trouble shooting histology stain, PAS technique. Churchill, Livingstone, New York. P. 154.
[13]	Leong, A. S. (1996): Principles and practice of medical laboratory science. Volume 1: Basic Histotechnology. 1st ed., Philadelphia, Saunders Company. P. 171.
[14]	Ahmad R. (2009): Operculina turpethum attenuates N-nitrosodimethylamine induced toxic liver injury and clastogenicity in rats. Chem Biol Int.; 181: 145–153.
[15]	Usunomena U, Okolie N. Paulinus and Eze I. Gerald (2015): Inhibitory Effect of Vernonia amygdalina on Dimethylnitrosamine (DMN)-induced Liver Fibrosis in Rats. Int J Clin Pharmacol Toxicol, 4(4) 179-184.
[16]	Sharma, V and Singh M. (2014): Attenuation of nitrosodimethylamine induced hepatotoxicity by Operculina turpethum in Swiss Albino mice. Iran J Basic Med Sci. Jan; 17(1): 73–80.
[17]	Ezhilarasan, D. Karthikeyan, S. and Palani Vivekanandan (2012): Ameliorative effect of silibinin against N-nitrosodimethylamine-induced hepatic fibrosis in rats. Environmental toxicology and pharmacology. (34)1004–1013.
[18]	Sakr S. A., Abdel Samei H. A. and Soliman M. E. (2004): Exploring hepatotoxicity of benomyl. Histological and histochemical study on albino rats J Med Sci; 4: 77–83.
[19]	El-Roby N. M. (1994): Histological and Histochemical Effects of Some Steroid Hormones on the Liver of Male Rats. Thesis of M. D. Cairo University. 56-88.
[20]	Pradeep, K.; Raj Mohan, C. V.; Gobianand, K and Karthikeyan, S. (2010): Protective effect of Cassia fistula Linn. on diethylnitrosamine induced hepatocellular damage and oxidative stress in ethanol pretreated rats. Biol. Res., 43(1): 113-25.
[21]	Kass, G. E. (2006): Mitochondrial involvement in drug-induced hepatic injury. Chem – Biol. Interact., 163: 145-159.
[22]	Boelsterli, U. A., and Lim, P. L. (2007): Mitochondrial abnormalities - a link to idiosyncratic drug hepatotoxicity. Toxicol. Appl. Pharmacol., 220: 92-107.
[23]	Gowtham Kumar Subbaraj, Langeswaran Kulanthaivel, Revathy Rajendran Ramakrishnan Veeraba Thiran (2013): Ethanolic Extract of Carum Carvi (Eecc) Prevents N-Nitrosodiethylamine Induced Phenobarbital Promoted Hepatocarcinogenesis By Modulating Antioxidant Enzymes. International Journal of Pharmacy And Pharmaceutical Sciences Issn-0975-1491 Vol 5, Suppl 1.
[24]	Pessayre D1, Mansouri A, Berson A and Fromenty B. (2010): Mitochondrial involvement in drug-induced liver injury. Handb Exp Pharmacol; (196): 311-65.
[25]	Pei HK, Mokhtar RA and Mohammad I (2012): Antioxidant potential of Cymbopogon citratus extract: alleviation of carbontetrachloride-induced hepatic oxidative stress and toxicity. Hum. Exp. Toxicol. 311: 81-91.
[26]	Koh, PH1, Mokhtar RA, Iqbal M. (2012): Antioxidant potential of Cymbopogon citratus extract: alleviation of carbon tetrachloride-induced hepatic oxidative stress and toxicity. Hum Exp Toxicol Jan; 31(1): 81-91.
[27]	Omotade IO (2009): Chemical profile and antimicrobial activity of Cymbopogon citratus leaves. Journal of Natural Products, 2: 98-103.
[28]	Ramanathan R, Luu K and Das NP (1989): Anti peroxidative action of flavonoids and related products in ground pork Abstracts proceedings of III International Symposium on flavonoids in Biology and Medicine. Singapore. p. 56.
[29]	Robak k and Grygleuski RJ (1988): Flavonoids are scavengers of superoxide anions. Biochem. Pharmacol. 37: 837-841.
[30]	Singh, N., Verma, P., Misharu, N. and Nath R (1991): A comparative evaluation of some antistress agents of plant origin. Indian J. Pharmacol. 21: 99.

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APA Style

Naglaa Ali Saber Sarg, Usama Fouad Ahmed, Ali Mohamed Ali, Saleh Sayed Idris. (2016). The Role of Cymbopogon Citratus Extract in Protecting the Liver Against Injurious Effect of Dimethylnitrosamine in Rats. International Journal of Clinical and Developmental Anatomy, 1(4), 89-95. https://doi.org/10.11648/j.ijcda.20150104.12

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ACS Style

Naglaa Ali Saber Sarg; Usama Fouad Ahmed; Ali Mohamed Ali; Saleh Sayed Idris. The Role of Cymbopogon Citratus Extract in Protecting the Liver Against Injurious Effect of Dimethylnitrosamine in Rats. Int. J. Clin. Dev. Anat. 2016, 1(4), 89-95. doi: 10.11648/j.ijcda.20150104.12

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AMA Style

Naglaa Ali Saber Sarg, Usama Fouad Ahmed, Ali Mohamed Ali, Saleh Sayed Idris. The Role of Cymbopogon Citratus Extract in Protecting the Liver Against Injurious Effect of Dimethylnitrosamine in Rats. Int J Clin Dev Anat. 2016;1(4):89-95. doi: 10.11648/j.ijcda.20150104.12

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@article{10.11648/j.ijcda.20150104.12,
  author = {Naglaa Ali Saber Sarg and Usama Fouad Ahmed and Ali Mohamed Ali and Saleh Sayed Idris},
  title = {The Role of Cymbopogon Citratus Extract in Protecting the Liver Against Injurious Effect of Dimethylnitrosamine in Rats},
  journal = {International Journal of Clinical and Developmental Anatomy},
  volume = {1},
  number = {4},
  pages = {89-95},
  doi = {10.11648/j.ijcda.20150104.12},
  url = {https://doi.org/10.11648/j.ijcda.20150104.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijcda.20150104.12},
  abstract = {Background: N-Nitrosodimethylamine (NDMA) is a potent hepatotoxin that induces fibrosis of the liver. Cymbopogon citratus, a plant that is widely cultivated to be eaten either fresh with food or dried in tea or soft drink has been reported to possess a number of medicinal and aromatic properties. Aim of the work: This work is aimed to study the protective effect of Cymbopogon citratus ethanolic leaf extract against hepatic injury and fibrosis induced by repeated intermittent administration of Dimethylnitrosamine (DMN) in rats. Materials and methods: A total of 30 rats divided into 3 groups were used. Group 1 served as control, Group 2 was given intraperitoneal injection with DMA at a concentration of 10mg/kg body weight on three consecutive days of each week over a period of three weeks. Group 3 was given DMA at a concentration of 10mg/kg body weight on three consecutive days of each week over a period of three weeks and 100 mg/kg body weight of Cymbopogon citratus ethanolic aqueous leaf extract 5 hours after administration of DMN. Results: It was found that DMN produces histopathological changes of the liver; including signs of severe hepatic injury. The cytoplasm of most hepatocytes appeared vacuolated with pyknotic and karyolytic nuclei, congested blood vessels and focal necrotic areas with marked increase of the collagen fibers deposition around the blood vessels. Ultrastructural changes showed Pyknotic nuclei, vacuolated cytoplasm and reduction or complete loss of cristae in the mitochondria. These were reversed by simultaneous treatment with Cymbopogon citratus. In conclusion: The results of this present study indicated that aqueous leaf extracts of Cymbopogon citratus has an antihepatotoxic action against DMN induced hepatic oxidative damage in rats which might be ascribed to its antioxidant and free radical scavenging property.},
 year = {2016}
}

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TY  - JOUR
T1  - The Role of Cymbopogon Citratus Extract in Protecting the Liver Against Injurious Effect of Dimethylnitrosamine in Rats
AU  - Naglaa Ali Saber Sarg
AU  - Usama Fouad Ahmed
AU  - Ali Mohamed Ali
AU  - Saleh Sayed Idris
Y1  - 2016/02/26
PY  - 2016
N1  - https://doi.org/10.11648/j.ijcda.20150104.12
DO  - 10.11648/j.ijcda.20150104.12
T2  - International Journal of Clinical and Developmental Anatomy
JF  - International Journal of Clinical and Developmental Anatomy
JO  - International Journal of Clinical and Developmental Anatomy
SP  - 89
EP  - 95
PB  - Science Publishing Group
SN  - 2469-8008
UR  - https://doi.org/10.11648/j.ijcda.20150104.12
AB  - Background: N-Nitrosodimethylamine (NDMA) is a potent hepatotoxin that induces fibrosis of the liver. Cymbopogon citratus, a plant that is widely cultivated to be eaten either fresh with food or dried in tea or soft drink has been reported to possess a number of medicinal and aromatic properties. Aim of the work: This work is aimed to study the protective effect of Cymbopogon citratus ethanolic leaf extract against hepatic injury and fibrosis induced by repeated intermittent administration of Dimethylnitrosamine (DMN) in rats. Materials and methods: A total of 30 rats divided into 3 groups were used. Group 1 served as control, Group 2 was given intraperitoneal injection with DMA at a concentration of 10mg/kg body weight on three consecutive days of each week over a period of three weeks. Group 3 was given DMA at a concentration of 10mg/kg body weight on three consecutive days of each week over a period of three weeks and 100 mg/kg body weight of Cymbopogon citratus ethanolic aqueous leaf extract 5 hours after administration of DMN. Results: It was found that DMN produces histopathological changes of the liver; including signs of severe hepatic injury. The cytoplasm of most hepatocytes appeared vacuolated with pyknotic and karyolytic nuclei, congested blood vessels and focal necrotic areas with marked increase of the collagen fibers deposition around the blood vessels. Ultrastructural changes showed Pyknotic nuclei, vacuolated cytoplasm and reduction or complete loss of cristae in the mitochondria. These were reversed by simultaneous treatment with Cymbopogon citratus. In conclusion: The results of this present study indicated that aqueous leaf extracts of Cymbopogon citratus has an antihepatotoxic action against DMN induced hepatic oxidative damage in rats which might be ascribed to its antioxidant and free radical scavenging property.
VL  - 1
IS  - 4
ER  -

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Author Information

Naglaa Ali Saber Sarg

Department of Anatomy, Faculty of Medicine, Benha University, Benha City, Egypt
Usama Fouad Ahmed

Department of Anatomy, Faculty of Medicine, Benha University, Benha City, Egypt
Ali Mohamed Ali

Department of Anatomy, Faculty of Medicine, Benha University, Benha City, Egypt
Saleh Sayed Idris

Department of Health Education & Health Promotion, Umm Al-Qura University, Makkah City, Saudi Arabia

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Naglaa Ali Saber Sarg, Usama Fouad Ahmed, Ali Mohamed Ali, Saleh Sayed Idris. (2016). The Role of Cymbopogon Citratus Extract in Protecting the Liver Against Injurious Effect of Dimethylnitrosamine in Rats. International Journal of Clinical and Developmental Anatomy, 1(4), 89-95. https://doi.org/10.11648/j.ijcda.20150104.12

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ACS Style

Naglaa Ali Saber Sarg; Usama Fouad Ahmed; Ali Mohamed Ali; Saleh Sayed Idris. The Role of Cymbopogon Citratus Extract in Protecting the Liver Against Injurious Effect of Dimethylnitrosamine in Rats. Int. J. Clin. Dev. Anat. 2016, 1(4), 89-95. doi: 10.11648/j.ijcda.20150104.12

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AMA Style

Naglaa Ali Saber Sarg, Usama Fouad Ahmed, Ali Mohamed Ali, Saleh Sayed Idris. The Role of Cymbopogon Citratus Extract in Protecting the Liver Against Injurious Effect of Dimethylnitrosamine in Rats. Int J Clin Dev Anat. 2016;1(4):89-95. doi: 10.11648/j.ijcda.20150104.12

Copy | Download

@article{10.11648/j.ijcda.20150104.12,
  author = {Naglaa Ali Saber Sarg and Usama Fouad Ahmed and Ali Mohamed Ali and Saleh Sayed Idris},
  title = {The Role of Cymbopogon Citratus Extract in Protecting the Liver Against Injurious Effect of Dimethylnitrosamine in Rats},
  journal = {International Journal of Clinical and Developmental Anatomy},
  volume = {1},
  number = {4},
  pages = {89-95},
  doi = {10.11648/j.ijcda.20150104.12},
  url = {https://doi.org/10.11648/j.ijcda.20150104.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijcda.20150104.12},
  abstract = {Background: N-Nitrosodimethylamine (NDMA) is a potent hepatotoxin that induces fibrosis of the liver. Cymbopogon citratus, a plant that is widely cultivated to be eaten either fresh with food or dried in tea or soft drink has been reported to possess a number of medicinal and aromatic properties. Aim of the work: This work is aimed to study the protective effect of Cymbopogon citratus ethanolic leaf extract against hepatic injury and fibrosis induced by repeated intermittent administration of Dimethylnitrosamine (DMN) in rats. Materials and methods: A total of 30 rats divided into 3 groups were used. Group 1 served as control, Group 2 was given intraperitoneal injection with DMA at a concentration of 10mg/kg body weight on three consecutive days of each week over a period of three weeks. Group 3 was given DMA at a concentration of 10mg/kg body weight on three consecutive days of each week over a period of three weeks and 100 mg/kg body weight of Cymbopogon citratus ethanolic aqueous leaf extract 5 hours after administration of DMN. Results: It was found that DMN produces histopathological changes of the liver; including signs of severe hepatic injury. The cytoplasm of most hepatocytes appeared vacuolated with pyknotic and karyolytic nuclei, congested blood vessels and focal necrotic areas with marked increase of the collagen fibers deposition around the blood vessels. Ultrastructural changes showed Pyknotic nuclei, vacuolated cytoplasm and reduction or complete loss of cristae in the mitochondria. These were reversed by simultaneous treatment with Cymbopogon citratus. In conclusion: The results of this present study indicated that aqueous leaf extracts of Cymbopogon citratus has an antihepatotoxic action against DMN induced hepatic oxidative damage in rats which might be ascribed to its antioxidant and free radical scavenging property.},
 year = {2016}
}

Copy | Download

TY  - JOUR
T1  - The Role of Cymbopogon Citratus Extract in Protecting the Liver Against Injurious Effect of Dimethylnitrosamine in Rats
AU  - Naglaa Ali Saber Sarg
AU  - Usama Fouad Ahmed
AU  - Ali Mohamed Ali
AU  - Saleh Sayed Idris
Y1  - 2016/02/26
PY  - 2016
N1  - https://doi.org/10.11648/j.ijcda.20150104.12
DO  - 10.11648/j.ijcda.20150104.12
T2  - International Journal of Clinical and Developmental Anatomy
JF  - International Journal of Clinical and Developmental Anatomy
JO  - International Journal of Clinical and Developmental Anatomy
SP  - 89
EP  - 95
PB  - Science Publishing Group
SN  - 2469-8008
UR  - https://doi.org/10.11648/j.ijcda.20150104.12
AB  - Background: N-Nitrosodimethylamine (NDMA) is a potent hepatotoxin that induces fibrosis of the liver. Cymbopogon citratus, a plant that is widely cultivated to be eaten either fresh with food or dried in tea or soft drink has been reported to possess a number of medicinal and aromatic properties. Aim of the work: This work is aimed to study the protective effect of Cymbopogon citratus ethanolic leaf extract against hepatic injury and fibrosis induced by repeated intermittent administration of Dimethylnitrosamine (DMN) in rats. Materials and methods: A total of 30 rats divided into 3 groups were used. Group 1 served as control, Group 2 was given intraperitoneal injection with DMA at a concentration of 10mg/kg body weight on three consecutive days of each week over a period of three weeks. Group 3 was given DMA at a concentration of 10mg/kg body weight on three consecutive days of each week over a period of three weeks and 100 mg/kg body weight of Cymbopogon citratus ethanolic aqueous leaf extract 5 hours after administration of DMN. Results: It was found that DMN produces histopathological changes of the liver; including signs of severe hepatic injury. The cytoplasm of most hepatocytes appeared vacuolated with pyknotic and karyolytic nuclei, congested blood vessels and focal necrotic areas with marked increase of the collagen fibers deposition around the blood vessels. Ultrastructural changes showed Pyknotic nuclei, vacuolated cytoplasm and reduction or complete loss of cristae in the mitochondria. These were reversed by simultaneous treatment with Cymbopogon citratus. In conclusion: The results of this present study indicated that aqueous leaf extracts of Cymbopogon citratus has an antihepatotoxic action against DMN induced hepatic oxidative damage in rats which might be ascribed to its antioxidant and free radical scavenging property.
VL  - 1
IS  - 4
ER  -

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