The Role of Morus Nigra Extract and Its Active Compounds as Drug Candidate on Human Colorectal Adenocarcinoma Cell Line HT-29

Ece Çakıroğlu; Tuğba Uysal; Gizem Çalıbaşı Koçal; Fatih Aygenli; Gülin Baran; Yasemin Baskın

doi:doi:10.11648/j.ijcocr.20170201.13

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The Role of Morus Nigra Extract and Its Active Compounds as Drug Candidate on Human Colorectal Adenocarcinoma Cell Line HT-29

Ece Çakıroğlu, Tuğba Uysal, Gizem Çalıbaşı Koçal, Fatih Aygenli, Gülin Baran, Yasemin Baskın

Published in International Journal of Clinical Oncology and Cancer Research (Volume 2, Issue 1)

Received: 15 January 2017 Accepted: 3 February 2017 Published: 1 March 2017

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Abstract

According to laboratory-based in vitro researches, there are numerous medicinal plants and natural compounds that indicate potential as an anticancer agent. Morus nigra (M.nigra) (black mulberry) and its active components are strong candidates to be anticancer agents. The purpose of the present study was to investigate antiproliferative and antimigratory effects of M.nigra extract, its leptin Morniga G (MorG) and one of its component Chalcone 4 hydrate; and their synergistic effect in combination with cetuximab application on colorectal adenocarcinoma cell line HT-29. The antiproliferative effect was determined by impedance based proliferation assay following exposure to M.nigra extract (10%, 1%, 0.1%), MorG (0.5, 5, 50 µM) and Chalcone 4 hydrate (0.5, 5, 50 µM) for 48 hours. The antimigratory effect of M.nigra extract and its coponents, was investigated by a wound-healing assay for 48 h. According to results, Morus nigra extract and its leptin MorG reduced cell viability. After 48 hours of 200 µg/ml Cetuximab exposure with M.nigra extract and MorG at different concentrations, a significant decrease on the cell viability was detected when compared to Cetuximab application. MorG can be suggested as a potantial conjugate for targeted drug. However, futher studies are required to fully understand its mechanisms of action.

Published in	International Journal of Clinical Oncology and Cancer Research (Volume 2, Issue 1)
DOI	10.11648/j.ijcocr.20170201.13
Page(s)	10-14
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2017. Published by Science Publishing Group

Keywords

Morus Nigra, Morniga G, Chalcone 4 Hydrate, Colorectal Adenocarcinoma, HT-29

References

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[5]	Singh T, Wu JH, Peumans WJ, Rouge P, Van Damme EJ, Wu AM (2007). Recognition profile of Morus nigra agglutinin (Morniga G) expressed by monomeric ligands, simple clusters and mammalian polyvalent glycotopes. Mol Immunol 44: 451–462.
[6]	Wei H, Zhu JJ, Liu XQ, Feng WH, Wang ZM, Yan LH (2016). Review of bioactive compounds from root barks of Morus plants (Sang-Bai-Pi) and their pharmacological effects. Cogent Chemistry 2: 1212320.
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[9]	Sigmaaldrich.com. (2016). Chalcone 4 hydrate ≥98% (HPLC) \| Sigma-Aldrich. [online] Available at: http://www.sigmaaldrich.com/catalog/product/sigma/c7870?lang=en&region=TR.
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[12]	Qadir MI, Ali M, Ibrahim Z (2014). Anticancer activity of Morus nigra leaves extract. Bangladesh J Pharmacol 9: 496-497.
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[14]	Takahashi M, Takara Ki Toyozato T, Wada K (2012). A novel bioactive chalcone of Morus australis inhibits tyrosinase activity and melanin biosynthesis in B16 melanoma cells. J Oleo Sci 61 (10): 5855-92.
[15]	Liu, B, Li, C. Y., Bian, H. J., Min, M. W., Chen, L. F., Bao, J. K (2009). Antiproliferative activity and apoptosis-inducing mechanism of Concanavalin A on human melanoma A375 cells. Arch. Biochem. Biophys 482: 1–6.

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Ece Çakıroğlu, Tuğba Uysal, Gizem Çalıbaşı Koçal, Fatih Aygenli, Gülin Baran, et al. (2017). The Role of Morus Nigra Extract and Its Active Compounds as Drug Candidate on Human Colorectal Adenocarcinoma Cell Line HT-29. International Journal of Clinical Oncology and Cancer Research, 2(1), 10-14. https://doi.org/10.11648/j.ijcocr.20170201.13

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ACS Style

Ece Çakıroğlu; Tuğba Uysal; Gizem Çalıbaşı Koçal; Fatih Aygenli; Gülin Baran, et al. The Role of Morus Nigra Extract and Its Active Compounds as Drug Candidate on Human Colorectal Adenocarcinoma Cell Line HT-29. Int. J. Clin. Oncol. Cancer Res. 2017, 2(1), 10-14. doi: 10.11648/j.ijcocr.20170201.13

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AMA Style

Ece Çakıroğlu, Tuğba Uysal, Gizem Çalıbaşı Koçal, Fatih Aygenli, Gülin Baran, et al. The Role of Morus Nigra Extract and Its Active Compounds as Drug Candidate on Human Colorectal Adenocarcinoma Cell Line HT-29. Int J Clin Oncol Cancer Res. 2017;2(1):10-14. doi: 10.11648/j.ijcocr.20170201.13

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@article{10.11648/j.ijcocr.20170201.13,
  author = {Ece Çakıroğlu and Tuğba Uysal and Gizem Çalıbaşı Koçal and Fatih Aygenli and Gülin Baran and Yasemin Baskın},
  title = {The Role of Morus Nigra Extract and Its Active Compounds as Drug Candidate on Human Colorectal Adenocarcinoma Cell Line HT-29},
  journal = {International Journal of Clinical Oncology and Cancer Research},
  volume = {2},
  number = {1},
  pages = {10-14},
  doi = {10.11648/j.ijcocr.20170201.13},
  url = {https://doi.org/10.11648/j.ijcocr.20170201.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijcocr.20170201.13},
  abstract = {According to laboratory-based in vitro researches, there are numerous medicinal plants and natural compounds that indicate potential as an anticancer agent. Morus nigra (M.nigra) (black mulberry) and its active components are strong candidates to be anticancer agents. The purpose of the present study was to investigate antiproliferative and antimigratory effects of M.nigra extract, its leptin Morniga G (MorG) and one of its component Chalcone 4 hydrate; and their synergistic effect in combination with cetuximab application on colorectal adenocarcinoma cell line HT-29. The antiproliferative effect was determined by impedance based proliferation assay following exposure to M.nigra extract (10%, 1%, 0.1%), MorG (0.5, 5, 50 µM) and Chalcone 4 hydrate (0.5, 5, 50 µM) for 48 hours. The antimigratory effect of M.nigra extract and its coponents, was investigated by a wound-healing assay for 48 h. According to results, Morus nigra extract and its leptin MorG reduced cell viability. After 48 hours of 200 µg/ml Cetuximab exposure with M.nigra extract and MorG at different concentrations, a significant decrease on the cell viability was detected when compared to Cetuximab application. MorG can be suggested as a potantial conjugate for targeted drug. However, futher studies are required to fully understand its mechanisms of action.},
 year = {2017}
}

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TY  - JOUR
T1  - The Role of Morus Nigra Extract and Its Active Compounds as Drug Candidate on Human Colorectal Adenocarcinoma Cell Line HT-29
AU  - Ece Çakıroğlu
AU  - Tuğba Uysal
AU  - Gizem Çalıbaşı Koçal
AU  - Fatih Aygenli
AU  - Gülin Baran
AU  - Yasemin Baskın
Y1  - 2017/03/01
PY  - 2017
N1  - https://doi.org/10.11648/j.ijcocr.20170201.13
DO  - 10.11648/j.ijcocr.20170201.13
T2  - International Journal of Clinical Oncology and Cancer Research
JF  - International Journal of Clinical Oncology and Cancer Research
JO  - International Journal of Clinical Oncology and Cancer Research
SP  - 10
EP  - 14
PB  - Science Publishing Group
SN  - 2578-9511
UR  - https://doi.org/10.11648/j.ijcocr.20170201.13
AB  - According to laboratory-based in vitro researches, there are numerous medicinal plants and natural compounds that indicate potential as an anticancer agent. Morus nigra (M.nigra) (black mulberry) and its active components are strong candidates to be anticancer agents. The purpose of the present study was to investigate antiproliferative and antimigratory effects of M.nigra extract, its leptin Morniga G (MorG) and one of its component Chalcone 4 hydrate; and their synergistic effect in combination with cetuximab application on colorectal adenocarcinoma cell line HT-29. The antiproliferative effect was determined by impedance based proliferation assay following exposure to M.nigra extract (10%, 1%, 0.1%), MorG (0.5, 5, 50 µM) and Chalcone 4 hydrate (0.5, 5, 50 µM) for 48 hours. The antimigratory effect of M.nigra extract and its coponents, was investigated by a wound-healing assay for 48 h. According to results, Morus nigra extract and its leptin MorG reduced cell viability. After 48 hours of 200 µg/ml Cetuximab exposure with M.nigra extract and MorG at different concentrations, a significant decrease on the cell viability was detected when compared to Cetuximab application. MorG can be suggested as a potantial conjugate for targeted drug. However, futher studies are required to fully understand its mechanisms of action.
VL  - 2
IS  - 1
ER  -

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Author Information

Ece Çakıroğlu
Tuğba Uysal
Gizem Çalıbaşı Koçal
Fatih Aygenli

Department of Genetics and Bioengineering, Faculty of Engineering, Yeditepe University, Istanbul, Turkey
Gülin Baran

Department of Genetics and Bioengineering, Faculty of Engineering, Yeditepe University, Istanbul, Turkey
Yasemin Baskın

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APA Style

Ece Çakıroğlu, Tuğba Uysal, Gizem Çalıbaşı Koçal, Fatih Aygenli, Gülin Baran, et al. (2017). The Role of Morus Nigra Extract and Its Active Compounds as Drug Candidate on Human Colorectal Adenocarcinoma Cell Line HT-29. International Journal of Clinical Oncology and Cancer Research, 2(1), 10-14. https://doi.org/10.11648/j.ijcocr.20170201.13

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ACS Style

Ece Çakıroğlu; Tuğba Uysal; Gizem Çalıbaşı Koçal; Fatih Aygenli; Gülin Baran, et al. The Role of Morus Nigra Extract and Its Active Compounds as Drug Candidate on Human Colorectal Adenocarcinoma Cell Line HT-29. Int. J. Clin. Oncol. Cancer Res. 2017, 2(1), 10-14. doi: 10.11648/j.ijcocr.20170201.13

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AMA Style

Ece Çakıroğlu, Tuğba Uysal, Gizem Çalıbaşı Koçal, Fatih Aygenli, Gülin Baran, et al. The Role of Morus Nigra Extract and Its Active Compounds as Drug Candidate on Human Colorectal Adenocarcinoma Cell Line HT-29. Int J Clin Oncol Cancer Res. 2017;2(1):10-14. doi: 10.11648/j.ijcocr.20170201.13

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@article{10.11648/j.ijcocr.20170201.13,
  author = {Ece Çakıroğlu and Tuğba Uysal and Gizem Çalıbaşı Koçal and Fatih Aygenli and Gülin Baran and Yasemin Baskın},
  title = {The Role of Morus Nigra Extract and Its Active Compounds as Drug Candidate on Human Colorectal Adenocarcinoma Cell Line HT-29},
  journal = {International Journal of Clinical Oncology and Cancer Research},
  volume = {2},
  number = {1},
  pages = {10-14},
  doi = {10.11648/j.ijcocr.20170201.13},
  url = {https://doi.org/10.11648/j.ijcocr.20170201.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijcocr.20170201.13},
  abstract = {According to laboratory-based in vitro researches, there are numerous medicinal plants and natural compounds that indicate potential as an anticancer agent. Morus nigra (M.nigra) (black mulberry) and its active components are strong candidates to be anticancer agents. The purpose of the present study was to investigate antiproliferative and antimigratory effects of M.nigra extract, its leptin Morniga G (MorG) and one of its component Chalcone 4 hydrate; and their synergistic effect in combination with cetuximab application on colorectal adenocarcinoma cell line HT-29. The antiproliferative effect was determined by impedance based proliferation assay following exposure to M.nigra extract (10%, 1%, 0.1%), MorG (0.5, 5, 50 µM) and Chalcone 4 hydrate (0.5, 5, 50 µM) for 48 hours. The antimigratory effect of M.nigra extract and its coponents, was investigated by a wound-healing assay for 48 h. According to results, Morus nigra extract and its leptin MorG reduced cell viability. After 48 hours of 200 µg/ml Cetuximab exposure with M.nigra extract and MorG at different concentrations, a significant decrease on the cell viability was detected when compared to Cetuximab application. MorG can be suggested as a potantial conjugate for targeted drug. However, futher studies are required to fully understand its mechanisms of action.},
 year = {2017}
}

Copy | Download

TY  - JOUR
T1  - The Role of Morus Nigra Extract and Its Active Compounds as Drug Candidate on Human Colorectal Adenocarcinoma Cell Line HT-29
AU  - Ece Çakıroğlu
AU  - Tuğba Uysal
AU  - Gizem Çalıbaşı Koçal
AU  - Fatih Aygenli
AU  - Gülin Baran
AU  - Yasemin Baskın
Y1  - 2017/03/01
PY  - 2017
N1  - https://doi.org/10.11648/j.ijcocr.20170201.13
DO  - 10.11648/j.ijcocr.20170201.13
T2  - International Journal of Clinical Oncology and Cancer Research
JF  - International Journal of Clinical Oncology and Cancer Research
JO  - International Journal of Clinical Oncology and Cancer Research
SP  - 10
EP  - 14
PB  - Science Publishing Group
SN  - 2578-9511
UR  - https://doi.org/10.11648/j.ijcocr.20170201.13
AB  - According to laboratory-based in vitro researches, there are numerous medicinal plants and natural compounds that indicate potential as an anticancer agent. Morus nigra (M.nigra) (black mulberry) and its active components are strong candidates to be anticancer agents. The purpose of the present study was to investigate antiproliferative and antimigratory effects of M.nigra extract, its leptin Morniga G (MorG) and one of its component Chalcone 4 hydrate; and their synergistic effect in combination with cetuximab application on colorectal adenocarcinoma cell line HT-29. The antiproliferative effect was determined by impedance based proliferation assay following exposure to M.nigra extract (10%, 1%, 0.1%), MorG (0.5, 5, 50 µM) and Chalcone 4 hydrate (0.5, 5, 50 µM) for 48 hours. The antimigratory effect of M.nigra extract and its coponents, was investigated by a wound-healing assay for 48 h. According to results, Morus nigra extract and its leptin MorG reduced cell viability. After 48 hours of 200 µg/ml Cetuximab exposure with M.nigra extract and MorG at different concentrations, a significant decrease on the cell viability was detected when compared to Cetuximab application. MorG can be suggested as a potantial conjugate for targeted drug. However, futher studies are required to fully understand its mechanisms of action.
VL  - 2
IS  - 1
ER  -

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