A Stereological Study of Key Histological Structures in the Kidneys of Rats from Young to Old Age

Dan Wang; Yang Guo; Yangyou Li; Xiaohong Wen; Zhengwei Yang

doi:doi:10.11648/j.ijcu.20210502.19

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A Stereological Study of Key Histological Structures in the Kidneys of Rats from Young to Old Age

Dan Wang, Yang Guo, Yangyou Li, Xiaohong Wen, Zhengwei Yang

Published in International Journal of Clinical Urology (Volume 5, Issue 2)

Received: 13 November 2021 Accepted: 29 November 2021 Published: 24 December 2021

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Abstract

Quantitative (morphometric) study of the total amounts (per organ) and mean sizes of histological structures in individuals from young to old are essential for the understanding of age-related organ changes; stereological techniques are essential, reliable tools to obtain the quantitative data. Stereological study of all renal components, especially renal tubules, was lacking in age-related kidney studies and few studies used rats older than 24 months of age for a stereological analysis of the aging kidney. In the present study isotropic uniform random renal sections (methacrylate-embedded) were obtained from male Sprague-Dawley rats (89 per age-group) randomly sampled from a single cohort of normal animals at the ages of 3, 6, 12, 24 and 36 months, respectively. The sections were measured using various stereological methods to estimate the total amounts (per kidney) or mean sizes of key renal structures. The results demonstrated that the volume of kidney and the total volume or length of renal tubules increased continually from 3 to 24 months of age and then plateaued between 24 and 36 months of age. The total volume of renal corpuscles, glomeruli, Bowman’s space or interstitial tissue and the mean volume of renal corpuscles or glomeruli increased continually from 3 to 24 months and further until 36 months of age. The total number of glomeruli remained essentially constant and the relative volume of the cortex or medulla and the relative length of different segments of the renal tubules remained basically stable throughout the ages. Less than 5% of the renal corpuscular or tubular profiles were apparently atrophied at 24 or 36 months of age. The age-related results suggested that the rat renal tissues continued to develop adaptively or work actively, from young to old, to maintain normal physiological functions and the aging change in the kidney was primarily a compensatory or hypertrophic histological change.

Published in	International Journal of Clinical Urology (Volume 5, Issue 2)
DOI	10.11648/j.ijcu.20210502.19
Page(s)	94-107
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Rats, Kidney, Renal Corpuscles, Renal Tubules, Development, Aging, Histology, Stereology

References

[1]	Zhou XJ, Rakheja D, Yu X, Saxena R, Vaziri ND, Silva FG. The aging kidney. Kidney Int 2008; 74: 710-720.
[2]	Weinstein JR, Anderson S. The aging kidney: physiological changes. Adv Chronic Kidney Dis 2010; 17: 302-307.
[3]	Yang H, Fogo AB. Cell senescence in the aging kidney. J Am SocNephrol 2010; 21: 1436-1439.
[4]	Kanasaki K, Kitada M, Koya D. Pathophysiology of the aging kidney and therapeutic interventions. Hypertens Res 2012; 35: 1121-1128.
[5]	Denic A, Glassock RJ, Rule AD. Structural and functional changes with the aging kidney. Adv Chronic Kidney Dis 2016; 23: 19-28.
[6]	Bridges CC, Zalups RK. The aging kidney and the nephrotoxic effects of mercury. J Toxicol Environ Health B Crit Rev 2017; 20: 55-80.
[7]	Gundersen HJ, Bendtsen TF, Korbo L, Marcussen N, Møller A, NielsenK, Nyengaard JR, Pakkenberg B, Sørensen FB, Vesterby A, West MJ. Some new, simple and efficient stereological methods and their use in pathological research and diagnosis. APMIS 1988; 96: 379-394.
[8]	Gundersen HJ, Bagger P, Bendtsen TF, Evans SM, Korbo L, Marcussen N, Møller A, Nielsen K, Nyengaard JR, Pakkenberg B, Sørensen FB, Vesterby A, West MJ. The new stereological tools: disector, fractionator, nucleator and point sampled intercepts and their use in pathological research and diagnosis. APMIS 1988: 96: 857-881.
[9]	Yang ZW. Essential Tools for Morphometric Studies of Biological Tissues: Practical Stereological Methods. 1st ed. Beijing: Science Press. 2012 [Book in Chinese].
[10]	Guo Y, Li JM, Xiang Y, Li YY, Huang J, Deng XZ, Yang ZW. Quantitative (stereological) study of the epididymis and seminal vesicle in the rat from young to old. Andrologia 2019; 51: e13247.
[11]	XuW, Guo Y, Xiang Y, Yang ZW. Is there section deformation resulting in differential change of nuclear numerical densities along the z axis of thick methacrylate or paraffin sections? Microsc Res Tech 2019; 82: 1575-1583.
[12]	Quinn R. Comparing rat's to human's age: how old is my rat in people years? Nutrition 2005; 21: 775-777.
[13]	AndreolloNA, Santos EF, Araújo MR, Lopes LR. Rat's age versus human's age: what is the relationship? Arq Bras Cir Dig 2012; 25: 49-51.
[14]	Mattfeldt T, Mall G, Gharehbaghi H, Möller P. Estimation of surface area and length with the orientator. J Microsc1990; 159: 301-317.
[15]	Wang D, Wen XH, Guo Y, Xiang Y, Yang ZW. Comparison of two stereological methods with counts and diameters of renal tubular profiles for estimating the length of renal tubules. Acta Anatomica Sinica 2020; 51: 967-975 [Article in Chinese].
[16]	Xiang Y, Yang ZW. Detachment of methacrylate-embedded sections from microscope slides can be prevented by heating on hotplate. J Histol Histopathol 2014; 1: 10.
[17]	Verlander JW. Urinary System. In: Dellmann’s Textbook of Veterinary Histology. 6th ed. Eurell A, Frappier BL (eds.). Oxford: Blackwell Publishing. 2006. pp212-232.
[18]	Kang J, Dai XS, Yu TB, Wen B, Yang ZW. Glycogen accumulation in renal tubules, a key morphological change in the diabetic rat kidney. Acta Diabetol 2005; 42: 110-116.
[19]	Yang ZW, Wreford NG, de Kretser DM. A quantitative study of spermatogenesis in the developing rat testis. Biol Reprod 1990; 43: 629-635.
[20]	Fogo AB, Kashgarian M. Diagnostic Atlas of Renal Pathology. 2nd ed. Philadelphia: Elsevier Saunders. 2012. pp2-50, 353-364.
[21]	Gray JE. Chronic progressive nephrosis in the albino rat. CRC Crit Rev Toxicol 1977; 5: 115-144.
[22]	Zou WZ. Pathology of Renal Biopsy. 4th ed. Beijing: Peking University Medical Press. 2017. pp41-67.
[23]	Owen RA, Heywood R. Age-related variations in renal structure and function in Sprague-Dawley rats. Toxicol Pathol 1986; 14: 158-167.
[24]	Sadeghinezhad J, Nyengaard JR. Cat kidney glomeruli and tubules evaluated by design-based stereology. Anat Rec (Hoboken) 2019; 302: 1846-1854.
[25]	Dodane V, Chevalier J, Bariety J, Pratz J, Corman B. Longitudinal study of solute excretion and glomerular ultrastructure in an experimental model of aging rats free of kidney disease. Lab Invest 1991; 64: 377-391.
[26]	Anderson S, Rennke HG, Zatz R. Glomerular adaptations with normal aging and with long-term converting enzyme inhibition in rats. Am J Physiol 1994; 267: F35-F43.
[27]	Baylis C. Age-dependent glomerular damage in the rat. Dissociation between glomerular injury and both glomerular hypertension and hypertrophy. Male gender as a primary risk factor. J Clin Invest 1994; 94: 1823-1829.
[28]	Sáez F, Reverte V, Salazar F, Castells MT, Llinás MT, Salazar FJ. Hypertension and sex differences in the age-related renal changes when cyclooxygenase-2 activity is reduced during nephrogenesis. Hypertension 2009; 53: 331-337.
[29]	Bolignano D, Mattace-Raso F, Sijbrands EJ, Zoccali C. The aging kidney revisited: a systematic review. Ageing Res Rev 2014; 14: 65-80.
[30]	Nyengaard JR, Bendtsen TF. Glomerular number and size in relation to age, kidney weight, and body surface in normal man. Anat Rec 1992; 232: 194-201.
[31]	Denic A, Lieske JC, Chakkera HA, Poggio ED, Alexander MP, Singh P, Kremers WK, Lerman LO, Rule AD. The substantial loss of nephrons in healthy human kidneys with aging. J Am Soc Nephrol 2017; 28: 313-320.
[32]	Gholamzadeh S, Zarenezhad M, Montazeri M, Zareikordshooli M, Sadeghi G, Malekpour A, Hoseni S, Bahrani M, Hajatmand R. Statistical analysis of organ morphometric parameters and weights in south iranian adult autopsies. Medicine (Baltimore) 2017; 96: e6447.
[33]	McDermott GF, Ingram A, Scholey J, Kirkland JL, Whiteside CI. Glomerular dysfunction in the aging Fischer 344 rat is associated with excessive growth and normal mesangial cell function. J Gerontol A Biol Sci Med Sci1996; 51: M80-M85.
[34]	Ding G, Franki N, Kapasi AA, Reddy K, Gibbons N, Singhal PC. Tubular cell senescence and expression of TGF-beta1 and p21 (WAF1/CIP1) in tubulointerstitial fibrosis of aging rats. ExpMolPathol 2001; 70: 43-53.
[35]	Cortes P, Zhao X, Dumler F, Tilley BC, Atherton J. Age-related changes in glomerular volume and hydroxyproline content in rat and human. J Am Soc Nephrol 1992; 2: 1716-1725.
[36]	Nyengaard JR. Number and dimensions of rat glomerular capillaries in normal development and after nephrectomy. Kidney Int 1993; 43: 1049-1057.
[37]	Fliser D, Franek E, Joest M, Block S, Mutschler E, Ritz E. Renal function in the elderly: impact of hypertension and cardiac function. Kidney Int 1997; 51: 1196-1204.
[38]	Goldstein RS, Tarloff JB, Hook JB. Age-related nephropathy in laboratory rats. FASEB J 1988; 2: 2241-2251.
[39]	Li YM, Steffes M, Donnelly T, Liu C, Fuh H, Basgen J, Bucala R, Vlassara H. Prevention of cardiovascular and renal pathology of aging by the advanced glycation inhibitor aminoguanidine. Proc Natl Acad Sci USA 1996; 93: 3902-3907.
[40]	McNamara BJ, Diouf B, Hughson MD, Hoy WE, Bertram JF. Associations between age, body size and nephron number with individual glomerular volumes in urban West African males. Nephrol Dial Transplant 2009; 24: 1500-1506.
[41]	Bertram JF, Hoy WE. Ageing: nephron loss in the ageing kidney  it's more than you think. Nat Rev Nephrol 2016; 12: 585-586.
[42]	Gundersen HJ. Stereological estimation of tubular length. J Microsc 2002; 207: 155-160.
[43]	Glassock RJ, Rule AD. The implications of anatomical and functional changes of the aging kidney: with an emphasis on the glomeruli. Kidney Int 2012; 82: 270-277.
[44]	Zhengwei Y, McLachlan RI, Bremner WJ, Wreford NG. Quantitative (stereological) study of the normal spermatogenesis in the adult monkey (Macaca fascicularis). J Androl 1997; 18: 681-687.

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APA Style

Dan Wang, Yang Guo, Yangyou Li, Xiaohong Wen, Zhengwei Yang. (2021). A Stereological Study of Key Histological Structures in the Kidneys of Rats from Young to Old Age. International Journal of Clinical Urology, 5(2), 94-107. https://doi.org/10.11648/j.ijcu.20210502.19

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ACS Style

Dan Wang; Yang Guo; Yangyou Li; Xiaohong Wen; Zhengwei Yang. A Stereological Study of Key Histological Structures in the Kidneys of Rats from Young to Old Age. Int. J. Clin. Urol. 2021, 5(2), 94-107. doi: 10.11648/j.ijcu.20210502.19

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AMA Style

Dan Wang, Yang Guo, Yangyou Li, Xiaohong Wen, Zhengwei Yang. A Stereological Study of Key Histological Structures in the Kidneys of Rats from Young to Old Age. Int J Clin Urol. 2021;5(2):94-107. doi: 10.11648/j.ijcu.20210502.19

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@article{10.11648/j.ijcu.20210502.19,
  author = {Dan Wang and Yang Guo and Yangyou Li and Xiaohong Wen and Zhengwei Yang},
  title = {A Stereological Study of Key Histological Structures in the Kidneys of Rats from Young to Old Age},
  journal = {International Journal of Clinical Urology},
  volume = {5},
  number = {2},
  pages = {94-107},
  doi = {10.11648/j.ijcu.20210502.19},
  url = {https://doi.org/10.11648/j.ijcu.20210502.19},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijcu.20210502.19},
  abstract = {Quantitative (morphometric) study of the total amounts (per organ) and mean sizes of histological structures in individuals from young to old are essential for the understanding of age-related organ changes; stereological techniques are essential, reliable tools to obtain the quantitative data. Stereological study of all renal components, especially renal tubules, was lacking in age-related kidney studies and few studies used rats older than 24 months of age for a stereological analysis of the aging kidney. In the present study isotropic uniform random renal sections (methacrylate-embedded) were obtained from male Sprague-Dawley rats (89 per age-group) randomly sampled from a single cohort of normal animals at the ages of 3, 6, 12, 24 and 36 months, respectively. The sections were measured using various stereological methods to estimate the total amounts (per kidney) or mean sizes of key renal structures. The results demonstrated that the volume of kidney and the total volume or length of renal tubules increased continually from 3 to 24 months of age and then plateaued between 24 and 36 months of age. The total volume of renal corpuscles, glomeruli, Bowman’s space or interstitial tissue and the mean volume of renal corpuscles or glomeruli increased continually from 3 to 24 months and further until 36 months of age. The total number of glomeruli remained essentially constant and the relative volume of the cortex or medulla and the relative length of different segments of the renal tubules remained basically stable throughout the ages. Less than 5% of the renal corpuscular or tubular profiles were apparently atrophied at 24 or 36 months of age. The age-related results suggested that the rat renal tissues continued to develop adaptively or work actively, from young to old, to maintain normal physiological functions and the aging change in the kidney was primarily a compensatory or hypertrophic histological change.},
 year = {2021}
}

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TY  - JOUR
T1  - A Stereological Study of Key Histological Structures in the Kidneys of Rats from Young to Old Age
AU  - Dan Wang
AU  - Yang Guo
AU  - Yangyou Li
AU  - Xiaohong Wen
AU  - Zhengwei Yang
Y1  - 2021/12/24
PY  - 2021
N1  - https://doi.org/10.11648/j.ijcu.20210502.19
DO  - 10.11648/j.ijcu.20210502.19
T2  - International Journal of Clinical Urology
JF  - International Journal of Clinical Urology
JO  - International Journal of Clinical Urology
SP  - 94
EP  - 107
PB  - Science Publishing Group
SN  - 2640-1355
UR  - https://doi.org/10.11648/j.ijcu.20210502.19
AB  - Quantitative (morphometric) study of the total amounts (per organ) and mean sizes of histological structures in individuals from young to old are essential for the understanding of age-related organ changes; stereological techniques are essential, reliable tools to obtain the quantitative data. Stereological study of all renal components, especially renal tubules, was lacking in age-related kidney studies and few studies used rats older than 24 months of age for a stereological analysis of the aging kidney. In the present study isotropic uniform random renal sections (methacrylate-embedded) were obtained from male Sprague-Dawley rats (89 per age-group) randomly sampled from a single cohort of normal animals at the ages of 3, 6, 12, 24 and 36 months, respectively. The sections were measured using various stereological methods to estimate the total amounts (per kidney) or mean sizes of key renal structures. The results demonstrated that the volume of kidney and the total volume or length of renal tubules increased continually from 3 to 24 months of age and then plateaued between 24 and 36 months of age. The total volume of renal corpuscles, glomeruli, Bowman’s space or interstitial tissue and the mean volume of renal corpuscles or glomeruli increased continually from 3 to 24 months and further until 36 months of age. The total number of glomeruli remained essentially constant and the relative volume of the cortex or medulla and the relative length of different segments of the renal tubules remained basically stable throughout the ages. Less than 5% of the renal corpuscular or tubular profiles were apparently atrophied at 24 or 36 months of age. The age-related results suggested that the rat renal tissues continued to develop adaptively or work actively, from young to old, to maintain normal physiological functions and the aging change in the kidney was primarily a compensatory or hypertrophic histological change.
VL  - 5
IS  - 2
ER  -

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Author Information

Dan Wang

Department of Histology and Embryology, North Sichuan Medical College, Nanchong, China
Yang Guo

Morphometric Research Laboratory, North Sichuan Medical College, Nanchong, China
Yangyou Li

Department of Experimental Zoology, North Sichuan Medical College, Nanchong, China
Xiaohong Wen

Department of Histology and Embryology, North Sichuan Medical College, Nanchong, China
Zhengwei Yang

Morphometric Research Laboratory, North Sichuan Medical College, Nanchong, China

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APA Style

Dan Wang, Yang Guo, Yangyou Li, Xiaohong Wen, Zhengwei Yang. (2021). A Stereological Study of Key Histological Structures in the Kidneys of Rats from Young to Old Age. International Journal of Clinical Urology, 5(2), 94-107. https://doi.org/10.11648/j.ijcu.20210502.19

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ACS Style

Dan Wang; Yang Guo; Yangyou Li; Xiaohong Wen; Zhengwei Yang. A Stereological Study of Key Histological Structures in the Kidneys of Rats from Young to Old Age. Int. J. Clin. Urol. 2021, 5(2), 94-107. doi: 10.11648/j.ijcu.20210502.19

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AMA Style

Dan Wang, Yang Guo, Yangyou Li, Xiaohong Wen, Zhengwei Yang. A Stereological Study of Key Histological Structures in the Kidneys of Rats from Young to Old Age. Int J Clin Urol. 2021;5(2):94-107. doi: 10.11648/j.ijcu.20210502.19

Copy | Download

@article{10.11648/j.ijcu.20210502.19,
  author = {Dan Wang and Yang Guo and Yangyou Li and Xiaohong Wen and Zhengwei Yang},
  title = {A Stereological Study of Key Histological Structures in the Kidneys of Rats from Young to Old Age},
  journal = {International Journal of Clinical Urology},
  volume = {5},
  number = {2},
  pages = {94-107},
  doi = {10.11648/j.ijcu.20210502.19},
  url = {https://doi.org/10.11648/j.ijcu.20210502.19},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijcu.20210502.19},
  abstract = {Quantitative (morphometric) study of the total amounts (per organ) and mean sizes of histological structures in individuals from young to old are essential for the understanding of age-related organ changes; stereological techniques are essential, reliable tools to obtain the quantitative data. Stereological study of all renal components, especially renal tubules, was lacking in age-related kidney studies and few studies used rats older than 24 months of age for a stereological analysis of the aging kidney. In the present study isotropic uniform random renal sections (methacrylate-embedded) were obtained from male Sprague-Dawley rats (89 per age-group) randomly sampled from a single cohort of normal animals at the ages of 3, 6, 12, 24 and 36 months, respectively. The sections were measured using various stereological methods to estimate the total amounts (per kidney) or mean sizes of key renal structures. The results demonstrated that the volume of kidney and the total volume or length of renal tubules increased continually from 3 to 24 months of age and then plateaued between 24 and 36 months of age. The total volume of renal corpuscles, glomeruli, Bowman’s space or interstitial tissue and the mean volume of renal corpuscles or glomeruli increased continually from 3 to 24 months and further until 36 months of age. The total number of glomeruli remained essentially constant and the relative volume of the cortex or medulla and the relative length of different segments of the renal tubules remained basically stable throughout the ages. Less than 5% of the renal corpuscular or tubular profiles were apparently atrophied at 24 or 36 months of age. The age-related results suggested that the rat renal tissues continued to develop adaptively or work actively, from young to old, to maintain normal physiological functions and the aging change in the kidney was primarily a compensatory or hypertrophic histological change.},
 year = {2021}
}

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TY  - JOUR
T1  - A Stereological Study of Key Histological Structures in the Kidneys of Rats from Young to Old Age
AU  - Dan Wang
AU  - Yang Guo
AU  - Yangyou Li
AU  - Xiaohong Wen
AU  - Zhengwei Yang
Y1  - 2021/12/24
PY  - 2021
N1  - https://doi.org/10.11648/j.ijcu.20210502.19
DO  - 10.11648/j.ijcu.20210502.19
T2  - International Journal of Clinical Urology
JF  - International Journal of Clinical Urology
JO  - International Journal of Clinical Urology
SP  - 94
EP  - 107
PB  - Science Publishing Group
SN  - 2640-1355
UR  - https://doi.org/10.11648/j.ijcu.20210502.19
AB  - Quantitative (morphometric) study of the total amounts (per organ) and mean sizes of histological structures in individuals from young to old are essential for the understanding of age-related organ changes; stereological techniques are essential, reliable tools to obtain the quantitative data. Stereological study of all renal components, especially renal tubules, was lacking in age-related kidney studies and few studies used rats older than 24 months of age for a stereological analysis of the aging kidney. In the present study isotropic uniform random renal sections (methacrylate-embedded) were obtained from male Sprague-Dawley rats (89 per age-group) randomly sampled from a single cohort of normal animals at the ages of 3, 6, 12, 24 and 36 months, respectively. The sections were measured using various stereological methods to estimate the total amounts (per kidney) or mean sizes of key renal structures. The results demonstrated that the volume of kidney and the total volume or length of renal tubules increased continually from 3 to 24 months of age and then plateaued between 24 and 36 months of age. The total volume of renal corpuscles, glomeruli, Bowman’s space or interstitial tissue and the mean volume of renal corpuscles or glomeruli increased continually from 3 to 24 months and further until 36 months of age. The total number of glomeruli remained essentially constant and the relative volume of the cortex or medulla and the relative length of different segments of the renal tubules remained basically stable throughout the ages. Less than 5% of the renal corpuscular or tubular profiles were apparently atrophied at 24 or 36 months of age. The age-related results suggested that the rat renal tissues continued to develop adaptively or work actively, from young to old, to maintain normal physiological functions and the aging change in the kidney was primarily a compensatory or hypertrophic histological change.
VL  - 5
IS  - 2
ER  -

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