Effects of Partial Saturation on Nitrogen Removal and Bacterial Community in Vertical-flow Constructed Wetlands

Khadija Kraiem; Hamadi Kallali; Rim Werheni Ammeri; Bessadok Salma; Naceur Jedidi

doi:doi:10.11648/j.ijec.20210502.14

| Peer-Reviewed

Effects of Partial Saturation on Nitrogen Removal and Bacterial Community in Vertical-flow Constructed Wetlands

Khadija Kraiem, Hamadi Kallali, Rim Werheni Ammeri, Bessadok Salma, Naceur Jedidi

Published in International Journal of Environmental Chemistry (Volume 5, Issue 2)

Received: 2 July 2021 Accepted: 27 July 2021 Published: 19 October 2021

Views: Downloads:

Download PDF

Share This Article

Twitter
Linked In
Facebook

Abstract

The laboratory-scale pilot of constructed wetlands has been in operation for six months; (1) an unsaturated vertical flow constructed wetland (UVF-CW), this system was used to represent the classic vertical constructed wetlands, (2) a saturated vertical flow constructed wetland (SVF-CW), to evaluate the effects of the saturated condition on nitrogen removal and composition of the microbial community. The results showed that the saturation condition positiveley influenced the removal efficiencies of the nitrogen, the aeverage removal rate of the total kjeldahl nitrogen increased from 56% in unsaturated vertical flow constructed wetland (UVF-CW) to 63% in saturated vertical flow constructed wetland (SVF-CW). In addition, the microbial communities also was affected by the saturation condition, the relative abundances of nitrifying bacterium in UVF-CW are 13.8% (Nitrosomonas), 7.2% (Nitrosospira), 18.1% (Nitrospira) and 15.3% (Nitrobacter). In contrast, in SVF-CW, Nitrosomonas, Nitrosospira, Nitrospira and Nitrobacter only accounted for 6.8%, 5.6%, 7.4% and 10.6% respectively. However, the saturation condition seemed to increase denitrifying bacterium more than three times, in unsaturated vertical flow constructed wetland, only Pseudomonas (6.5%) and Paracoccus (4.85%) were detected, but in saturated vertical flow constructed wetland (SVF-CW), the abundance of Pseudomonas (13.08%) and Paracoccus (9.74%) were increased, and three other groups of denitrifying bacteria were also detected as Zoogloea (3.32%), Thauera (5.41%) and Thiobacillus (3%), due to the low availability of oxygen, it seems to be beneficial to denitrifying bacteria.

Published in	International Journal of Environmental Chemistry (Volume 5, Issue 2)
DOI	10.11648/j.ijec.20210502.14
Page(s)	38-44
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2021. Published by Science Publishing Group

Keywords

Constructed Wetlands, Rural Wastewater, Saturated Bed, Nitrogen Transforming Bacteria

References

[1]	Stevens, C. J. (2019) Nitrogen in the environment. Science, 363, 578-580.
[2]	EPA, (1993) Process Design Manual for Nitrogen Control. US EPA, Washington, DC.
[3]	Keene, N. A., Reusser, S. R., Scarborough, M. J., Grooms, A. L., Seib, M., Santo Domingo, J & Noguera, D. R. (2017) Pilot plant demonstration of stable and efficient high rate biological nutrient removal with low dissolved oxygen conditions. Water Research, 121, 72–85.
[4]	Liu, H., Hu, Z., Zhang, J., Ngo, H. H., Guo, W., Liang, S., Fan, J., Lu, S. & Wu, H. (2016) Optimizations on supply and distribution of dissolved oxygen in constructed wetlands: A review. Bioresource Technology, 214, 797–805.
[5]	Zhang, N., Yang, Y., Huang, L., Xie, H & Hu, Z.(2019) Birnessite-coated sand filled vertical flow constructed wetlands improved nutrients removal in a cold climate. RSC Adv. 2019.
[6]	Álvarez, J. A., Ávila, C., Otter, P., Kilian, R., Rolletschek, I. D. M., Molle, P., Khalil, N., Ameršek, I., Mishra, V. K., Jorgensen, C., Garfi, A., Carvalho, P & Arias, C. A. (2017) Constructed wetlands and solar-driven disinfection technologies for sustainable wastewater treatment and reclamation in rural India: SWINGS project. Water Science and Technology, (5-6), 1474-1489.
[7]	Paing, J., Guilbert, A., Gagnon, V & Chazarenc, F. (2015) Effect of climate, wastewater composition, loading rates, system age and design on performances of French vertical flow constructed wetlands: A survey based on 169 full scale systems. Ecological engineering, 80, 46–52.
[8]	Wang, J., Wang, Y., Bai, J., Liu, Z., Song, X., Yan, D., Abiyu, A., Zhao, Z & Yan, D. High efficiency of inorganic nitrogen removal by integrating biofilm-electrodewith constructed wetland: autotrophic denitrifying bacteria analysis. Bioresource Technology, 227, 7–14.
[9]	Pelissari, C., Ávila, C., Trein, C. M., García, J., Dultra de Armas, R & Sezerino, P. H. (2017) Nitrogen transforming bacteria within a full-scale partially saturated vertical subsurface flow constructed wetland treating urban wastewater. Science of the Total Environment, 574, 390–399.
[10]	Platzer, C. (1999) Design recommendations for subsurface flow constructed wetlands for nitrification and denitrification. Water Science and Technology, 40, 257–263.
[11]	Vymazal, J. (2007) Removal of nutrients in various types of constructed wetlands. Water Science and Technology, 380, 48–65.
[12]	Saeed, T & Sun, G. (2011) Kinetic modelling of nitrogen and organics removal in vertical and horizontal flow wetlands. Water Research, 45, 3137-3152.
[13]	Lavrova, S & Koumanova, B. (2010) Influence of recirculation in a lab-scale vertical flow constructed wetland on the treatment efficiency of landfill leachate. Bioresource Technology, 101, 1756–1761.
[14]	Meng, P., Pei, H., Hu, W., Shao, Y., Li, Z. (2014) How to increase microbial degradation in constructed wetlands: influencing factors and improvement measures. Bioresource Technology, 157, 316–326.
[15]	Pelissari, C., Guivernau, M., Vinas, M., Garcia, J., Velasco, M., Souza, S. S., Sezerino, P. H & Avila, C. (2018) Effects of partially saturated conditions on the metabolically active microbiome and on nitrogen removal in vertical subsurface flow constructed wetlands. Water. Research, 141, 185–195.
[16]	Hu, Y., He, F., Ma, L., Zhang, Y & Wu, Z. (2016) Microbial nitrogen removal pathways in integrated vertical-flow constructed wetland systems. Bioresource Technology, 207, 339–345.
[17]	Wu, H., Fan, J., Zhang, J., Hao, H., Guo, W., Liang, S., Lv, J., Lu, S., Wu, W & Wu, S. (2016) Bioresource Technology Intensified organics and nitrogen removal in the intermittent-aerated constructed wetland using a novel sludge ceramsite as substrate. Bioresource Technology, 210, 101–107.
[18]	Li, H & Tao, W. (2017) Efficient ammonia removal in recirculating vertical flow constructed wetlands: complementary roles of anammox and denitrification in simultaneous nitritation, anammox and denitrification process. Chemical Engineering, 317, 972–979.
[19]	Torrijos, V., Ruiz, I & Soto, M. (2017) Effect of step-feeding on the performance of lab-scale columns simulating vertical flow-horizontal flow constructed wetlands. Environmental Science and Pollution Research, 24, 1–14.
[20]	Kim, B., Gautier, M., Prost-Boucle, S., Molle, P., Michel, P & Gourdon, R. (2014) Performance evaluation of partially saturated vertical-flow constructed wetland with trickling filter and chemical precipitation for domestic and winery wastewaters treatment. Ecological engineering, 71, 41–47.
[21]	Silveira, D. D., Belli Filho, P., Philippi, L. S., Kim, B & Molle, P. (2015) Influence of partial saturation on total nitrogen removal in a single-stage French constructed wetland treating raw domestic wastewater. Ecological Engineering, 77, 257–264.
[22]	Dong, Z & Sun, T. (2007) A potential new process for improving nitrogen removal in constructed wetlands-promoting coexistence of partial nitrification and ANAMMOX. Ecological Engineering, 31, 69–78.
[23]	Kim, B., Gautier, M., Palma, G. O., Molle, P & Michel, P., Gourdon, R. (2015a) Pilot-scale study of vertical flow constructed wetland combined with trickling filter and ferric chloride coagulation: influence of irregular operational conditions. Water Science and Technology, 71, 1088–1096.
[24]	Mayo, A. W & Bigambo, T. (2005) Nitrogen transformation in horizontal subsurface flow constructed wetlands I: model development. Physics and Chemistry of the Earth Parts A B C, 30, 658–667.
[25]	Foladori, P., Bruni, L & Tamburini, S. (2015) Bacteria viability and decay in water and soil of vertical subsurface flow constructed wetlands. Ecological Engineering, 82, 49–56.
[26]	Adrados, B., Sánchez, O., Arias, C. A., Becares, E., Garrido, L., Mas, J., Brix, H & Morató, J. (2014) Microbial communities from different types of natural wastewater treatment systems: vertical and horizontal flow constructed wetlands and biofilters. Water Research, 55, 304–312.
[27]	Calheiros, C. S. C., Duque, A. F., Moura, A., Henriques, I. S., Correia, A., Rangel, A. O. S. S & Castro, P. M. L. (2009) Substrate effect on bacterial communities from constructed wetlands planted with Typha latifolia treating industrial wastewater. Ecological Engineering, 35, 744–753.
[28]	Button, M., Nivala, J., Weber, K. P., Aubron, T & Müller, R. A. (2015) Microbial community metabolic function in subsurface flow constructed wetlands of different designs. Ecological Engineering, 80, 162–171.
[29]	Moore, J. C., de Vries, W., Lipp, M., Grifiths, J. C. & Abernethy, D. R. (2010) Total Protein Methods and Their Potential Utility to Reduce the Risk of Food Protein Adulteration. Comprehensive Reviews in Food Science and Food Safety, 9, 330–351.
[30]	APHA. (2012) Standard methods for the examination of water and wastewater, 597 22^th ed. American Public Health Association, Washington, DC, USA.
[31]	Zwirglmaier, K. (2005) Fluorescence in situ hybridisation (FISH) – the next generation. Mini review. FEMS. Microbiol. Lett. 2005, 246, 151–158.
[32]	Amann, R. (1995) In situ identification of microorganism by whole cell hybridization with rRNA-targeted nucleic acid probes. In: Akkerman, A. D. L., van Elsas, J. D., de Bruijn, F. J. (Eds.), Molecular Microbial Ecology Manual. Kluwer Academic Publishers, Dodrecht, The Netherlands. 1995, pp. 1–15 3.3.6.
[33]	Daims, H., Lücker, S & Wagner, M. (2006) Daime, a novel image analysis programfor microbial ecology and biofilm research. Environmental Microbiology, 8, 200–213.
[34]	Kotti, I. P., Gikas, G. D & Tsihrintzis, V. A. (2010) Effect of operational and design parameters on removal efficiency of pilot-scale FWS constructed wetlands and comparison with HSF systems. Ecological Engineering, 36, 862-875.
[35]	Chang, J., Wu, S., Dai, Y., Liang, W & Wu, Z. (2013) Nitrogen removal from nitrate-laden wastewater by integrated vertical-flow constructed wetland systems. Ecological Engineering, 58, 192–201.
[36]	Huang, J., Cai, W., Zhong, Q & Wang, S. (2013) Influence of temperature on micro-environment, plant eco-physiology and nitrogen removal effect in subsurface flow constructed wetland. Ecol Eng. 2013, 60, 242–248.
[37]	Saeed, T & Sun, G. (2012) A review on nitrogen and organics removal mechanisms in subsurface flow constructed wetlands: dependency on environmental parameters, operating conditions and supporting media. Journal of Environmental Management, 112, 429–448.
[38]	Huang, M., Wang, Z & Qi, R. (2017) Enhancement of the complete autotrophic nitrogen removal over nitrite process in a modified single-stage subsurface vertical flow constructed wetland: effect of saturated zone depth. Bioresource Technology, 233, 191–199.
[39]	Kraiem, K., Kallali, H., Wahab, M. A & Fra-Vazquez, A. (2019) Mosquera-Corral, A., Jedidi, N. Comparative study on pilots between ANAMMOX favored conditions in a partially saturated vertical flow constructed wetland and a hybrid system for rural wastewater treatment. Science of the Total Environment, 670, 644–653.
[40]	Del Toro, A., Tejeda, A & Zurita, F. (2019) Addition of corn cob in the free drainage zone of partially saturated vertical wetlands planted with I. sibirica for total nitrogen removal-a pilot-scale study. Water, 11, 2151–2116.
[41]	Kizito, S., Lv, T., Wu, S., Ajmal, Z., Luo, H & Dong, R. (2017) Treatment of anaerobic digested effluent in biochar-packed vertical flow constructed wetland columns: Role of media and tidal operation Science of the Total Environment, 592, 197-205.
[42]	Guan, W., Yin, M., He, T & Xie, S. (2015) Influence of substrate type on microbial community structure in vertical-flow constructed wetland treating polluted river water. Environmental Science and Pollution Research, 22, 16202–16209.
[43]	Wang, Y., Wang, J., Zhao, X., Song X & Gong, J. (2016) The inhibition and adaptability of four wetland plant species to high concentration of ammonia wastewater and nitrogen removal efficiency in constructed wetlands. Bioresource Technology, 202, 198–205.
[44]	Vymazal, J & Kröpfelová, L. (2015) Multistage hybrid constructed wetland for enhanced removal of nitrogen, Ecological Engineering, 84, 202-208.
[45]	Zhang, Y., Cheng, Y., Yang, C., Luo, W., Zeng, G & Lu, L. (2015) Performance of system consisting of vertical flow trickling filter and horizontal flow multi-soil-layering reactor for treatment of rural wastewater. Bioresource Technology, 193, 424–432.
[46]	Ahn, Y. H. (2006) Sustainable nitrogen elimination biotechnologies: a review. Process Biochemistry, 41, 1709–1721.
[47]	Richardson, D. J. (2000) Bacterial respiration: a flexibe process for a changing environment. Microbiology, 146, 551–571.

Cite This Article

Plain Text BibTeX RIS

APA Style

Khadija Kraiem, Hamadi Kallali, Rim Werheni Ammeri, Bessadok Salma, Naceur Jedidi. (2021). Effects of Partial Saturation on Nitrogen Removal and Bacterial Community in Vertical-flow Constructed Wetlands. International Journal of Environmental Chemistry, 5(2), 38-44. https://doi.org/10.11648/j.ijec.20210502.14

Copy | Download

ACS Style

Khadija Kraiem; Hamadi Kallali; Rim Werheni Ammeri; Bessadok Salma; Naceur Jedidi. Effects of Partial Saturation on Nitrogen Removal and Bacterial Community in Vertical-flow Constructed Wetlands. Int. J. Environ. Chem. 2021, 5(2), 38-44. doi: 10.11648/j.ijec.20210502.14

Copy | Download

AMA Style

Khadija Kraiem, Hamadi Kallali, Rim Werheni Ammeri, Bessadok Salma, Naceur Jedidi. Effects of Partial Saturation on Nitrogen Removal and Bacterial Community in Vertical-flow Constructed Wetlands. Int J Environ Chem. 2021;5(2):38-44. doi: 10.11648/j.ijec.20210502.14

Copy | Download

@article{10.11648/j.ijec.20210502.14,
  author = {Khadija Kraiem and Hamadi Kallali and Rim Werheni Ammeri and Bessadok Salma and Naceur Jedidi},
  title = {Effects of Partial Saturation on Nitrogen Removal and Bacterial Community in Vertical-flow Constructed Wetlands},
  journal = {International Journal of Environmental Chemistry},
  volume = {5},
  number = {2},
  pages = {38-44},
  doi = {10.11648/j.ijec.20210502.14},
  url = {https://doi.org/10.11648/j.ijec.20210502.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijec.20210502.14},
  abstract = {The laboratory-scale pilot of constructed wetlands has been in operation for six months; (1) an unsaturated vertical flow constructed wetland (UVF-CW), this system was used to represent the classic vertical constructed wetlands, (2) a saturated vertical flow constructed wetland (SVF-CW), to evaluate the effects of the saturated condition on nitrogen removal and composition of the microbial community. The results showed that the saturation condition positiveley influenced the removal efficiencies of the nitrogen, the aeverage removal rate of the total kjeldahl nitrogen increased from 56% in unsaturated vertical flow constructed wetland (UVF-CW) to 63% in saturated vertical flow constructed wetland (SVF-CW). In addition, the microbial communities also was affected by the saturation condition, the relative abundances of nitrifying bacterium in UVF-CW are 13.8% (Nitrosomonas), 7.2% (Nitrosospira), 18.1% (Nitrospira) and 15.3% (Nitrobacter). In contrast, in SVF-CW, Nitrosomonas, Nitrosospira, Nitrospira and Nitrobacter only accounted for 6.8%, 5.6%, 7.4% and 10.6% respectively. However, the saturation condition seemed to increase denitrifying bacterium more than three times, in unsaturated vertical flow constructed wetland, only Pseudomonas (6.5%) and Paracoccus (4.85%) were detected, but in saturated vertical flow constructed wetland (SVF-CW), the abundance of Pseudomonas (13.08%) and Paracoccus (9.74%) were increased, and three other groups of denitrifying bacteria were also detected as Zoogloea (3.32%), Thauera (5.41%) and Thiobacillus (3%), due to the low availability of oxygen, it seems to be beneficial to denitrifying bacteria.},
 year = {2021}
}

Copy | Download

TY  - JOUR
T1  - Effects of Partial Saturation on Nitrogen Removal and Bacterial Community in Vertical-flow Constructed Wetlands
AU  - Khadija Kraiem
AU  - Hamadi Kallali
AU  - Rim Werheni Ammeri
AU  - Bessadok Salma
AU  - Naceur Jedidi
Y1  - 2021/10/19
PY  - 2021
N1  - https://doi.org/10.11648/j.ijec.20210502.14
DO  - 10.11648/j.ijec.20210502.14
T2  - International Journal of Environmental Chemistry
JF  - International Journal of Environmental Chemistry
JO  - International Journal of Environmental Chemistry
SP  - 38
EP  - 44
PB  - Science Publishing Group
SN  - 2640-1460
UR  - https://doi.org/10.11648/j.ijec.20210502.14
AB  - The laboratory-scale pilot of constructed wetlands has been in operation for six months; (1) an unsaturated vertical flow constructed wetland (UVF-CW), this system was used to represent the classic vertical constructed wetlands, (2) a saturated vertical flow constructed wetland (SVF-CW), to evaluate the effects of the saturated condition on nitrogen removal and composition of the microbial community. The results showed that the saturation condition positiveley influenced the removal efficiencies of the nitrogen, the aeverage removal rate of the total kjeldahl nitrogen increased from 56% in unsaturated vertical flow constructed wetland (UVF-CW) to 63% in saturated vertical flow constructed wetland (SVF-CW). In addition, the microbial communities also was affected by the saturation condition, the relative abundances of nitrifying bacterium in UVF-CW are 13.8% (Nitrosomonas), 7.2% (Nitrosospira), 18.1% (Nitrospira) and 15.3% (Nitrobacter). In contrast, in SVF-CW, Nitrosomonas, Nitrosospira, Nitrospira and Nitrobacter only accounted for 6.8%, 5.6%, 7.4% and 10.6% respectively. However, the saturation condition seemed to increase denitrifying bacterium more than three times, in unsaturated vertical flow constructed wetland, only Pseudomonas (6.5%) and Paracoccus (4.85%) were detected, but in saturated vertical flow constructed wetland (SVF-CW), the abundance of Pseudomonas (13.08%) and Paracoccus (9.74%) were increased, and three other groups of denitrifying bacteria were also detected as Zoogloea (3.32%), Thauera (5.41%) and Thiobacillus (3%), due to the low availability of oxygen, it seems to be beneficial to denitrifying bacteria.
VL  - 5
IS  - 2
ER  -

Copy | Download

Author Information

Khadija Kraiem

Effluent Treatment and Valorization Laboratory, Water Research and Technology Center, Nabeul, Tunisia
Hamadi Kallali

Effluent Treatment and Valorization Laboratory, Water Research and Technology Center, Nabeul, Tunisia
Rim Werheni Ammeri

Effluent Treatment and Valorization Laboratory, Water Research and Technology Center, Nabeul, Tunisia
Bessadok Salma

Laboratory of Microorganisms and Active Biomolecules, Faculty of Sciences, University El Manar, Tunis, Tunisia
Naceur Jedidi

Effluent Treatment and Valorization Laboratory, Water Research and Technology Center, Nabeul, Tunisia

Download PDF

Sections

Plain Text BibTeX RIS

APA Style

Khadija Kraiem, Hamadi Kallali, Rim Werheni Ammeri, Bessadok Salma, Naceur Jedidi. (2021). Effects of Partial Saturation on Nitrogen Removal and Bacterial Community in Vertical-flow Constructed Wetlands. International Journal of Environmental Chemistry, 5(2), 38-44. https://doi.org/10.11648/j.ijec.20210502.14

Copy | Download

ACS Style

Khadija Kraiem; Hamadi Kallali; Rim Werheni Ammeri; Bessadok Salma; Naceur Jedidi. Effects of Partial Saturation on Nitrogen Removal and Bacterial Community in Vertical-flow Constructed Wetlands. Int. J. Environ. Chem. 2021, 5(2), 38-44. doi: 10.11648/j.ijec.20210502.14

Copy | Download

AMA Style

Khadija Kraiem, Hamadi Kallali, Rim Werheni Ammeri, Bessadok Salma, Naceur Jedidi. Effects of Partial Saturation on Nitrogen Removal and Bacterial Community in Vertical-flow Constructed Wetlands. Int J Environ Chem. 2021;5(2):38-44. doi: 10.11648/j.ijec.20210502.14

Copy | Download

@article{10.11648/j.ijec.20210502.14,
  author = {Khadija Kraiem and Hamadi Kallali and Rim Werheni Ammeri and Bessadok Salma and Naceur Jedidi},
  title = {Effects of Partial Saturation on Nitrogen Removal and Bacterial Community in Vertical-flow Constructed Wetlands},
  journal = {International Journal of Environmental Chemistry},
  volume = {5},
  number = {2},
  pages = {38-44},
  doi = {10.11648/j.ijec.20210502.14},
  url = {https://doi.org/10.11648/j.ijec.20210502.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijec.20210502.14},
  abstract = {The laboratory-scale pilot of constructed wetlands has been in operation for six months; (1) an unsaturated vertical flow constructed wetland (UVF-CW), this system was used to represent the classic vertical constructed wetlands, (2) a saturated vertical flow constructed wetland (SVF-CW), to evaluate the effects of the saturated condition on nitrogen removal and composition of the microbial community. The results showed that the saturation condition positiveley influenced the removal efficiencies of the nitrogen, the aeverage removal rate of the total kjeldahl nitrogen increased from 56% in unsaturated vertical flow constructed wetland (UVF-CW) to 63% in saturated vertical flow constructed wetland (SVF-CW). In addition, the microbial communities also was affected by the saturation condition, the relative abundances of nitrifying bacterium in UVF-CW are 13.8% (Nitrosomonas), 7.2% (Nitrosospira), 18.1% (Nitrospira) and 15.3% (Nitrobacter). In contrast, in SVF-CW, Nitrosomonas, Nitrosospira, Nitrospira and Nitrobacter only accounted for 6.8%, 5.6%, 7.4% and 10.6% respectively. However, the saturation condition seemed to increase denitrifying bacterium more than three times, in unsaturated vertical flow constructed wetland, only Pseudomonas (6.5%) and Paracoccus (4.85%) were detected, but in saturated vertical flow constructed wetland (SVF-CW), the abundance of Pseudomonas (13.08%) and Paracoccus (9.74%) were increased, and three other groups of denitrifying bacteria were also detected as Zoogloea (3.32%), Thauera (5.41%) and Thiobacillus (3%), due to the low availability of oxygen, it seems to be beneficial to denitrifying bacteria.},
 year = {2021}
}

Copy | Download

TY  - JOUR
T1  - Effects of Partial Saturation on Nitrogen Removal and Bacterial Community in Vertical-flow Constructed Wetlands
AU  - Khadija Kraiem
AU  - Hamadi Kallali
AU  - Rim Werheni Ammeri
AU  - Bessadok Salma
AU  - Naceur Jedidi
Y1  - 2021/10/19
PY  - 2021
N1  - https://doi.org/10.11648/j.ijec.20210502.14
DO  - 10.11648/j.ijec.20210502.14
T2  - International Journal of Environmental Chemistry
JF  - International Journal of Environmental Chemistry
JO  - International Journal of Environmental Chemistry
SP  - 38
EP  - 44
PB  - Science Publishing Group
SN  - 2640-1460
UR  - https://doi.org/10.11648/j.ijec.20210502.14
AB  - The laboratory-scale pilot of constructed wetlands has been in operation for six months; (1) an unsaturated vertical flow constructed wetland (UVF-CW), this system was used to represent the classic vertical constructed wetlands, (2) a saturated vertical flow constructed wetland (SVF-CW), to evaluate the effects of the saturated condition on nitrogen removal and composition of the microbial community. The results showed that the saturation condition positiveley influenced the removal efficiencies of the nitrogen, the aeverage removal rate of the total kjeldahl nitrogen increased from 56% in unsaturated vertical flow constructed wetland (UVF-CW) to 63% in saturated vertical flow constructed wetland (SVF-CW). In addition, the microbial communities also was affected by the saturation condition, the relative abundances of nitrifying bacterium in UVF-CW are 13.8% (Nitrosomonas), 7.2% (Nitrosospira), 18.1% (Nitrospira) and 15.3% (Nitrobacter). In contrast, in SVF-CW, Nitrosomonas, Nitrosospira, Nitrospira and Nitrobacter only accounted for 6.8%, 5.6%, 7.4% and 10.6% respectively. However, the saturation condition seemed to increase denitrifying bacterium more than three times, in unsaturated vertical flow constructed wetland, only Pseudomonas (6.5%) and Paracoccus (4.85%) were detected, but in saturated vertical flow constructed wetland (SVF-CW), the abundance of Pseudomonas (13.08%) and Paracoccus (9.74%) were increased, and three other groups of denitrifying bacteria were also detected as Zoogloea (3.32%), Thauera (5.41%) and Thiobacillus (3%), due to the low availability of oxygen, it seems to be beneficial to denitrifying bacteria.
VL  - 5
IS  - 2
ER  -

Copy | Download