Lipolytic Effect of Eumicrotremus orbis Water Extracts in Differentiated 3T3-L1 Adipocytes

Byoung-Mok Kim; Heang-Jeun Heo; Dong-Soo Kim; Young-Myung Kim; In-Hack Jeong; Young-Min Chi

doi:doi:10.11648/j.ijnfs.20150401.20

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Lipolytic Effect of Eumicrotremus orbis Water Extracts in Differentiated 3T3-L1 Adipocytes

Byoung-Mok Kim, Heang-Jeun Heo, Dong-Soo Kim, Young-Myung Kim, In-Hack Jeong, Young-Min Chi

Published in International Journal of Nutrition and Food Sciences (Volume 4, Issue 1)

Received: 19 January 2015 Accepted: 1 February 2015 Published: 9 February 2015

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Abstract

In this study, we investigated the effects of Eumicrotremus orbis water extracts (EOWE) on lipolysis in differentiatied 3T3-L1 adipocytes and its mechanism. It was observed that there was no cytotoxicity for adipocytes of EOWE. The content of glycerol released in cell culture medium increased dependent on sample concentration. In differentiated 3T3-L1 adipocytes treated EOWE, hormone sensitive lipase (HSL) and phosphorylated HSL (p-HSL) protein level increased by approximately 3.43 and 1.77 times as compared to the controls. But, adipose triglyceride lipase (ATGL) protein level did not effect as compared to the controls. Treated EOWE inhibited the protein expression of perilipin A. In other word, it is judged to be due to, a content of glycerol released in cell culture medium increased through not only increase of HSL, p-HSL and p-PKA protein level, but inhibit of perilipin A. Therefore, our results suggest that EOWE may induce lipoylsis by increasing the protein levels of HSL, p-HSL and p-PKA.

Published in	International Journal of Nutrition and Food Sciences (Volume 4, Issue 1)
DOI	10.11648/j.ijnfs.20150401.20
Page(s)	69-74
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2015. Published by Science Publishing Group

Keywords

Lipolytic Effect, Eumicrotremus Orbis, 3T3-L1 Adipocytes, Anti-Obesity, Marine Resource

References

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APA Style

Byoung-Mok Kim, Heang-Jeun Heo, Dong-Soo Kim, Young-Myung Kim, In-Hack Jeong, et al. (2015). Lipolytic Effect of Eumicrotremus orbis Water Extracts in Differentiated 3T3-L1 Adipocytes. International Journal of Nutrition and Food Sciences, 4(1), 69-74. https://doi.org/10.11648/j.ijnfs.20150401.20

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ACS Style

Byoung-Mok Kim; Heang-Jeun Heo; Dong-Soo Kim; Young-Myung Kim; In-Hack Jeong, et al. Lipolytic Effect of Eumicrotremus orbis Water Extracts in Differentiated 3T3-L1 Adipocytes. Int. J. Nutr. Food Sci. 2015, 4(1), 69-74. doi: 10.11648/j.ijnfs.20150401.20

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AMA Style

Byoung-Mok Kim, Heang-Jeun Heo, Dong-Soo Kim, Young-Myung Kim, In-Hack Jeong, et al. Lipolytic Effect of Eumicrotremus orbis Water Extracts in Differentiated 3T3-L1 Adipocytes. Int J Nutr Food Sci. 2015;4(1):69-74. doi: 10.11648/j.ijnfs.20150401.20

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@article{10.11648/j.ijnfs.20150401.20,
  author = {Byoung-Mok Kim and Heang-Jeun Heo and Dong-Soo Kim and Young-Myung Kim and In-Hack Jeong and Young-Min Chi},
  title = {Lipolytic Effect of Eumicrotremus orbis Water Extracts in Differentiated 3T3-L1 Adipocytes},
  journal = {International Journal of Nutrition and Food Sciences},
  volume = {4},
  number = {1},
  pages = {69-74},
  doi = {10.11648/j.ijnfs.20150401.20},
  url = {https://doi.org/10.11648/j.ijnfs.20150401.20},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijnfs.20150401.20},
  abstract = {In this study, we investigated the effects of Eumicrotremus orbis water extracts (EOWE) on lipolysis in differentiatied 3T3-L1 adipocytes and its mechanism. It was observed that there was no cytotoxicity for adipocytes of EOWE. The content of glycerol released in cell culture medium increased dependent on sample concentration. In differentiated 3T3-L1 adipocytes treated EOWE, hormone sensitive lipase (HSL) and phosphorylated HSL (p-HSL) protein level increased by approximately 3.43 and 1.77 times as compared to the controls. But, adipose triglyceride lipase (ATGL) protein level did not effect as compared to the controls. Treated EOWE inhibited the protein expression of perilipin A. In other word, it is judged to be due to, a content of glycerol released in cell culture medium increased through not only increase of HSL, p-HSL and p-PKA protein level, but inhibit of perilipin A. Therefore, our results suggest that EOWE may induce lipoylsis by increasing the protein levels of HSL, p-HSL and p-PKA.},
 year = {2015}
}

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TY  - JOUR
T1  - Lipolytic Effect of Eumicrotremus orbis Water Extracts in Differentiated 3T3-L1 Adipocytes
AU  - Byoung-Mok Kim
AU  - Heang-Jeun Heo
AU  - Dong-Soo Kim
AU  - Young-Myung Kim
AU  - In-Hack Jeong
AU  - Young-Min Chi
Y1  - 2015/02/09
PY  - 2015
N1  - https://doi.org/10.11648/j.ijnfs.20150401.20
DO  - 10.11648/j.ijnfs.20150401.20
T2  - International Journal of Nutrition and Food Sciences
JF  - International Journal of Nutrition and Food Sciences
JO  - International Journal of Nutrition and Food Sciences
SP  - 69
EP  - 74
PB  - Science Publishing Group
SN  - 2327-2716
UR  - https://doi.org/10.11648/j.ijnfs.20150401.20
AB  - In this study, we investigated the effects of Eumicrotremus orbis water extracts (EOWE) on lipolysis in differentiatied 3T3-L1 adipocytes and its mechanism. It was observed that there was no cytotoxicity for adipocytes of EOWE. The content of glycerol released in cell culture medium increased dependent on sample concentration. In differentiated 3T3-L1 adipocytes treated EOWE, hormone sensitive lipase (HSL) and phosphorylated HSL (p-HSL) protein level increased by approximately 3.43 and 1.77 times as compared to the controls. But, adipose triglyceride lipase (ATGL) protein level did not effect as compared to the controls. Treated EOWE inhibited the protein expression of perilipin A. In other word, it is judged to be due to, a content of glycerol released in cell culture medium increased through not only increase of HSL, p-HSL and p-PKA protein level, but inhibit of perilipin A. Therefore, our results suggest that EOWE may induce lipoylsis by increasing the protein levels of HSL, p-HSL and p-PKA.
VL  - 4
IS  - 1
ER  -

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Author Information

Byoung-Mok Kim

Division of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul, Korea
Heang-Jeun Heo

Division of Metabolism and Functionality Research, Korea Food Research Institute, Seongnam, Korea
Dong-Soo Kim

Jeonbuk Institute for Bioindustry, Jeonju, Korea
Young-Myung Kim

Gi-jang Mulsan Col., Busan, Korea
In-Hack Jeong

Division of Marine Food Bioscience & Technology, Gangneung National University, Gangneung, Korea
Young-Min Chi

Division of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul, Korea

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APA Style

Byoung-Mok Kim, Heang-Jeun Heo, Dong-Soo Kim, Young-Myung Kim, In-Hack Jeong, et al. (2015). Lipolytic Effect of Eumicrotremus orbis Water Extracts in Differentiated 3T3-L1 Adipocytes. International Journal of Nutrition and Food Sciences, 4(1), 69-74. https://doi.org/10.11648/j.ijnfs.20150401.20

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ACS Style

Byoung-Mok Kim; Heang-Jeun Heo; Dong-Soo Kim; Young-Myung Kim; In-Hack Jeong, et al. Lipolytic Effect of Eumicrotremus orbis Water Extracts in Differentiated 3T3-L1 Adipocytes. Int. J. Nutr. Food Sci. 2015, 4(1), 69-74. doi: 10.11648/j.ijnfs.20150401.20

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AMA Style

Byoung-Mok Kim, Heang-Jeun Heo, Dong-Soo Kim, Young-Myung Kim, In-Hack Jeong, et al. Lipolytic Effect of Eumicrotremus orbis Water Extracts in Differentiated 3T3-L1 Adipocytes. Int J Nutr Food Sci. 2015;4(1):69-74. doi: 10.11648/j.ijnfs.20150401.20

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@article{10.11648/j.ijnfs.20150401.20,
  author = {Byoung-Mok Kim and Heang-Jeun Heo and Dong-Soo Kim and Young-Myung Kim and In-Hack Jeong and Young-Min Chi},
  title = {Lipolytic Effect of Eumicrotremus orbis Water Extracts in Differentiated 3T3-L1 Adipocytes},
  journal = {International Journal of Nutrition and Food Sciences},
  volume = {4},
  number = {1},
  pages = {69-74},
  doi = {10.11648/j.ijnfs.20150401.20},
  url = {https://doi.org/10.11648/j.ijnfs.20150401.20},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijnfs.20150401.20},
  abstract = {In this study, we investigated the effects of Eumicrotremus orbis water extracts (EOWE) on lipolysis in differentiatied 3T3-L1 adipocytes and its mechanism. It was observed that there was no cytotoxicity for adipocytes of EOWE. The content of glycerol released in cell culture medium increased dependent on sample concentration. In differentiated 3T3-L1 adipocytes treated EOWE, hormone sensitive lipase (HSL) and phosphorylated HSL (p-HSL) protein level increased by approximately 3.43 and 1.77 times as compared to the controls. But, adipose triglyceride lipase (ATGL) protein level did not effect as compared to the controls. Treated EOWE inhibited the protein expression of perilipin A. In other word, it is judged to be due to, a content of glycerol released in cell culture medium increased through not only increase of HSL, p-HSL and p-PKA protein level, but inhibit of perilipin A. Therefore, our results suggest that EOWE may induce lipoylsis by increasing the protein levels of HSL, p-HSL and p-PKA.},
 year = {2015}
}

Copy | Download

TY  - JOUR
T1  - Lipolytic Effect of Eumicrotremus orbis Water Extracts in Differentiated 3T3-L1 Adipocytes
AU  - Byoung-Mok Kim
AU  - Heang-Jeun Heo
AU  - Dong-Soo Kim
AU  - Young-Myung Kim
AU  - In-Hack Jeong
AU  - Young-Min Chi
Y1  - 2015/02/09
PY  - 2015
N1  - https://doi.org/10.11648/j.ijnfs.20150401.20
DO  - 10.11648/j.ijnfs.20150401.20
T2  - International Journal of Nutrition and Food Sciences
JF  - International Journal of Nutrition and Food Sciences
JO  - International Journal of Nutrition and Food Sciences
SP  - 69
EP  - 74
PB  - Science Publishing Group
SN  - 2327-2716
UR  - https://doi.org/10.11648/j.ijnfs.20150401.20
AB  - In this study, we investigated the effects of Eumicrotremus orbis water extracts (EOWE) on lipolysis in differentiatied 3T3-L1 adipocytes and its mechanism. It was observed that there was no cytotoxicity for adipocytes of EOWE. The content of glycerol released in cell culture medium increased dependent on sample concentration. In differentiated 3T3-L1 adipocytes treated EOWE, hormone sensitive lipase (HSL) and phosphorylated HSL (p-HSL) protein level increased by approximately 3.43 and 1.77 times as compared to the controls. But, adipose triglyceride lipase (ATGL) protein level did not effect as compared to the controls. Treated EOWE inhibited the protein expression of perilipin A. In other word, it is judged to be due to, a content of glycerol released in cell culture medium increased through not only increase of HSL, p-HSL and p-PKA protein level, but inhibit of perilipin A. Therefore, our results suggest that EOWE may induce lipoylsis by increasing the protein levels of HSL, p-HSL and p-PKA.
VL  - 4
IS  - 1
ER  -

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