Comparative Study of Selenium-Enriched Conditions from <i>Ganoderma lucidum</i> and <i>Yeasts</i>

Liu Pei-Li; Wang Da-Yan; Hao Yong-Chun; Huang Shu-Ming

doi:doi:10.11648/j.ijnfs.20160502.15

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Comparative Study of Selenium-Enriched Conditions from Ganoderma lucidum and Yeasts

Liu Pei-Li, Wang Da-Yan, Hao Yong-Chun, Huang Shu-Ming

Published in International Journal of Nutrition and Food Sciences (Volume 5, Issue 2)

Received: 17 January 2016 Accepted: 28 January 2016 Published: 22 March 2016

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Abstract

This paper compared the conditions of selenium-enriched from Ganoderma lucidum and yeasts. The optimal conditions for shake flask were carried out by a single factor test and orthogonal test L₉(3⁴), giving a comparative study on the ability of biotransformation of inorganic selenium between Ganoderma lucidum and yeasts. The orthogonal experimental results showed that the optimal conditions for shake flask growth of Ganoderma lucidum were as bellow: culture medium final concentration of Na₂SeO₃ (Se⁴⁺) was 20mg/L, 15% inoculum, incubation time 42h, medium volume 60ml/250ml. The total content of selenium in fermentation of G. lucidum was 1540.34±19.21µg/g, and the growth capacity of mycelium of G. lucidum was 51.56±0.065g/L, respectively. The optimal conditions for shake flask cell growth of yeasts were as bellow: culture medium final concentration of Na₂SeO₃ (Se⁴⁺) was 25mg/L, 10% inoculum, incubation time 48h, medium volume 50ml/250ml. The total content of selenium in fermentation of yeasts was 2454.86±29.82µg/g, and the growth capacity of cell of yeasts was 6.44±0.023g/L, respectively.

Published in	International Journal of Nutrition and Food Sciences (Volume 5, Issue 2)
DOI	10.11648/j.ijnfs.20160502.15
Page(s)	124-128
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2016. Published by Science Publishing Group

Keywords

Ganoderma lucidum (Fr.) Karst, Saccharomyces Cerevisiae, Selenium-Enriched, Single-Factor Test, Orthogonal Experiment

References

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[2]	Margaret P R. The importance of selenium to human health [J]. Lancet, 2000, 356: 233-241.
[3]	He Li-dong. Spectrometry for determination of organo-Selenium in Selenium-enriched yeast [J]. Science and technology of food industry, 2000, 21(5): 67-68 (In Chinese).
[4]	Ei Bayoumy K. The protective role of Se on genetic damage and on cancer [J]. Mutat. Ras. 2001, 475, 123-139.
[5]	Wang Feng-qin. Development of Yeast Containing Abundant Se [J]. Liquor-making Science & Technology, 2004, 3: 34-35 (In Chinese).
[6]	Zheng Wang, Liyang Zhang, Tianwei Tan. High cell density fermentation of Saccharomyces cerevisiae GS2 for selenium-enriched yeast production. Korean J. Chem. Eng., 27(6), 2010: 1836-1840.
[7]	Klaudia Čobanová, Vladimír Petrovič, Martin Mellen, et al. Effects of Dietary Form of Selenium on Its Distribution in Eggs. Biol. Trace. Elem. Res., 2011, 144: 736-746.
[8]	Bente Gammelgaard, Matthew I., Jackson, et al. Surveying selenium speciation from soil to cell-forms and transformations. Anal. Bioanal. Chem., 2011, 399: 1743-1763.
[9]	Simone Pieniz, Robson Andreazza, Jamile Queiroz Pereira, et al. Production of Selenium-Enriched Biomass by Enterococcus durans. Biol. Trace. Elem. Res., 2013, 155: 447-454.
[10]	Sonia Agostinho Nido, Shituleni Andreas Shituleni, Berhe Mekonnen Mengistu1, et al. Effects of Selenium-Enriched Probiotics on Lipid Metabolism, Antioxidative Status, Histopathological Lesions, and Related Gene Expression in Mice Fed a High-Fat Diet. Biol. Trace. Elem. Res., 2015.
[11]	M. Assuncao, L. L. Martins, M. P. Mourato, et al. Effect of selenium on growth and antioxidant enzyme activities of wine related yeasts. World J. Microbiol. Biotechnol., 2015, 31: 1899-1906.
[12]	Marek Kieliszek, Stanisław Błażejak, Iwona Gientka, et al. Accumulation and metabolism of selenium by yeast cells. Appl. Microbiol. Biotechnol., 2015, 99: 5373-5382.
[13]	Sheena Fagan, Rebecca Owens, Patrick Ward, et al. Biochemical Comparison of Commercial Selenium Yeast Preparations. Biol. Trace. Elem. Res., 2015, 166: 245-259.

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APA Style

Liu Pei-Li, Wang Da-Yan, Hao Yong-Chun, Huang Shu-Ming. (2016). Comparative Study of Selenium-Enriched Conditions from Ganoderma lucidum and Yeasts. International Journal of Nutrition and Food Sciences, 5(2), 124-128. https://doi.org/10.11648/j.ijnfs.20160502.15

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ACS Style

Liu Pei-Li; Wang Da-Yan; Hao Yong-Chun; Huang Shu-Ming. Comparative Study of Selenium-Enriched Conditions from Ganoderma lucidum and Yeasts. Int. J. Nutr. Food Sci. 2016, 5(2), 124-128. doi: 10.11648/j.ijnfs.20160502.15

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AMA Style

Liu Pei-Li, Wang Da-Yan, Hao Yong-Chun, Huang Shu-Ming. Comparative Study of Selenium-Enriched Conditions from Ganoderma lucidum and Yeasts. Int J Nutr Food Sci. 2016;5(2):124-128. doi: 10.11648/j.ijnfs.20160502.15

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@article{10.11648/j.ijnfs.20160502.15,
  author = {Liu Pei-Li and Wang Da-Yan and Hao Yong-Chun and Huang Shu-Ming},
  title = {Comparative Study of Selenium-Enriched Conditions from Ganoderma lucidum and Yeasts},
  journal = {International Journal of Nutrition and Food Sciences},
  volume = {5},
  number = {2},
  pages = {124-128},
  doi = {10.11648/j.ijnfs.20160502.15},
  url = {https://doi.org/10.11648/j.ijnfs.20160502.15},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijnfs.20160502.15},
  abstract = {This paper compared the conditions of selenium-enriched from Ganoderma lucidum and yeasts. The optimal conditions for shake flask were carried out by a single factor test and orthogonal test L9(34), giving a comparative study on the ability of biotransformation of inorganic selenium between Ganoderma lucidum and yeasts. The orthogonal experimental results showed that the optimal conditions for shake flask growth of Ganoderma lucidum were as bellow: culture medium final concentration of Na2SeO3 (Se4+) was 20mg/L, 15% inoculum, incubation time 42h, medium volume 60ml/250ml. The total content of selenium in fermentation of G. lucidum was 1540.34±19.21µg/g, and the growth capacity of mycelium of G. lucidum was 51.56±0.065g/L, respectively. The optimal conditions for shake flask cell growth of yeasts were as bellow: culture medium final concentration of Na2SeO3 (Se4+) was 25mg/L, 10% inoculum, incubation time 48h, medium volume 50ml/250ml. The total content of selenium in fermentation of yeasts was 2454.86±29.82µg/g, and the growth capacity of cell of yeasts was 6.44±0.023g/L, respectively.},
 year = {2016}
}

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TY  - JOUR
T1  - Comparative Study of Selenium-Enriched Conditions from Ganoderma lucidum and Yeasts
AU  - Liu Pei-Li
AU  - Wang Da-Yan
AU  - Hao Yong-Chun
AU  - Huang Shu-Ming
Y1  - 2016/03/22
PY  - 2016
N1  - https://doi.org/10.11648/j.ijnfs.20160502.15
DO  - 10.11648/j.ijnfs.20160502.15
T2  - International Journal of Nutrition and Food Sciences
JF  - International Journal of Nutrition and Food Sciences
JO  - International Journal of Nutrition and Food Sciences
SP  - 124
EP  - 128
PB  - Science Publishing Group
SN  - 2327-2716
UR  - https://doi.org/10.11648/j.ijnfs.20160502.15
AB  - This paper compared the conditions of selenium-enriched from Ganoderma lucidum and yeasts. The optimal conditions for shake flask were carried out by a single factor test and orthogonal test L9(34), giving a comparative study on the ability of biotransformation of inorganic selenium between Ganoderma lucidum and yeasts. The orthogonal experimental results showed that the optimal conditions for shake flask growth of Ganoderma lucidum were as bellow: culture medium final concentration of Na2SeO3 (Se4+) was 20mg/L, 15% inoculum, incubation time 42h, medium volume 60ml/250ml. The total content of selenium in fermentation of G. lucidum was 1540.34±19.21µg/g, and the growth capacity of mycelium of G. lucidum was 51.56±0.065g/L, respectively. The optimal conditions for shake flask cell growth of yeasts were as bellow: culture medium final concentration of Na2SeO3 (Se4+) was 25mg/L, 10% inoculum, incubation time 48h, medium volume 50ml/250ml. The total content of selenium in fermentation of yeasts was 2454.86±29.82µg/g, and the growth capacity of cell of yeasts was 6.44±0.023g/L, respectively.
VL  - 5
IS  - 2
ER  -

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Author Information

Liu Pei-Li

Department of Chemical and Materials Engineering, Hefei University, Hefei, Anhui, China
Wang Da-Yan

Anhui Star New Materials Technology Co., Ltd., Hefei, Anhui, China
Hao Yong-Chun

Department of Biological and Environmental Engineering, Hefei University, Hefei, Anhui, China
Huang Shu-Ming

Department of Biological and Environmental Engineering, Hefei University, Hefei, Anhui, China

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Plain Text BibTeX RIS

APA Style

Liu Pei-Li, Wang Da-Yan, Hao Yong-Chun, Huang Shu-Ming. (2016). Comparative Study of Selenium-Enriched Conditions from Ganoderma lucidum and Yeasts. International Journal of Nutrition and Food Sciences, 5(2), 124-128. https://doi.org/10.11648/j.ijnfs.20160502.15

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ACS Style

Liu Pei-Li; Wang Da-Yan; Hao Yong-Chun; Huang Shu-Ming. Comparative Study of Selenium-Enriched Conditions from Ganoderma lucidum and Yeasts. Int. J. Nutr. Food Sci. 2016, 5(2), 124-128. doi: 10.11648/j.ijnfs.20160502.15

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AMA Style

Liu Pei-Li, Wang Da-Yan, Hao Yong-Chun, Huang Shu-Ming. Comparative Study of Selenium-Enriched Conditions from Ganoderma lucidum and Yeasts. Int J Nutr Food Sci. 2016;5(2):124-128. doi: 10.11648/j.ijnfs.20160502.15

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@article{10.11648/j.ijnfs.20160502.15,
  author = {Liu Pei-Li and Wang Da-Yan and Hao Yong-Chun and Huang Shu-Ming},
  title = {Comparative Study of Selenium-Enriched Conditions from Ganoderma lucidum and Yeasts},
  journal = {International Journal of Nutrition and Food Sciences},
  volume = {5},
  number = {2},
  pages = {124-128},
  doi = {10.11648/j.ijnfs.20160502.15},
  url = {https://doi.org/10.11648/j.ijnfs.20160502.15},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijnfs.20160502.15},
  abstract = {This paper compared the conditions of selenium-enriched from Ganoderma lucidum and yeasts. The optimal conditions for shake flask were carried out by a single factor test and orthogonal test L9(34), giving a comparative study on the ability of biotransformation of inorganic selenium between Ganoderma lucidum and yeasts. The orthogonal experimental results showed that the optimal conditions for shake flask growth of Ganoderma lucidum were as bellow: culture medium final concentration of Na2SeO3 (Se4+) was 20mg/L, 15% inoculum, incubation time 42h, medium volume 60ml/250ml. The total content of selenium in fermentation of G. lucidum was 1540.34±19.21µg/g, and the growth capacity of mycelium of G. lucidum was 51.56±0.065g/L, respectively. The optimal conditions for shake flask cell growth of yeasts were as bellow: culture medium final concentration of Na2SeO3 (Se4+) was 25mg/L, 10% inoculum, incubation time 48h, medium volume 50ml/250ml. The total content of selenium in fermentation of yeasts was 2454.86±29.82µg/g, and the growth capacity of cell of yeasts was 6.44±0.023g/L, respectively.},
 year = {2016}
}

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TY  - JOUR
T1  - Comparative Study of Selenium-Enriched Conditions from Ganoderma lucidum and Yeasts
AU  - Liu Pei-Li
AU  - Wang Da-Yan
AU  - Hao Yong-Chun
AU  - Huang Shu-Ming
Y1  - 2016/03/22
PY  - 2016
N1  - https://doi.org/10.11648/j.ijnfs.20160502.15
DO  - 10.11648/j.ijnfs.20160502.15
T2  - International Journal of Nutrition and Food Sciences
JF  - International Journal of Nutrition and Food Sciences
JO  - International Journal of Nutrition and Food Sciences
SP  - 124
EP  - 128
PB  - Science Publishing Group
SN  - 2327-2716
UR  - https://doi.org/10.11648/j.ijnfs.20160502.15
AB  - This paper compared the conditions of selenium-enriched from Ganoderma lucidum and yeasts. The optimal conditions for shake flask were carried out by a single factor test and orthogonal test L9(34), giving a comparative study on the ability of biotransformation of inorganic selenium between Ganoderma lucidum and yeasts. The orthogonal experimental results showed that the optimal conditions for shake flask growth of Ganoderma lucidum were as bellow: culture medium final concentration of Na2SeO3 (Se4+) was 20mg/L, 15% inoculum, incubation time 42h, medium volume 60ml/250ml. The total content of selenium in fermentation of G. lucidum was 1540.34±19.21µg/g, and the growth capacity of mycelium of G. lucidum was 51.56±0.065g/L, respectively. The optimal conditions for shake flask cell growth of yeasts were as bellow: culture medium final concentration of Na2SeO3 (Se4+) was 25mg/L, 10% inoculum, incubation time 48h, medium volume 50ml/250ml. The total content of selenium in fermentation of yeasts was 2454.86±29.82µg/g, and the growth capacity of cell of yeasts was 6.44±0.023g/L, respectively.
VL  - 5
IS  - 2
ER  -

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