The Impact of Enzymes in the Hepatic Function

Alma Daja; Entela Treska

doi:doi:10.11648/j.ijsqa.20150101.12

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The Impact of Enzymes in the Hepatic Function

Alma Daja, Entela Treska

Published in International Journal of Science and Qualitative Analysis (Volume 1, Issue 1)

Received: 22 May 2015 Accepted: 27 May 2015 Published: 28 May 2015

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Abstract

The aim of this study is the application of Alkaline Phosphatase (ALP) and Gamma-glutamyl Transferase (GGT) activity, by using the corresponding kinetic methods, at the Clinical Chemistry Laboratories in our place. Enzymes are proteins synthesized in the cells of living organisms in order to play the role of bio-catalysts. Today are recognized about 2000 different enzymes. Some of those have been isolated in pure form of the chemical. We used serum as a material and we took into consideration 15 healthy patients, 15 patients who had viral hepatitis and 15 patients with idiopathic hyper-bilirubinemia. Solution where the substance is being analyzed, was treated with specific reagents according to the procedures such as incubation, heating, etc, until the color is formed. The absorbance of the coloring solution was measured using photometry at a wavelength of 405nm. As an analytical method for the determination of ALP, we chose 4-nitrophenylphosphate (4NPP) as a substrate, while as a buffer for the optimal pH adjustment of the reaction we used 2-amino-2 methyl -1-propanol (AMP). ALP was within permitted values from 100 to 290 U /l, whereas Gamma-glutamyl Transferase was at normal reference values of 5 to 51 U /l. Automated method was equal to the standard method, with a correlation coefficient of r = 0997. Accuracy within the series of measurements had a variation coefficient of only 1.25%. From the results taken from the laboratories, we had an increased precision, accuracy, sensitivity and reliability as a result of the technological process of electronic engineering of new modern techniques with monoclonal antibodies and immobilized enzymes.

Published in	International Journal of Science and Qualitative Analysis (Volume 1, Issue 1)
DOI	10.11648/j.ijsqa.20150101.12
Page(s)	6-10
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2015. Published by Science Publishing Group

Keywords

Clinical Chemistry, ALP Determination, GGT Assay Kit, Enzyme Classification, Hepatic Enzymes, Photometry

References

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[7]	Stryer L, Berg JM, Tymoczko JL (2002).Biochemistry (5th ed.). San Francisco: W.H. Freeman. ISBN 0-7167-4955-6.
[8]	Schomburg I, Chang A, Placzek S, Söhngen C, Rother M, Lang M et al. (Jan 2013). "BRENDA in 2013: integrated reactions, kinetic data, enzyme function data, improved disease classification: new options and contents in BRENDA". Nucleic Acids Research 41 (Database issue): D764–72. doi: 10.1093/ nar/gks 1049. PMID 23203881.
[9]	Radzicka A, Wolfenden R (Jan 1995). "A proficient enzyme". Science 267 (5194): 90–931. doi:10.1126/science.7809611. PMID 7809611.
[10]	Callahan BP, Miller BG (Dec 2007). "OMP decarboxylase--An enigma persists".Bioorganic Chemistry 35 (6): 465–9. doi: 10.1016/j.bioorg. 2007.07.004 PMID 17889251.
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Alma Daja, Entela Treska. (2015). The Impact of Enzymes in the Hepatic Function. International Journal of Science and Qualitative Analysis, 1(1), 6-10. https://doi.org/10.11648/j.ijsqa.20150101.12

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ACS Style

Alma Daja; Entela Treska. The Impact of Enzymes in the Hepatic Function. Int. J. Sci. Qual. Anal. 2015, 1(1), 6-10. doi: 10.11648/j.ijsqa.20150101.12

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Alma Daja, Entela Treska. The Impact of Enzymes in the Hepatic Function. Int J Sci Qual Anal. 2015;1(1):6-10. doi: 10.11648/j.ijsqa.20150101.12

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@article{10.11648/j.ijsqa.20150101.12,
  author = {Alma Daja and Entela Treska},
  title = {The Impact of Enzymes in the Hepatic Function},
  journal = {International Journal of Science and Qualitative Analysis},
  volume = {1},
  number = {1},
  pages = {6-10},
  doi = {10.11648/j.ijsqa.20150101.12},
  url = {https://doi.org/10.11648/j.ijsqa.20150101.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijsqa.20150101.12},
  abstract = {The aim of this study is the application of Alkaline Phosphatase (ALP) and Gamma-glutamyl Transferase (GGT) activity, by using the corresponding kinetic methods, at the Clinical Chemistry Laboratories in our place. Enzymes are proteins synthesized in the cells of living organisms in order to play the role of bio-catalysts. Today are recognized about 2000 different enzymes. Some of those have been isolated in pure form of the chemical. We used serum as a material and we took into consideration 15 healthy patients, 15 patients who had viral hepatitis and 15 patients with idiopathic hyper-bilirubinemia. Solution where the substance is being analyzed, was treated with specific reagents according to the procedures such as incubation, heating, etc, until the color is formed. The absorbance of the coloring solution was measured using photometry at a wavelength of 405nm. As an analytical method for the determination of ALP, we chose 4-nitrophenylphosphate (4NPP) as a substrate, while as a buffer for the optimal pH adjustment of the reaction we used 2-amino-2 methyl -1-propanol (AMP). ALP was within permitted values from 100 to 290 U /l, whereas Gamma-glutamyl Transferase was at normal reference values of 5 to 51 U /l. Automated method was equal to the standard method, with a correlation coefficient of r = 0997. Accuracy within the series of measurements had a variation coefficient of only 1.25%. From the results taken from the laboratories, we had an increased precision, accuracy, sensitivity and reliability as a result of the technological process of electronic engineering of new modern techniques with monoclonal antibodies and immobilized enzymes.},
 year = {2015}
}

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TY - JOUR
T1 - The Impact of Enzymes in the Hepatic Function
AU - Alma Daja
AU - Entela Treska
Y1 - 2015/05/28
PY - 2015
N1 - https://doi.org/10.11648/j.ijsqa.20150101.12
DO - 10.11648/j.ijsqa.20150101.12
T2 - International Journal of Science and Qualitative Analysis
JF - International Journal of Science and Qualitative Analysis
JO - International Journal of Science and Qualitative Analysis
SP - 6
EP - 10
PB - Science Publishing Group
SN - 2469-8164
UR - https://doi.org/10.11648/j.ijsqa.20150101.12
AB - The aim of this study is the application of Alkaline Phosphatase (ALP) and Gamma-glutamyl Transferase (GGT) activity, by using the corresponding kinetic methods, at the Clinical Chemistry Laboratories in our place. Enzymes are proteins synthesized in the cells of living organisms in order to play the role of bio-catalysts. Today are recognized about 2000 different enzymes. Some of those have been isolated in pure form of the chemical. We used serum as a material and we took into consideration 15 healthy patients, 15 patients who had viral hepatitis and 15 patients with idiopathic hyper-bilirubinemia. Solution where the substance is being analyzed, was treated with specific reagents according to the procedures such as incubation, heating, etc, until the color is formed. The absorbance of the coloring solution was measured using photometry at a wavelength of 405nm. As an analytical method for the determination of ALP, we chose 4-nitrophenylphosphate (4NPP) as a substrate, while as a buffer for the optimal pH adjustment of the reaction we used 2-amino-2 methyl -1-propanol (AMP). ALP was within permitted values from 100 to 290 U /l, whereas Gamma-glutamyl Transferase was at normal reference values of 5 to 51 U /l. Automated method was equal to the standard method, with a correlation coefficient of r = 0997. Accuracy within the series of measurements had a variation coefficient of only 1.25%. From the results taken from the laboratories, we had an increased precision, accuracy, sensitivity and reliability as a result of the technological process of electronic engineering of new modern techniques with monoclonal antibodies and immobilized enzymes.
VL - 1
IS - 1
ER -

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Author Information

Alma Daja

Clinical and Biochemical Laboratory, Health Centre nr 2, Tirana, Albania
Entela Treska

University Hospital of Obstetrics and Gynaecology “Queen Geraldine”, Tirana, Albania

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APA Style

Alma Daja, Entela Treska. (2015). The Impact of Enzymes in the Hepatic Function. International Journal of Science and Qualitative Analysis, 1(1), 6-10. https://doi.org/10.11648/j.ijsqa.20150101.12

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ACS Style

Alma Daja; Entela Treska. The Impact of Enzymes in the Hepatic Function. Int. J. Sci. Qual. Anal. 2015, 1(1), 6-10. doi: 10.11648/j.ijsqa.20150101.12

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AMA Style

Alma Daja, Entela Treska. The Impact of Enzymes in the Hepatic Function. Int J Sci Qual Anal. 2015;1(1):6-10. doi: 10.11648/j.ijsqa.20150101.12

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@article{10.11648/j.ijsqa.20150101.12,
  author = {Alma Daja and Entela Treska},
  title = {The Impact of Enzymes in the Hepatic Function},
  journal = {International Journal of Science and Qualitative Analysis},
  volume = {1},
  number = {1},
  pages = {6-10},
  doi = {10.11648/j.ijsqa.20150101.12},
  url = {https://doi.org/10.11648/j.ijsqa.20150101.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijsqa.20150101.12},
  abstract = {The aim of this study is the application of Alkaline Phosphatase (ALP) and Gamma-glutamyl Transferase (GGT) activity, by using the corresponding kinetic methods, at the Clinical Chemistry Laboratories in our place. Enzymes are proteins synthesized in the cells of living organisms in order to play the role of bio-catalysts. Today are recognized about 2000 different enzymes. Some of those have been isolated in pure form of the chemical. We used serum as a material and we took into consideration 15 healthy patients, 15 patients who had viral hepatitis and 15 patients with idiopathic hyper-bilirubinemia. Solution where the substance is being analyzed, was treated with specific reagents according to the procedures such as incubation, heating, etc, until the color is formed. The absorbance of the coloring solution was measured using photometry at a wavelength of 405nm. As an analytical method for the determination of ALP, we chose 4-nitrophenylphosphate (4NPP) as a substrate, while as a buffer for the optimal pH adjustment of the reaction we used 2-amino-2 methyl -1-propanol (AMP). ALP was within permitted values from 100 to 290 U /l, whereas Gamma-glutamyl Transferase was at normal reference values of 5 to 51 U /l. Automated method was equal to the standard method, with a correlation coefficient of r = 0997. Accuracy within the series of measurements had a variation coefficient of only 1.25%. From the results taken from the laboratories, we had an increased precision, accuracy, sensitivity and reliability as a result of the technological process of electronic engineering of new modern techniques with monoclonal antibodies and immobilized enzymes.},
 year = {2015}
}

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