The Efficiency of Intrauterine Irrigation of the Cavitated Drug Solutes in Patients with Endometrial Receptivity Disorders

Oxana Alexandrovna Melkozerova; Evgeniy Yuryevich Gluchov; Nadezda Vasilyevna Bashmakova; Guzel Nuchovna Chistyakova; Alena Valeryevna Lvova; Anna Michaylovna Bogdanova; Aigul Adipovna Giniyatova; Olga Germanovna Barlit

doi:doi:10.11648/j.jgo.20180604.15

The Efficiency of Intrauterine Irrigation of the Cavitated Drug Solutes in Patients with Endometrial Receptivity Disorders

Oxana Alexandrovna Melkozerova, Evgeniy Yuryevich Gluchov, Nadezda Vasilyevna Bashmakova, Guzel Nuchovna Chistyakova, Alena Valeryevna Lvova, Anna Michaylovna Bogdanova, Aigul Adipovna Giniyatova, Olga Germanovna Barlit

Published in Journal of Gynecology and Obstetrics (Volume 6, Issue 4)

Received: 6 June 2018 Accepted: 12 July 2018 Published: 17 September 2018

Views: Downloads:

Download PDF

Share This Article

Twitter
Linked In
Facebook

Abstract

Evaluate the efficiency of intrauterine irrigation of cavitated low-frequency sonication drug solution in restoring of endometrial receptivity in patients with uterine infertility. 92 women with uterine infertility due to endometrial hypoplasia were examined in the dynamics of therapy including irrigation of the uterine cavity with cavitated low-frequency ultrasound with drug solutions. The control group consisted of 28 healthy fertile women. An ultrasound examination of the pelvic organs with color dopplerogragy, histological and immunohistochemical study of the endometrium was performed on the "implantation window" LH + 7, in dynamics before the start of therapy and in the next cycle after the end of treatment. After cavitation irrigation of the uterine cavity, 62 (67.39%) women with infertility in the endometrial specimens had adequate vascularization of the stroma (before treatment, 36.95%, p=0.017, in control 78.57%), in 68 (73, 91%) women - a decrease in the density of the stromal matrix, in 64 (69.56%) - according to light microscopy data, mature pinopodia were determined (initially 28.26%, p=0.0015). On the background of therapy, the ratio of ERα / PR expression in the stroma of the implantation endometrium was normalized, which was characterized by the prevalence of the PR pool (1.01±0.34 before treatment, 0.36 ± 0.03 after therapy, p = 0.040, in control 0.34±0.06, p> 0.05). The parameters of stromal expression of natural uterine killers CD56 + bright against the background of treatment in patients with infertility come in accordance with the indices of healthy fertile women (from 35.21 ± 2.14 cells in l/s to 45.75±3.18 cells l/s; in the control 47.8±2.13 cells in l/s; p> 0.05). A significant decrease in stromal expression of CD3+ is noted: 78.17±6.89 cells in l/s versus 23.83±3.63 cells in l/s; p=0.004. The expression level of CD34 in the endometrial stroma after the treatment was significantly increased (20.88±0.77 cells in l/s versus 33.83±3.63 cells in l/s, p=0.035). The use of G-CSF cavitaion solutions for irrigation of the uterine cavity appears to be effective in restoring tissue and molecular endometrial receptivity in patients with uterine infertility due to endometrial hypoplasia.

Published in	Journal of Gynecology and Obstetrics (Volume 6, Issue 4)
DOI	10.11648/j.jgo.20180604.15
Page(s)	98-107
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2018. Published by Science Publishing Group

Keywords

Uterine Infertility, Endometrial Hypoplasia, Cavitation Irrigation of Uterine Cavity, Granulocyte Colony-Stimulating Factor

References

[1]	The European health report 2015. Targets and beyond – reaching new frontiers in evidence. Copenhagen: WHO Regional Office for Europe. 2015. Accessed July 08. 2018. http://www.euro.who.int/en/data-andevidence
[2]	Health-2020: a European policy framework and strategy for the 21st century. Copenhagen: WHO Regional Office for Europe; 2013. Accessed July 08. 2018. http://www.euro.who.int/en/publications/abstracts/health-2020-a-european-policy-framework-and-strategy-for-the-21stcentury
[3]	Ermolenko. K. S. Radzinskij V. E. Rapoport S. I. The current state of the problem of the realization of the fertile function of women of late reproductive age. [Klinicheskaya medicina]. 2016; 94(1):10-15. (In Russ.). Accessed July 08. 2018. http://www.medlit.ru/journalsview/clinicalmedicine/view/journal/2016/issue-1/69-sovremennoe-sostoyanie-problemy-realizacii-fertil-noy-funkcii-zhenschin-pozdnego-reproduktivnogo-vozrasta
[4]	Register ART RAHR. Report for 2016. SP-b; 2017. (in Russ.). Accessed July 08. 2018. http://www.rahr.ru/d_registr_otchet/registr_BRT_RARCH16.pdf
[5]	Valdes CT. Schutt A. Simon C. Implantation failure of endometrial origin: it is not pathology. but our failure to synchronize the developing embryo with a receptive endometrium. Fertil Steril. 2017 Jul; 108(1):15-18. https://doi.org/10.1016/j.fertnstert.2017.05.033
[6]	Boyarskij K. YU. Gajdukov S. N. Pal'chenko N. A. Modern view on the problem of receptivity and thin endometrium in ART programs. Literature review. [Problemy reprodukcii]. 2013; 4:3-7. (in Russ.). Accessed July 08. 2018. https://www.mediasphera.ru/issues/problemy-reproduktsii/2013/4/031025-72172013411
[7]	Uterine Endometrial Function. Editor of H. Kanzaki. Osaka: Springer; 2017.
[8]	Molecular mechanisms of the reproductive system diseases. Editor of M. A. Pal'cev. SPb.: Eko-vektor; 2017. (in Russ.).
[9]	Pregravid preparation: the clinical protocol. Editor of V.E. Radzinskij. М.: Status Praesens; 2016: 23-25. (in Russ.). Accessed July 08. 2018. https://praesens.ru/pregravidarnaya-podgotovka-klinicheskiy-protokol-mezhdistsiplinarnoy-assotsiatsii-spetsialistov-repr
[10]	Krylova YU. M. Kvetnoj I. M. Ajlamazyan Eh.K. Receptivity of the endometrium: molecular mechanisms of regulation of implantation. [Zhurnal akusherstva i zhenskih boleznej]. 2013; 2: 63-74. https://doi.org/10.17816/JOWD663135-142
[11]	Li Z. L. Ueki K. Kumagai K. Regulation of bcl-2 transcription by estrogen receptor-alpha and C-Jun in human endometrium. Med. Mol. Morphol. 2014; 47(1): 43-53. https://doi.10.1007/s00795-013-0043-y
[12]	Ogata T. Ito K. Shindo T. Hatanaka K. Eguchi K. Kurosawa R. Kagaya Y. Monma Y. Ichijo S. Taki H. Kanai H. Shimokawa H. Low-intensity pulsed ultrasound enhances angiogenesis and ameliorates contractile dysfunction of pressure-overloaded heart in mice. PLoS One. 2017; 12(9):e0185555. https://doi.org/10.1371/journal.pone.0185555
[13]	Wu S. Xu X. Sun J. Zhang Y. Shi J. Xu T. Low-Intensity Pulsed Ultrasound Accelerates Traumatic Vertebral Fracture Healing by Coupling Proliferation of Type H Microvessels. J Ultrasound Med. 2018 Jan 24. https://doi.org/10.1002/jum.14525
[14]	Bogdanova AM. Glukhov E.Yu. Dicke G.B. Application of low-frequency ultrasound in the complex treatment of the hypoplastic variant of chronic endometritis. Obstetrics and gynecology. 2017; 2: 90-6. https://dx.doi.org/10.18565/aig.2017.2.90-6
[15]	Altland O.D. Dalecki D. Suchkova V.N. Low-intensity ultrasound increases endothelial cell nitric oxide syntase activity and nitric oxide synthesis. J. Thromb. Haemost. 2004; 2:637–643. https://doi.org/10.1111/j.1538-7836.2004.00655.x
[16]	Hanawa K. Ito K. Aizawa K. Low-intensity pulsed ultrasound induces angiogenesis and ameliorates left ventricular dysfunction in a porcine model of chronic myocardial ischemia. PLoS One. 2014; (9):8:104-863. https://doi.org/10.1371/journal.pone.0104863. eCollection 2014.
[17]	Shindo T. Ito K. Ogata T. Low-Intensity Pulsed Ultrasound Enhances Angiogenesis and Ameliorates Left Ventricular Dysfunction in a Mouse Model of Acute Myocardial Infarction. Arterioscler. Thromb. Vasc. Biol. 2016; (36):6:1220-1229. https://doi.org/10.1161/ATVBAHA.115.306477
[18]	Huang J. I. A. Shi Y. O. Li R. L. Angiogenesis effect of therapeutic ultrasound on HUVECs through activation of the PI3K-Akt-eNOS signal pathway. Am. J. Transl. Res. 2015; (7):6:1106-1115. Accessed July 08. 2018. http://www.ajtr.org/files/ajtr0004723.pdf
[19]	Cleicher N. Vidali A. Barad D.H. Successful treatment of unreceptive thin endometrium. Fertill Sterill. 2011; (95):6:2123-2137. https://doi.org/10.1093/humrep/des370
[20]	Jerman L. F. Hey-Cunningham A. The role of the lymphatic system in endometriosis: a comprehensive review of the literature. J. Biol. Reprod. 2015; (92):3:64-66. https://doi.org/10.1095/biolreprod.114.124313
[21]	Koukourakis M. I. Giatromanolaki A. Sivridis E. LYVE-1 immunohistochemical assessment of lymphangiogenesis in endometrial and lung cancer. J. Clin. Pathol. 2015; (58):2:202-206. https://doi.org/10.1136/jcp.2004.019174
[22]	Uterine Endometrial Function / Editor of H. Kanzaki. Osaka: Springer. 2017; 155 р. Accessed July 08. 2018. https://www.springer.com/gp/book/9784431559702
[23]	Simón C. Giudice LC. The Endometrial Factor: A Reproductive Precision Medicine Approach. CRC Press. Apr 27. 2017. Accessed July 08. 2018. https://www.crcpress.com/The-Essential-Endometrium-Handbook-Current-Applications-in-Clinical-Medicine/Simon-Giudice/p/book/9781498740395

Cite This Article

Plain Text BibTeX RIS

APA Style

Oxana Alexandrovna Melkozerova, Evgeniy Yuryevich Gluchov, Nadezda Vasilyevna Bashmakova, Guzel Nuchovna Chistyakova, Alena Valeryevna Lvova, et al. (2018). The Efficiency of Intrauterine Irrigation of the Cavitated Drug Solutes in Patients with Endometrial Receptivity Disorders. Journal of Gynecology and Obstetrics, 6(4), 98-107. https://doi.org/10.11648/j.jgo.20180604.15

Copy | Download

ACS Style

Oxana Alexandrovna Melkozerova; Evgeniy Yuryevich Gluchov; Nadezda Vasilyevna Bashmakova; Guzel Nuchovna Chistyakova; Alena Valeryevna Lvova, et al. The Efficiency of Intrauterine Irrigation of the Cavitated Drug Solutes in Patients with Endometrial Receptivity Disorders. J. Gynecol. Obstet. 2018, 6(4), 98-107. doi: 10.11648/j.jgo.20180604.15

Copy | Download

AMA Style

Oxana Alexandrovna Melkozerova, Evgeniy Yuryevich Gluchov, Nadezda Vasilyevna Bashmakova, Guzel Nuchovna Chistyakova, Alena Valeryevna Lvova, et al. The Efficiency of Intrauterine Irrigation of the Cavitated Drug Solutes in Patients with Endometrial Receptivity Disorders. J Gynecol Obstet. 2018;6(4):98-107. doi: 10.11648/j.jgo.20180604.15

Copy | Download

@article{10.11648/j.jgo.20180604.15,
  author = {Oxana Alexandrovna Melkozerova and Evgeniy Yuryevich Gluchov and Nadezda Vasilyevna Bashmakova and Guzel Nuchovna Chistyakova and Alena Valeryevna Lvova and Anna Michaylovna Bogdanova and Aigul Adipovna Giniyatova and Olga Germanovna Barlit},
  title = {The Efficiency of Intrauterine Irrigation of the Cavitated Drug Solutes in Patients with Endometrial Receptivity Disorders},
  journal = {Journal of Gynecology and Obstetrics},
  volume = {6},
  number = {4},
  pages = {98-107},
  doi = {10.11648/j.jgo.20180604.15},
  url = {https://doi.org/10.11648/j.jgo.20180604.15},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jgo.20180604.15},
  abstract = {Evaluate the efficiency of intrauterine irrigation of cavitated low-frequency sonication drug solution in restoring of endometrial receptivity in patients with uterine infertility. 92 women with uterine infertility due to endometrial hypoplasia were examined in the dynamics of therapy including irrigation of the uterine cavity with cavitated low-frequency ultrasound with drug solutions. The control group consisted of 28 healthy fertile women. An ultrasound examination of the pelvic organs with color dopplerogragy, histological and immunohistochemical study of the endometrium was performed on the "implantation window" LH + 7, in dynamics before the start of therapy and in the next cycle after the end of treatment. After cavitation irrigation of the uterine cavity, 62 (67.39%) women with infertility in the endometrial specimens had adequate vascularization of the stroma (before treatment, 36.95%, p=0.017, in control 78.57%), in 68 (73, 91%) women - a decrease in the density of the stromal matrix, in 64 (69.56%) - according to light microscopy data, mature pinopodia were determined (initially 28.26%, p=0.0015). On the background of therapy, the ratio of ERα / PR expression in the stroma of the implantation endometrium was normalized, which was characterized by the prevalence of the PR pool (1.01±0.34 before treatment, 0.36 ± 0.03 after therapy, p = 0.040, in control 0.34±0.06, p> 0.05). The parameters of stromal expression of natural uterine killers CD56 + bright against the background of treatment in patients with infertility come in accordance with the indices of healthy fertile women (from 35.21 ± 2.14 cells in l/s to 45.75±3.18 cells l/s; in the control 47.8±2.13 cells in l/s; p> 0.05). A significant decrease in stromal expression of CD3+ is noted: 78.17±6.89 cells in l/s versus 23.83±3.63 cells in l/s; p=0.004. The expression level of CD34 in the endometrial stroma after the treatment was significantly increased (20.88±0.77 cells in l/s versus 33.83±3.63 cells in l/s, p=0.035). The use of G-CSF cavitaion solutions for irrigation of the uterine cavity appears to be effective in restoring tissue and molecular endometrial receptivity in patients with uterine infertility due to endometrial hypoplasia.},
 year = {2018}
}

Copy | Download

TY  - JOUR
T1  - The Efficiency of Intrauterine Irrigation of the Cavitated Drug Solutes in Patients with Endometrial Receptivity Disorders
AU  - Oxana Alexandrovna Melkozerova
AU  - Evgeniy Yuryevich Gluchov
AU  - Nadezda Vasilyevna Bashmakova
AU  - Guzel Nuchovna Chistyakova
AU  - Alena Valeryevna Lvova
AU  - Anna Michaylovna Bogdanova
AU  - Aigul Adipovna Giniyatova
AU  - Olga Germanovna Barlit
Y1  - 2018/09/17
PY  - 2018
N1  - https://doi.org/10.11648/j.jgo.20180604.15
DO  - 10.11648/j.jgo.20180604.15
T2  - Journal of Gynecology and Obstetrics
JF  - Journal of Gynecology and Obstetrics
JO  - Journal of Gynecology and Obstetrics
SP  - 98
EP  - 107
PB  - Science Publishing Group
SN  - 2376-7820
UR  - https://doi.org/10.11648/j.jgo.20180604.15
AB  - Evaluate the efficiency of intrauterine irrigation of cavitated low-frequency sonication drug solution in restoring of endometrial receptivity in patients with uterine infertility. 92 women with uterine infertility due to endometrial hypoplasia were examined in the dynamics of therapy including irrigation of the uterine cavity with cavitated low-frequency ultrasound with drug solutions. The control group consisted of 28 healthy fertile women. An ultrasound examination of the pelvic organs with color dopplerogragy, histological and immunohistochemical study of the endometrium was performed on the "implantation window" LH + 7, in dynamics before the start of therapy and in the next cycle after the end of treatment. After cavitation irrigation of the uterine cavity, 62 (67.39%) women with infertility in the endometrial specimens had adequate vascularization of the stroma (before treatment, 36.95%, p=0.017, in control 78.57%), in 68 (73, 91%) women - a decrease in the density of the stromal matrix, in 64 (69.56%) - according to light microscopy data, mature pinopodia were determined (initially 28.26%, p=0.0015). On the background of therapy, the ratio of ERα / PR expression in the stroma of the implantation endometrium was normalized, which was characterized by the prevalence of the PR pool (1.01±0.34 before treatment, 0.36 ± 0.03 after therapy, p = 0.040, in control 0.34±0.06, p> 0.05). The parameters of stromal expression of natural uterine killers CD56 + bright against the background of treatment in patients with infertility come in accordance with the indices of healthy fertile women (from 35.21 ± 2.14 cells in l/s to 45.75±3.18 cells l/s; in the control 47.8±2.13 cells in l/s; p> 0.05). A significant decrease in stromal expression of CD3+ is noted: 78.17±6.89 cells in l/s versus 23.83±3.63 cells in l/s; p=0.004. The expression level of CD34 in the endometrial stroma after the treatment was significantly increased (20.88±0.77 cells in l/s versus 33.83±3.63 cells in l/s, p=0.035). The use of G-CSF cavitaion solutions for irrigation of the uterine cavity appears to be effective in restoring tissue and molecular endometrial receptivity in patients with uterine infertility due to endometrial hypoplasia.
VL  - 6
IS  - 4
ER  -

Copy | Download

Author Information

Oxana Alexandrovna Melkozerova

Ural Research Institute of Maternity and Child Care Russian Ministry of Health, Ekaterinburg, Russia
Evgeniy Yuryevich Gluchov

Ural State Medical University Russian Ministry of Health, Ekaterinburg, Russia
Nadezda Vasilyevna Bashmakova

Ural Research Institute of Maternity and Child Care Russian Ministry of Health, Ekaterinburg, Russia
Guzel Nuchovna Chistyakova

Ural Research Institute of Maternity and Child Care Russian Ministry of Health, Ekaterinburg, Russia
Alena Valeryevna Lvova

Ural Research Institute of Maternity and Child Care Russian Ministry of Health, Ekaterinburg, Russia
Anna Michaylovna Bogdanova

Ural State Medical University Russian Ministry of Health, Ekaterinburg, Russia
Aigul Adipovna Giniyatova

Ural Research Institute of Maternity and Child Care Russian Ministry of Health, Ekaterinburg, Russia
Olga Germanovna Barlit

Ural State Medical University Russian Ministry of Health, Ekaterinburg, Russia

Download PDF

Sections

Plain Text BibTeX RIS

APA Style

Oxana Alexandrovna Melkozerova, Evgeniy Yuryevich Gluchov, Nadezda Vasilyevna Bashmakova, Guzel Nuchovna Chistyakova, Alena Valeryevna Lvova, et al. (2018). The Efficiency of Intrauterine Irrigation of the Cavitated Drug Solutes in Patients with Endometrial Receptivity Disorders. Journal of Gynecology and Obstetrics, 6(4), 98-107. https://doi.org/10.11648/j.jgo.20180604.15

Copy | Download

ACS Style

Oxana Alexandrovna Melkozerova; Evgeniy Yuryevich Gluchov; Nadezda Vasilyevna Bashmakova; Guzel Nuchovna Chistyakova; Alena Valeryevna Lvova, et al. The Efficiency of Intrauterine Irrigation of the Cavitated Drug Solutes in Patients with Endometrial Receptivity Disorders. J. Gynecol. Obstet. 2018, 6(4), 98-107. doi: 10.11648/j.jgo.20180604.15

Copy | Download

AMA Style

Oxana Alexandrovna Melkozerova, Evgeniy Yuryevich Gluchov, Nadezda Vasilyevna Bashmakova, Guzel Nuchovna Chistyakova, Alena Valeryevna Lvova, et al. The Efficiency of Intrauterine Irrigation of the Cavitated Drug Solutes in Patients with Endometrial Receptivity Disorders. J Gynecol Obstet. 2018;6(4):98-107. doi: 10.11648/j.jgo.20180604.15

Copy | Download

@article{10.11648/j.jgo.20180604.15,
  author = {Oxana Alexandrovna Melkozerova and Evgeniy Yuryevich Gluchov and Nadezda Vasilyevna Bashmakova and Guzel Nuchovna Chistyakova and Alena Valeryevna Lvova and Anna Michaylovna Bogdanova and Aigul Adipovna Giniyatova and Olga Germanovna Barlit},
  title = {The Efficiency of Intrauterine Irrigation of the Cavitated Drug Solutes in Patients with Endometrial Receptivity Disorders},
  journal = {Journal of Gynecology and Obstetrics},
  volume = {6},
  number = {4},
  pages = {98-107},
  doi = {10.11648/j.jgo.20180604.15},
  url = {https://doi.org/10.11648/j.jgo.20180604.15},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jgo.20180604.15},
  abstract = {Evaluate the efficiency of intrauterine irrigation of cavitated low-frequency sonication drug solution in restoring of endometrial receptivity in patients with uterine infertility. 92 women with uterine infertility due to endometrial hypoplasia were examined in the dynamics of therapy including irrigation of the uterine cavity with cavitated low-frequency ultrasound with drug solutions. The control group consisted of 28 healthy fertile women. An ultrasound examination of the pelvic organs with color dopplerogragy, histological and immunohistochemical study of the endometrium was performed on the "implantation window" LH + 7, in dynamics before the start of therapy and in the next cycle after the end of treatment. After cavitation irrigation of the uterine cavity, 62 (67.39%) women with infertility in the endometrial specimens had adequate vascularization of the stroma (before treatment, 36.95%, p=0.017, in control 78.57%), in 68 (73, 91%) women - a decrease in the density of the stromal matrix, in 64 (69.56%) - according to light microscopy data, mature pinopodia were determined (initially 28.26%, p=0.0015). On the background of therapy, the ratio of ERα / PR expression in the stroma of the implantation endometrium was normalized, which was characterized by the prevalence of the PR pool (1.01±0.34 before treatment, 0.36 ± 0.03 after therapy, p = 0.040, in control 0.34±0.06, p> 0.05). The parameters of stromal expression of natural uterine killers CD56 + bright against the background of treatment in patients with infertility come in accordance with the indices of healthy fertile women (from 35.21 ± 2.14 cells in l/s to 45.75±3.18 cells l/s; in the control 47.8±2.13 cells in l/s; p> 0.05). A significant decrease in stromal expression of CD3+ is noted: 78.17±6.89 cells in l/s versus 23.83±3.63 cells in l/s; p=0.004. The expression level of CD34 in the endometrial stroma after the treatment was significantly increased (20.88±0.77 cells in l/s versus 33.83±3.63 cells in l/s, p=0.035). The use of G-CSF cavitaion solutions for irrigation of the uterine cavity appears to be effective in restoring tissue and molecular endometrial receptivity in patients with uterine infertility due to endometrial hypoplasia.},
 year = {2018}
}

Copy | Download

TY  - JOUR
T1  - The Efficiency of Intrauterine Irrigation of the Cavitated Drug Solutes in Patients with Endometrial Receptivity Disorders
AU  - Oxana Alexandrovna Melkozerova
AU  - Evgeniy Yuryevich Gluchov
AU  - Nadezda Vasilyevna Bashmakova
AU  - Guzel Nuchovna Chistyakova
AU  - Alena Valeryevna Lvova
AU  - Anna Michaylovna Bogdanova
AU  - Aigul Adipovna Giniyatova
AU  - Olga Germanovna Barlit
Y1  - 2018/09/17
PY  - 2018
N1  - https://doi.org/10.11648/j.jgo.20180604.15
DO  - 10.11648/j.jgo.20180604.15
T2  - Journal of Gynecology and Obstetrics
JF  - Journal of Gynecology and Obstetrics
JO  - Journal of Gynecology and Obstetrics
SP  - 98
EP  - 107
PB  - Science Publishing Group
SN  - 2376-7820
UR  - https://doi.org/10.11648/j.jgo.20180604.15
AB  - Evaluate the efficiency of intrauterine irrigation of cavitated low-frequency sonication drug solution in restoring of endometrial receptivity in patients with uterine infertility. 92 women with uterine infertility due to endometrial hypoplasia were examined in the dynamics of therapy including irrigation of the uterine cavity with cavitated low-frequency ultrasound with drug solutions. The control group consisted of 28 healthy fertile women. An ultrasound examination of the pelvic organs with color dopplerogragy, histological and immunohistochemical study of the endometrium was performed on the "implantation window" LH + 7, in dynamics before the start of therapy and in the next cycle after the end of treatment. After cavitation irrigation of the uterine cavity, 62 (67.39%) women with infertility in the endometrial specimens had adequate vascularization of the stroma (before treatment, 36.95%, p=0.017, in control 78.57%), in 68 (73, 91%) women - a decrease in the density of the stromal matrix, in 64 (69.56%) - according to light microscopy data, mature pinopodia were determined (initially 28.26%, p=0.0015). On the background of therapy, the ratio of ERα / PR expression in the stroma of the implantation endometrium was normalized, which was characterized by the prevalence of the PR pool (1.01±0.34 before treatment, 0.36 ± 0.03 after therapy, p = 0.040, in control 0.34±0.06, p> 0.05). The parameters of stromal expression of natural uterine killers CD56 + bright against the background of treatment in patients with infertility come in accordance with the indices of healthy fertile women (from 35.21 ± 2.14 cells in l/s to 45.75±3.18 cells l/s; in the control 47.8±2.13 cells in l/s; p> 0.05). A significant decrease in stromal expression of CD3+ is noted: 78.17±6.89 cells in l/s versus 23.83±3.63 cells in l/s; p=0.004. The expression level of CD34 in the endometrial stroma after the treatment was significantly increased (20.88±0.77 cells in l/s versus 33.83±3.63 cells in l/s, p=0.035). The use of G-CSF cavitaion solutions for irrigation of the uterine cavity appears to be effective in restoring tissue and molecular endometrial receptivity in patients with uterine infertility due to endometrial hypoplasia.
VL  - 6
IS  - 4
ER  -

Copy | Download