Background and Aim: Oral squamous cell carcinoma (OSCC) is a growing health problem worldwide, for which standard treatment strategies have failed to increase its recovery rate significantly. In recent decades, many studies have investigated herbal or natural compounds for the prevention and treatment of OSCC. The present study was performed to investigate the effects of the extract of Portulaca oleracea seed, Berberis vulgaris, and Vaccinium myrtillus fruit on the OSCC. Experimental procedure: Firstly, the herbal samples were purchased from the medicinal plant market and each one of the samples was extracted. Afterward, SCC-15 cells were purchased from the Iranian Biological Resource Center. MTT test was used to evaluate cell proliferation and to measure the transcription rate of ki67, miR-4492, and miR-877-5p genes using Real-time PCR. Finally, the western blot test was used to measure the translation rate of b-cell lymphoma 2 and matrix metalloproteinase-2 genes. Quantitative variables of the present study were described as mean and standard deviation and the three-way ANOVA was used via SPSS ver. 25. an independent t-test was used. P-value< 0.05 was considered a statistically significant level. Results and Conclusion: Briefly, the findings of the present study showed that all three extracts had significant effects on growth suppression, apoptosis induction, and OSCC metastasis. Also, the triple (PVB) combination and Portulaca-Berberis (PB) combination were shown to be the most effective. This result indicates the anti-cancer synergistic effect of these extracts especially P and B, on suppressing OSCC, which should be confirmed in future animal and human studies.
| Published in | Rehabilitation Science (Volume 6, Issue 3) |
| DOI | 10.11648/j.rs.20210603.11 |
| Page(s) | 41-48 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2021. Published by Science Publishing Group |
Herbal Compound, Apoptosis, Cell Proliferation, Neoplasm Metastasis, Micro RNA
| [1] | Shibahara T. Oral cancer-diagnosis and therapy. Clin Calcium. 2017; 27 (10): 1427-33. |
| [2] | Sudbø J, Reith A. Retracted: The evolution of predictive oncology and molecular‐based therapy for oral cancer prevention. Int J Cancer. 2005; 115 (3): 339-45. |
| [3] | Gharat SA, Momin M, Bhavsar C. Oral squamous cell carcinoma: current treatment strategies and nanotechnology-based approaches for prevention and therapy. CRIT REV THER DRUG. 2016; 33 (4). |
| [4] | Eastham LL, Howard CM, Balachandran P, Pasco DS, Claudio PP. Eating green: shining light on the use of dietary phytochemicals as a modern approach in the prevention and treatment of head and neck cancers. Curr Top Med Chem. 2018; 18 (3): 182-91. |
| [5] | Shan Z, Yang G, Xiang W, Pei-jun W, Bin Z. Effects of resveratrol on oral squamous cell carcinoma (OSCC) cells in vitro. J Cancer Res Clin Oncol. 2014; 140 (3): 371-4. |
| [6] | Dias D, Atukorala C, Amaratunga NdS, Perera B, Karunathilake L. The effects of Emblica officinalis and Tinospora cordifolia herbal treatment on the prognosis of squamous cell carcinoma of the buccal mucosa and tongue. J Oral Maxillofac Surg Med Pathol. 2018; 30 (1): 21-9. |
| [7] | Wang H, Zhu L, Feng X, Zhang H, Luo Q, Chen F. Oridonin induces G2/M cell cycle arrest and apoptosis in human oral squamous cell carcinoma. Eur J Pharmacol. 2017; 815: 282-9. |
| [8] | WARNER B, KNOBLOCH T, CASTO B, HOLPUCH A, ACCURSO B, SUMMERSGILL K, et al. Topical Black Raspberries Reduce Proliferation and Survivin/Birc5 in Established OSCC. Oral Surg Oral Med Oral Pathol Oral Radiol. 2015; 120 (3): e140. |
| [9] | Srivastava S, Mohammad S, Pant AB, Mishra PR, Pandey G, Gupta S, et al. Co-delivery of 5-fluorouracil and curcumin nanohybrid formulations for improved chemotherapy against oral squamous cell carcinoma. J Maxillofac Oral Surg. 2018; 17 (4): 597-610. |
| [10] | Uddin M, Juraimi AS, Hossain MS, Un A, Ali M, Rahman M. Purslane weed (Portulaca oleracea): a prospective plant source of nutrition, omega-3 fatty acid, and antioxidant attributes. Sci World J. 2014; 2014. |
| [11] | Guo G, Yue L, Fan S, Jing S, Yan L-J. Antioxidant and antiproliferative activities of Purslane seed oil. J Hypertens. 2016; 5 (2). |
| [12] | Farshori NN, Al-Sheddi ES, Al-Oqail MM, Musarrat J, Al-Khedhairy AA, Siddiqui MA. Cytotoxicity Assessments of Portulaca oleraceaand Petroselinum sativum Seed Extracts on Human Hepatocellular Carcinoma Cells (HepG2). Asian Pac J Cancer Prev. 2014; 15 (16): 6633-8. |
| [13] | Guo N, Cheng F, Wu J, Liu B, Zheng S, Liang J, et al. Anthocyanin biosynthetic genes in Brassica rapa. BMC Genomics. 2014; 15 (1): 426. |
| [14] | Martz F, Jaakola L, Julkunen-Tiitto R, Stark S. Phenolic composition and antioxidant capacity of bilberry (Vaccinium myrtillus) leaves in Northern Europe following foliar development and along environmental gradients. J Chem Ecol. 2010; 36 (9): 1017-28. |
| [15] | Katsube N, Iwashita K, Tsushida T, Yamaki K, Kobori M. Induction of apoptosis in cancer cells by bilberry (Vaccinium myrtillus) and the anthocyanins. J Agric Food Chem. 2003; 51 (1): 68-75. |
| [16] | Rad SZK, Rameshrad M, Hosseinzadeh H. Toxicology effects of Berberis vulgaris (barberry) and its active constituent, berberine: a review. IRAN J BASIC MED SCI. 2017; 20 (5): 516. |
| [17] | Zhao Y, Jing Z, Li Y, Mao W. Berberine in combination with cisplatin suppresses breast cancer cell growth through induction of DNA breaks and caspase-3-dependent apoptosis. Oncol Rep. 2016; 36 (1): 567-72. |
| [18] | Abd El-Wahab AE, Ghareeb DA, Sarhan EE, Abu-Serie MM, El Demellawy MA. In vitro biological assessment of Berberis vulgaris and its active constituent, berberine: antioxidants, anti-acetylcholinesterase, anti-diabetic and anticancer effects. BMC Complement Altern Med. 2013; 13 (1): 218. |
| [19] | Wang C, Ma H-X, Jin M-S, Zou Y-B, Teng Y-L, Tian Z, et al. Association of matrix metalloproteinase (MMP)-2 and-9 expression with extra-gastrointestinal stromal tumor metastasis. Asian Pac J Cancer Prev. 2014; 15 (10): 4187-92. |
| [20] | Thomas G, Lewis M, Speight P. Matrix metalloproteinases and oral cancer. Oral Oncol. 1999; 35 (3): 227-33. |
| [21] | Patel BP, Shah PM, Rawal UM, Desai AA, Shah SV, Rawal RM, et al. Activation of MMP‐2 and MMP‐9 in patients with oral squamous cell carcinoma. J Surg Oncol. 2005; 90 (2): 81-8. |
| [22] | Czabotar PE, Lessene G, Strasser A, Adams JM. Control of apoptosis by the BCL-2 protein family: implications for physiology and therapy. Nat Rev Mol. 2014; 15 (1): 49-63. |
| [23] | Suresh K, Manoharan S, Vijayaanand MA, Sugunadevi G. Chemopreventive and antioxidant efficacy of (6)-paradol in 7, 12-dimethylbenz (a) anthracene induced hamster buccal pouch carcinogenesis. Pharmacol Rep. 2010; 62 (6): 1178-85. |
| [24] | Volinia S, Calin GA, Liu C-G, Ambs S, Cimmino A, Petrocca F, et al. A microRNA expression signature of human solid tumors defines cancer gene targets. Proc Natl Acad Sci. 2006; 103 (7): 2257-61. |
| [25] | Bartel DP. MicroRNAs: genomics, biogenesis, mechanism, and function. Cell. 2004; 116 (2): 281-97. |
| [26] | Calin GA, Croce CM. MicroRNA signatures in human cancers. Nat Rev Cancer. 2006; 6 (11): 857-66. |
| [27] | Calin GA, Croce CM. MicroRNA-cancer connection: the beginning of a new tale. Cancer Res. 2006; 66 (15): 7390-4. |
| [28] | Schneider A, Victoria B, Lopez YN, Suchorska W, Barczak W, Sobecka A, et al. Tissue and serum microRNA profile of oral squamous cell carcinoma patients. Sci Rep. 2018; 8 (1): 1-8. |
| [29] | Ren K, Zhang W, Wu G, Ren J, Lu H, Li Z, et al. Synergistic anti-cancer effects of galangin and berberine through apoptosis induction and proliferation inhibition in oesophageal carcinoma cells. Biomed Pharmacother. 2016; 84: 1748-59. |
| [30] | Tumbas V, Čanadanović-Brunet J, Gille L, Đilas S, Ćetković G. Superoxide anion radical scavenging activity of bilberry (Vaccinium myrtillus L.). J BERRY RES. 2010; 1 (1): 13-23. |
| [31] | Hassan A. Chemical and remedial effects of purslane (portulaca oleracea) plant. Life Sci J. 2014; 11: 31-42. |
| [32] | Ali HA, Hussein MA, Mohammed WE. Comparative effects of purslane seed oil (PSO) and 5-Flourourasil on ehrlich ascites carcinoma (EAC) in female albino mice. Int J Pharma Sci. 2014; 4: 424-30. |
| [33] | Hoshyar R, Mahboob Z, Zarban A. The antioxidant and chemical properties of Berberis vulgaris and its cytotoxic effect on human breast carcinoma cells. Cytotechnology. 2016; 68 (4): 1207-13. |
| [34] | Nguyen V, Tang J, Oroudjev E, Lee CJ, Marasigan C, Wilson L, et al. Cytotoxic effects of bilberry extract on MCF7-GFP-tubulin breast cancer cells. J Med Food. 2010; 13 (2): 278-85. |
| [35] | Mikulic‐Petkovsek M, Schmitzer V, Slatnar A, Stampar F, Veberic R. A comparison of fruit quality parameters of wild bilberry (Vaccinium myrtillus L.) growing at different locations. J Sci Food Agric. 2015; 95 (4): 776-85. |
APA Style
Sara Pourshahidi, Mohaddeseh Davari, Naghmeh Bahrami, Roja Rahimi. (2021). Investigating and Comparing the Effect of Portulaca oleracea, Vaccinium myrtillus, and Berberis vulgaris on Oral Squamous Cell Carcinoma in an Vitro Study. Rehabilitation Science, 6(3), 41-48. https://doi.org/10.11648/j.rs.20210603.11
ACS Style
Sara Pourshahidi; Mohaddeseh Davari; Naghmeh Bahrami; Roja Rahimi. Investigating and Comparing the Effect of Portulaca oleracea, Vaccinium myrtillus, and Berberis vulgaris on Oral Squamous Cell Carcinoma in an Vitro Study. Rehabil. Sci. 2021, 6(3), 41-48. doi: 10.11648/j.rs.20210603.11
AMA Style
Sara Pourshahidi, Mohaddeseh Davari, Naghmeh Bahrami, Roja Rahimi. Investigating and Comparing the Effect of Portulaca oleracea, Vaccinium myrtillus, and Berberis vulgaris on Oral Squamous Cell Carcinoma in an Vitro Study. Rehabil Sci. 2021;6(3):41-48. doi: 10.11648/j.rs.20210603.11
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Download@article{10.11648/j.rs.20210603.11,
author = {Sara Pourshahidi and Mohaddeseh Davari and Naghmeh Bahrami and Roja Rahimi},
title = {Investigating and Comparing the Effect of Portulaca oleracea, Vaccinium myrtillus, and Berberis vulgaris on Oral Squamous Cell Carcinoma in an Vitro Study},
journal = {Rehabilitation Science},
volume = {6},
number = {3},
pages = {41-48},
doi = {10.11648/j.rs.20210603.11},
url = {https://doi.org/10.11648/j.rs.20210603.11},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.rs.20210603.11},
abstract = {Background and Aim: Oral squamous cell carcinoma (OSCC) is a growing health problem worldwide, for which standard treatment strategies have failed to increase its recovery rate significantly. In recent decades, many studies have investigated herbal or natural compounds for the prevention and treatment of OSCC. The present study was performed to investigate the effects of the extract of Portulaca oleracea seed, Berberis vulgaris, and Vaccinium myrtillus fruit on the OSCC. Experimental procedure: Firstly, the herbal samples were purchased from the medicinal plant market and each one of the samples was extracted. Afterward, SCC-15 cells were purchased from the Iranian Biological Resource Center. MTT test was used to evaluate cell proliferation and to measure the transcription rate of ki67, miR-4492, and miR-877-5p genes using Real-time PCR. Finally, the western blot test was used to measure the translation rate of b-cell lymphoma 2 and matrix metalloproteinase-2 genes. Quantitative variables of the present study were described as mean and standard deviation and the three-way ANOVA was used via SPSS ver. 25. an independent t-test was used. P-value< 0.05 was considered a statistically significant level. Results and Conclusion: Briefly, the findings of the present study showed that all three extracts had significant effects on growth suppression, apoptosis induction, and OSCC metastasis. Also, the triple (PVB) combination and Portulaca-Berberis (PB) combination were shown to be the most effective. This result indicates the anti-cancer synergistic effect of these extracts especially P and B, on suppressing OSCC, which should be confirmed in future animal and human studies.},
year = {2021}
}
TY - JOUR T1 - Investigating and Comparing the Effect of Portulaca oleracea, Vaccinium myrtillus, and Berberis vulgaris on Oral Squamous Cell Carcinoma in an Vitro Study AU - Sara Pourshahidi AU - Mohaddeseh Davari AU - Naghmeh Bahrami AU - Roja Rahimi Y1 - 2021/07/15 PY - 2021 N1 - https://doi.org/10.11648/j.rs.20210603.11 DO - 10.11648/j.rs.20210603.11 T2 - Rehabilitation Science JF - Rehabilitation Science JO - Rehabilitation Science SP - 41 EP - 48 PB - Science Publishing Group SN - 2637-594X UR - https://doi.org/10.11648/j.rs.20210603.11 AB - Background and Aim: Oral squamous cell carcinoma (OSCC) is a growing health problem worldwide, for which standard treatment strategies have failed to increase its recovery rate significantly. In recent decades, many studies have investigated herbal or natural compounds for the prevention and treatment of OSCC. The present study was performed to investigate the effects of the extract of Portulaca oleracea seed, Berberis vulgaris, and Vaccinium myrtillus fruit on the OSCC. Experimental procedure: Firstly, the herbal samples were purchased from the medicinal plant market and each one of the samples was extracted. Afterward, SCC-15 cells were purchased from the Iranian Biological Resource Center. MTT test was used to evaluate cell proliferation and to measure the transcription rate of ki67, miR-4492, and miR-877-5p genes using Real-time PCR. Finally, the western blot test was used to measure the translation rate of b-cell lymphoma 2 and matrix metalloproteinase-2 genes. Quantitative variables of the present study were described as mean and standard deviation and the three-way ANOVA was used via SPSS ver. 25. an independent t-test was used. P-value< 0.05 was considered a statistically significant level. Results and Conclusion: Briefly, the findings of the present study showed that all three extracts had significant effects on growth suppression, apoptosis induction, and OSCC metastasis. Also, the triple (PVB) combination and Portulaca-Berberis (PB) combination were shown to be the most effective. This result indicates the anti-cancer synergistic effect of these extracts especially P and B, on suppressing OSCC, which should be confirmed in future animal and human studies. VL - 6 IS - 3 ER -
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