Transdifferentiation of Human Umbilical Cord Stromal Cells to Neurogenic-Like Cells

Pu Jiujun; Wang Zhiming; Ma Xiankun; Zhang Hongdian

doi:doi:10.11648/j.rs.20210604.14

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Transdifferentiation of Human Umbilical Cord Stromal Cells to Neurogenic-Like Cells

Pu Jiujun, Wang Zhiming, Ma Xiankun, Zhang Hongdian

Published in Rehabilitation Science (Volume 6, Issue 4)

Received: 7 December 2021 Accepted: 16 December 2021 Published: 20 December 2021

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Abstract

Objective: To investigate the differentiation of human umbilical cord stromal cells into neurogenic cells. Methods: (1) Human umbilical cord stromal cells (HUMSCs) were pre-induced with recombinant human fibroblast growth factor-2 (hbFGF-2, Peprotech, UK) and N² (1:100, Invitrogen, USA), immunocytochemistry and Western blot to determine the expression of nestin and fibronectin before and after pre-induction. Western blot to identify Nestin and fibronectin expression before and after pre-induction. (2) The differentiation of pre-induced and un-induced HUMSCs into neurogenic cells was performed, and the value-added rates of both were measured by MTT at different time points, and the morphological differences between the differentiated cells and the differentiated cells were verified at the gene and protein levels. Results:(1) 94.4 ± 2.3% and 6.5 ± 1.2% HUMSCs expressed ectodermal marker-fibronectin and stem cell marker-nestin, respectively, and 62.3 ± 5.2% piHUMSCs showed nestin immunopositive staining after adding FGF2 and N² for pre-induction; 51.3 ± 5.9% of cells showed fibronectin immunopositive staining. piHUMSCs showed significantly higher nestin positivity than HUMSCs (P<0.05), whereas HUMSCs expressing fibronectin showed significantly higher positivity than piHUMSCs (P<0.05). Western blot results were consistent with cellular immunohistochemistry results. The proliferation rate of HUMSCs and piHUMSCs by MTT assay suggested that after 48 h of culture, both HUMSCs and piHUMSCs entered the logarithmic cell growth phase, and the cell expansion rate of HUMSCs was significantly higher than that of piHUMSCs after 72 h and 96 h of culture.(2) The results of RT-PCR experiments indicated that differentiated human umbilical cord neural stem cells expressed The expression of the corresponding neural stem cell marker genes was statistically different (p<0.05) than that of undifferentiated umbilical cord stromal cells and terminally differentiated neural cells after induction of fractionation. Conclusion: Umbilical cord-derived stromal cells pre-induced with recombinant human fibroblast growth factor-2 (hbFGF-2, Peprotech, UK) and N² (1:100, Invitrogen, USA) were better able to differentiate into neural stem cells with stable expression of cell morphological changes, proteins and genes.

Published in	Rehabilitation Science (Volume 6, Issue 4)
DOI	10.11648/j.rs.20210604.14
Page(s)	76-82
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2021. Published by Science Publishing Group

Keywords

Cord Blood-derived Neural Stem Cells, Stroke, Nerve Damage, Nestin, Fibronectin

References

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APA Style

Pu Jiujun, Wang Zhiming, Ma Xiankun, Zhang Hongdian. (2021). Transdifferentiation of Human Umbilical Cord Stromal Cells to Neurogenic-Like Cells. Rehabilitation Science, 6(4), 76-82. https://doi.org/10.11648/j.rs.20210604.14

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ACS Style

Pu Jiujun; Wang Zhiming; Ma Xiankun; Zhang Hongdian. Transdifferentiation of Human Umbilical Cord Stromal Cells to Neurogenic-Like Cells. Rehabil. Sci. 2021, 6(4), 76-82. doi: 10.11648/j.rs.20210604.14

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AMA Style

Pu Jiujun, Wang Zhiming, Ma Xiankun, Zhang Hongdian. Transdifferentiation of Human Umbilical Cord Stromal Cells to Neurogenic-Like Cells. Rehabil Sci. 2021;6(4):76-82. doi: 10.11648/j.rs.20210604.14

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@article{10.11648/j.rs.20210604.14,
  author = {Pu Jiujun and Wang Zhiming and Ma Xiankun and Zhang Hongdian},
  title = {Transdifferentiation of Human Umbilical Cord Stromal Cells to Neurogenic-Like Cells},
  journal = {Rehabilitation Science},
  volume = {6},
  number = {4},
  pages = {76-82},
  doi = {10.11648/j.rs.20210604.14},
  url = {https://doi.org/10.11648/j.rs.20210604.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.rs.20210604.14},
  abstract = {Objective: To investigate the differentiation of human umbilical cord stromal cells into neurogenic cells. Methods: (1) Human umbilical cord stromal cells (HUMSCs) were pre-induced with recombinant human fibroblast growth factor-2 (hbFGF-2, Peprotech, UK) and N2 (1:100, Invitrogen, USA), immunocytochemistry and Western blot to determine the expression of nestin and fibronectin before and after pre-induction. Western blot to identify Nestin and fibronectin expression before and after pre-induction. (2) The differentiation of pre-induced and un-induced HUMSCs into neurogenic cells was performed, and the value-added rates of both were measured by MTT at different time points, and the morphological differences between the differentiated cells and the differentiated cells were verified at the gene and protein levels. Results:(1) 94.4 ± 2.3% and 6.5 ± 1.2% HUMSCs expressed ectodermal marker-fibronectin and stem cell marker-nestin, respectively, and 62.3 ± 5.2% piHUMSCs showed nestin immunopositive staining after adding FGF2 and N2 for pre-induction; 51.3 ± 5.9% of cells showed fibronectin immunopositive staining. piHUMSCs showed significantly higher nestin positivity than HUMSCs (P2 (1:100, Invitrogen, USA) were better able to differentiate into neural stem cells with stable expression of cell morphological changes, proteins and genes.},
 year = {2021}
}

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TY  - JOUR
T1  - Transdifferentiation of Human Umbilical Cord Stromal Cells to Neurogenic-Like Cells
AU  - Pu Jiujun
AU  - Wang Zhiming
AU  - Ma Xiankun
AU  - Zhang Hongdian
Y1  - 2021/12/20
PY  - 2021
N1  - https://doi.org/10.11648/j.rs.20210604.14
DO  - 10.11648/j.rs.20210604.14
T2  - Rehabilitation Science
JF  - Rehabilitation Science
JO  - Rehabilitation Science
SP  - 76
EP  - 82
PB  - Science Publishing Group
SN  - 2637-594X
UR  - https://doi.org/10.11648/j.rs.20210604.14
AB  - Objective: To investigate the differentiation of human umbilical cord stromal cells into neurogenic cells. Methods: (1) Human umbilical cord stromal cells (HUMSCs) were pre-induced with recombinant human fibroblast growth factor-2 (hbFGF-2, Peprotech, UK) and N2 (1:100, Invitrogen, USA), immunocytochemistry and Western blot to determine the expression of nestin and fibronectin before and after pre-induction. Western blot to identify Nestin and fibronectin expression before and after pre-induction. (2) The differentiation of pre-induced and un-induced HUMSCs into neurogenic cells was performed, and the value-added rates of both were measured by MTT at different time points, and the morphological differences between the differentiated cells and the differentiated cells were verified at the gene and protein levels. Results:(1) 94.4 ± 2.3% and 6.5 ± 1.2% HUMSCs expressed ectodermal marker-fibronectin and stem cell marker-nestin, respectively, and 62.3 ± 5.2% piHUMSCs showed nestin immunopositive staining after adding FGF2 and N2 for pre-induction; 51.3 ± 5.9% of cells showed fibronectin immunopositive staining. piHUMSCs showed significantly higher nestin positivity than HUMSCs (P2 (1:100, Invitrogen, USA) were better able to differentiate into neural stem cells with stable expression of cell morphological changes, proteins and genes.
VL  - 6
IS  - 4
ER  -

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Author Information

Pu Jiujun

Department of Neurosurgery, Affiliated Sichuan Provincial Rehabilitation Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu, China
Wang Zhiming

Department of Neurosurgery, Affiliated Sichuan Provincial Rehabilitation Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu, China
Ma Xiankun

Department of Neurosurgery, Affiliated Sichuan Provincial Rehabilitation Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu, China
Zhang Hongdian

Neurosurgery Department, PLA Army General Hospital, Beijing, China

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APA Style

Pu Jiujun, Wang Zhiming, Ma Xiankun, Zhang Hongdian. (2021). Transdifferentiation of Human Umbilical Cord Stromal Cells to Neurogenic-Like Cells. Rehabilitation Science, 6(4), 76-82. https://doi.org/10.11648/j.rs.20210604.14

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ACS Style

Pu Jiujun; Wang Zhiming; Ma Xiankun; Zhang Hongdian. Transdifferentiation of Human Umbilical Cord Stromal Cells to Neurogenic-Like Cells. Rehabil. Sci. 2021, 6(4), 76-82. doi: 10.11648/j.rs.20210604.14

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AMA Style

Pu Jiujun, Wang Zhiming, Ma Xiankun, Zhang Hongdian. Transdifferentiation of Human Umbilical Cord Stromal Cells to Neurogenic-Like Cells. Rehabil Sci. 2021;6(4):76-82. doi: 10.11648/j.rs.20210604.14

Copy | Download

@article{10.11648/j.rs.20210604.14,
  author = {Pu Jiujun and Wang Zhiming and Ma Xiankun and Zhang Hongdian},
  title = {Transdifferentiation of Human Umbilical Cord Stromal Cells to Neurogenic-Like Cells},
  journal = {Rehabilitation Science},
  volume = {6},
  number = {4},
  pages = {76-82},
  doi = {10.11648/j.rs.20210604.14},
  url = {https://doi.org/10.11648/j.rs.20210604.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.rs.20210604.14},
  abstract = {Objective: To investigate the differentiation of human umbilical cord stromal cells into neurogenic cells. Methods: (1) Human umbilical cord stromal cells (HUMSCs) were pre-induced with recombinant human fibroblast growth factor-2 (hbFGF-2, Peprotech, UK) and N2 (1:100, Invitrogen, USA), immunocytochemistry and Western blot to determine the expression of nestin and fibronectin before and after pre-induction. Western blot to identify Nestin and fibronectin expression before and after pre-induction. (2) The differentiation of pre-induced and un-induced HUMSCs into neurogenic cells was performed, and the value-added rates of both were measured by MTT at different time points, and the morphological differences between the differentiated cells and the differentiated cells were verified at the gene and protein levels. Results:(1) 94.4 ± 2.3% and 6.5 ± 1.2% HUMSCs expressed ectodermal marker-fibronectin and stem cell marker-nestin, respectively, and 62.3 ± 5.2% piHUMSCs showed nestin immunopositive staining after adding FGF2 and N2 for pre-induction; 51.3 ± 5.9% of cells showed fibronectin immunopositive staining. piHUMSCs showed significantly higher nestin positivity than HUMSCs (P2 (1:100, Invitrogen, USA) were better able to differentiate into neural stem cells with stable expression of cell morphological changes, proteins and genes.},
 year = {2021}
}

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TY  - JOUR
T1  - Transdifferentiation of Human Umbilical Cord Stromal Cells to Neurogenic-Like Cells
AU  - Pu Jiujun
AU  - Wang Zhiming
AU  - Ma Xiankun
AU  - Zhang Hongdian
Y1  - 2021/12/20
PY  - 2021
N1  - https://doi.org/10.11648/j.rs.20210604.14
DO  - 10.11648/j.rs.20210604.14
T2  - Rehabilitation Science
JF  - Rehabilitation Science
JO  - Rehabilitation Science
SP  - 76
EP  - 82
PB  - Science Publishing Group
SN  - 2637-594X
UR  - https://doi.org/10.11648/j.rs.20210604.14
AB  - Objective: To investigate the differentiation of human umbilical cord stromal cells into neurogenic cells. Methods: (1) Human umbilical cord stromal cells (HUMSCs) were pre-induced with recombinant human fibroblast growth factor-2 (hbFGF-2, Peprotech, UK) and N2 (1:100, Invitrogen, USA), immunocytochemistry and Western blot to determine the expression of nestin and fibronectin before and after pre-induction. Western blot to identify Nestin and fibronectin expression before and after pre-induction. (2) The differentiation of pre-induced and un-induced HUMSCs into neurogenic cells was performed, and the value-added rates of both were measured by MTT at different time points, and the morphological differences between the differentiated cells and the differentiated cells were verified at the gene and protein levels. Results:(1) 94.4 ± 2.3% and 6.5 ± 1.2% HUMSCs expressed ectodermal marker-fibronectin and stem cell marker-nestin, respectively, and 62.3 ± 5.2% piHUMSCs showed nestin immunopositive staining after adding FGF2 and N2 for pre-induction; 51.3 ± 5.9% of cells showed fibronectin immunopositive staining. piHUMSCs showed significantly higher nestin positivity than HUMSCs (P2 (1:100, Invitrogen, USA) were better able to differentiate into neural stem cells with stable expression of cell morphological changes, proteins and genes.
VL  - 6
IS  - 4
ER  -

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