Review Article
Microscopy-based Data Processing in Cell Biology
Issue:
Volume 13, Issue 1, June 2025
Pages:
1-22
Received:
16 May 2025
Accepted:
3 June 2025
Published:
23 June 2025
DOI:
10.11648/j.cb.20251301.11
Downloads:
Views:
Abstract: By making it possible to extract intricate and significant biological information from visual imaging, data processing based on microscopy has completely changed contemporary cell biology. Researchers have overcome historical constraints by combining microscopy with sophisticated image processing technologies, opening up new possibilities for comprehending cellular architecture and functions in unprecedented detail. The goal of this study is to present a thorough examination of the methods and new developments in microscopy-driven data analysis, emphasizing both the theoretical underpinnings and real-world applications. The study starts by reviewing many microscopy techniques, including light, fluorescence, confocal, super-resolution, and electron microscopy, emphasizing their unique advantages and functions in contemporary cell biology. After that, it examines crucial picture preprocessing methods that are necessary for data dependability, such as contrast enhancement, background correction, and noise reduction. The segmentation and feature extraction techniques that allow precise cellular component detection and quantification are covered in detail. The article also describes new software and computational tools that facilitate automation and uniformity in the collection, processing, and analysis of images. The importance of quantitative analytic techniques for deciphering biological processes is also highlighted. These techniques include intensity measurement, colocalization, geographical distribution, and statistical analysis. Insights from microscope pictures are further improved by data visualization techniques like 3D rendering and machine learning software. The paper concludes by exploring emerging trends that have the potential to further change the field of microscopy in cell biology, including artificial intelligence, cloud-based platforms, multimodal imaging, and immersive technologies like augmented and virtual reality. To summarize, microscopy-based data processing is crucial to the advancement of cellular biology research, as it provides a wealth of opportunities for discovery through the integration of technology and multidisciplinary innovation.
Abstract: By making it possible to extract intricate and significant biological information from visual imaging, data processing based on microscopy has completely changed contemporary cell biology. Researchers have overcome historical constraints by combining microscopy with sophisticated image processing technologies, opening up new possibilities for compr...
Show More
,
Rojina Khatun
