Acid-Base Balance and Arterial Ion Concentrations in Rat Under Three Types of General Anaesthesia: Chronobiological Study

Pavol Svorc; Darina Petrasova; Pavol Svorc Jr.

doi:doi:10.11648/j.aap.20180301.14

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Acid-Base Balance and Arterial Ion Concentrations in Rat Under Three Types of General Anaesthesia: Chronobiological Study

Pavol Svorc, Darina Petrasova, Pavol Svorc Jr.

Published in Advances in Applied Physiology (Volume 3, Issue 1)

Received: 27 May 2018 Accepted: 8 June 2018 Published: 9 July 2018

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Abstract

The design and development of experimental, in vivo, chronobiological animal models may help reveal some of the relationships between circadian rhythms and biological functions. In vivo experiments require the use of appropriate anaesthesia, which should be selected according to their particular effect on the organism. The aim of study was to review the status of acid-base balance and ion concentration in arterial blood under common used general anaesthesias in experiments in dependence on the light-dark (LD) cycle in spontaneously breathing rats. The experiments were performed using three- to four-month-old pentobarbital (P)-, ketamine/xylazine (K/X)- and zoletil (Z)-aneasthetized female Wistar rats after a four-week adaptation to an LD cycle (12h light:12h dark). It was concluded that P anaesthesia disturbs LD dependence of acid-base balance compared to K/X and Z anaesthesia, but LD differences in plasma ion concentrations are disturbed under all type of general anaesthesia. P anaesthesia is not the most appropriate type of anaesthesia in rat chronobiological experiments. It eliminated LD differences, and also produces a more acidic environment, more pronounced hypercapnia and hypoxia than K/X and Z anaesthesias. This should be taken into account because the altered internal environment may affect the activity of systems whose functions are primarily dependent on acid-base balance or/and ion concentrations.

Published in	Advances in Applied Physiology (Volume 3, Issue 1)
DOI	10.11648/j.aap.20180301.14
Page(s)	26-32
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2018. Published by Science Publishing Group

Keywords

Chronobiology, Anaesthesia, Acid-Base Balance, Ions, Rats

References

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APA Style

Pavol Svorc, Darina Petrasova, Pavol Svorc Jr. (2018). Acid-Base Balance and Arterial Ion Concentrations in Rat Under Three Types of General Anaesthesia: Chronobiological Study. Advances in Applied Physiology, 3(1), 26-32. https://doi.org/10.11648/j.aap.20180301.14

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ACS Style

Pavol Svorc; Darina Petrasova; Pavol Svorc Jr. Acid-Base Balance and Arterial Ion Concentrations in Rat Under Three Types of General Anaesthesia: Chronobiological Study. Adv. Appl. Physiol. 2018, 3(1), 26-32. doi: 10.11648/j.aap.20180301.14

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AMA Style

Pavol Svorc, Darina Petrasova, Pavol Svorc Jr. Acid-Base Balance and Arterial Ion Concentrations in Rat Under Three Types of General Anaesthesia: Chronobiological Study. Adv Appl Physiol. 2018;3(1):26-32. doi: 10.11648/j.aap.20180301.14

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@article{10.11648/j.aap.20180301.14,
  author = {Pavol Svorc and Darina Petrasova and Pavol Svorc Jr.},
  title = {Acid-Base Balance and Arterial Ion Concentrations in Rat Under Three Types of General Anaesthesia: Chronobiological Study},
  journal = {Advances in Applied Physiology},
  volume = {3},
  number = {1},
  pages = {26-32},
  doi = {10.11648/j.aap.20180301.14},
  url = {https://doi.org/10.11648/j.aap.20180301.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.aap.20180301.14},
  abstract = {The design and development of experimental, in vivo, chronobiological animal models may help reveal some of the relationships between circadian rhythms and biological functions. In vivo experiments require the use of appropriate anaesthesia, which should be selected according to their particular effect on the organism. The aim of study was to review the status of acid-base balance and ion concentration in arterial blood under common used general anaesthesias in experiments in dependence on the light-dark (LD) cycle in spontaneously breathing rats. The experiments were performed using three- to four-month-old pentobarbital (P)-, ketamine/xylazine (K/X)- and zoletil (Z)-aneasthetized female Wistar rats after a four-week adaptation to an LD cycle (12h light:12h dark). It was concluded that P anaesthesia disturbs LD dependence of acid-base balance compared to K/X and Z anaesthesia, but LD differences in plasma ion concentrations are disturbed under all type of general anaesthesia. P anaesthesia is not the most appropriate type of anaesthesia in rat chronobiological experiments. It eliminated LD differences, and also produces a more acidic environment, more pronounced hypercapnia and hypoxia than K/X and Z anaesthesias. This should be taken into account because the altered internal environment may affect the activity of systems whose functions are primarily dependent on acid-base balance or/and ion concentrations.},
 year = {2018}
}

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TY  - JOUR
T1  - Acid-Base Balance and Arterial Ion Concentrations in Rat Under Three Types of General Anaesthesia: Chronobiological Study
AU  - Pavol Svorc
AU  - Darina Petrasova
AU  - Pavol Svorc Jr.
Y1  - 2018/07/09
PY  - 2018
N1  - https://doi.org/10.11648/j.aap.20180301.14
DO  - 10.11648/j.aap.20180301.14
T2  - Advances in Applied Physiology
JF  - Advances in Applied Physiology
JO  - Advances in Applied Physiology
SP  - 26
EP  - 32
PB  - Science Publishing Group
SN  - 2471-9714
UR  - https://doi.org/10.11648/j.aap.20180301.14
AB  - The design and development of experimental, in vivo, chronobiological animal models may help reveal some of the relationships between circadian rhythms and biological functions. In vivo experiments require the use of appropriate anaesthesia, which should be selected according to their particular effect on the organism. The aim of study was to review the status of acid-base balance and ion concentration in arterial blood under common used general anaesthesias in experiments in dependence on the light-dark (LD) cycle in spontaneously breathing rats. The experiments were performed using three- to four-month-old pentobarbital (P)-, ketamine/xylazine (K/X)- and zoletil (Z)-aneasthetized female Wistar rats after a four-week adaptation to an LD cycle (12h light:12h dark). It was concluded that P anaesthesia disturbs LD dependence of acid-base balance compared to K/X and Z anaesthesia, but LD differences in plasma ion concentrations are disturbed under all type of general anaesthesia. P anaesthesia is not the most appropriate type of anaesthesia in rat chronobiological experiments. It eliminated LD differences, and also produces a more acidic environment, more pronounced hypercapnia and hypoxia than K/X and Z anaesthesias. This should be taken into account because the altered internal environment may affect the activity of systems whose functions are primarily dependent on acid-base balance or/and ion concentrations.
VL  - 3
IS  - 1
ER  -

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Author Information

Pavol Svorc

Department of Physiology, Medical Faculty Safarik’s University, Kosice, Slovak Republic
Darina Petrasova

Laboratory of Research Bio-models, Medical Faculty Safarik’s University, Kosice, Slovak Republic
Pavol Svorc Jr.

Department of Physiology and Patophysiology, Medical Faculty Ostrava University, Ostrava, Czech Republic

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APA Style

Pavol Svorc, Darina Petrasova, Pavol Svorc Jr. (2018). Acid-Base Balance and Arterial Ion Concentrations in Rat Under Three Types of General Anaesthesia: Chronobiological Study. Advances in Applied Physiology, 3(1), 26-32. https://doi.org/10.11648/j.aap.20180301.14

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ACS Style

Pavol Svorc; Darina Petrasova; Pavol Svorc Jr. Acid-Base Balance and Arterial Ion Concentrations in Rat Under Three Types of General Anaesthesia: Chronobiological Study. Adv. Appl. Physiol. 2018, 3(1), 26-32. doi: 10.11648/j.aap.20180301.14

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AMA Style

Pavol Svorc, Darina Petrasova, Pavol Svorc Jr. Acid-Base Balance and Arterial Ion Concentrations in Rat Under Three Types of General Anaesthesia: Chronobiological Study. Adv Appl Physiol. 2018;3(1):26-32. doi: 10.11648/j.aap.20180301.14

Copy | Download

@article{10.11648/j.aap.20180301.14,
  author = {Pavol Svorc and Darina Petrasova and Pavol Svorc Jr.},
  title = {Acid-Base Balance and Arterial Ion Concentrations in Rat Under Three Types of General Anaesthesia: Chronobiological Study},
  journal = {Advances in Applied Physiology},
  volume = {3},
  number = {1},
  pages = {26-32},
  doi = {10.11648/j.aap.20180301.14},
  url = {https://doi.org/10.11648/j.aap.20180301.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.aap.20180301.14},
  abstract = {The design and development of experimental, in vivo, chronobiological animal models may help reveal some of the relationships between circadian rhythms and biological functions. In vivo experiments require the use of appropriate anaesthesia, which should be selected according to their particular effect on the organism. The aim of study was to review the status of acid-base balance and ion concentration in arterial blood under common used general anaesthesias in experiments in dependence on the light-dark (LD) cycle in spontaneously breathing rats. The experiments were performed using three- to four-month-old pentobarbital (P)-, ketamine/xylazine (K/X)- and zoletil (Z)-aneasthetized female Wistar rats after a four-week adaptation to an LD cycle (12h light:12h dark). It was concluded that P anaesthesia disturbs LD dependence of acid-base balance compared to K/X and Z anaesthesia, but LD differences in plasma ion concentrations are disturbed under all type of general anaesthesia. P anaesthesia is not the most appropriate type of anaesthesia in rat chronobiological experiments. It eliminated LD differences, and also produces a more acidic environment, more pronounced hypercapnia and hypoxia than K/X and Z anaesthesias. This should be taken into account because the altered internal environment may affect the activity of systems whose functions are primarily dependent on acid-base balance or/and ion concentrations.},
 year = {2018}
}

Copy | Download

TY  - JOUR
T1  - Acid-Base Balance and Arterial Ion Concentrations in Rat Under Three Types of General Anaesthesia: Chronobiological Study
AU  - Pavol Svorc
AU  - Darina Petrasova
AU  - Pavol Svorc Jr.
Y1  - 2018/07/09
PY  - 2018
N1  - https://doi.org/10.11648/j.aap.20180301.14
DO  - 10.11648/j.aap.20180301.14
T2  - Advances in Applied Physiology
JF  - Advances in Applied Physiology
JO  - Advances in Applied Physiology
SP  - 26
EP  - 32
PB  - Science Publishing Group
SN  - 2471-9714
UR  - https://doi.org/10.11648/j.aap.20180301.14
AB  - The design and development of experimental, in vivo, chronobiological animal models may help reveal some of the relationships between circadian rhythms and biological functions. In vivo experiments require the use of appropriate anaesthesia, which should be selected according to their particular effect on the organism. The aim of study was to review the status of acid-base balance and ion concentration in arterial blood under common used general anaesthesias in experiments in dependence on the light-dark (LD) cycle in spontaneously breathing rats. The experiments were performed using three- to four-month-old pentobarbital (P)-, ketamine/xylazine (K/X)- and zoletil (Z)-aneasthetized female Wistar rats after a four-week adaptation to an LD cycle (12h light:12h dark). It was concluded that P anaesthesia disturbs LD dependence of acid-base balance compared to K/X and Z anaesthesia, but LD differences in plasma ion concentrations are disturbed under all type of general anaesthesia. P anaesthesia is not the most appropriate type of anaesthesia in rat chronobiological experiments. It eliminated LD differences, and also produces a more acidic environment, more pronounced hypercapnia and hypoxia than K/X and Z anaesthesias. This should be taken into account because the altered internal environment may affect the activity of systems whose functions are primarily dependent on acid-base balance or/and ion concentrations.
VL  - 3
IS  - 1
ER  -

Copy | Download